The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
Ngadiman N. H. A. - One of the best experts on this subject based on the ideXlab platform.
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3D biofabrication of thermoplastic polyurethane (TPU)/poly-L-lactic acid (PLLA) electrospun nanofibers containing maghemite (γ-Fe2O3) for tissue engineering aortic heart valve
'MDPI AG', 2017Co-Authors: Fallahiarezoudar E., Ahmadipourroudposht M., Yusof N. M., Idris A., Ngadiman N. H. A.Abstract:Valvular dysfunction as the prominent reason of heart failure may causes morbidity and mortality around the world. The inability of human body to regenerate the defected heart valves necessitates the development of the Artificial Prosthesis to be replaced. Besides, the lack of capacity to grow, repair or remodel of an Artificial valves and biological difficulty such as infection or inflammation make the development of tissue engineering heart valve (TEHV) concept. This research presented the use of compound of poly-L-lactic acid (PLLA), thermoplastic polyurethane (TPU) and maghemite nanoparticle ( -Fe2O3) as the potential biomaterials to develop three-dimensional (3D) aortic heart valve scaffold. Electrospinning was used for fabricating the 3D scaffold. The steepest ascent followed by the response surface methodology was used to optimize the electrospinning parameters involved in terms of elastic modulus. The structural and porosity properties of fabricated scaffold were characterized using FE-SEM and liquid displacement technique, respectively. The 3D scaffold was then seeded with aortic smooth muscle cells (AOSMCs) and biological behavior in terms of cell attachment and proliferation during 34 days of incubation was characterized using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal laser microscopy. Furthermore, the mechanical properties in terms of elastic modulus and stiffness were investigated after cell seeding through macro-indentation test. The analysis indicated the formation of ultrafine quality of nanofibers with diameter distribution of 178 ± 45 nm and 90.72% porosity. In terms of cell proliferation, the results exhibited desirable proliferation (109.32 ± 3.22% compared to the control) of cells over the 3D scaffold in 34 days of incubation. The elastic modulus and stiffness index after cell seeding were founded to be 22.78 ± 2.12 MPa and 1490.9 ± 12 Nmm2, respectively. Overall, the fabricated 3D scaffold exhibits desirable structural, biological and mechanical properties and has the potential to be used in vivo
Ngadiman, Nor Hasrul Akhmal - One of the best experts on this subject based on the ideXlab platform.
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3D Biofabrication of Thermoplastic Polyurethane (TPU)/Poly-l-lactic Acid (PLLA) Electrospun Nanofibers Containing Maghemite (-Fe2O3) for Tissue Engineering Aortic Heart Valve
'MDPI AG', 2017Co-Authors: Fallahiarezoudar Ehsan, Ahmadipourroudposht Mohaddeseh, Yusof, Noordin Mohd., Idris Ani, Ngadiman, Nor Hasrul AkhmalAbstract:Valvular dysfunction as the prominent reason of heart failure may causes morbidity and mortality around the world. The inability of human body to regenerate the defected heart valves necessitates the development of the Artificial Prosthesis to be replaced. Besides, the lack of capacity to grow, repair or remodel of an Artificial valves and biological difficulty such as infection or inflammation make the development of tissue engineering heart valve (TEHV) concept. This research presented the use of compound of poly-l-lactic acid (PLLA), thermoplastic polyurethane (TPU) and maghemite nanoparticle (-Fe2O3) as the potential biomaterials to develop three-dimensional (3D) aortic heart valve scaffold. Electrospinning was used for fabricating the 3D scaffold. The steepest ascent followed by the response surface methodology was used to optimize the electrospinning parameters involved in terms of elastic modulus. The structural and porosity properties of fabricated scaffold were characterized using FE-SEM and liquid displacement technique, respectively. The 3D scaffold was then seeded with aortic smooth muscle cells (AOSMCs) and biological behavior in terms of cell attachment and proliferation during 34 days of incubation was characterized using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal laser microscopy. Furthermore, the mechanical properties in terms of elastic modulus and stiffness were investigated after cell seeding through macro-indentation test. The analysis indicated the formation of ultrafine quality of nanofibers with diameter distribution of 178 +/- 45 nm and 90.72% porosity. In terms of cell proliferation, the results exhibited desirable proliferation (109.32 +/- 3.22% compared to the control) of cells over the 3D scaffold in 34 days of incubation. The elastic modulus and stiffness index after cell seeding were founded to be 22.78 +/- 2.12 MPa and 1490.9 +/- 12 Nmm(2), respectively. Overall, the fabricated 3D scaffold exhibits desirable structural, biological and mechanical properties and has the potential to be used in vivo
Yasuhiko Shimizu - One of the best experts on this subject based on the ideXlab platform.
