The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform
Eva Madrid - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta Fabae Infection
2016Co-Authors: Sara Ocaña, Carmen Palomino, Pedro Seoane, Rocio Bautista, Gonzalo M. Claros, Ana M. Torres, Eva MadridAbstract:Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic infor-mation. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta Fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts dif-ferentially expressed. Comparison of faba bean transcripts with those of better-character-ized plant genomes such as Arabidopsis thaliana,Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8 % and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyp-ing applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and suscep-tible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interaction
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large scale transcriptome analysis in faba bean vicia faba l under Ascochyta Fabae infection
PLOS ONE, 2015Co-Authors: Sara Ocaña, Ana Maria Torres, Carmen Palomino, Pedro Seoane, Rocio Bautista, Gonzalo M. Claros, Eva MadridAbstract:Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic information. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta Fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts differentially expressed. Comparison of faba bean transcripts with those of better-characterized plant genomes such as Arabidopsis thaliana, Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8% and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyping applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and susceptible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interactions such as leucine rich proteins (LRR) or plant growth regulators involved in plant adaptation to abiotic and biotic stresses were found to be differently expressed in the resistant line. The results reported here represent the most comprehensive transcript database developed so far in faba bean, providing valuable information that could be used to gain insight into the pathways involved in the resistance mechanism against A. Fabae and to identify potential resistance genes to be further used in marker assisted selection.
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DeepSuperSage analysis of the Vicia faba transcriptome in response to Ascochyta Fabae infection
Firenze University Press, 2013Co-Authors: Eva Madrid, Carmen Palomino, Anne PlÖtner, Ralf Horres, Björn Rotter, Peter Winter, Nicolas Krezdorn, Ana M. TorresAbstract:Understanding the host response to Ascochyta Fabae in faba bean (Vicia faba L.), is crucial to elucidate the biology of host resistance. In an attempt to unravel the faba bean – A. Fabae interaction, we performed genome-wide transcriptome profiling by deepSuperSAGE that quantified the early transcriptional changes elicited by the fungus in the resistant 29H faba bean genotype. The total number of 26 bp tags obtained was 1,313,009, of which 51,484 were unique sequences (UniTags) and 161 of them corresponded to fungal sequences. Sequences with a full match of the 26 bp revealed 2,222 tags with a significant P-value that were expressed differentialy between inoculated and control leaves. After gene ontology (GO) annotation, 2,143 of these matched to databases sequences (approximately 1/3 into each GO domain). At a 2.7-fold change threshold, 1,197 sequences were significantly differentially expressed in infected as compared to control leaves. Of these, nearly half were up- and the other downregulated. The most enriched GO terms corresponded to tags related with photosynthesis metabolism or structural components. Ten of them can be associated with plant defense, due to their association with responses to the jasmonic acid pathway, pectin esterase activity or gene silencing. Validation of the SuperSAGE data by qPCR of ten differentially expressed UniTags confirmed a rapid increase or decrease in mRNA 8 to 12 hours after inoculation in most of the up-regulated tags and, less consistently, in the downregulated ones. This study represents the most comprehensive analysis of the Ascochyta-response transcriptome of faba bean available to date. The applicability of these tags will increase as more faba bean genomic and cDNA sequences become available
Diego Rubiales - One of the best experts on this subject based on the ideXlab platform.
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induction of systemic acquired resistance against rust Ascochyta blight and broomrape in faba bean by exogenous application of salicylic acid and benzothiadiazole
Crop Protection, 2012Co-Authors: Josefina C. Sillero, Carmen M. Ávila, Maria M Rojasmolina, Diego RubialesAbstract:Abstract Exogenous applications of salicylic acid (SA) and benzothiadiazole (BTH) solutions have been used in faba bean to induce systemic acquired resistance (SAR) to rust ( Uromyces viciae-Fabae ), Ascochyta blight ( Ascochyta Fabae ) and broomrape ( Orobanche crenata ). Both SA and BTH solutions were effective inducing SAR to U. viciae-Fabae and A. Fabae on susceptible accessions under controlled conditions, although SA was less effective than BTH for A. Fabae . BTH treatments reduced the infection of all pathogens studied under field conditions in susceptible accessions, and rust infection was also reduced by SA applications. Moderately resistant accessions became immune to Ascochyta blight with BTH treatment, and showed a lower degree of infection to rust after SA or BTH treatments. No effect was observed in the highly resistant accessions. Chemical induction of systemic resistance may provide an additional method for controlling faba bean diseases to be considered in an integrated diseases management.
