Arthroderma otae - Explore the Science & Experts | ideXlab

Scan Science and Technology

Contact Leading Edge Experts & Companies

Arthroderma otae

The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform

Anna Brillowskadąbrowska – 1st expert on this subject based on the ideXlab platform

  • pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
    Journal of The European Academy of Dermatology and Venereology, 2016
    Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna Brillowskadąbrowska

    Abstract:

    Objectives

    The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex

    Methods

    Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.

    Result

    The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.

    Conclusion

    We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.

Natalia Kobylak – 2nd expert on this subject based on the ideXlab platform

  • pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
    Journal of The European Academy of Dermatology and Venereology, 2016
    Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna Brillowskadąbrowska

    Abstract:

    Objectives

    The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex

    Methods

    Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.

    Result

    The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.

    Conclusion

    We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.

Ewelina Kurzyk – 3rd expert on this subject based on the ideXlab platform

  • pcr and real time pcr approaches to the identification of Arthroderma otae species microsporum canis and microsporum audouinii microsporum ferrugineum
    Journal of The European Academy of Dermatology and Venereology, 2016
    Co-Authors: Natalia Kobylak, Barbara Bykowska, Ewelina Kurzyk, Roman Nowicki, Anna Brillowskadąbrowska

    Abstract:

    Objectives

    The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex

    Methods

    Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture.

    Result

    The two assays presented in this study enable the identification of M. canis and M. audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR.

    Conclusion

    We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. audouinii/M. ferrugineum DNA from a pure culture.