The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Geum-sook Hwang - One of the best experts on this subject based on the ideXlab platform.
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using 1H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the 1H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of 1H NMR and UPLC-MS analyses can be used to eval...
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of agricultural and food chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Jin-sup Kim, Hyun Ryu, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using (1)H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the (1)H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of (1)H NMR and UPLC-MS analyses can be used to evaluate peeling procedures used in the postharvest processing of herbal medicines.
Jeeyoun Jung - One of the best experts on this subject based on the ideXlab platform.
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using 1H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the 1H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of 1H NMR and UPLC-MS analyses can be used to eval...
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of agricultural and food chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Jin-sup Kim, Hyun Ryu, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using (1)H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the (1)H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of (1)H NMR and UPLC-MS analyses can be used to evaluate peeling procedures used in the postharvest processing of herbal medicines.
Ming Zhao - One of the best experts on this subject based on the ideXlab platform.
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Astragalus Root dry extract restores connexin43 expression by targeting mir 1 in viral myocarditis
Phytomedicine, 2018Co-Authors: Yu Wang, Li-ying Xuan, Yongfeng Liu, Liqun Shao, Cheng-xi Wei, Ming ZhaoAbstract:Abstract Background Viral myocarditis is defined as viral infection of myocardial tissue leading to impaired heart function and heart failure. Accumulating evidences have shown that arrhythmia is one of important complicating diseases of viral myocarditis causing increased mortality and morbidity. There are no effective treatment for the viral infection and complicating arrhythmia. Purpose This study investigated the effect and mechanism of Astragalus Root dry extract (ARDE) on arrhythmia induced by CVB3 in mice. Methods The mice and HL-1 cells were treated with CVB3 and ARDE. Reciprocal regulation of Cx43 and miR-1 were observed in the CVB3 infected mouse myocardium and culture HL-1 cells. Results CVB3 IP injection increased immune cell infiltration in mouse left ventricle and caused irregular arrhythmia. ARDE treatment prevented the increase of immune cell infiltration and arrhythmia. Overexpression of miR-1 significantly inhibited both endogenous Cx43 expression and Cx43 3′UTR luciferase activity in HL-1 cells. Mutation of census binding site of +1586–1593 bp not +465–472 bp in Cx43 3′UTR luciferase resulted in abolishment of miR-1 inhibitory effects in HL-1 cells. Loss-of- function of miR-1 restored CVB3-induced Cx43 expression reduction in cultured HL-1 cells. The presence of ARDE attenuated the augmented miR-1 induced by CVB3 infection in vivo and in vitro. Conclusion This study identified that CVB3 infection reduced Cx43 expression by elevating miR-1 level in mouse viral myocarditis. For the first time, ARDE was shown to prevent arrhythmia, and rescue CVB3-induced endogenous Cx43 expression by regulating miR-1 level.
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Astragalus Root dry extract restores connexin43 expression by targeting miR-1 in viral myocarditis.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2018Co-Authors: Yu Wang, Li-ying Xuan, Yongfeng Liu, Liqun Shao, Cheng-xi Wei, Ming ZhaoAbstract:Viral myocarditis is defined as viral infection of myocardial tissue leading to impaired heart function and heart failure. Accumulating evidences have shown that arrhythmia is one of important complicating diseases of viral myocarditis causing increased mortality and morbidity. There are no effective treatment for the viral infection and complicating arrhythmia. This study investigated the effect and mechanism of Astragalus Root dry extract (ARDE) on arrhythmia induced by CVB3 in mice. The mice and HL-1 cells were treated with CVB3 and ARDE. Reciprocal regulation of Cx43 and miR-1 were observed in the CVB3 infected mouse myocardium and culture HL-1 cells. CVB3 IP injection increased immune cell infiltration in mouse left ventricle and caused irregular arrhythmia. ARDE treatment prevented the increase of immune cell infiltration and arrhythmia. Overexpression of miR-1 significantly inhibited both endogenous Cx43 expression and Cx43 3'UTR luciferase activity in HL-1 cells. Mutation of census binding site of +1586-1593 bp not +465-472 bp in Cx43 3'UTR luciferase resulted in abolishment of miR-1 inhibitory effects in HL-1 cells. Loss-of- function of miR-1 restored CVB3-induced Cx43 expression reduction in cultured HL-1 cells. The presence of ARDE attenuated the augmented miR-1 induced by CVB3 infection in vivo and in vitro. This study identified that CVB3 infection reduced Cx43 expression by elevating miR-1 level in mouse viral myocarditis. For the first time, ARDE was shown to prevent arrhythmia, and rescue CVB3-induced endogenous Cx43 expression by regulating miR-1 level. Copyright © 2018 Elsevier GmbH. All rights reserved.
Youngae Jung - One of the best experts on this subject based on the ideXlab platform.
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using 1H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the 1H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of 1H NMR and UPLC-MS analyses can be used to eval...
