The Experts below are selected from a list of 243 Experts worldwide ranked by ideXlab platform
Michelle S Tierney - One of the best experts on this subject based on the ideXlab platform.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
Cédric Leroux - One of the best experts on this subject based on the ideXlab platform.
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Trophic ecology of the rocky shore community associated with the Ascophyllum nodosum zone (Roscoff, France): A delta C-13 vs delta N-15 investigation
Estuarine Coastal and Shelf Science, 2009Co-Authors: Pascal Riera, Carole Escaravage, Cédric LerouxAbstract:This study aimed to characterize the structure and functioning of the benthic food web associated with the Ascophyllum nodosum zone of the rocky shore of Roscoff by using delta C-13 and delta N-15. Several characteristics of the trophic ecology of the invertebrates associated with this mid-littoral habitat and which belong to different functional groups (e.g., grazers, filter-feeders, predators and omnivores) were highlighted. In particular, the filter feeder species (including mostly sponges) used macroalgae-derived organic matter as a substantial food requirement. The results also pointed out an important stable isotopes variability for strict coexisting primary consumers which: (1) is directly related to the high delta N-15 range of the food sources; (2) makes it impossible to establish a unique trophic level scale based on delta N-15 values, as previously done in coastal environments; and (3) points out the existence of major co-occurring trophic pathways which characterise the Ascophyllum nodosum habitat. (C) 2008 Elsevier Ltd. All rights reserved.
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trophic ecology of the rocky shore community associated with the Ascophyllum nodosum zone roscoff france a δ13c vs δ15n investigation
Estuarine Coastal and Shelf Science, 2009Co-Authors: Pascal Riera, Carole Escaravage, Cédric LerouxAbstract:Abstract This study aimed to characterize the structure and functioning of the benthic food web associated with the Ascophyllum nodosum zone of the rocky shore of Roscoff by using δ13C and δ15N. Several characteristics of the trophic ecology of the invertebrates associated with this mid-littoral habitat and which belong to different functional groups (e.g., grazers, filter-feeders, predators and omnivores) were highlighted. In particular, the filter feeder species (including mostly sponges) used macroalgae-derived organic matter as a substantial food requirement. The results also pointed out an important stable isotopes variability for strict coexisting primary consumers which: (1) is directly related to the high δ15N range of the food sources; (2) makes it impossible to establish a unique trophic level scale based on δ15N values, as previously done in coastal environments; and (3) points out the existence of major co-occurring trophic pathways which characterise the Ascophyllum nodosum habitat.
Thomas J Smyth - One of the best experts on this subject based on the ideXlab platform.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
Anna K Croft - One of the best experts on this subject based on the ideXlab platform.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
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uplc ms profiling of low molecular weight phlorotannin polymers in Ascophyllum nodosum pelvetia canaliculata and fucus spiralis
Metabolomics, 2014Co-Authors: Michelle S Tierney, Anna Solervila, Anna K Croft, Nigel P Brunton, Thomas J SmythAbstract:Phlorotannins are a group of complex polymers, found in particular brown macroalgae, composed solely of the monomer phloroglucinol (1,3,5-trihydroxybenzene). Their structural complexity arises from the number of possible linkage positions between each monomer unit. This study aimed to profile the phlorotannin metabolite composition and the complexity of isomerisation present in brown macroalgae Ascophyllum nodosum, Pelvetia canaliculata and Fucus spiralis using UPLC-MS utilising a tandem quadrupole mass spectrometer. Phlorotannin-enriched fractions from water and aqueous ethanol extracts were analysed by UPLC-MS performed in multiple reaction monitoring mode to detect molecular ions consistent with the molecular weights of phlorotannins. Ascophyllum nodosum and P. canaliculata appeared to contain predominantly larger phlorotannins (degree of polymerisation (DP) of 6–13 monomers) compared to F. spiralis (DP of 4–6 monomers). This is the first report observing the complex chromatographic separation and metabolomic profiling of low molecular weight phlorotannins consisting of more than ten monomers. Extracted ion chromatograms, for each of the MRM transitions, for each species were analysed to profile the level of isomerisation for specific molecular weights of phlorotannins between 3 and 16 monomers. The level of phlorotannin isomerisation within the extracts of the individual macroalgal species differed to some degree, resulting in substantially different numbers of phlorotannin isomers for particular molecular weights. A similar UPLC-MS/MS separation procedure, as outlined in this study, may be used in the future as a means of screening the metabolite profile of macroalgal extracts, therefore, allowing extract consistency to be monitored for standardisation purposes.
Pascal Riera - One of the best experts on this subject based on the ideXlab platform.
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Trophic ecology of the rocky shore community associated with the Ascophyllum nodosum zone (Roscoff, France): A delta C-13 vs delta N-15 investigation
Estuarine Coastal and Shelf Science, 2009Co-Authors: Pascal Riera, Carole Escaravage, Cédric LerouxAbstract:This study aimed to characterize the structure and functioning of the benthic food web associated with the Ascophyllum nodosum zone of the rocky shore of Roscoff by using delta C-13 and delta N-15. Several characteristics of the trophic ecology of the invertebrates associated with this mid-littoral habitat and which belong to different functional groups (e.g., grazers, filter-feeders, predators and omnivores) were highlighted. In particular, the filter feeder species (including mostly sponges) used macroalgae-derived organic matter as a substantial food requirement. The results also pointed out an important stable isotopes variability for strict coexisting primary consumers which: (1) is directly related to the high delta N-15 range of the food sources; (2) makes it impossible to establish a unique trophic level scale based on delta N-15 values, as previously done in coastal environments; and (3) points out the existence of major co-occurring trophic pathways which characterise the Ascophyllum nodosum habitat. (C) 2008 Elsevier Ltd. All rights reserved.
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trophic ecology of the rocky shore community associated with the Ascophyllum nodosum zone roscoff france a δ13c vs δ15n investigation
Estuarine Coastal and Shelf Science, 2009Co-Authors: Pascal Riera, Carole Escaravage, Cédric LerouxAbstract:Abstract This study aimed to characterize the structure and functioning of the benthic food web associated with the Ascophyllum nodosum zone of the rocky shore of Roscoff by using δ13C and δ15N. Several characteristics of the trophic ecology of the invertebrates associated with this mid-littoral habitat and which belong to different functional groups (e.g., grazers, filter-feeders, predators and omnivores) were highlighted. In particular, the filter feeder species (including mostly sponges) used macroalgae-derived organic matter as a substantial food requirement. The results also pointed out an important stable isotopes variability for strict coexisting primary consumers which: (1) is directly related to the high δ15N range of the food sources; (2) makes it impossible to establish a unique trophic level scale based on δ15N values, as previously done in coastal environments; and (3) points out the existence of major co-occurring trophic pathways which characterise the Ascophyllum nodosum habitat.
