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Marie-paule Gustin - One of the best experts on this subject based on the ideXlab platform.
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evaluation of hirst type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLOS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal®, bioMerieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair®, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10–4) and Period A vs Period C (p = 10–4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLoS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal\textregistered, bioMérieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair\textregistered, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10-4) and Period A vs Period C (p = 10-4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
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Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
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Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
Philippe Vanhems - One of the best experts on this subject based on the ideXlab platform.
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evaluation of hirst type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLOS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal®, bioMerieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair®, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10–4) and Period A vs Period C (p = 10–4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLoS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal\textregistered, bioMérieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair\textregistered, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10-4) and Period A vs Period C (p = 10-4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
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Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
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Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
Sophie Tiphaine Loeffert - One of the best experts on this subject based on the ideXlab platform.
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evaluation of hirst type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLOS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal®, bioMerieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair®, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10–4) and Period A vs Period C (p = 10–4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLoS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal\textregistered, bioMérieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair\textregistered, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10-4) and Period A vs Period C (p = 10-4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
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Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
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Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
Cédric Dananché - One of the best experts on this subject based on the ideXlab platform.
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evaluation of hirst type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLOS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal®, bioMerieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair®, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10–4) and Period A vs Period C (p = 10–4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Evaluation of Hirst-type spore traps in outdoor Aspergillaceae monitoring during large demolition work in hospital
PLoS ONE, 2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork. Outdoor Aspergillaceae fungal load was monitored by cultivable (Air Ideal\textregistered, bioMérieux) and non-cultivable methods (Lanzoni VPPS-2000, Analyzair\textregistered, Bologna, Italy). Differences of Aspergillaceae recorded with Hirst-type spore traps were observed between Gerland and Edouard Herriot Hospital. Differences between Aspergillaceae were recorded between day time and night time at Gerland and Edouard Herriot Hospital. Daily paired differences between Aspergillaceae recorded with non-cultivable methodology at Edouard Herriot Hospital and in an area without demolition work were significant in Period A vs Period B (p = 10-4) and Period A vs Period C (p = 10-4). Weak correlation of daily Aspergillaceae recorded by both methods at Edouard Herriot Hospital was significant only for Period C (r = 0.26, p = 0.048, n = 58). Meteorological parameters and type of demolition works were found to heavily influenced Aspergillaceae dispersion. Non-cultivable methodology is a promising tool for outdoor Aspergillaceae scrutiny during major demolition work in hospital, helping infection control staff to rapidly implement control measures.
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Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Median of Aspergillaceae levels every 2-h by the non-cultivable method at Gerland and EHH during the 3 demolition periods.
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Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Aspergillaceae fungal loads in outdoor air sampled by cultivable (CFU/m3) and non-cultivable (spores/m3/day) methods in the 3 demolition periods.
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Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
2018Co-Authors: Sophie Tiphaine Loeffert, Estelle Tissot, Thomas Benet, M. Perraud, Philippe Cassier, Michel Thibaudon, Cédric Dananché, Philippe Vanhems, Marie-paule GustinAbstract:Daily average concentrations of Aspergillaceae spores and colonies (spores/m3/day and CFU/m3) recorded by cultivable and non-cultivable methods during demolition at EHH, in 2015.
Gerald F. Bills - One of the best experts on this subject based on the ideXlab platform.
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Acrophiarin (antibiotic S31794/F-1) from Penicillium arenicola shares biosynthetic features with both Aspergillus- and Leotiomycete-type echinocandins
Environmental microbiology, 2020Co-Authors: Nan Lan, Bruno Perlatti, Daniel J Kvitek, Philipp Wiemann, Colin J B Harvey, Jens Christian Frisvad, Gerald F. BillsAbstract:The antifungal echinocandin lipopeptide, acrophiarin, was circumscribed in a patent in 1979. We confirmed that the producing strain NRRL 8095 is Penicillium arenicola and other strains of P. arenicola produced acrophiarin and acrophiarin analogues. Genome sequencing of NRRL 8095 identified the acrophiarin gene cluster. Penicillium arenicola and echinocandin-producing Aspergillus species belong to the family Aspergillaceae of the Eurotiomycetes, but several features of acrophiarin and its gene cluster suggest a closer relationship with echinocandins from Leotiomycete fungi. These features include hydroxy-glutamine in the peptide core instead of a serine or threonine residue, the inclusion of a non-heme iron, α-ketoglutarate-dependent oxygenase for hydroxylation of the C3 of the glutamine, and a thioesterase. In addition, P. arenicola bears similarity to Leotiomycete echinocandin-producing species because it exhibits self-resistance to exogenous echinocandins. Phylogenetic analysis of the genes of the echinocandin biosynthetic family indicated that most of the predicted proteins of acrophiarin gene cluster exhibited higher similarity to the predicted proteins of the pneumocandin gene cluster of the Leotiomycete Glarea lozoyensis than to those of the echinocandin B gene cluster from A. pachycristatus. The fellutamide gene cluster and related gene clusters are recognized as relatives of the echinocandins. Inclusion of the acrophiarin gene cluster into a comprehensive phylogenetic analysis of echinocandin gene clusters indicated the divergent evolutionary lineages of echinocandin gene clusters are descendants from a common ancestral progenitor. The minimal 10-gene cluster may have undergone multiple gene acquisitions or losses and possibly horizontal gene transfer after the ancestral separation of the two lineages.
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acrophiarin antibiotic s31794 f 1 from penicillium arenicola shares biosynthetic features with both aspergillus and leotiomycete type echinocandins
Environmental Microbiology, 2020Co-Authors: Bruno Perlatti, Daniel J Kvitek, Philipp Wiemann, Colin J B Harvey, Zhiqiang An, Jens Christian Frisvad, Gerald F. BillsAbstract:: The antifungal echinocandin lipopeptide, acrophiarin, was circumscribed in a patent in 1979. We confirmed that the producing strain NRRL 8095 is Penicillium arenicola and other strains of P. arenicola produced acrophiarin and acrophiarin analogues. Genome sequencing of NRRL 8095 identified the acrophiarin gene cluster. Penicillium arenicola and echinocandin-producing Aspergillus species belong to the family Aspergillaceae of the Eurotiomycetes, but several features of acrophiarin and its gene cluster suggest a closer relationship with echinocandins from Leotiomycete fungi. These features include hydroxy-glutamine in the peptide core instead of a serine or threonine residue, the inclusion of a non-heme iron, α-ketoglutarate-dependent oxygenase for hydroxylation of the C3 of the glutamine, and a thioesterase. In addition, P. arenicola bears similarity to Leotiomycete echinocandin-producing species because it exhibits self-resistance to exogenous echinocandins. Phylogenetic analysis of the genes of the echinocandin biosynthetic family indicated that most of the predicted proteins of acrophiarin gene cluster exhibited higher similarity to the predicted proteins of the pneumocandin gene cluster of the Leotiomycete Glarea lozoyensis than to those of the echinocandin B gene cluster from A. pachycristatus. The fellutamide gene cluster and related gene clusters are recognized as relatives of the echinocandins. Inclusion of the acrophiarin gene cluster into a comprehensive phylogenetic analysis of echinocandin gene clusters indicated the divergent evolutionary lineages of echinocandin gene clusters are descendants from a common ancestral progenitor. The minimal 10-gene cluster may have undergone multiple gene acquisitions or losses and possibly horizontal gene transfer after the ancestral separation of the two lineages.
