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Ralf T. Voegele – One of the best experts on this subject based on the ideXlab platform.

  • Characterization of a novel NADP(+)-dependent D-Arabitol dehydrogenase from the plant pathogen Uromyces fabae.
    The Biochemical journal, 2005
    Co-Authors: Tobias Link, Gertrud Lohaus, Ingrid Heiser, Kurt Mendgen, Matthias Hahn, Ralf T. Voegele

    Abstract:

    We have identified and characterized a novel NADP(+)-dependent D-Arabitol dehydrogenase and the corresponding gene from the rust fungus Uromyces fabae, a biotrophic plant pathogen on broad bean (Vicia faba). The new enzyme was termed ARD1p (D-Arabitol dehydrogenase 1). It recognizes D-Arabitol and mannitol as substrates in the forward reaction, and D-xylulose, D-ribulose and D-fructose as substrates in the reverse reaction. Co-factor specificity was restricted to NADP(H). Kinetic data for the major substrates and co-factors are presented. A detailed analysis of the organization and expression pattern of the ARD1 gene are also given. Immunocytological data indicate a localization of the gene product predominantly in haustoria, the feeding structures of these fungi. Analyses of metabolite levels during pathogenesis indicate that the D-Arabitol concentration rises dramatically as infection progresses, and D-Arabitol was shown in an in vitro system to be capable of quenching reactive oxygen species involved in host plant defence reactions. ARD1p may therefore play an important role in carbohydrate metabolism and in establishing and/or maintaining the biotrophic interaction in U. fabae.

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  • characterization of a novel nadp dependent d Arabitol dehydrogenase from the plant pathogen uromyces fabae
    Biochemical Journal, 2005
    Co-Authors: Tobias Link, Gertrud Lohaus, Ingrid Heiser, Kurt Mendgen, Matthias Hahn, Ralf T. Voegele

    Abstract:

    +-dependent D-Arabitol dehydrogenase and the corresponding gene from the rustfungusUromycesfabae,abiotrophicplantpathogenonbroad bean (Vicia faba). The new enzyme was termed ARD1p (DArabitol dehydrogenase 1). It recognizes D-Arabitol and mannitol as substrates in the forward reaction, and D-xylulose, D-ribulose and D-fructose as substrates in the reverse reaction. Co-factor specificity was restricted to NADP(H). Kinetic data for the major substrates and co-factors are presented. A detailed analysis of the organization and expression pattern of the ARD1 gene are also given. Immunocytological data indicate a localization of the gene product predominantly in haustoria, the feeding structures of these fungi. Analyses of metabolite levels during pathogenesis indicate that the D-Arabitol concentration rises dramatically as infection progresses, and D-Arabitol was shown in an invitrosystemtobecapableofquenchingreactiveoxygenspecies involved in host plant defence reactions. ARD1p may therefore play an important role in carbohydrate metabolism and in establishing and/or maintaining the biotrophic interaction in U. fabae.

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  • Characterization of a novel NADP(+)-dependent D-Arabitol dehydrogenase from the plant pathogen Uromyces fabae.
    Biochemical Journal, 2005
    Co-Authors: Tobias Link, Gertrud Lohaus, Ingrid Heiser, Kurt Mendgen, Matthias Hahn, Ralf T. Voegele

    Abstract:

    +-dependent D-Arabitol dehydrogenase and the corresponding gene from the rustfungusUromycesfabae,abiotrophicplantpathogenonbroad bean (Vicia faba). The new enzyme was termed ARD1p (DArabitol dehydrogenase 1). It recognizes D-Arabitol and mannitol as substrates in the forward reaction, and D-xylulose, D-ribulose and D-fructose as substrates in the reverse reaction. Co-factor specificity was restricted to NADP(H). Kinetic data for the major substrates and co-factors are presented. A detailed analysis of the organization and expression pattern of the ARD1 gene are also given. Immunocytological data indicate a localization of the gene product predominantly in haustoria, the feeding structures of these fungi. Analyses of metabolite levels during pathogenesis indicate that the D-Arabitol concentration rises dramatically as infection progresses, and D-Arabitol was shown in an invitrosystemtobecapableofquenchingreactiveoxygenspecies involved in host plant defence reactions. ARD1p may therefore play an important role in carbohydrate metabolism and in establishing and/or maintaining the biotrophic interaction in U. fabae.

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Wenjing Sun – One of the best experts on this subject based on the ideXlab platform.