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the experimental replacement of a cervical esophageal segment with an Artificial Prosthesis with the use of collagen matrix and a silicone stent
The Journal of Thoracic and Cardiovascular Surgery, 1998Co-Authors: Yukinobu Takimoto, Tatsuo Nakamura, Yasumichi Yamamoto, Tetsuya Kiyotani, Masayoshi Teramachi, Yasuhiko ShimizuAbstract:Abstract Objective: Attempts have been made to replace esophageal defects with a variety of Artificial materials. However, because of the Artificial nature of the materials, problems such as infection, leakage, stricture, or dislocation could not be avoided. Therefore we have designed a new type of Artificial esophagus that is gradually replaced by host tissue. Methods: Our Artificial esophagus was a two-layered tube consisting of a collagen sponge matrix and an inner silicone stent. We used it to replace 5 cm esophageal segmental defects in 43 dogs, and the inner silicone stent was removed endoscopically at weekly intervals from 2 to 4 weeks. Results: In the 27 dogs from which the silicone stent was removed at 2 or 3 weeks, constriction of the regenerated esophagus progressed and the dogs became unable to swallow within 6 months. In the 16 dogs from which the silicone stent was removed at 4 weeks, highly regenerated esophageal tissue successfully replaced the defect, leaving no foreign body in the host. Moreover, the regenerated esophagi had stratified flattened epithelia, striated muscle tissue composed of an inner circular and an outer longitudinal muscle layer, and esophageal glands. Conclusions: Even in mature adult higher mammals, esophageal high-order structures can be regenerated provided that an adequate three-dimensional extracellular structure is put in place for a sufficient period. (J Thorac Cardiovasc Surg 1998;116:98-106)
Fallahiarezoudar E. - One of the best experts on this subject based on the ideXlab platform.
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3D biofabrication of thermoplastic polyurethane (TPU)/poly-L-lactic acid (PLLA) electrospun nanofibers containing maghemite (γ-Fe2O3) for tissue engineering aortic heart valve
'MDPI AG', 2017Co-Authors: Fallahiarezoudar E., Ahmadipourroudposht M., Yusof N. M., Idris A., Ngadiman N. H. A.Abstract:Valvular dysfunction as the prominent reason of heart failure may causes morbidity and mortality around the world. The inability of human body to regenerate the defected heart valves necessitates the development of the Artificial Prosthesis to be replaced. Besides, the lack of capacity to grow, repair or remodel of an Artificial valves and biological difficulty such as infection or inflammation make the development of tissue engineering heart valve (TEHV) concept. This research presented the use of compound of poly-L-lactic acid (PLLA), thermoplastic polyurethane (TPU) and maghemite nanoparticle ( -Fe2O3) as the potential biomaterials to develop three-dimensional (3D) aortic heart valve scaffold. Electrospinning was used for fabricating the 3D scaffold. The steepest ascent followed by the response surface methodology was used to optimize the electrospinning parameters involved in terms of elastic modulus. The structural and porosity properties of fabricated scaffold were characterized using FE-SEM and liquid displacement technique, respectively. The 3D scaffold was then seeded with aortic smooth muscle cells (AOSMCs) and biological behavior in terms of cell attachment and proliferation during 34 days of incubation was characterized using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal laser microscopy. Furthermore, the mechanical properties in terms of elastic modulus and stiffness were investigated after cell seeding through macro-indentation test. The analysis indicated the formation of ultrafine quality of nanofibers with diameter distribution of 178 ± 45 nm and 90.72% porosity. In terms of cell proliferation, the results exhibited desirable proliferation (109.32 ± 3.22% compared to the control) of cells over the 3D scaffold in 34 days of incubation. The elastic modulus and stiffness index after cell seeding were founded to be 22.78 ± 2.12 MPa and 1490.9 ± 12 Nmm2, respectively. Overall, the fabricated 3D scaffold exhibits desirable structural, biological and mechanical properties and has the potential to be used in vivo
Fallahiarezoudar Ehsan - One of the best experts on this subject based on the ideXlab platform.
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3D Biofabrication of Thermoplastic Polyurethane (TPU)/Poly-l-lactic Acid (PLLA) Electrospun Nanofibers Containing Maghemite (-Fe2O3) for Tissue Engineering Aortic Heart Valve
'MDPI AG', 2017Co-Authors: Fallahiarezoudar Ehsan, Ahmadipourroudposht Mohaddeseh, Yusof, Noordin Mohd., Idris Ani, Ngadiman, Nor Hasrul AkhmalAbstract:Valvular dysfunction as the prominent reason of heart failure may causes morbidity and mortality around the world. The inability of human body to regenerate the defected heart valves necessitates the development of the Artificial Prosthesis to be replaced. Besides, the lack of capacity to grow, repair or remodel of an Artificial valves and biological difficulty such as infection or inflammation make the development of tissue engineering heart valve (TEHV) concept. This research presented the use of compound of poly-l-lactic acid (PLLA), thermoplastic polyurethane (TPU) and maghemite nanoparticle (-Fe2O3) as the potential biomaterials to develop three-dimensional (3D) aortic heart valve scaffold. Electrospinning was used for fabricating the 3D scaffold. The steepest ascent followed by the response surface methodology was used to optimize the electrospinning parameters involved in terms of elastic modulus. The structural and porosity properties of fabricated scaffold were characterized using FE-SEM and liquid displacement technique, respectively. The 3D scaffold was then seeded with aortic smooth muscle cells (AOSMCs) and biological behavior in terms of cell attachment and proliferation during 34 days of incubation was characterized using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal laser microscopy. Furthermore, the mechanical properties in terms of elastic modulus and stiffness were investigated after cell seeding through macro-indentation test. The analysis indicated the formation of ultrafine quality of nanofibers with diameter distribution of 178 +/- 45 nm and 90.72% porosity. In terms of cell proliferation, the results exhibited desirable proliferation (109.32 +/- 3.22% compared to the control) of cells over the 3D scaffold in 34 days of incubation. The elastic modulus and stiffness index after cell seeding were founded to be 22.78 +/- 2.12 MPa and 1490.9 +/- 12 Nmm(2), respectively. Overall, the fabricated 3D scaffold exhibits desirable structural, biological and mechanical properties and has the potential to be used in vivo
