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Artemisia Absinthium

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Azucena Gonzalezcoloma – 1st expert on this subject based on the ideXlab platform

  • supercritical anti solvent fractionation of Artemisia Absinthium l conventional extracts tracking artemetin and casticin
    Journal of Supercritical Fluids, 2019
    Co-Authors: Elisa Langa, Azucena Gonzalezcoloma, Juan I Pardo, Carlota Gimenezrota, Maria J Hernaiz, Ana M Mainar


    Abstract In the current work the Supercritical Anti-solvent Fractionation (SAF) methodology was applied to conventional extracts from Artemisia Absinthium L. (wormwood). Artemetin and casticin, two compounds found in wormwood extracts and with high structural similarity, were tracked in all the experiments. A Response Surface Methodology (RSM), based on Central Composite Design (CCD) was used for both the experimental design and the results correlation. Studied variables were pressure (from 8.0–15.0 MPa) and CO2 flow rate (from 10 to 60 g·min−1), while temperature (40 °C) and feed solution flow rate (0.45 mL·min−1) were maintained constant. Overall achieved yields were around 70%, being downstream vessel yields much higher than those in the precipitation vessel. The conditions predicted to reach an optimal overall yield and fractionation were 80 MPa and 10 g·min−1 (composite desirability = 0.7443). Casticin and artemetin were mainly obtained in the downstream vessel, being this fraction more enriched in artemetin than casticin.

  • absolute configuration of the ocimene monoterpenoids from Artemisia Absinthium
    Chirality, 2017
    Co-Authors: Luis F Julio, Azucena Gonzalezcoloma, Carmen E Diaz, Eleuterio Burguenotapia, Nury Perezhernandez, Pedro Josephnathan


    : The absolute configuration (AC) of the naturally occurring ocimenes (-)-(3S,5Z)-2,6-dimethyl-2,3-epoxyocta-5,7-diene (1) and (-)-(3S,5Z)-2,6-dimethylocta-5,7-dien-2,3-diol (2), isolated from the essential oils of domesticated specimens of Artemisia Absinthium, followed by vibrational circular dichroism (VCD) studies of 1, as well as from the acetonide 3 and the monoacetate 4, both derived from 2, since secondary alcohols are not the best functional groups to be present during VCD studies in solution due to intermolecular associations. The AC follows from comparison of experimental and calculated VCD spectra that were obtained by Density Functional Theory computation at the B3LYP/DGDZVP level of theory. Careful nuclear magnetic resonance (NMR) measurements were compared with literature values, providing for the first time systematic 1 H and 13 C chemical shift data. Regarding homonuclear 1 H coupling constants, after performing a few irradiation experiments that showed the presence of several small long-range interactions, the complete set of coupling constants for 3, which is representative of the four studied molecules, was determined by iterations using the PERCH software. This procedure even allowed assigning the pro-R and pro-S methyl group signals of the two gem-dimethyl groups present in 3.

  • chemical and biocidal characterization of two cultivated Artemisia Absinthium populations with different domestication levels
    Industrial Crops and Products, 2015
    Co-Authors: Luis F Julio, Jesus Burillo, Carmen E Diaz, Raimundo Cabrera, Cristina Gimenez, Jesus Sanz, Azucena Gonzalezcoloma


    Abstract The objective of this study was the characterization and valorization of the essential oil from a domesticated Artemisia Absinthium population (Teruel, Spain), and its comparison with another one (Sierra Nevada, Spain) undergoing the domestication process. These populations are being experimentally cultivated in the same field since 2008. We studied their biomass and essential oil production (Clevenger hydrodistillation, HD and semi-industrial vapor-pressure, VP). The domesticated population showed lower chemical variation and higher biomass and essential oil yields, allowing for the registration of a new plant variety. The observed variations in oil composition (HD, VP) between the two populations were mostly quantitative. The oils were characterized by the presence of cis-epoxyocimene, (−)-cis-chrysanthenol, chrysanthenyl acetate, linalool and trans-caryophyllene. The insect antifeedant (Leptinotarsa decemlineata, Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi) and antifungal (Fusarium spp. and Botrytis cinerea) effects of their oils were also tested. All VP extracts showed strong antifungal effects and the active antifungal compounds have been identified.

Peter G Waterman – 2nd expert on this subject based on the ideXlab platform

  • volatile oils from normal and transformed root of Artemisia Absinthium
    Phytochemistry, 1993
    Co-Authors: Alan I Kennedy, S G Deans, Katerina P Svoboda, Alexander I Gray, Peter G Waterman


    Abstract The volatile oil distilled from the roots of field-grown Artemisia Absinthium and from fermenter grown hairy roots of A. Absinthium transformed

R Magherini – 3rd expert on this subject based on the ideXlab platform

  • agrobacterium mediated transformation of Artemisia Absinthium l wormwood and production of secondary metabolites
    Plant Cell Reports, 1997
    Co-Authors: Andrea Bennici, S Schiff, G Roselli, D Mariotti, R Magherini


    Hairy roots were obtained after infection of Artemisia Absinthium shoots with Agrobacterium rhizogenes strains 1855 and LBA 9402. The susceptibility to hairy root transformation varied between plant genotypes and bacterial strains. Hairy roots showed macroscopic differences from control root cultures. Southern blot hybridization confirmed the integration of T-DNA from both p1855 and pBin19, while polymerase chain reaction analysis indicated the presence of the neomycin phosphotransferase gene in the hairy root genome. Subcultured transformed root lines grew well in selective B5 agar-solidified medium containing kanamycin or rifampicin and without hormones. Shake-flask experiments with fast-growing root lines showed that 40 g l–1 was the best sucrose concentration for biomass production, yielding a 463-fold increase in dry weight after 28 days of culture. Great differences were found in the profiles of the essential oils isolated from normal and hairy roots. Gas chromatography/mass spectrometry analysis showed the oil produced by transformed cultures to be a mixture of 50 compounds with only one major component representing 37% of the oil content.