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identification and multi environment validation of resistance to Ascochyta Fabae in faba bean vicia faba
Field Crops Research, 2012Co-Authors: Diego Rubiales, Josefina C. Sillero, Carmen M. Ávila, M Hybl, L Narits, Olaf Sass, Fernando FloresAbstract:Financial support by the European Union EUFABA project (ref. QLK5-CT2002-02307) is acknowledged.
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Occurrence of Didymella Fabae, the Teleomorph of Ascochyta Fabae, on Faba Bean Straw in Spain
Journal of Phytopathology, 2002Co-Authors: Diego Rubiales, A. Trapero-casasAbstract:Pseudothecia of Didymella Fabae, the teleomorph of Ascochyta Fabae, were first observed on faba bean (Vicia faba) debris in Spain during autumn 1995. Most pseudothecia were mature by December–February. The ascospores gave rise to typical cultures of A. Fabae, and conidia from these cultures caused Ascochyta blight symptoms on inoculated faba bean plants. Placing straw-bearing pseudothecia over the plants to allow ascospore discharge also resulted in typical Ascochyta blight symptoms. Pseudothecia maturity and discharge of ascospores from the infested faba bean straw overlapped with the vegetative stage of the faba bean crop, which occurs in southern Spain during winter as the crop is sown in autumn and harvested in spring. These observations indicate that ascospores may serve as primary inoculum for the disease.
Ana Maria Torres - One of the best experts on this subject based on the ideXlab platform.
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saturation mapping of regions determining resistance to Ascochyta blight and broomrape in faba bean using transcriptome based snp genotyping
Theoretical and Applied Genetics, 2017Co-Authors: S Ocanamoral, Ana Maria Torres, N Gutierrez, E MadridAbstract:Transcriptome-based SNP markers were genotyped in a faba bean map to saturate regions bearing QTL for Ascochyta Fabae and broomrape and distinguish positional and functional candidates underlying both resistances. Faba bean is an important food crop worldwide. Marker-assisted selection for disease resistance is a top priority in current faba bean research programs, with pathogens such as Ascochyta Fabae and broomrape (Orobanche crenata) being among the major constraints in global faba bean production. However, progress in genetics and genomics in this species has lagged behind that of other grain legumes. Although genetic maps are available, most markers are not in or are too distant from target genes to enable an accurate prediction of the desired phenotypes. In this study, a set of SNP markers located in gene coding regions was selected using transcriptomic data. Ninety-two new SNP markers were genotyped to obtain the most complete map reported so far in the 29H × Vf136 faba bean population. Most of the QTL regions previously described in this cross were enriched with SNP markers. Two QTLs for O. crenata resistance (Oc7 and Oc8) were confirmed. Oc7 and Oc10 located nearby a QTL for A. Fabae resistance suggested that these genomic regions might encode common resistance mechanisms and could be targets for selection strategies against both pathogens. We also confirmed three regions in chromosomes II (Af2), III (Af3) and VI associated with Ascochyta blight resistance. The QTLs ratified in the present study are now flanked by or include reliable SNP markers in their intervals. This new information provides a valuable starting point in the search for relevant positional and functional candidates underlying both types of resistance.
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qtls for Ascochyta blight resistance in faba bean vicia faba l validation in field and controlled conditions
Crop & Pasture Science, 2016Co-Authors: Sergio G Atienza, Carmen Alfaro, Ana Maria Torres, Carmen Palomino, Natalia Gutierrez, Carmen M. ÁvilaAbstract:Ascochyta blight is an important disease of faba bean (Vicia faba L.). Yield losses can be as high as 90% and losses of 35–40% are common. The line 29H is one of the most resistant accessions to the pathogen (Ascochyta Fabae Speg.) ever described. In this work, we aimed to validate across generations the main quantitative trait loci (QTLs) for Ascochyta blight resistance identified in the cross 29H × Vf136 and to test their stability under field conditions. QTLs located on chromosomes II and III have been consistently identified in the recombinant inbred line (RIL) population of this cross, in both controlled (growth chamber) and field conditions and, thus they are good targets for breeding. In addition, a new QTL for disease severity on pods has been located on chromosome VI, but in this case, further validation is still required.A synteny-based approach was used to compare our results with previous QTL works dealing with this pathogen. Our results suggest that the QTL located on chromosome II, named Af2, is the same one reported by other researchers, although it is likely that the donors of resistance differ in the allele conferring the resistance. By contrast, the location of Af3 on chromosome III does not overlap with the position of Af1 reported by other authors, suggesting that Af3 may be an additional source of resistance to Ascochyta blight.
