The Experts below are selected from a list of 2040 Experts worldwide ranked by ideXlab platform
Azucena Gonzalezcoloma - One of the best experts on this subject based on the ideXlab platform.
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supercritical anti solvent fractionation of Artemisia Absinthium l conventional extracts tracking artemetin and casticin
Journal of Supercritical Fluids, 2019Co-Authors: Elisa Langa, Azucena Gonzalezcoloma, Juan I Pardo, Carlota Gimenezrota, Maria J Hernaiz, Ana M MainarAbstract:Abstract In the current work the Supercritical Anti-solvent Fractionation (SAF) methodology was applied to conventional extracts from Artemisia Absinthium L. (wormwood). Artemetin and casticin, two compounds found in wormwood extracts and with high structural similarity, were tracked in all the experiments. A Response Surface Methodology (RSM), based on Central Composite Design (CCD) was used for both the experimental design and the results correlation. Studied variables were pressure (from 8.0–15.0 MPa) and CO2 flow rate (from 10 to 60 g·min−1), while temperature (40 °C) and feed solution flow rate (0.45 mL·min−1) were maintained constant. Overall achieved yields were around 70%, being downstream vessel yields much higher than those in the precipitation vessel. The conditions predicted to reach an optimal overall yield and fractionation were 80 MPa and 10 g·min−1 (composite desirability = 0.7443). Casticin and artemetin were mainly obtained in the downstream vessel, being this fraction more enriched in artemetin than casticin.
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absolute configuration of the ocimene monoterpenoids from Artemisia Absinthium
Chirality, 2017Co-Authors: Luis F Julio, Azucena Gonzalezcoloma, Carmen E Diaz, Eleuterio Burguenotapia, Nury Perezhernandez, Pedro JosephnathanAbstract:: The absolute configuration (AC) of the naturally occurring ocimenes (-)-(3S,5Z)-2,6-dimethyl-2,3-epoxyocta-5,7-diene (1) and (-)-(3S,5Z)-2,6-dimethylocta-5,7-dien-2,3-diol (2), isolated from the essential oils of domesticated specimens of Artemisia Absinthium, followed by vibrational circular dichroism (VCD) studies of 1, as well as from the acetonide 3 and the monoacetate 4, both derived from 2, since secondary alcohols are not the best functional groups to be present during VCD studies in solution due to intermolecular associations. The AC follows from comparison of experimental and calculated VCD spectra that were obtained by Density Functional Theory computation at the B3LYP/DGDZVP level of theory. Careful nuclear magnetic resonance (NMR) measurements were compared with literature values, providing for the first time systematic 1 H and 13 C chemical shift data. Regarding homonuclear 1 H coupling constants, after performing a few irradiation experiments that showed the presence of several small long-range interactions, the complete set of coupling constants for 3, which is representative of the four studied molecules, was determined by iterations using the PERCH software. This procedure even allowed assigning the pro-R and pro-S methyl group signals of the two gem-dimethyl groups present in 3.
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chemical and biocidal characterization of two cultivated Artemisia Absinthium populations with different domestication levels
Industrial Crops and Products, 2015Co-Authors: Luis F Julio, Carmen E Diaz, Raimundo Cabrera, Jesus Burillo, Cristina Gimenez, Jesus Sanz, Azucena GonzalezcolomaAbstract:Abstract The objective of this study was the characterization and valorization of the essential oil from a domesticated Artemisia Absinthium population (Teruel, Spain), and its comparison with another one (Sierra Nevada, Spain) undergoing the domestication process. These populations are being experimentally cultivated in the same field since 2008. We studied their biomass and essential oil production (Clevenger hydrodistillation, HD and semi-industrial vapor-pressure, VP). The domesticated population showed lower chemical variation and higher biomass and essential oil yields, allowing for the registration of a new plant variety. The observed variations in oil composition (HD, VP) between the two populations were mostly quantitative. The oils were characterized by the presence of cis-epoxyocimene, (−)-cis-chrysanthenol, chrysanthenyl acetate, linalool and trans-caryophyllene. The insect antifeedant (Leptinotarsa decemlineata, Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi) and antifungal (Fusarium spp. and Botrytis cinerea) effects of their oils were also tested. All VP extracts showed strong antifungal effects and the active antifungal compounds have been identified.
