The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Xiao-dong Zheng - One of the best experts on this subject based on the ideXlab platform.
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The Mechanism of Action of Pterostilbene in Xinjiang Wine Grape Against the Growth of Geotrichum citri-aurantii
Food Biotechnology, 2016Co-Authors: Qingjun Kong, Xiao-dong Zheng, Lidan Tu, Zhidong WangAbstract:ABSTRACTPterostilbene controlled the growth of Geotrichum citri-aurantii effectively through experiments of Arthroconidium germination on agar plate, which displayed a strong antifungal activity. Observations using light microscope, scanning electron showed that the morphology and inner structure of the G. citri-aurantii treated with the pterostilbene changed notably. Some of the cells were abnormal or lysed. In order to further clarify the molecular mechanism of action, we examined the binding properties exerted by the pterostilbene on DNA and RNA of G. citri-aurantii fungal cells. Results showed that DNA and RNA of the G. citri-aurantii fungal cells were degraded by the pterostilbene. Moreover, three differentially expressed apoptosis genes of fungi were further examined by semi-quantitative RT-PCR. The results indicated that pterostilbene could cause the expression of up-regulated genes involved in RLM1, RTG1, and RTG3, of which the former two genes were significantly up-regulated.
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In vitro and in vivo antifungal activity of a water‐dilutable cassia oil microemulsion against Geotrichum citri‐aurantii
Journal of the Science of Food and Agriculture, 2012Co-Authors: Shi-Xiang Xu, Yi Chen Li, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed. RESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii. CONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases. Copyright © 2012 Society of Chemical Industry
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In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii.
Journal of the science of food and agriculture, 2012Co-Authors: Shi-Xiang Xu, Li-juan Mao, Yi Chen Li, Xia Liu, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed.\n\nRESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii.\n\nCONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases.
Shi-Xiang Xu - One of the best experts on this subject based on the ideXlab platform.
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In vitro and in vivo antifungal activity of a water‐dilutable cassia oil microemulsion against Geotrichum citri‐aurantii
Journal of the Science of Food and Agriculture, 2012Co-Authors: Shi-Xiang Xu, Yi Chen Li, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed. RESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii. CONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases. Copyright © 2012 Society of Chemical Industry
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In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii.
Journal of the science of food and agriculture, 2012Co-Authors: Shi-Xiang Xu, Li-juan Mao, Yi Chen Li, Xia Liu, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed.\n\nRESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii.\n\nCONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases.
Hui Zhang - One of the best experts on this subject based on the ideXlab platform.
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In vitro and in vivo antifungal activity of a water‐dilutable cassia oil microemulsion against Geotrichum citri‐aurantii
Journal of the Science of Food and Agriculture, 2012Co-Authors: Shi-Xiang Xu, Yi Chen Li, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed. RESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii. CONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases. Copyright © 2012 Society of Chemical Industry
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In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii.
Journal of the science of food and agriculture, 2012Co-Authors: Shi-Xiang Xu, Li-juan Mao, Yi Chen Li, Xia Liu, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed.\n\nRESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii.\n\nCONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases.
Yi Chen Li - One of the best experts on this subject based on the ideXlab platform.
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In vitro and in vivo antifungal activity of a water‐dilutable cassia oil microemulsion against Geotrichum citri‐aurantii
Journal of the Science of Food and Agriculture, 2012Co-Authors: Shi-Xiang Xu, Yi Chen Li, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed. RESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii. CONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases. Copyright © 2012 Society of Chemical Industry
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In vitro and in vivo antifungal activity of a water-dilutable cassia oil microemulsion against Geotrichum citri-aurantii.