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Assessment of Peeling of Astragalus Roots Using 1H NMR- and UPLC-MS-Based Metabolite Profiling
Journal of agricultural and food chemistry, 2013Co-Authors: Jeeyoun Jung, Youngae Jung, Jin-sup Kim, Hyun Ryu, Geum-sook HwangAbstract:A metabolomic analysis was performed to examine the postharvest processing of Astragalus membranaceus Roots with a focus on the peeling procedure using (1)H NMR and UPLC-MS analyses. Principal component analysis (PCA) score plots from the (1)H NMR and UPLC-MS data showed clear separation between peeled and unpeeled Astragalus Roots. Peeled Roots exhibited significant losses of several primary metabolites, including acetate, alanine, arginine, caprate, fumarate, glutamate, histidine, N-acetylaspartate, malate, proline, sucrose, trigonelline, and valine. In contrast, the peeled Roots contained higher levels of asparagine, aspartate, and xylose, which are xylem-related compounds, and formate, which is produced in response to wound stress incurred during postharvest processing. In addition, the levels of isoflavonoids and astragalosides were significantly reduced in peeled Astragalus Root. These results demonstrate that metabolite profiling based on a combination of (1)H NMR and UPLC-MS analyses can be used to evaluate peeling procedures used in the postharvest processing of herbal medicines.
Yu Wang - One of the best experts on this subject based on the ideXlab platform.
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Astragalus Root dry extract restores connexin43 expression by targeting mir 1 in viral myocarditis
Phytomedicine, 2018Co-Authors: Yu Wang, Li-ying Xuan, Yongfeng Liu, Liqun Shao, Cheng-xi Wei, Ming ZhaoAbstract:Abstract Background Viral myocarditis is defined as viral infection of myocardial tissue leading to impaired heart function and heart failure. Accumulating evidences have shown that arrhythmia is one of important complicating diseases of viral myocarditis causing increased mortality and morbidity. There are no effective treatment for the viral infection and complicating arrhythmia. Purpose This study investigated the effect and mechanism of Astragalus Root dry extract (ARDE) on arrhythmia induced by CVB3 in mice. Methods The mice and HL-1 cells were treated with CVB3 and ARDE. Reciprocal regulation of Cx43 and miR-1 were observed in the CVB3 infected mouse myocardium and culture HL-1 cells. Results CVB3 IP injection increased immune cell infiltration in mouse left ventricle and caused irregular arrhythmia. ARDE treatment prevented the increase of immune cell infiltration and arrhythmia. Overexpression of miR-1 significantly inhibited both endogenous Cx43 expression and Cx43 3′UTR luciferase activity in HL-1 cells. Mutation of census binding site of +1586–1593 bp not +465–472 bp in Cx43 3′UTR luciferase resulted in abolishment of miR-1 inhibitory effects in HL-1 cells. Loss-of- function of miR-1 restored CVB3-induced Cx43 expression reduction in cultured HL-1 cells. The presence of ARDE attenuated the augmented miR-1 induced by CVB3 infection in vivo and in vitro. Conclusion This study identified that CVB3 infection reduced Cx43 expression by elevating miR-1 level in mouse viral myocarditis. For the first time, ARDE was shown to prevent arrhythmia, and rescue CVB3-induced endogenous Cx43 expression by regulating miR-1 level.
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Astragalus Root dry extract restores connexin43 expression by targeting miR-1 in viral myocarditis.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 2018Co-Authors: Yu Wang, Li-ying Xuan, Yongfeng Liu, Liqun Shao, Cheng-xi Wei, Ming ZhaoAbstract:Viral myocarditis is defined as viral infection of myocardial tissue leading to impaired heart function and heart failure. Accumulating evidences have shown that arrhythmia is one of important complicating diseases of viral myocarditis causing increased mortality and morbidity. There are no effective treatment for the viral infection and complicating arrhythmia. This study investigated the effect and mechanism of Astragalus Root dry extract (ARDE) on arrhythmia induced by CVB3 in mice. The mice and HL-1 cells were treated with CVB3 and ARDE. Reciprocal regulation of Cx43 and miR-1 were observed in the CVB3 infected mouse myocardium and culture HL-1 cells. CVB3 IP injection increased immune cell infiltration in mouse left ventricle and caused irregular arrhythmia. ARDE treatment prevented the increase of immune cell infiltration and arrhythmia. Overexpression of miR-1 significantly inhibited both endogenous Cx43 expression and Cx43 3'UTR luciferase activity in HL-1 cells. Mutation of census binding site of +1586-1593 bp not +465-472 bp in Cx43 3'UTR luciferase resulted in abolishment of miR-1 inhibitory effects in HL-1 cells. Loss-of- function of miR-1 restored CVB3-induced Cx43 expression reduction in cultured HL-1 cells. The presence of ARDE attenuated the augmented miR-1 induced by CVB3 infection in vivo and in vitro. This study identified that CVB3 infection reduced Cx43 expression by elevating miR-1 level in mouse viral myocarditis. For the first time, ARDE was shown to prevent arrhythmia, and rescue CVB3-induced endogenous Cx43 expression by regulating miR-1 level. Copyright © 2018 Elsevier GmbH. All rights reserved.
