The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Isabel Borges De Kestelman - One of the best experts on this subject based on the ideXlab platform.
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Arthroderma gypseum y su anamorfo en el jardin central de la universidad nacional de tucuman argentina
Boletín Micológico, 2007Co-Authors: Aida Van Gelderen, Isabel Borges De KestelmanAbstract:Con el objeto de contribuir al conocimiento de la micobiota en areas publicas, en especial en la busqueda de propagulos infectivos para el hombre y los animales de dermatofitos geofilicos desde sus fuentes saprofiticas, se estudiaron en el lapso de 2 anos, 8 muestras superficiales de suelo (una cada 3 meses) desde los jardines centrales de la Universidad Nacional de Tucuman mediante la Tecnica de Vanbreuseghem, usando pelos de ninos como anzuelo queratinico. Las 8 muestras sembradas en 24 placas de Petri, presentaron todas al cabo de 25-30 dias la presencia de Arthroderma gypseum y su anamorfo Microsporum gypseum como unico dermatofito presente. Los cultivos en Sabouraud glucosado y en Sabouraud al 10% (medio de Takashio), permitieron identificar las cepas tipicas del anamorfo. La presencia y prevalencia en el tiempo de este agente en las muestras colectadas en dependencias universitarias rodeadas de aulas, laboratorios y oficinas, posee un impacto en salud publica y ambiental por su conocido oportunismo en pacientes inmunocompetentes e inmunocomprometidos.
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Arthroderma gypseum Y SU ANAMORFO EN EL JARDIN CENTRAL DE LA UNIVERSIDAD NACIONAL DE TUCUMAN, ARGENTINA. (Artroderma gypseum and its anamorph from the central garden of the Universidad Nacional deTucuman, Argentina)
2007Co-Authors: Aida Van Gelderen De Komaid, Isabel Borges De KestelmanAbstract:With the purpose of knowing the presence of mycobiota in public areas, mainly in the search of propagules of geophilic dermatophytes which are infective for man and animals from their saprophytic sources, 8 superficial samples of soil (every 3 month), collected from the central gardens of the Universidad Nacional de Tucuman were studied during a 2-year period, by using the Vanbreuseghem Technique which employs children hair as a keratinic bait. The overall 8 samples sowed on 24 Petri plates revealed after 25-30 days the presence of Arthroderma gypseum and its anamorph Microsporum gypseum as the only dermatophyte present. Cultures in glucose Sabouraud and in 10% Sabouraud (Takashio medium), allowed the identification of the typical strains of the anamorph. Presence and prevalence in time of this agent in samples collected from university buildings surrounded by classrooms, laboratories and offices means an impact on public and environmental health due to its well known opportunism in immunocompetent and immunocompromised patients.
Atsuhiko Hasegawa - One of the best experts on this subject based on the ideXlab platform.
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Urease gene of Trichophyton rubrum var. raubitschekii.
The Journal of dermatology, 2012Co-Authors: Rui Kano, Atsuhiko Hasegawa, Takashi Sugita, Takashi Mochizuki, M. HirumaAbstract:The recent description (in GenBank) of a urease-encoding gene from Trichophyton rubrum provides an opportunity to compare this species to the highly similar T. rubrum var. raubitschekii. Therefore, the corresponding genomic DNA was recovered from T. rubrum var. raubitschekii, and the sequence and expression were compared for this urease gene in urease-positive and -negative isolates of T. rubrum and T. rubrum var. raubitschekii. The sequence (2371 bp) of the T. rubrum var. raubitschekii urease gene revealed the presence of three exons. Except for a three-amino acid insertion, the predicted proteins were identical, but demonstrated protein identity of approximately 70% compared to an Arthroderma gypseum homolog. Reverse transcription polymerase chain reaction analysis revealed the presence of a corresponding transcript in both urease-positive T. rubrum var. raubitschekii isolates examined; the same analysis did not detect this transcript in urease-negative isolates of T. rubrum.
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Metalloprotease gene of Arthroderma gypseum.
