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Apparent Dissociation Constant

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T Toyota – One of the best experts on this subject based on the ideXlab platform.

  • Relationship between glucocorticoid receptor and response to glucocorticoid therapy in ulcerative colitis.
    Diseases of The Colon & Rectum, 1997
    Co-Authors: T Shimada, N Hiwatashi, H Yamazaki, Y Kinouchi, T Toyota

    Abstract:

    PURPOSE: To clarify the relationship between the glucocorticoid receptor and the effectiveness of glucocorticoid therapy in patients with ulcerative colitis, we investigated the number and Apparent Dissociation Constant of glucocorticoid receptor in peripheral blood mononuclear leukocytes of patients with ulcerative colitis. MATERIALS AND METHODS: Eleven patients with ulcerative colitis (5 who responded to intravenous glucocorticoids and 6 who did not) and ten control subjects were studied. The number and Apparent Dissociation Constant of glucocorticoid receptor were measured using a whole-cell binding assay. Results were expressed as a median (interquartile range). RESULTS: The number of glucocorticoid receptors from the six nonresponders, five responders, and ten healthy controls were 4922 (range, 4484–5643), 3413 (range, 3183–4450), and 3610 (range, 2594–3979) binding sites/cell, respectively. The Apparent Dissociation Constant of the glucocorticoid receptors from the nonresponders, responders, and healthy controls were 7.03 (range, 5.66–10), 4.27 (range, 4–5.13), and 6.18 (range, 5.86–6.74) nM, respectively. Nonresponders had a significant increase both in the number of binding sites and in the Apparent Dissociation Constant compared with responders (P=0.045;P=0.029). CONCLUSIONS: The increased number and Apparent Dissociation Constant of glucocorticoid receptor are closely associated with the effectiveness of glucocorticoid therapy. The measurement of the number and Apparent Dissociation Constant of glucocorticoid receptor may be useful in predicting response to glucocorticoids.

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  • Relationship between glucocorticoid receptor and response to glucocorticoid therapy in ulcerative colitis.
    Diseases of the colon and rectum, 1997
    Co-Authors: T Shimada, N Hiwatashi, H Yamazaki, Y Kinouchi, T Toyota

    Abstract:

    To clarify the relationship between the glucocorticoid receptor and the effectiveness of glucocorticoid therapy in patients with ulcerative colitis, we investigated the number and Apparent Dissociation Constant of glucocorticoid receptor in peripheral blood mononuclear leukocytes of patients with ulcerative colitis.
    Eleven patients with ulcerative colitis (5 who responded to intravenous glucocorticoids and 6 who did not) and ten control subjects were studied. The number and Apparent Dissociation Constant of glucocorticoid receptor were measured using a whole-cell binding assay. Results were expressed as a median (interquartile range).
    The number of glucocorticoid receptors from the six nonresponders, five responders, and ten healthy controls were 4922 (range, 4484-5643), 3413 (range, 3183-4450), and 3610 (range, 2594-3979) binding sites/cell, respectively. The Apparent Dissociation Constant of the glucocorticoid receptors from the nonresponders, responders, and healthy controls were 7.03 (range, 5.66-10), 4.27 (range, 4-5.13), and 6.18 (range, 5.86-6.74) nM, respectively. Nonresponders had a significant increase both in the number of binding sites and in the Apparent Dissociation Constant compared with responders (P = 0.045; P = 0.029).
    The increased number and Apparent Dissociation Constant of glucocorticoid receptor are closely associated with the effectiveness of glucocorticoid therapy. The measurement of the number and Apparent Dissociation Constant of glucocorticoid receptor may be useful in predicting response to glucocorticoids.

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T Shimada – One of the best experts on this subject based on the ideXlab platform.

  • Relationship between glucocorticoid receptor and response to glucocorticoid therapy in ulcerative colitis.
    Diseases of The Colon & Rectum, 1997
    Co-Authors: T Shimada, N Hiwatashi, H Yamazaki, Y Kinouchi, T Toyota

    Abstract:

