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Aralia

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Haixue Kuang – 1st expert on this subject based on the ideXlab platform

  • simultaneous determination of four triterpenoid saponins in Aralia elata leaves by hplc elsd combined with hierarchical clustering analysis
    Phytochemical Analysis, 2017
    Co-Authors: Baimei Li, Zhibin Wang, Qiuhong Wang, Hongwei Zhang, Hai Jiang, Haixue Kuang

    Abstract:

    Introduction
    Aralia elata leaves are known to have several biological activities, including anti-arrythmia, antitumor, anti-inflammatory, anti-fatigue, antimicrobial and antiviral effects. Our previous study found that triterpenoid saponins from the leaves of A. elata had antitumor effects. Quantification of the triterpenoids is important for the quality control of A. elata leaves.

    Objective
    To establish high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) for the simultaneous determination of four major triterpenoid saponins, including Aralia-saponin IV, Aralia-saponin VI, 3-O-β-d- glucopyranosyl-(1 → 3)-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside (Aralia-saponin TTP)and Aralia-saponin V.

    Methodology
    The separation was carried out on a Dikma Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) efficiently with gradient elution consisting of acetonitrile and water.

    Results
    All calibration curves showed good linear regression (R2 > 0.9996) within the ranges of tested concentrations. This validated method was applied to determine the contents of the four major triterpenoid saponins in 53 samples from different regions of northeast China. Hierarchical clustering analysis was first used to classify and differentiate Aralia elata leaves.

    Conclusion
    The method developed was successfully applied to analyse four major triterpenoid saponins in Aralia elata leaves which is helpful for quality control of the herb. Copyright © 2017 John Wiley & Sons, Ltd.

  • determination and pharmacokinetic study of two triterpenoid saponins in rat plasma after oral administration of the extract of Aralia elata leaves by uhplc esi ms ms
    Journal of Chromatography B, 2015
    Co-Authors: Zhibin Wang, Qiong Wu, Yonghai Meng, Qi Wang, Chunjuan Yang, Qiuhong Wang, Bingyou Yang, Haixue Kuang

    Abstract:

    Abstract Aralia elata (Miq.) Seems (A. elata) grow in Northeast China and the total saponins of A. elata is used to auxiliary treatment for the acute hepatitis, chronic hepatitis and the transaminase on the high side. Aralia-saponinV and Aralia-saponinVI are the major bioactive saponins in A. elata leaves. A selective and sensitive UHPLC–MS/MS method was developed and validated for the determination and pharmacokinetic study of Aralia-saponinV and Aralia-saponinVI indwelling the extract in rat plasma in this article. The sample pretreatment involved a one-step extraction of 0.2 mL plasma with methanol. Shengmaxinside C was used as internal standard (I.S.). The separation was carried out on an Agilent SB-C18 column (1.8 μm, 50 mm × 2.1 mm) at 30 °C with a mobile phase of acetonitrile–5 mM ammonium acetate (90:10, v/v) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source operating in the negative ionization mode. The optimized mass transition ion-pairs (m/z) monitored for Aralia-saponinV, Aralia-saponinVI and I.S. were 1103.2/941.2, 1119.2/957.0 and 707.0/647.1, respectively. The current UHPLC–MS/MS assay method was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability, and it was suitable for the pharmacokinetic studies of the two saponins after oral administration of extract of A. elata leaves. The lower limits of quantification were 5.70 ng/mL for Aralia-saponinV and 6.15 ng/mL for Aralia-saponinVI. Intra-day and inter-day precisions were less than 7.4% and the accuracy range was from 1.19% to 8.60%. The mean extraction recoveries of analytes and I.S. from rat plasma were all more than 89.5%. This paper described a simple, sensitive and validated UHPLC–MS/MS method for simultaneous determination of Aralia-saponinV and Aralia-saponinVI in rat plasma after oral administration of the extract of A. elata leaves, and investigated on their pharmacokinetic studies as well.

Zhibin Wang – 2nd expert on this subject based on the ideXlab platform

  • simultaneous determination of four triterpenoid saponins in Aralia elata leaves by hplc elsd combined with hierarchical clustering analysis
    Phytochemical Analysis, 2017
    Co-Authors: Baimei Li, Zhibin Wang, Qiuhong Wang, Hongwei Zhang, Hai Jiang, Haixue Kuang

    Abstract:

    Introduction
    Aralia elata leaves are known to have several biological activities, including anti-arrythmia, antitumor, anti-inflammatory, anti-fatigue, antimicrobial and antiviral effects. Our previous study found that triterpenoid saponins from the leaves of A. elata had antitumor effects. Quantification of the triterpenoids is important for the quality control of A. elata leaves.

    Objective
    To establish high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) for the simultaneous determination of four major triterpenoid saponins, including Aralia-saponin IV, Aralia-saponin VI, 3-O-β-d- glucopyranosyl-(1 → 3)-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside (Aralia-saponin TTP)and Aralia-saponin V.

    Methodology
    The separation was carried out on a Dikma Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) efficiently with gradient elution consisting of acetonitrile and water.

    Results
    All calibration curves showed good linear regression (R2 > 0.9996) within the ranges of tested concentrations. This validated method was applied to determine the contents of the four major triterpenoid saponins in 53 samples from different regions of northeast China. Hierarchical clustering analysis was first used to classify and differentiate Aralia elata leaves.

    Conclusion
    The method developed was successfully applied to analyse four major triterpenoid saponins in Aralia elata leaves which is helpful for quality control of the herb. Copyright © 2017 John Wiley & Sons, Ltd.