  • Optimization of fermentation medium for a newly isolated yeast strain (Zygosaccharomyces rouxii JM-C46) and evaluation of factors affecting biosynthesis of D-Arabitol
    LWT, 2019
    Co-Authors: Qi Guo, Xu Wang, Huanhuan Zhang, Hossain M. Zabed, Junhua Yun, Guoyan Zhang, Miaomiao Yang, Wenjing Sun

    Abstract:

    Abstract A newly isolated yeast strain, namely Zygosaccharomyces rouxii JM-C46, was reported to be promising for enhanced D-Arabitol production from glucose. In this study, further efforts were made to optimize fermentation conditions for this strain through one-factor-at-a-time strategy considering seven important factors of shake flask fermentation. Subsequently, composition of fermentation medium was optimized using Box-Behnken design (BBD) of response surface methodology (RSM) including three major nitrogen providing ingredients, namely yeast extract, (NH4)2SO4 and peptone. Maximum D-Arabitol concentration produced in one-factor-at-a-time experiments was found to be 72.69 g/L under the optimum conditions consisting of 200 g/L initial glucose, 100 ml medium, 5% (equivalent to 8.78 g/L) initial inoculum, temperature 30 °C, pH 5.0, fermentation time 96 h, and agitation rate 200 rpm. In the experiments of BBD, the predicted yield of D-Arabitol varied between 67.73 g/L and 76.76 g/L, while the actual concentration of D-Arabitol ranged from 68.02 g/L to 76.95 g/L. The optimum composition of medium F (final medium) contained d -glucose (200 g/L), yeast extract (10 g/L), (NH4)2SO4 (2 g/L) and peptone (7.5 g/L), which further increased D-Arabitol production from 72.69 g/L to 76.32 g/L.

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  • Enhanced d-Arabitol production by Zygosaccharomyces rouxii JM-C46: isolation of strains and process of repeated-batch fermentation
    Journal of Industrial Microbiology & Biotechnology, 2015
    Co-Authors: Yan Luo, Xu Wang, Jingfei Zhu, Jing Lin, Huanhuan Zhang, Fang Chen, Wenjing Sun

    Abstract:

    A new strain producing high yield of d-Arabitol was isolated from hyperosmotic environments and the ITS rDNA sequencing analysis revealed it as Zygosaccharomyces rouxii. In addition, using a pH control and repeated-batch fermentation strategy in a 5-L reactor, the maximum yield and the highest volumetric productivity of d-Arabitol were 93.48 ± 2.79 g/L and 1.143 g/L h, respectively. Volumetric productivity was successfully improved from 0.86 to 1.143 g/L h, which was increased by 32.9 % after 72 h of fermentation. Z. rouxii JM-C46 has potential to be used for d-Arabitol and xylitol production from glucose via d-Arabitol route.

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Y. S. Li – One of the best experts on this subject based on the ideXlab platform.

  • contribution of fungal spores to particulate matter in a tropical rainforest
    Environmental Research Letters, 2010
    Co-Authors: Ting Zhang, Guenter Engling, Chuenyu Chan, Yinan Zhang, Zhisheng Zhang, Xuefang Sang, Yd D Li, Y. S. Li

    Abstract:

    The polyols Arabitol and mannitol, recently proposed as source tracers for fungal spores, were used in this study to estimate fungal contributions to atmospheric aerosol. Airborne particulate matter (PM2.5 and PM10) was collected at Jianfengling Mountain, a tropical rainforest on Hainan Island situated off the south China coast, during spring and analyzed for Arabitol and mannitol by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The average concentrations of Arabitol and mannitol exhibited high values with averages of 7.0 and 16.0  ng m − 3 respectively in PM2.5 and 44.0 and 71.0 ng m − 3 in PM10. The two tracers correlated well with each other, especially in the coarse mode aerosol (PM2.5 − 10), indicating they were mainly associated with coarse aerosol particles and had common sources. Arabitol and mannitol in PM10 showed significant positive correlations with relative humidity, as well as positive correlations with average temperature, suggesting a wet emissions mechanism of biogenic aerosol in the form of fungal spores. We made estimations of the contribution of fungal spores to ambient PM mass and to organic carbon, based on the observed ambient concentrations of these two tracers. The relative contributions of fungal spores to the PM10 mass were estimated to range from 1.6 to 18.2%, with a rather high mean value of 7.9%, and the contribution of fungal spores to organic carbon in PM10 ranged from 4.64 to 26.1%, with a mean value of 12.1%, implying that biological processes are important sources of atmospheric aerosol.

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