Japanese journal of infectious diseases, 2005Co-Authors: Rui Kano, Tsuyoshi Yamada, Koichi Makimura, Hideyo Yamaguchi, Shinichi Watanabe, Atsuhiko HasegawaAbstract:The full-length cDNA sequence for metalloprotease (MEP) of Arthroderma gypseum (one of the teleomorphs of the Microsporum gypseum complex) was determined by the 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE methods using cDNA as a template. The full-length cDNA sequence of the MEP (2,670 bp) gene was proved to encode 677 amino acids. The amino acid sequence of the A. gypseum MEP gene shared about 89 and 66% sequence similarity with the conserved region of the Microsporum canis MEP gene and Aspergillus fumigatus, respectively. Southern hybridization analysis of genomic DNA with an MEP probe gave many distinct bands in BamHI, EcoRI and HindIII digests of genomic DNA from A. gypseum. Reverse transcriptase-PCR analysis suggested that keratin might stimulate the expression of MEP mRNA in A. gypseum.
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Microsporum gypseum isolated from a feline case of dermatophytosis.
Mycoses, 2001Co-Authors: Rui Kano, Kazuo Yasuda, Yuka Nakamura, Atsuhiko HasegawaAbstract:The 1- to 2-month-old female cross-breed cat presented with alopecia, erythema and many crusts were present on the tail. Microscopic examination of crusts from the tail disclosed epithelial debris, exudate, mycelium, and arthrospores. Microsporum gypseum which is rarely isolated from cats as a causative agent of dermatophytsis was cultured from the crusts on a 1/10 Sabouraud glucose agar at 27 degrees C for 1 week. The isolate of M. gypseum from the cat was examined by random amplification of polymorphic DNA (RAPD), chitin synthase 1 gene (CHS1) sequence and mating experiments. The RAPD band patterns of the clinical isolate of M. gypseum was identical to those of tester strains of Arthroderma gypseum. Nucleotide sequence analysis of the CHS1 gene fragments from the isolate and a tester strain of A. gypseum showed 100% similarity. The mating experiments on the clinical isolate of M. gypseum completely agreed with the results from RAPD and CHS1 gene sequence. The isolate from the cat was confirmed to be A. gypseum (-) mating type, which was consistent with the result of mycological examination by molecular analyses.
Aida Van Gelderen - One of the best experts on this subject based on the ideXlab platform.
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Arthroderma gypseum y su anamorfo en el jardin central de la universidad nacional de tucuman argentina
Boletín Micológico, 2007Co-Authors: Aida Van Gelderen, Isabel Borges De KestelmanAbstract:Con el objeto de contribuir al conocimiento de la micobiota en areas publicas, en especial en la busqueda de propagulos infectivos para el hombre y los animales de dermatofitos geofilicos desde sus fuentes saprofiticas, se estudiaron en el lapso de 2 anos, 8 muestras superficiales de suelo (una cada 3 meses) desde los jardines centrales de la Universidad Nacional de Tucuman mediante la Tecnica de Vanbreuseghem, usando pelos de ninos como anzuelo queratinico. Las 8 muestras sembradas en 24 placas de Petri, presentaron todas al cabo de 25-30 dias la presencia de Arthroderma gypseum y su anamorfo Microsporum gypseum como unico dermatofito presente. Los cultivos en Sabouraud glucosado y en Sabouraud al 10% (medio de Takashio), permitieron identificar las cepas tipicas del anamorfo. La presencia y prevalencia en el tiempo de este agente en las muestras colectadas en dependencias universitarias rodeadas de aulas, laboratorios y oficinas, posee un impacto en salud publica y ambiental por su conocido oportunismo en pacientes inmunocompetentes e inmunocomprometidos.
Rui Kano - One of the best experts on this subject based on the ideXlab platform.
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Urease gene of Trichophyton rubrum var. raubitschekii.
The Journal of dermatology, 2012Co-Authors: Rui Kano, Atsuhiko Hasegawa, Takashi Sugita, Takashi Mochizuki, M. HirumaAbstract:The recent description (in GenBank) of a urease-encoding gene from Trichophyton rubrum provides an opportunity to compare this species to the highly similar T. rubrum var. raubitschekii. Therefore, the corresponding genomic DNA was recovered from T. rubrum var. raubitschekii, and the sequence and expression were compared for this urease gene in urease-positive and -negative isolates of T. rubrum and T. rubrum var. raubitschekii. The sequence (2371 bp) of the T. rubrum var. raubitschekii urease gene revealed the presence of three exons. Except for a three-amino acid insertion, the predicted proteins were identical, but demonstrated protein identity of approximately 70% compared to an Arthroderma gypseum homolog. Reverse transcription polymerase chain reaction analysis revealed the presence of a corresponding transcript in both urease-positive T. rubrum var. raubitschekii isolates examined; the same analysis did not detect this transcript in urease-negative isolates of T. rubrum.