Journal of the science of food and agriculture, 2012Co-Authors: Shi-Xiang Xu, Li-juan Mao, Yi Chen Li, Xia Liu, Hui Zhang, Xiao-dong ZhengAbstract:BACKGROUND: Recently, food-grade microemulsions have been of increasing interest to researchers and have shown great potential in industrial applications. In this study a food-grade water-dilutable microemulsion system with cassia oil as oil, ethanol as cosurfactant, Tween 20 as surfactant and water was developed and its antifungal activity in vitro and in vivo against Geotrichum citri-aurantii was assessed.\n\nRESULTS: The phase diagram results confirmed the feasibility of forming a water-dilutable microemulsion based on cassia oil. One microemulsion formulation, cassia oil/ethanol/Tween 20 = 1:3:6 (w/w/w), was selected with the capability to undergo full dilution with water. The average particle size was 6.3 nm. The in vitro antifungal experiments showed that the microemulsion inhibited fungal growth on solid medium and prevented Arthroconidium germination in liquid medium and that cassia oil had stronger activity when encapsulated in the microemulsion. The in vivo antifungal experiments indicated that the water-dilutable microemulsion was effective in preventing postharvest diseases of citrus fruits caused by G. citri-aurantii.\n\nCONCLUSION: The results of this study suggest a promising utilisation of water-dilutable microemulsions based on essential oils for the control of postharvest diseases.
Richard C. Barton - One of the best experts on this subject based on the ideXlab platform.
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Arthroconidia production in Trichophyton rubrum and a new ex vivo model of onychomycosis.
Journal of medical microbiology, 2020Co-Authors: S. Amir Yazdanparast, Richard C. BartonAbstract:The dermatophyte fungus Trichophyton rubrum often produces arthroconidia in vivo, and these cells are thought to be involved in pathogenesis, and, in shed skin scales, to act as a source of infection. The purpose of this study was (i) to examine the environmental and iatrogenic factors which affect arthroconidiation in T. rubrum in vitro, (ii) to look at arthroconidia formation in a large number of clinical isolates of T. rubrum and (iii) to develop a new model for the study of arthroconidia formation in nail tissue. Arthroconidia production was studied in T. rubrum grown on a number of media and under conditions of varying pH, temperature and CO(2) concentration. The effect of the presence of antifungals and steroids on arthroconidia formation was also examined. Nail powder from the healthy toenails of volunteers was used as a substrate for arthroconidial production. On Sabouraud dextrose agar in the presence of 10 % CO(2) plus air, arthroconidial formation occurred optimally at 37 degrees C and pH 7.5, and was maximal at 10 days. Most isolates of T. rubrum showed a similar level of arthroconidial production, and only two out of 50 strains were unable to produce arthroconidia. Subinhibitory levels of some antifungals and betamethasone resulted in the stimulation of arthroconidia formation. Arthroconidial production in ground nail material also occurred under the same optimal conditions, but took longer to reach maximal levels (14 days). These in vitro and ex vivo results provide a useful basis for the understanding of Arthroconidium formation in vivo in infected tissues such as nails.
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Arthroconidia production in Trichophyton rubrum and a new ex vivo model of onychomycosis
Journal of Medical Microbiology, 2006Co-Authors: S. Amir Yazdanparast, Richard C. BartonAbstract:The dermatophyte fungus Trichophyton rubrum often produces arthroconidia in vivo, and these cells are thought to be involved in pathogenesis, and, in shed skin scales, to act as a source of infection. The purpose of this study was (i) to examine the environmental and iatrogenic factors which affect arthroconidiation in T. rubrum in vitro, (ii) to look at arthroconidia formation in a large number of clinical isolates of T. rubrum and (iii) to develop a new model for the study of arthroconidia formation in nail tissue. Arthroconidia production was studied in T. rubrum grown on a number of media and under conditions of varying pH, temperature and CO2 concentration. The effect of the presence of antifungals and steroids on arthroconidia formation was also examined. Nail powder from the healthy toenails of volunteers was used as a substrate for arthroconidial production. On Sabouraud dextrose agar in the presence of 10 % CO2 plus air, arthroconidial formation occurred optimally at 37 °C and pH 7.5, and was maximal at 10 days. Most isolates of T. rubrum showed a similar level of arthroconidial production, and only two out of 50 strains were unable to produce arthroconidia. Subinhibitory levels of some antifungals and betamethasone resulted in the stimulation of arthroconidia formation. Arthroconidial production in ground nail material also occurred under the same optimal conditions, but took longer to reach maximal levels (14 days). These in vitro and ex vivo results provide a useful basis for the understanding of Arthroconidium formation in vivo in infected tissues such as nails.