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selective nematocidal effects of essential oils from two cultivated Artemisia Absinthium populations
Zeitschrift für Naturforschung C, 2015Co-Authors: Juan Jose Garciarodriguez, Jesus Burillo, Maria Fe Andres, Alexandra Ibanezescribano, Luis F Julio, F Bolasfernandez, Azucena GonzalezcolomaAbstract:: Essential oils (EOs) obtained from two crops and populations of thujone-free cultivated Artemisia Absinthium were tested against two nematode models, the mammalian parasite Trichinella spiralis, and the plant parasitic root knot nematode Meloidogyne javanica. The EOs were characterized by the presence of (Z)-epoxyocimene and chrysanthenol as major components and showed time and population dependent quantitative and qualitative variations in composition. The EOs showed a strong ex vivo activity against the L1 larvae of the nematode Trichinella spiralis with a reduction of infectivity between 72 and 100% at a dose range of 0.5-1 mg/ml in absence of cytotoxicity against mammalian cells. Moreover, the in vivo activity of the EO against T. spiralis showed a 66% reduction of intestinal adults. However, these oils were not effective against M. javanica.
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major components of spanish cultivated Artemisia Absinthium populations antifeedant antiparasitic and antioxidant effects
Industrial Crops and Products, 2012Co-Authors: Azucena Gonzalezcoloma, Maria Bailen, Carmen E Diaz, Braulio M Fraga, Rafael A Martinezdiaz, Gustavo E Zuniga, Rodrigo A Contreras, Raimundo Cabrera, Jesus BurilloAbstract:Abstract The objective of this study was the valorization of Spanish Artemisia Absinthium populations from Teruel (Aragon) and Sierra Nevada (Granada). These populations were experimentally cultivated in the field and under controlled conditions. Three major components were isolated from a two year-old population obtained from the Teruel population cultivated in Ejea-Zaragoza in 2003, and identified by NMR experiments as the sequiterpene lactone hydroxypelenolide (I) and the flavones artemetin (II) and casticin (III). The I–III content of the plant extracts was analyzed by HPLC-DAD. The insect antifeedant properties of plant extracts from different years and crops were tested against Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi. Additionally we studied their antioxidant, phytotoxic and antiparasitic effects.
Peter G Waterman - One of the best experts on this subject based on the ideXlab platform.
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volatile oils from normal and transformed root of Artemisia Absinthium
Phytochemistry, 1993Co-Authors: Alan I Kennedy, S G Deans, Katerina P Svoboda, Alexander I Gray, Peter G WatermanAbstract:Abstract The volatile oil distilled from the roots of field-grown Artemisia Absinthium and from fermenter grown hairy roots of A. Absinthium transformed
R Magherini - One of the best experts on this subject based on the ideXlab platform.
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agrobacterium mediated transformation of Artemisia Absinthium l wormwood and production of secondary metabolites
Plant Cell Reports, 1997Co-Authors: Andrea Bennici, S Schiff, G Roselli, D Mariotti, R MagheriniAbstract:Hairy roots were obtained after infection of Artemisia Absinthium shoots with Agrobacterium rhizogenes strains 1855 and LBA 9402. The susceptibility to hairy root transformation varied between plant genotypes and bacterial strains. Hairy roots showed macroscopic differences from control root cultures. Southern blot hybridization confirmed the integration of T-DNA from both p1855 and pBin19, while polymerase chain reaction analysis indicated the presence of the neomycin phosphotransferase gene in the hairy root genome. Subcultured transformed root lines grew well in selective B5 agar-solidified medium containing kanamycin or rifampicin and without hormones. Shake-flask experiments with fast-growing root lines showed that 40 g l–1 was the best sucrose concentration for biomass production, yielding a 463-fold increase in dry weight after 28 days of culture. Great differences were found in the profiles of the essential oils isolated from normal and hairy roots. Gas chromatography/mass spectrometry analysis showed the oil produced by transformed cultures to be a mixture of 50 compounds with only one major component representing 37% of the oil content.
Andrea Bennici - One of the best experts on this subject based on the ideXlab platform.