  • determination and pharmacokinetic study of two triterpenoid saponins in rat plasma after oral administration of the extract of Aralia elata leaves by uhplc esi ms ms
    Journal of Chromatography B, 2015
    Co-Authors: Zhibin Wang, Qiong Wu, Yonghai Meng, Qi Wang, Chunjuan Yang, Qiuhong Wang, Bingyou Yang, Haixue Kuang

    Abstract:

    Abstract Aralia elata (Miq.) Seems (A. elata) grow in Northeast China and the total saponins of A. elata is used to auxiliary treatment for the acute hepatitis, chronic hepatitis and the transaminase on the high side. Aralia-saponinV and Aralia-saponinVI are the major bioactive saponins in A. elata leaves. A selective and sensitive UHPLC–MS/MS method was developed and validated for the determination and pharmacokinetic study of Aralia-saponinV and Aralia-saponinVI indwelling the extract in rat plasma in this article. The sample pretreatment involved a one-step extraction of 0.2 mL plasma with methanol. Shengmaxinside C was used as internal standard (I.S.). The separation was carried out on an Agilent SB-C18 column (1.8 μm, 50 mm × 2.1 mm) at 30 °C with a mobile phase of acetonitrile–5 mM ammonium acetate (90:10, v/v) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source operating in the negative ionization mode. The optimized mass transition ion-pairs (m/z) monitored for Aralia-saponinV, Aralia-saponinVI and I.S. were 1103.2/941.2, 1119.2/957.0 and 707.0/647.1, respectively. The current UHPLC–MS/MS assay method was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability, and it was suitable for the pharmacokinetic studies of the two saponins after oral administration of extract of A. elata leaves. The lower limits of quantification were 5.70 ng/mL for Aralia-saponinV and 6.15 ng/mL for Aralia-saponinVI. Intra-day and inter-day precisions were less than 7.4% and the accuracy range was from 1.19% to 8.60%. The mean extraction recoveries of analytes and I.S. from rat plasma were all more than 89.5%. This paper described a simple, sensitive and validated UHPLC–MS/MS method for simultaneous determination of Aralia-saponinV and Aralia-saponinVI in rat plasma after oral administration of the extract of A. elata leaves, and investigated on their pharmacokinetic studies as well.

Shaojiang Song – 3rd expert on this subject based on the ideXlab platform

  • The resource investigation and microscopic identification of cortex of Aralia elata
    Journal of Shenyang Pharmaceutical University, 2020
    Co-Authors: Jincai Lu, Shaojiang Song, Xinjiang Wang

    Abstract:

    The resource distribution of Aralia elata in northeast China were investigated. By means of the optical microscope, the features of histology and powder of cortex of this plant were first examined. In the cortex, there are a large number of secretory ducts and a small quantity of druses of calcium oxalate.

  • effect of five triterpenoid compounds from the buds of Aralia elata on stimulus induced superoxide generation tyrosyl phosphorylation and translocation of cytosolic compounds to the cell membrane in human neutrophils
    Planta Medica, 2006
    Co-Authors: Mei Zhang, Shaojiang Song, Shuhan Tang, Koichi Yamashita, Masanobu Manabe, Hiroyuki Kodama

    Abstract:

    The buds of Aralia elata (Miq.) Seem (Japanese angelica tree) have long been used as a tonic, antiarthritic and antidiabetic agent in China and Japan. We have isolated five triterpenoids, congmuyanosides A, C, D, echinocystic acid and 3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-hederagenin from the buds of Aralia elata, and investigated their effects on stimulus-induced superoxide generation in human neutrophils. Congmuyanoside A, echinocystic acid and 3-O-[β-D-glucopyranosyl(12)-β-D-glucopyranosyl]-hederagenin suppressed the superoxide generation induced by N-formyl-methionyl-leucylphenylalanine (fMLP) in a concentration-dependent manner. Congmuyanosides C, D and echinocystic acid significantly suppressed the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA) and arachidonic acid (AA). The compounds also suppressed fMLP- and AA-induced tyrosyl or PMA-induced serine/threonine phosphorylation and translocation of cytosolic compounds, p47 phox , p67 phox and Rac to the cell membrane.

  • five saponins from the root bark of Aralia elata
    Phytochemistry, 2001
    Co-Authors: Shaojiang Song, Norio Nakamura, Masao Hattori, Suixu Xu

    Abstract:

    Abstract Five saponins, 3-O-{β- d -glucopyranosyl (1→2)-[β- d -glucopyranosyl (1→3)]-β- d -glucopyranosyl}-oleanolic acid 28-O-β- d -glucopyranosyl ester (Aralia-saponin V), 3-O-{β- d -glucopyranosyl (1→2)-[β- d -glucopyranosyl (1→3)]-β- d -glucopyranosyl}-echinocystic acid 28-O-β- d -glucopyranosyl ester (Aralia-saponin VI), 3-O-{β- d -glucopyranosyl (1→2)-[β- d -glucopyranosyl (1→3)]-β- d -glucopyranosyl}-hederagenin 28-O-β- d -glucopyranosyl ester (Aralia-saponin VII), 3-O-{β- d -glucopyranosyl-(1→3)-β- d -glucopyranosyl-(1→ 3)-[β- d -glucopyranosyl-(1→2)]-β- d -glucopyranosyl}-caulophyllogenin 28-O-β- d -glucopyranosyl ester (Aralia-saponin VIII), 3-O-{β- d -glucopyranosyl (1→2)-[β- d -glucopyranosyl(1→3)]-α- l -arabinopyranosyl}-hederagenin 28-O-β- d -glucopyranosyl ester (Aralia-saponin IX), were isolated from the root bark of Aralia elata (Miq.) Seem., together with four known compounds. Their structures were determined on the basis of chemical and spectroscopic methods.