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Metalloprotease gene of Arthroderma gypseum.
Japanese journal of infectious diseases, 2005Co-Authors: Rui Kano, Tsuyoshi Yamada, Koichi Makimura, Hideyo Yamaguchi, Shinichi Watanabe, Atsuhiko HasegawaAbstract:The full-length cDNA sequence for metalloprotease (MEP) of Arthroderma gypseum (one of the teleomorphs of the Microsporum gypseum complex) was determined by the 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE methods using cDNA as a template. The full-length cDNA sequence of the MEP (2,670 bp) gene was proved to encode 677 amino acids. The amino acid sequence of the A. gypseum MEP gene shared about 89 and 66% sequence similarity with the conserved region of the Microsporum canis MEP gene and Aspergillus fumigatus, respectively. Southern hybridization analysis of genomic DNA with an MEP probe gave many distinct bands in BamHI, EcoRI and HindIII digests of genomic DNA from A. gypseum. Reverse transcriptase-PCR analysis suggested that keratin might stimulate the expression of MEP mRNA in A. gypseum.
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Microsporum gypseum isolated from a feline case of dermatophytosis.
Mycoses, 2001Co-Authors: Rui Kano, Kazuo Yasuda, Yuka Nakamura, Atsuhiko HasegawaAbstract:The 1- to 2-month-old female cross-breed cat presented with alopecia, erythema and many crusts were present on the tail. Microscopic examination of crusts from the tail disclosed epithelial debris, exudate, mycelium, and arthrospores. Microsporum gypseum which is rarely isolated from cats as a causative agent of dermatophytsis was cultured from the crusts on a 1/10 Sabouraud glucose agar at 27 degrees C for 1 week. The isolate of M. gypseum from the cat was examined by random amplification of polymorphic DNA (RAPD), chitin synthase 1 gene (CHS1) sequence and mating experiments. The RAPD band patterns of the clinical isolate of M. gypseum was identical to those of tester strains of Arthroderma gypseum. Nucleotide sequence analysis of the CHS1 gene fragments from the isolate and a tester strain of A. gypseum showed 100% similarity. The mating experiments on the clinical isolate of M. gypseum completely agreed with the results from RAPD and CHS1 gene sequence. The isolate from the cat was confirmed to be A. gypseum (-) mating type, which was consistent with the result of mycological examination by molecular analyses.
Aida Van Gelderen De Komaid - One of the best experts on this subject based on the ideXlab platform.
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Arthroderma gypseum Y SU ANAMORFO EN EL JARDIN CENTRAL DE LA UNIVERSIDAD NACIONAL DE TUCUMAN, ARGENTINA. (Artroderma gypseum and its anamorph from the central garden of the Universidad Nacional deTucuman, Argentina)
2007Co-Authors: Aida Van Gelderen De Komaid, Isabel Borges De KestelmanAbstract:With the purpose of knowing the presence of mycobiota in public areas, mainly in the search of propagules of geophilic dermatophytes which are infective for man and animals from their saprophytic sources, 8 superficial samples of soil (every 3 month), collected from the central gardens of the Universidad Nacional de Tucuman were studied during a 2-year period, by using the Vanbreuseghem Technique which employs children hair as a keratinic bait. The overall 8 samples sowed on 24 Petri plates revealed after 25-30 days the presence of Arthroderma gypseum and its anamorph Microsporum gypseum as the only dermatophyte present. Cultures in glucose Sabouraud and in 10% Sabouraud (Takashio medium), allowed the identification of the typical strains of the anamorph. Presence and prevalence in time of this agent in samples collected from university buildings surrounded by classrooms, laboratories and offices means an impact on public and environmental health due to its well known opportunism in immunocompetent and immunocompromised patients.
