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Arthroderma otae
The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Anna Brillowskadąbrowska – 1st expert on this subject based on the ideXlab platform
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pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
Journal of The European Academy of Dermatology and Venereology, 2016Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna BrillowskadąbrowskaAbstract:Objectives
The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex
Methods
Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.
Result
The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.
Conclusion
We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.
Natalia Kobylak – 2nd expert on this subject based on the ideXlab platform
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pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
Journal of The European Academy of Dermatology and Venereology, 2016Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna BrillowskadąbrowskaAbstract:Objectives
The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex
Methods
Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.
Result
The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.
Conclusion
We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.
Ewelina Kurzyk – 3rd expert on this subject based on the ideXlab platform
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pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
Journal of The European Academy of Dermatology and Venereology, 2016Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna BrillowskadąbrowskaAbstract:Objectives
The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex
Methods
Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.
Result
The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.
Conclusion
We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.