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Françoise Muller - One of the best experts on this subject based on the ideXlab platform.
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biochemical analysis of Ascites fluid as an aid to etiological diagnosis a series of 100 cases of nonimmune fetal Ascites
Prenatal Diagnosis, 2015Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, V Houfflindebarge, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P < 0.001) low levels of total protein in Ascites of urinary origin, high levels of digestive enzymes in Ascites of digestive origin, and high β2-microglobulin in infectious Ascites. Vacuolated cells were observed in all ten storage metabolic diseases. Conclusion Sampling of fetal Ascites fluid for biochemical and cytological examination provides important additional information. We propose a two-step management: (1) detailed ultrasound scan examination, maternal blood analysis, and fetal karyotyping and (2) biochemical and cytological analyses. On the basis of such an approach, 63% and 96% of etiologies would have been identified in our series after the first and second steps, respectively. © 2014 John Wiley & Sons, Ltd.
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Biochemical analysis of Ascites fluid as an aid to etiological diagnosis: a series of 100 cases of nonimmune fetal Ascites.
Prenatal diagnosis, 2014Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Véronique Houfflin-debarge, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P
Sophie Dreux - One of the best experts on this subject based on the ideXlab platform.
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biochemical analysis of Ascites fluid as an aid to etiological diagnosis a series of 100 cases of nonimmune fetal Ascites
Prenatal Diagnosis, 2015Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, V Houfflindebarge, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P < 0.001) low levels of total protein in Ascites of urinary origin, high levels of digestive enzymes in Ascites of digestive origin, and high β2-microglobulin in infectious Ascites. Vacuolated cells were observed in all ten storage metabolic diseases. Conclusion Sampling of fetal Ascites fluid for biochemical and cytological examination provides important additional information. We propose a two-step management: (1) detailed ultrasound scan examination, maternal blood analysis, and fetal karyotyping and (2) biochemical and cytological analyses. On the basis of such an approach, 63% and 96% of etiologies would have been identified in our series after the first and second steps, respectively. © 2014 John Wiley & Sons, Ltd.
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Biochemical analysis of Ascites fluid as an aid to etiological diagnosis: a series of 100 cases of nonimmune fetal Ascites.
Prenatal diagnosis, 2014Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Véronique Houfflin-debarge, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P
Lei Zhu - One of the best experts on this subject based on the ideXlab platform.
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detection of soluble apo 1 fas in plasma pleural and Ascites fluid of malignant tumor patients and its clinical significance
Journal of Zhejiang University (Medical Sciences), 2003Co-Authors: Luhong Luo, Lei ZhuAbstract:OBJECTIVE To study the changes of soluble Apo-1/Fas levels in plasma, pleural and Ascites fluid of malignant tumor patients and to evaluate their clinical significance. METHODS The soluble Apo-1/Fas levels were measured by enzyme-linked immunosorbent assay (ELISA) in the plasma of 157 malignant tumor patients and 25 normal controls as well as in the pleural and ascite fluids of 129 patients with various diseases. RESULT The plasma soluble Apo-1/Fas levels in acute and chronic leukemia and multiple myeloma were significantly higher than those in normal controls (P <0.05). The plasma soluble Apo-1/Fas levels in chronic myeloid leukemia and chronic lymphocytic leukemia were significantly higher than those in acute myeloid leukemia and acute lymphocytic leukemia, respectively (P <0.05). After chemotherapy, the plasma soluble Apo-1/Fas levels in complete remission group were distinctly decreased(P <0.05),whereas the levels in no remission and recurrence groups remained high. Compared with normal controls, the plasma soluble Apo-1/Fas levels in solid tumors were significantly increased (P <0.01), and the levels in metastasis cancers were significantly higher than those in non-metastasis cancer (P <0.0 1). Simultaneously the levels in remission cancer patients after operation and radiotherapy were distinctly lower than those before treatment(P <0.01), but were significantly increased in recurrence cancer patients (P <0.01). The soluble Apo-1/Fas levels in pleural and Ascites fluid of malignant tumors were significantly higher than those in tuberculous effusions and transudates. CONCLUSION The soluble Apo-1/Fas levels in plasma, pleural and Ascites fluid of malignant tumor patients are markedly increased, which might be associated with the progress of cancers. The changes of soluble Apo-1/Fas levels may be useful for understanding the pathologic process of cancers and to differential diagnosis of various pleural and Ascites fluids.
