Artificial Feeding

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Frans Jongejan - One of the best experts on this subject based on the ideXlab platform.

Emanuela Olivieri - One of the best experts on this subject based on the ideXlab platform.

Abdu F Azad - One of the best experts on this subject based on the ideXlab platform.

  • infection and transovarial transmission of rickettsiae in dermacentor variabilis ticks acquired by Artificial Feeding
    Vector-borne and Zoonotic Diseases, 2001
    Co-Authors: Kevin R Macaluso, Daniel E Sonenshine, Shane M Ceraul, Abdu F Azad
    Abstract:

    In this study we examined the efficiency of an in vitro Feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary Feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 and 6.77 µl. However, ingestion of fluid was not correlated to weight gain during capillary Feeding. Uninfected and partially fed laboratory-reared female Dermacentor variabilis ticks were exposed to either Rickettsia montana- or Rickettsia rhipicephali-infected Vero cells via microcapillary tubes, returned to rabbit hosts, and allowed to feed to repletion. All tissues collected from ticks allowed to feed overnight on rickettsiae-infected fluids were found to be infected when examined by IFA. When rickettsiae-infected and uninfected capillary-fed ticks were allowed to feed to repletion and lay eggs, no significant differences in mean engorgem...

Ard M Nijhof - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of a semi-automated in vitro Feeding system for Dermacentor reticulatus and Ixodes ricinus adults
    Parasitology Research, 2018
    Co-Authors: Bettina Bohme, Christoph Krull, Peter-henning Clausen, Ard M Nijhof
    Abstract:

    The long Feeding duration of ixodid ticks and need for regular blood changes turns the Artificial Feeding of ticks into a tedious process. To reduce the number of blood changes, a semi-automated system (SAS) for the Artificial Feeding of hard ticks was developed and evaluated. It consisted of a glass Feeding reservoir that can accommodate six tick Feeding chambers. A peristaltic pump was used to pump blood through the Feeding reservoir, which was changed once daily. Groups of Dermacentor reticulatus and Ixodes ricinus adults were fed simultaneously in both the SAS and a conventional in vitro Feeding system. In the conventional system, Feeding chambers were hung inside a glass beaker filled with blood that was replaced twice daily. Dermacentor reticulatus adults fed in the SAS obtained significantly higher engorgement weights. Although engorgement rates between both systems were comparable, significantly more SAS-fed females laid fertile egg batches. The egg batch weight of SAS-fed females was also significantly higher. In contrast, the engorgement rate and fecundity of SAS-fed I. ricinus were significantly reduced in comparison to ticks fed in the conventional system. This reduction was likely to be caused by fungal infestation, which could spread between Feeding chambers in the SAS. Although the SAS reduced the workload compared to the conventional Feeding system and showed promising results for the in vitro Feeding of D. reticulatus adults, measures to prevent fungal infestations in the SAS should be considered in future studies.

Kevin R Macaluso - One of the best experts on this subject based on the ideXlab platform.

  • infection and transovarial transmission of rickettsiae in dermacentor variabilis ticks acquired by Artificial Feeding
    Vector-borne and Zoonotic Diseases, 2001
    Co-Authors: Kevin R Macaluso, Daniel E Sonenshine, Shane M Ceraul, Abdu F Azad
    Abstract:

    In this study we examined the efficiency of an in vitro Feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary Feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 and 6.77 µl. However, ingestion of fluid was not correlated to weight gain during capillary Feeding. Uninfected and partially fed laboratory-reared female Dermacentor variabilis ticks were exposed to either Rickettsia montana- or Rickettsia rhipicephali-infected Vero cells via microcapillary tubes, returned to rabbit hosts, and allowed to feed to repletion. All tissues collected from ticks allowed to feed overnight on rickettsiae-infected fluids were found to be infected when examined by IFA. When rickettsiae-infected and uninfected capillary-fed ticks were allowed to feed to repletion and lay eggs, no significant differences in mean engorgem...