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large scale transcriptome analysis in faba bean vicia faba l under Ascochyta Fabae infection
PLOS ONE, 2015Co-Authors: Sara Ocaña, Ana Maria Torres, Carmen Palomino, Pedro Seoane, Rocio Bautista, Gonzalo M. Claros, Eva MadridAbstract:Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic information. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta Fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts differentially expressed. Comparison of faba bean transcripts with those of better-characterized plant genomes such as Arabidopsis thaliana, Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8% and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyping applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and susceptible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interactions such as leucine rich proteins (LRR) or plant growth regulators involved in plant adaptation to abiotic and biotic stresses were found to be differently expressed in the resistant line. The results reported here represent the most comprehensive transcript database developed so far in faba bean, providing valuable information that could be used to gain insight into the pathways involved in the resistance mechanism against A. Fabae and to identify potential resistance genes to be further used in marker assisted selection.
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confirmation of qtls controlling Ascochyta Fabae resistance in different generations of faba bean vicia faba l
Crop & Pasture Science, 2009Co-Authors: Ramon Diazruiz, Carmen M. Ávila, Carmen Alfaro, Ana Maria Torres, Zlatko Satovic, Maria Victoria Gutierrez, Belen RomanAbstract:Ascochyta blight, caused by Ascochyta Fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta Fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6 × Vf136) in which A. Fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F2 population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7 cM, with a mean inter-marker distance of 12.72 cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for Ascochyta blight resistance found in F3 families in the derived RILs (F6), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.
Torres, Ana M. - One of the best experts on this subject based on the ideXlab platform.
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QTLs for Ascochyta blight resistance in faba bean (Vicia faba L.): validation in field and controlled conditions
'CSIRO Publishing', 2016Co-Authors: Atienza, Sergio G., Rubiales Diego, Palomino Carmen, Torres, Ana M., Gutiérrez Natalia, Alfaro C. M., Ávila, Carmen M.Abstract:Ascochyta blight is an important disease of faba bean (Vicia faba L.). Yield losses can be as high as 90% and losses of 35–40% are common. The line 29H is one of the most resistant accessions to the pathogen (Ascochyta Fabae Speg.) ever described. In this work, we aimed to validate across generations the main quantitative trait loci (QTLs) for Ascochyta blight resistance identified in the cross 29H × Vf136 and to test their stability under field conditions. QTLs located on chromosomes II and III have been consistently identified in the recombinant inbred line (RIL) population of this cross, in both controlled (growth chamber) and field conditions and, thus they are good targets for breeding. In addition, a new QTL for disease severity on pods has been located on chromosome VI, but in this case, further validation is still required. A synteny-based approach was used to compare our results with previous QTL works dealing with this pathogen. Our results suggest that the QTL located on chromosome II, named Af2, is the same one reported by other researchers, although it is likely that the donors of resistance differ in the allele conferring the resistance. By contrast, the location of Af3 on chromosome III does not overlap with the position of Af1 reported by other authors, suggesting that Af3 may be an additional source of resistance to Ascochyta blight.Peer reviewe
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Análisis de la expresión diferencial de cDNAs y mapeo de genes candidatos para saturar QTLs de resistencia a Ascochyta en habas (Vicia faba L.)
