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Felipe Vázquez-flota - One of the best experts on this subject based on the ideXlab platform.

Jorge Rubio-piña - One of the best experts on this subject based on the ideXlab platform.

  • Pharmaceutical applications of the benzylisoquinoline alkaloids from Argemone Mexicana L.
    Current Topics in Medicinal Chemistry, 2013
    Co-Authors: Jorge Rubio-piña, Felipe Vázquez-flota, Jorge Rubio-piña, Felipe Vázquez-flota
    Abstract:

    Argemone Mexicana L. (Papaveraceae), accumulates benzylisoquinoline alkaloids, (BIA) derived from tyrosine. Although it was originated in the western region of the USA-Mexico border, it has spread to tropical and subtropical areas around the world. Today, it is used to treat different ailments, given to its antimicrobial, antiparasitic, antimalarial, pesticide, cytotoxic and neurological properties. These effects are related to the presence of different types of BIA's, such as benzophenanthridines (sanguinarine, chelerythrine), protoberberines (berberine) and protopines (protopine, allocryptopine). This review covers the historical medicinal uses of A. Mexicana, as well as its current applications. The chemical bases of such effects are discussed in relation to the occurrence of the different types of alkaloids. The biochemical process involved in the synthesis of these types of alkaloids is also described.

  • Establishment of a sanguinarine-producing cell suspension culture of Argemone Mexicana L (Papaveraceae): induction of alkaloid accumulation.
    Methods in molecular biology (Clifton N.J.), 2012
    Co-Authors: Felipe Vázquez-flota, Cecilia Guízar-gonzález, Miriam Monforte-gonzález, Jorge Rubio-piña, Karen Trujillo-villanueva
    Abstract:

    A protocol for the induction of a cell suspension culture of Argemone Mexicana is described. This suspension has been kept for over 3 years producing sanguinarine, a benzophenanthridine-type alkaloid. Sanguinarine levels can be increased by exposing these cultures to yeast or fungal elicitation.

  • Fusarium oxysporum homogenates and jasmonate induce limited sanguinarine accumulation in Argemone Mexicana cell cultures
    Biotechnology Letters, 2010
    Co-Authors: Karen Trujillo-villanueva, Miriam Monforte-gonzález, Jorge Rubio-piña, Felipe Vázquez-flota
    Abstract:

    In vitro cultures of Argemone Mexicana (Papaveraceae) were induced from leaves of mature plants. Sanguinarine, a benzophenanthridine, was the main alkaloid in the cultures, even in the absence of inducers of secondary metabolism. The accumulation of this metabolite was increased by adding methyl jasmonate and fungal elicitors, although in a limited fashion in comparison to other sanguinarine-producing species. Evidence of a transport mechanism, which may be related to the magnitude of the response, was obtained based on the fluorescent properties of bezophenathridines in the elicited cultures.

  • Isolation of functional total RNA from Argemone Mexicana tissues
    Electronic Journal of Biotechnology, 2008
    Co-Authors: Jorge Rubio-piña, Felipe Vázquez-flota
    Abstract:

    RNA extraction from recalcitrant plant tissues is frequently complicated by the presence of secondary metabolites, polysaccharides and polyphenols. These compounds may co precipitate with RNA, often rendering it unsuitable for either cDNA synthesis or hybridization in northern blot analyses and therefore, interfering with the gene analysis expression in such tissues. We have developed an efficient RNA extraction method from A. Mexicana tissues. The procedure includes the use of polyvinylpolypyrrolidone (PVPP), to remove secondary metabolites, proteins and polyphenols, and a two-step precipitation with LiCl, to eliminate polysaccharides, and thus increasing RNA yield. The quality of the resulting RNA was evaluated spectrophotometrically and by agarose gel electrophoresis. Moreover, the RNA obtained by this method, could be used directly for both RT-PCR and northern blot analysis, without any further purification. Argemone Mexicana (Papaveraceae), commonly known as prickly poppy is used in rural areas of Mexico as a medicinal plant. The occurrence of diverse alkaloids, flavonoids and fatty acids in its tissues may explain these properties (Shaukat et al. 2002; Chang et al. 2003). In fact, berberine and sanguinarine, two of the main alkaloids isolated from Argemone tisues , display significant

Karen Trujillo-villanueva - One of the best experts on this subject based on the ideXlab platform.

Miriam Monforte-gonzález - One of the best experts on this subject based on the ideXlab platform.

Cecilia Guízar-gonzález - One of the best experts on this subject based on the ideXlab platform.