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agrobacterium mediated transformation of Artemisia Absinthium l wormwood and production of secondary metabolites
Plant Cell Reports, 1997Co-Authors: Andrea Bennici, S Schiff, G Roselli, D Mariotti, R MagheriniAbstract:Hairy roots were obtained after infection of Artemisia Absinthium shoots with Agrobacterium rhizogenes strains 1855 and LBA 9402. The susceptibility to hairy root transformation varied between plant genotypes and bacterial strains. Hairy roots showed macroscopic differences from control root cultures. Southern blot hybridization confirmed the integration of T-DNA from both p1855 and pBin19, while polymerase chain reaction analysis indicated the presence of the neomycin phosphotransferase gene in the hairy root genome. Subcultured transformed root lines grew well in selective B5 agar-solidified medium containing kanamycin or rifampicin and without hormones. Shake-flask experiments with fast-growing root lines showed that 40 g l–1 was the best sucrose concentration for biomass production, yielding a 463-fold increase in dry weight after 28 days of culture. Great differences were found in the profiles of the essential oils isolated from normal and hairy roots. Gas chromatography/mass spectrometry analysis showed the oil produced by transformed cultures to be a mixture of 50 compounds with only one major component representing 37% of the oil content.
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callus cultures of Artemisia Absinthium l initiation growth optimization and organogenesis
Plant Cell Tissue and Organ Culture, 1996Co-Authors: Elena Morosi, S Schiff, Andrea BenniciAbstract:Callus cultures were initiated from micropropagated Artemisia Absinthium plantlets on MS basal medium supplemented with different concentrations of BA, Kn, NAA, IAA and 2,4-d in combination or singly. Supplementing the medium with low doses of both BA in combination with NAA, and Kn in combination with NAA enhanced the growth rate of callus cultures. However, cultures grew slowly following the second subculture and the majority turned brown and died within the next month. Initiation of root and shoot primordia occured directly from leaf explants cultured on 1.81 μM 2,4-d, while adventitious shoot formation from callus was observed occasionally when BA was added to the medium in combination with IAA. Furthermore, medium containing 2.22 μM BA and 2.69 μM NAA stimulated both callus growth and organogenesis on some callus cultures derived from leaves and stems of young stock material. The best results were obtained with leaf explants. Cytological analysis of root meristems revealed that all regenerants were diploid (2n=18), as expected.
Ana M Mainar - One of the best experts on this subject based on the ideXlab platform.
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supercritical anti solvent fractionation of Artemisia Absinthium l conventional extracts tracking artemetin and casticin
Journal of Supercritical Fluids, 2019Co-Authors: Elisa Langa, Azucena Gonzalezcoloma, Juan I Pardo, Carlota Gimenezrota, Maria J Hernaiz, Ana M MainarAbstract:Abstract In the current work the Supercritical Anti-solvent Fractionation (SAF) methodology was applied to conventional extracts from Artemisia Absinthium L. (wormwood). Artemetin and casticin, two compounds found in wormwood extracts and with high structural similarity, were tracked in all the experiments. A Response Surface Methodology (RSM), based on Central Composite Design (CCD) was used for both the experimental design and the results correlation. Studied variables were pressure (from 8.0–15.0 MPa) and CO2 flow rate (from 10 to 60 g·min−1), while temperature (40 °C) and feed solution flow rate (0.45 mL·min−1) were maintained constant. Overall achieved yields were around 70%, being downstream vessel yields much higher than those in the precipitation vessel. The conditions predicted to reach an optimal overall yield and fractionation were 80 MPa and 10 g·min−1 (composite desirability = 0.7443). Casticin and artemetin were mainly obtained in the downstream vessel, being this fraction more enriched in artemetin than casticin.
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comparative chemistry and insect antifeedant action of traditional clevenger and soxhlet and supercritical extracts co2 of two cultivated wormwood Artemisia Absinthium l populations
Industrial Crops and Products, 2011Co-Authors: L Martin, Jesus Burillo, Luis F Julio, Jesus Sanz, Ana M Mainar, Azucena GonzalezcolomaAbstract:Abstract A comparison between traditional extraction techniques (hydrodistillation and organic solvent extraction) and supercritical fluid extraction was made for two different populations and crops of Artemisia Absinthium L., cultivated in the field and aeroponically. The composition of the extracts, volatile and non volatile oils, was analyzed by GC–MS and HPLC–DAD, respectively. The antifeedant and phytotoxic activity of the extracts was tested on insect pests ( Spodoptera littoralis , Myzus persicae and Rhopalosiphum padi ) and plants ( Lactuca sativa and Lolium perenne ). The supercritical extracts exhibited stronger antifeedant effects than the traditional ones (up to 8 times more active) with moderate selective phytotoxic effects on L. perenne root growth (