2015Co-Authors: Ocaña-moral Sara, Madrid Eva, Seoane Pedro, Bautista Rocío, Gonzalo Claros M., Torres, Ana M.Abstract:Trabajo presentado en el XL Congreso de la Sociedad Española de Genética, celebrado en Córdoba del 16 al 18 de septiembre de 2015.Aunque las habas (Vicia faba L.) son un importante cultivo alimentario en todo el mundo y una fuente de proteínas en la dieta de países en vías de desarrollo, su información genética y genómica están aún muy por detrás de las especies modelo. Con el fin de contribuir en su progreso, en este estudio se ha realizado una caracterización exhaustiva del transcriptoma de dos genotipos de haba (29H y Vf136) con reacción diferencial frente a la infección por el hongo Ascochyta Fabae utilizando la plataforma de secuenciación Illumina. A partir de la información obtenida tras el ensamblaje del nuevo transcriptoma se han identificado 21.243 unigenes que han sido anotados con diferentes bases de datos. De ellos se ha obtenido un subconjunto de 850 expresados diferencialmente entre el parental resistente (29H) y el susceptible (Vf136) a p < 0.05. Además se han identificado 39.060 SNPs y 3.669 INDELs. De los 850 unigenes se han seleccionado 128 por presentar algún SNP y se utilizaron para genotipar los individuos de esta población mediante los sistemas de genotipado KASPar e iPLEX-Sequenom. Estos unigenes expresados diferencialmente tras la infección del hongo Ascochyta Fabae pueden identificar genes candidatos asociados a la resistencia a estreses bióticos en esta leguminosa. La integración de estos genes en el mapa de referencia disponible nos ayudará a actualizar el mapa de ligamiento de la población y a saturar QTLs relacionados con la resistencia a Ascochyta identificados en estudios previos (1, 2, 3). Los resultados serán un importante recurso para futuros estudios básicos y aplicados en leguminosas de importancia económica, proporcionand o información valiosa para comprender mejor las vías involucradas en el mecanismo de resistencia contra A. Fabae y para identificar genes de resistencia potenciales para ser utilizado en la selección asistida por marcadores.N
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Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta Fabae Infection
'Public Library of Science (PLoS)', 2015Co-Authors: Ocaña-moral Sara, Palomino Carmen, Torres, Ana M., Seoane Pedro, Bautista Rocío, Claros, Gonzalo M., Madrid EvaAbstract:Faba bean is an important food crop worldwide. However, progress in faba bean genomics lags far behind that of model systems due to limited availability of genetic and genomic information. Using the Illumina platform the faba bean transcriptome from leaves of two lines (29H and Vf136) subjected to Ascochyta Fabae infection have been characterized. De novo transcriptome assembly provided a total of 39,185 different transcripts that were functionally annotated, and among these, 13,266 were assigned to gene ontology against Arabidopsis. Quality of the assembly was validated by RT-qPCR amplification of selected transcripts differentially expressed. Comparison of faba bean transcripts with those of better-characterized plant genomes such as Arabidopsis thaliana, Medicago truncatula and Cicer arietinum revealed a sequence similarity of 68.3%, 72.8% and 81.27%, respectively. Moreover, 39,060 single nucleotide polymorphism (SNP) and 3,669 InDels were identified for genotyping applications. Mapping of the sequence reads generated onto the assembled transcripts showed that 393 and 457 transcripts were overexpressed in the resistant (29H) and susceptible genotype (Vf136), respectively. Transcripts involved in plant-pathogen interactions such as leucine rich proteins (LRR) or plant growth regulators involved in plant adaptation to abiotic and biotic stresses were found to be differently expressed in the resistant line. The results reported here represent the most comprehensive transcript database developed so far in faba bean, providing valuable information that could be used to gain insight into the pathways involved in the resistance mechanism against A. Fabae and to identify potential resistance genes to be further used in marker assisted selection.This research was supported by the European Community's Seventh Framework Programme (FP7/ 2007-2013) under the grant agreement n°FP7-613551, LEGATO project; the 2014-2020 "Programa Operativo de Crecimiento Inteligente" (RTA2013-00025) and RTA2010-00059, the IPT-2011-1259-010000 project and the P10-CVI-6075, all co-financed by ERDF and the last by the Andalusian regional government.Peer reviewe
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Large-scale transcriptome analysis in faba bean (Vicia faba L.)
