Aspiculuris tetraptera

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Catherine Moulia - One of the best experts on this subject based on the ideXlab platform.

  • Comparison between patterns of pinworm infection (Aspiculuris tetraptera) in wild and laboratory strains of mice, Mus musculus.
    International journal for parasitology, 1997
    Co-Authors: Jean-marc Derothe, Claude Loubes, Annie Orth, François Renaud, Catherine Moulia
    Abstract:

    Sixteen laboratory and 7 wild-derived strains of mice were infected with the pinworm Aspiculuris tetraptera in order to compare their resistance levels estimated by the intestinal parasite loads. It appears that (i) in 4 strains out of 23, females and males harbour different parasite loads; (ii) wild and laboratory mice display a broad range of infection levels when compared independently; (iii) the laboratory strains are more resistant than the wild ones. We suggest that (i) compared to sex, the strain (i.e. genetic) effect is the main parameter which determines the levels of infection; (ii) resistance was selected in laboratory strains during their breeding because of the parasite pressure present in captivity.

  • experimental evidence of genetic determinism in high susceptibility to intestinal pinworm infection in mice a hybrid zone model
    Parasitology, 1993
    Co-Authors: Catherine Moulia, Le N Brun, J Dallas, A Orth, F Renaud
    Abstract:

    In the hybrid zone of the two mouse subspecies Mus musculus musculus and Mus musculus domesticus , mice with hybrid genotypes harbour, on the average, more helminth parasites (cestodes and nematodes) than mice of the two parental taxa. In order to determine the roles played by genetic parameters in this phenomenon, mice with recombined and parental genotypes were experimentally infected with the intestinal pinworm Aspiculuris tetraptera , a natural parasite of the house mouse. The results showed that the high susceptibility of the hybrid zone mice is genetically determined. In addition, this study shows the occurrence of variability among resistant parental populations.

Teresa Cristina Bergamo Do Bomfim - One of the best experts on this subject based on the ideXlab platform.

Hong-kean Ooi - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification.
    Veterinary parasitology, 2008
    Co-Authors: Joan Dee C Parel, Jedhan U Galula, Hong-kean Ooi
    Abstract:

    To differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata and Syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 (ITS-1), 5.8S gene, and ITS-2 of the ribosomal DNA followed by designing of species-specific primers for future use in the identification of the worms. It was observed that S. obvelata, S. muris and Aspiculuris tetraptera can be differentiated from each other based on their rDNA sequences. This is the first report of the ITS-1, 5.8S, and ITS-2 of the rDNA of the three aforementioned rodent pinworm species. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species. Moreover, we also constructed species-specific primers that were designed for unique regions of the ITS-2 of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogenous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice, and wild rodents.

Sandra Lucia Da Cunha E Silva - One of the best experts on this subject based on the ideXlab platform.

Joan Dee C Parel - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification.
    Veterinary parasitology, 2008
    Co-Authors: Joan Dee C Parel, Jedhan U Galula, Hong-kean Ooi
    Abstract:

    To differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata and Syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 (ITS-1), 5.8S gene, and ITS-2 of the ribosomal DNA followed by designing of species-specific primers for future use in the identification of the worms. It was observed that S. obvelata, S. muris and Aspiculuris tetraptera can be differentiated from each other based on their rDNA sequences. This is the first report of the ITS-1, 5.8S, and ITS-2 of the rDNA of the three aforementioned rodent pinworm species. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species. Moreover, we also constructed species-specific primers that were designed for unique regions of the ITS-2 of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogenous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice, and wild rodents.