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Detection of soluble Apo-1/Fas in plasma, pleural and Ascites fluid of malignant tumor patients and its clinical significance
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2003Co-Authors: Luhong Luo, Lei ZhuAbstract:OBJECTIVE To study the changes of soluble Apo-1/Fas levels in plasma, pleural and Ascites fluid of malignant tumor patients and to evaluate their clinical significance. METHODS The soluble Apo-1/Fas levels were measured by enzyme-linked immunosorbent assay (ELISA) in the plasma of 157 malignant tumor patients and 25 normal controls as well as in the pleural and ascite fluids of 129 patients with various diseases. RESULT The plasma soluble Apo-1/Fas levels in acute and chronic leukemia and multiple myeloma were significantly higher than those in normal controls (P
Diane Provencher - One of the best experts on this subject based on the ideXlab platform.
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characterization of ovarian cancer Ascites on cell invasion proliferation spheroid formation gene expression in an in vitro model of epithelial ovarian cancer
Neoplasia, 2007Co-Authors: Marieline Puiffe, Cecile Le Page, Abdelali Filalimouhim, Magdalena Zietarska, Veronique Ouellet, Patricia N Tonin, Mario Chevrette, Diane ProvencherAbstract:At least one third of all cases of epithelial ovarian cancer are associated with the production of Ascites, although its effect on tumor cell microenvironment remains poorly understood. This study addresses the effect of the heterologous acellular fraction of ovarian cancer-derived Ascites on a cell line (OV-90) derived from the chemotherapy-naive ovarian cancer patient. Ascites were assayed for their effect on cell invasion, growth, and spheroid formation. When compared to either no serum or 5% serum, Ascites fell into one of two categories: stimulatory or inhibitory. RNA from OV-90 cells exposed to selected Ascites were arrayed on an Affymetrix HG-U133A GeneChip. A supervised analysis identified a number of differentially expressed genes and quantitative polymerase chain reaction validation based on OV-90 cells exposed to 54 independent Ascites demonstrated that stimulatory Ascites affected the expression of ISGF3G, TRIB1, MKP1, RGS4, PLEC1, and MOSPD1 genes. In addition, TRIB1 expression was shown to independently correlate with prognosis when its expression was ascertained in an independent set of primary cultures established from ovarian Ascites. The data support the validity of the strategy to uncover molecular events that are associated with tumor cell behavior and highlight the impact of Ascites on the cellular and molecular parameters of ovarian cancer.
Honorine Fenaux - One of the best experts on this subject based on the ideXlab platform.
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biochemical analysis of Ascites fluid as an aid to etiological diagnosis a series of 100 cases of nonimmune fetal Ascites
Prenatal Diagnosis, 2015Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, V Houfflindebarge, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P < 0.001) low levels of total protein in Ascites of urinary origin, high levels of digestive enzymes in Ascites of digestive origin, and high β2-microglobulin in infectious Ascites. Vacuolated cells were observed in all ten storage metabolic diseases. Conclusion Sampling of fetal Ascites fluid for biochemical and cytological examination provides important additional information. We propose a two-step management: (1) detailed ultrasound scan examination, maternal blood analysis, and fetal karyotyping and (2) biochemical and cytological analyses. On the basis of such an approach, 63% and 96% of etiologies would have been identified in our series after the first and second steps, respectively. © 2014 John Wiley & Sons, Ltd.
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Biochemical analysis of Ascites fluid as an aid to etiological diagnosis: a series of 100 cases of nonimmune fetal Ascites.
Prenatal diagnosis, 2014Co-Authors: Sophie Dreux, Laurent Salomon, Jonathan Rosenblatt, Romain Favre, Véronique Houfflin-debarge, Bernard Broussin, Fabien Guimiot, Honorine Fenaux, Anne-lise Delezoide, Françoise MullerAbstract:Objective The aim of this study is to analyze the contribution of biochemistry and cytology of fetal Ascites fluid to the etiological diagnosis of Ascites after ultrasonographic scan, maternal blood sampling, and fetal karyotyping. Method This is a retrospective study of 100 consecutive cases of nonimmune fetal Ascites in which Ascites fluid was sampled. All women underwent referral ultrasound scan and fetal karyotyping. All cases of fetal Ascites were studied by biochemistry (total protein, β2-microglobulin, IgM, gamma-glutamyl transpeptidase, aspartate aminotransferase, aminopeptidase M, and intestinal isoform of alkaline phosphatase) and cytology (lymphocyte count and vacuolated cells). Results The etiology of Ascites was diagnosed at ultrasound scan in only 50% of cases. We observed significantly (P