2014Co-Authors: Ocaña-moral Sara, Torres, Ana M., Seoane Pedro, Bautista Rocío, Claros, Gonzalo M., Madrid EvaAbstract:Trabajo presentado en la 6th International Food Legume Research Conference y la 7th International Conference of Legume Genetics and Genomics (IFLRC VI & ICLGG VII), celebrada en Saskatoon (Canadá) del 7 al 11 de julio de 2014.Although faba bean is one of the biggest traded pulse crops, genomic resources are still limited. A comprehensive characterization of the faba bean transcriptome was performed using the Illumina platform. Two faba bean genotypes (Vf29H and Vf136) with differential reaction to Ascochyta Fabae and 106.397 ESTs found in different public databases were used for de novo transcriptome assembly using a work flow with OASES, and CAP3. The resulting unigenes were annotated with Full-LengtherNext. The preliminary analysis of the 120.922 unigenes collection revealed that 20,804 out of the 39,269 were non-redundant transcripts based on orthologue ID. 2,752putative ncRNAs were found. Futhermore, 7,165 unigenes lacking homology could be considered as putative legume-specific genes. Single nucleotide polymorphisms identified through data mining provide a database for genotyping and integration of translational genomics in breeding programmes. In addition, the faba bean and chickpea transcriptome alignment revealed that 81.27% of the faba bean transcripts where present in the chickpea transcriptome despite of using a single faba bean source of tissue. As the genotypes sequenced show differential reaction to A. Fabae and the cDNA libraries were constructed using inoculated and non-inoculated leaves, genes with differential expression in both genotypes were characterized. Thus, 348 and 327 transcripts where found to be unique in resistant and susceptible genotypes, respectively. On the other hand, 212 and 282 transcripts were found to be overexpressed in both lines. These transcripts might represent candidate genes associated with the A. Fabae resistance.N
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Deepsupersage analysis of the Vicia faba transcriptome in response to Ascochyta Fabae infection
'Firenze University Press', 2013Co-Authors: Madrid Eva, Palomino Carmen, Plötner Anne, Horres Ralf, Rotter Björn, Winter Peter, Krezdorn Nicolas, Torres, Ana M.Abstract:Understanding the host response to Ascochyta Fabae in faba bean (Vicia faba L.), is crucial to elucidate the biology of host resistance. In an attempt to unravel the faba bean - A. Fabae interaction, we performed genome-wide transcriptome profiling by deepSuperSAGE that quantified the early transcriptional changes elicited by the fungus in the resistant29H faba bean genotype. The total number of 26 bp tags obtained was 1,313,009, of which 51,484 were unique sequences (UniTags) and 161 of them corresponded to fungal sequences. Sequences with a full match of the 26 bp revealed 2,222 tags with a significant P-value that were expressed differentialy between inoculated and control leaves. After gene ontology (GO) annotation, 2,143 of these matched to databases sequences (approximately 1/3 into each GO domain). At a 2.7-fold change threshold, 1,197 sequences were significantly differentially expressed in infected as compared to control leaves. Of these, nearly half were up- and the other downregulated. The most enriched GO terms corresponded to tags related with photosynthesis metabolism or structural components. Ten of them can be associated with plant defense, due to their association with responses to the jasmonic acid pathway, pectin esterase activity or gene silencing. Validation of the SuperSAGE data by qPCR of ten differentially expressed UniTags confirmed a rapid increase or decrease in mRNA 8 to 12 hours after inoculation in most of the up-regulated tags and, less consistently, in the downregulated ones. This study represents the most comprehensive analysis of the Ascochyta-response transcriptome of faba bean available to date. The applicability of these tags will increase as more faba bean genomic and cDNA sequences become available. © Firenze University Press.This research was supported by the ERA-NET Plant Genomics program, project LEGRESIST (GEN2006-27798-C6-5-/VEG) and by the Spanish MICINN project RTA2010-00059, co-financed with FEDER.Peer Reviewe
Carmen M. Ávila - One of the best experts on this subject based on the ideXlab platform.