    PURPOSE: To clarify the relationship between the glucocorticoid receptor and the effectiveness of glucocorticoid therapy in patients with ulcerative colitis, we investigated the number and Apparent Dissociation Constant of glucocorticoid receptor in peripheral blood mononuclear leukocytes of patients with ulcerative colitis. MATERIALS AND METHODS: Eleven patients with ulcerative colitis (5 who responded to intravenous glucocorticoids and 6 who did not) and ten control subjects were studied. The number and Apparent Dissociation Constant of glucocorticoid receptor were measured using a whole-cell binding assay. Results were expressed as a median (interquartile range). RESULTS: The number of glucocorticoid receptors from the six nonresponders, five responders, and ten healthy controls were 4922 (range, 4484–5643), 3413 (range, 3183–4450), and 3610 (range, 2594–3979) binding sites/cell, respectively. The Apparent Dissociation Constant of the glucocorticoid receptors from the nonresponders, responders, and healthy controls were 7.03 (range, 5.66–10), 4.27 (range, 4–5.13), and 6.18 (range, 5.86–6.74) nM, respectively. Nonresponders had a significant increase both in the number of binding sites and in the Apparent Dissociation Constant compared with responders (P=0.045;P=0.029). CONCLUSIONS: The increased number and Apparent Dissociation Constant of glucocorticoid receptor are closely associated with the effectiveness of glucocorticoid therapy. The measurement of the number and Apparent Dissociation Constant of glucocorticoid receptor may be useful in predicting response to glucocorticoids.

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  • Relationship between glucocorticoid receptor and response to glucocorticoid therapy in ulcerative colitis.
    Diseases of the colon and rectum, 1997
    Co-Authors: T Shimada, N Hiwatashi, H Yamazaki, Y Kinouchi, T Toyota

    Abstract:

    To clarify the relationship between the glucocorticoid receptor and the effectiveness of glucocorticoid therapy in patients with ulcerative colitis, we investigated the number and Apparent Dissociation Constant of glucocorticoid receptor in peripheral blood mononuclear leukocytes of patients with ulcerative colitis.
    Eleven patients with ulcerative colitis (5 who responded to intravenous glucocorticoids and 6 who did not) and ten control subjects were studied. The number and Apparent Dissociation Constant of glucocorticoid receptor were measured using a whole-cell binding assay. Results were expressed as a median (interquartile range).
    The number of glucocorticoid receptors from the six nonresponders, five responders, and ten healthy controls were 4922 (range, 4484-5643), 3413 (range, 3183-4450), and 3610 (range, 2594-3979) binding sites/cell, respectively. The Apparent Dissociation Constant of the glucocorticoid receptors from the nonresponders, responders, and healthy controls were 7.03 (range, 5.66-10), 4.27 (range, 4-5.13), and 6.18 (range, 5.86-6.74) nM, respectively. Nonresponders had a significant increase both in the number of binding sites and in the Apparent Dissociation Constant compared with responders (P = 0.045; P = 0.029).
    The increased number and Apparent Dissociation Constant of glucocorticoid receptor are closely associated with the effectiveness of glucocorticoid therapy. The measurement of the number and Apparent Dissociation Constant of glucocorticoid receptor may be useful in predicting response to glucocorticoids.

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John J. Robinson – One of the best experts on this subject based on the ideXlab platform.

  • Biochemical analysis of a Ca2+‐dependent membrane–membrane interaction mediated by the sea urchin yolk granule protein, toposome
    Development growth & differentiation, 2006
    Co-Authors: Michael Hayley, Aruni Perera, John J. Robinson

    Abstract:

    Toposome, a high molecular mass protein, is an abundant component of the yolk granule in the sea urchin egg and embryo. Toposome is composed of a 160 kDa polypeptide that is proteolytically processed into smaller species of 120 and 90 kDa during embryonic development. The exact biological function of toposome during early development is unknown. In this study we have examined calcium binding to toposome and the effect of this binding on the secondary and tertiary structural characteristics of the purified protein. Initially, we used equilibrium dialysis to quantify calcium binding to toposome. Monophasic binding of up to 600 M of calcium per mole of protein was detected with an intrinsic Dissociation Constant (calcium) of 240 microm. Increasing concentrations of calcium resulted in an increase in alpha helical content from 3.0 to 22.0%, which occurred with an Apparent Dissociation Constant (calcium) of 25 microm. In parallel experiments, toposome binding to liposomes required similar concentrations of calcium; an Apparent Dissociation Constant (calcium) of 25 microm was recorded. Endogenous tryptophan fluorescence measurements, both in the presence and absence of liposomes, demonstrated that the tertiary structure is sensitive to increasing concentrations of calcium with an Apparent Dissociation Constant (calcium) of 240 microm. Toposome-driven, liposome aggregation assays revealed a similar calcium requirement. Collectively, these results define a two-step model for calcium modulation of toposome structure and function.

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