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qtls for Ascochyta blight resistance in faba bean vicia faba l validation in field and controlled conditions
Crop & Pasture Science, 2016Co-Authors: Sergio G Atienza, Carmen Alfaro, Ana Maria Torres, Carmen Palomino, Natalia Gutierrez, Carmen M. ÁvilaAbstract:Ascochyta blight is an important disease of faba bean (Vicia faba L.). Yield losses can be as high as 90% and losses of 35–40% are common. The line 29H is one of the most resistant accessions to the pathogen (Ascochyta Fabae Speg.) ever described. In this work, we aimed to validate across generations the main quantitative trait loci (QTLs) for Ascochyta blight resistance identified in the cross 29H × Vf136 and to test their stability under field conditions. QTLs located on chromosomes II and III have been consistently identified in the recombinant inbred line (RIL) population of this cross, in both controlled (growth chamber) and field conditions and, thus they are good targets for breeding. In addition, a new QTL for disease severity on pods has been located on chromosome VI, but in this case, further validation is still required.A synteny-based approach was used to compare our results with previous QTL works dealing with this pathogen. Our results suggest that the QTL located on chromosome II, named Af2, is the same one reported by other researchers, although it is likely that the donors of resistance differ in the allele conferring the resistance. By contrast, the location of Af3 on chromosome III does not overlap with the position of Af1 reported by other authors, suggesting that Af3 may be an additional source of resistance to Ascochyta blight.
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induction of systemic acquired resistance against rust Ascochyta blight and broomrape in faba bean by exogenous application of salicylic acid and benzothiadiazole
Crop Protection, 2012Co-Authors: Josefina C. Sillero, Carmen M. Ávila, Maria M Rojasmolina, Diego RubialesAbstract:Abstract Exogenous applications of salicylic acid (SA) and benzothiadiazole (BTH) solutions have been used in faba bean to induce systemic acquired resistance (SAR) to rust ( Uromyces viciae-Fabae ), Ascochyta blight ( Ascochyta Fabae ) and broomrape ( Orobanche crenata ). Both SA and BTH solutions were effective inducing SAR to U. viciae-Fabae and A. Fabae on susceptible accessions under controlled conditions, although SA was less effective than BTH for A. Fabae . BTH treatments reduced the infection of all pathogens studied under field conditions in susceptible accessions, and rust infection was also reduced by SA applications. Moderately resistant accessions became immune to Ascochyta blight with BTH treatment, and showed a lower degree of infection to rust after SA or BTH treatments. No effect was observed in the highly resistant accessions. Chemical induction of systemic resistance may provide an additional method for controlling faba bean diseases to be considered in an integrated diseases management.
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identification and multi environment validation of resistance to Ascochyta Fabae in faba bean vicia faba
Field Crops Research, 2012Co-Authors: Diego Rubiales, Josefina C. Sillero, Carmen M. Ávila, M Hybl, L Narits, Olaf Sass, Fernando FloresAbstract:Financial support by the European Union EUFABA project (ref. QLK5-CT2002-02307) is acknowledged.
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confirmation of qtls controlling Ascochyta Fabae resistance in different generations of faba bean vicia faba l
Crop & Pasture Science, 2009Co-Authors: Ramon Diazruiz, Carmen M. Ávila, Carmen Alfaro, Ana Maria Torres, Zlatko Satovic, Maria Victoria Gutierrez, Belen RomanAbstract:Ascochyta blight, caused by Ascochyta Fabae Speg., is a disease of faba bean (Vicia faba L.) of worldwide distribution. In this study we have conducted an experiment on Ascochyta Fabae resistance in 165 recombinant inbred lines (RILs) developed by single-seed descent from the cross between resistant and susceptible lines (Vf6 × Vf136) in which A. Fabae resistance QTLs (quantitative trait loci) have been previously reported in the original F2 population. Recombinant inbred lines were inoculated under controlled growth chamber conditions and evaluated for disease severity and infection type index. The linkage map was constructed by MAPMAKER V2.0 and the QTL analysis was carried out using QTL Cartographer. Two hundred and seventy-seven markers (238 RAPDs, 4 isozymes, 5 ESTs, 1 SCAR, 6 SSRs, 2 STSs, and 21 intron-spanning markers) mapped into 21 linkage groups covering 2.856.7 cM, with a mean inter-marker distance of 12.72 cM. Composite interval mapping identified two zones of putative QTL action in the RIL population for DSL (disease severity on leaves) and DSS (disease severity on stems) traits. Putative QTLs (Af1 and Af2) were identified on chromosome 3 and chromosome 2, respectively, and jointly explained 24% of the phenotypic variance of DSL and 16% of DSS. With this study we have (1) confirmed the QTLs for Ascochyta blight resistance found in F3 families in the derived RILs (F6), (2) re-estimated their position and genetic effects, and (3) assessed the stability of these QTLs in different genetic backgrounds by comparison of the mapping data with a previous QTL study.
