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Maria P Portillo - One of the best experts on this subject based on the ideXlab platform.

  • effects of trans 10 cis 12 conjugated linoleic acid on the expression of uncoupling proteins in hamsters fed an Atherogenic Diet
    British Journal of Nutrition, 2007
    Co-Authors: Joan Ribot, Maria P Portillo, Catalina Pico, Teresa M Macarulla, Andreu Palou
    Abstract:

    It is known that conjugated linoleic acid (CLA) feeding decreases body adiposity but the mechanisms involved are not clear. The aim of this studywas to analyse whether alterations in uncoupling protein (UCP) expression in white and brown adipose tissues (WAT and BAT, respectively) andin skeletal muscle may be responsible for the effect of trans-10, cis-12 CLA on the size of body fat depots in hamsters. Animals were divided intothree groups and fed an Atherogenic Diet with different amounts of trans-10, cis-12 CLA (0 control, 0·5, or 1g/100g Diet) for 6 weeks. CLA feedingreduced adipose depot weights, but had no effect on body weight. Leptin mRNA expression decreased in both subcutaneous and perirenal WATdepots, in accordance with lower adiposity, whereas resistin mRNA expression was not changed. Animals fed CLA had lower UCP1 mRNA levelsin BAT (both doses of CLA) and in perirenal WAT (the low dose), and lower UCP3 mRNA levels in subcutaneous WAT (the high dose). UCP2mRNA expression in WAT was not significantly affected by CLA feeding. Animals fed the high dose of CLA showed increased UCP3 and car-nitine palmitoyl transferase-I (CPT-I) mRNA expression levels in skeletal muscle. In summary, induction of UCP1 or UCP2 in WAT and BAT isnot likely to be responsible for the fat-reduction action of CLA, but the increased expression of UCP3 in skeletal muscle, together with a higherexpression of CPT-I, may explain the previously reported effects of Dietary CLA in lowering adiposity and increasing fatty acid oxidation by skel-etal muscle.Conjugated linoleic acid: Uncoupling proteins: Atherogenic Diet: Hamster

  • trans 10 cis 12 conjugated linoleic acid prevents adiposity but not insulin resistance induced by an Atherogenic Diet in hamsters
    Journal of Nutritional Biochemistry, 2006
    Co-Authors: Edurne Simon, Teresa M Macarulla, Itziar Churruca, Alfredo Fernandezquintela, Maria P Portillo
    Abstract:

    Abstract While conjugated linoleic acid (CLA) has received a great deal of attention as a supplement that can favourably modify body composition, its potential impact on insulin sensitivity has not received equal attention. The aim of the present work was to analyse the effects of trans -10, cis -12 CLA isomer on insulin sensitivity in hamsters fed an Atherogenic Diet. Hamsters were divided into three groups: one group was fed a chow Diet (control) and the other two a semipurified Atherogenic Diet supplemented with 0.5% linoleic acid (LA) or trans -10, cis -12 CLA, respectively. Serum glucose, FFAs, insulin, leptin and adiponectin were measured using commercial kits. HOMA-IR was calculated using the formula of Matthews et al. PPARγ mRNA was assessed in epididymal adipose tissue by reverse transcription–polymerase chain reaction (RT-PCR). After 6 weeks, Atherogenic feeding produced an increase in body fat accumulation as compared with control feeding. The addition of trans -10, cis -12 CLA to the Atherogenic Diet avoided this feature. Atherogenic feeding also led to significantly higher serum concentrations of glucose, insulin, FFAs, as well as greater HOMA-IR values. trans -10, cis -12 CLA did not prevent these effects. No significant differences were found among experimental groups in serum leptin and adiponectin concentrations, nor in PPARγ expression. In summary, although the addition of trans -10, cis -12 CLA to an Atherogenic Diet reduces fat accumulation, it does not improve the impairment of insulin action associated with this feeding. The maintenance of insulin resistance in hamsters fed the Atherogenic CLA-enriched Diet is probably due to the high serum FFA concentration observed in these animals.

  • effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet
    Journal of Physiology and Biochemistry, 2003
    Co-Authors: Virginia Navarro, Edurne Simon, Alfredo Fernandezquintela, Amaia Zabala, M T Macarulla, Victor M Rodriguez, Maria P Portillo
    Abstract:

    Conjugated linoleic acid (CLA) refers to a mixture of naturally occurring positional and geometric isomers of linoleic acid that exist in dairy products and meat. The aim of the present work was to study the effects ofc-9,t-11 andt-10,c-12 CLA isomers on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet. Hamsters were divided in four groups: one group was fed a chow Diet (control) and the other three groups were given semi-purified Atherogenic Diets with 0.5% linoleic acid (LA),c-9,t-11 ort-10,c-12 CLA. Body weight and food intake were measured daily. After 6 weeks, adipose tissues from different anatomical locations and liver were dissected and weighed. Serum glucose, total cholesterol, HDL-c, LDL-c and triacylglycerol levels, as well as total and free cholesterol, triacylglycerol and phospholipid content in liver were determined by enzymatic methods. No differences in either energy intake or final body weight were found. The addition oft-10,c-12 CLA reduced fat accumulation and led to lower serum cholesterol, as compared with LA group. Nevertheless the level remained higher than in the control animals. The reduction in serum cholesterol was limited to LDL-c. This isomer also reduced triacylglycerol content in liver but did not modify serum triacylglycerol level. In summary, the present study demonstrates thatt-10,c-12 CLA is the biologically active agent when anti-obesity and hypocholesterolaemic properties of CLA are considered. In contrast, the isomerc-9,t-11 has no effect on lipid metabolism in hamsters.

Edurne Simon - One of the best experts on this subject based on the ideXlab platform.

  • trans 10 cis 12 conjugated linoleic acid prevents adiposity but not insulin resistance induced by an Atherogenic Diet in hamsters
    Journal of Nutritional Biochemistry, 2006
    Co-Authors: Edurne Simon, Teresa M Macarulla, Itziar Churruca, Alfredo Fernandezquintela, Maria P Portillo
    Abstract:

    Abstract While conjugated linoleic acid (CLA) has received a great deal of attention as a supplement that can favourably modify body composition, its potential impact on insulin sensitivity has not received equal attention. The aim of the present work was to analyse the effects of trans -10, cis -12 CLA isomer on insulin sensitivity in hamsters fed an Atherogenic Diet. Hamsters were divided into three groups: one group was fed a chow Diet (control) and the other two a semipurified Atherogenic Diet supplemented with 0.5% linoleic acid (LA) or trans -10, cis -12 CLA, respectively. Serum glucose, FFAs, insulin, leptin and adiponectin were measured using commercial kits. HOMA-IR was calculated using the formula of Matthews et al. PPARγ mRNA was assessed in epididymal adipose tissue by reverse transcription–polymerase chain reaction (RT-PCR). After 6 weeks, Atherogenic feeding produced an increase in body fat accumulation as compared with control feeding. The addition of trans -10, cis -12 CLA to the Atherogenic Diet avoided this feature. Atherogenic feeding also led to significantly higher serum concentrations of glucose, insulin, FFAs, as well as greater HOMA-IR values. trans -10, cis -12 CLA did not prevent these effects. No significant differences were found among experimental groups in serum leptin and adiponectin concentrations, nor in PPARγ expression. In summary, although the addition of trans -10, cis -12 CLA to an Atherogenic Diet reduces fat accumulation, it does not improve the impairment of insulin action associated with this feeding. The maintenance of insulin resistance in hamsters fed the Atherogenic CLA-enriched Diet is probably due to the high serum FFA concentration observed in these animals.

  • effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet
    Journal of Physiology and Biochemistry, 2003
    Co-Authors: Virginia Navarro, Edurne Simon, Alfredo Fernandezquintela, Amaia Zabala, M T Macarulla, Victor M Rodriguez, Maria P Portillo
    Abstract:

    Conjugated linoleic acid (CLA) refers to a mixture of naturally occurring positional and geometric isomers of linoleic acid that exist in dairy products and meat. The aim of the present work was to study the effects ofc-9,t-11 andt-10,c-12 CLA isomers on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet. Hamsters were divided in four groups: one group was fed a chow Diet (control) and the other three groups were given semi-purified Atherogenic Diets with 0.5% linoleic acid (LA),c-9,t-11 ort-10,c-12 CLA. Body weight and food intake were measured daily. After 6 weeks, adipose tissues from different anatomical locations and liver were dissected and weighed. Serum glucose, total cholesterol, HDL-c, LDL-c and triacylglycerol levels, as well as total and free cholesterol, triacylglycerol and phospholipid content in liver were determined by enzymatic methods. No differences in either energy intake or final body weight were found. The addition oft-10,c-12 CLA reduced fat accumulation and led to lower serum cholesterol, as compared with LA group. Nevertheless the level remained higher than in the control animals. The reduction in serum cholesterol was limited to LDL-c. This isomer also reduced triacylglycerol content in liver but did not modify serum triacylglycerol level. In summary, the present study demonstrates thatt-10,c-12 CLA is the biologically active agent when anti-obesity and hypocholesterolaemic properties of CLA are considered. In contrast, the isomerc-9,t-11 has no effect on lipid metabolism in hamsters.

Teresa M Macarulla - One of the best experts on this subject based on the ideXlab platform.

  • effects of trans 10 cis 12 conjugated linoleic acid on the expression of uncoupling proteins in hamsters fed an Atherogenic Diet
    British Journal of Nutrition, 2007
    Co-Authors: Joan Ribot, Maria P Portillo, Catalina Pico, Teresa M Macarulla, Andreu Palou
    Abstract:

    It is known that conjugated linoleic acid (CLA) feeding decreases body adiposity but the mechanisms involved are not clear. The aim of this studywas to analyse whether alterations in uncoupling protein (UCP) expression in white and brown adipose tissues (WAT and BAT, respectively) andin skeletal muscle may be responsible for the effect of trans-10, cis-12 CLA on the size of body fat depots in hamsters. Animals were divided intothree groups and fed an Atherogenic Diet with different amounts of trans-10, cis-12 CLA (0 control, 0·5, or 1g/100g Diet) for 6 weeks. CLA feedingreduced adipose depot weights, but had no effect on body weight. Leptin mRNA expression decreased in both subcutaneous and perirenal WATdepots, in accordance with lower adiposity, whereas resistin mRNA expression was not changed. Animals fed CLA had lower UCP1 mRNA levelsin BAT (both doses of CLA) and in perirenal WAT (the low dose), and lower UCP3 mRNA levels in subcutaneous WAT (the high dose). UCP2mRNA expression in WAT was not significantly affected by CLA feeding. Animals fed the high dose of CLA showed increased UCP3 and car-nitine palmitoyl transferase-I (CPT-I) mRNA expression levels in skeletal muscle. In summary, induction of UCP1 or UCP2 in WAT and BAT isnot likely to be responsible for the fat-reduction action of CLA, but the increased expression of UCP3 in skeletal muscle, together with a higherexpression of CPT-I, may explain the previously reported effects of Dietary CLA in lowering adiposity and increasing fatty acid oxidation by skel-etal muscle.Conjugated linoleic acid: Uncoupling proteins: Atherogenic Diet: Hamster

  • trans 10 cis 12 conjugated linoleic acid prevents adiposity but not insulin resistance induced by an Atherogenic Diet in hamsters
    Journal of Nutritional Biochemistry, 2006
    Co-Authors: Edurne Simon, Teresa M Macarulla, Itziar Churruca, Alfredo Fernandezquintela, Maria P Portillo
    Abstract:

    Abstract While conjugated linoleic acid (CLA) has received a great deal of attention as a supplement that can favourably modify body composition, its potential impact on insulin sensitivity has not received equal attention. The aim of the present work was to analyse the effects of trans -10, cis -12 CLA isomer on insulin sensitivity in hamsters fed an Atherogenic Diet. Hamsters were divided into three groups: one group was fed a chow Diet (control) and the other two a semipurified Atherogenic Diet supplemented with 0.5% linoleic acid (LA) or trans -10, cis -12 CLA, respectively. Serum glucose, FFAs, insulin, leptin and adiponectin were measured using commercial kits. HOMA-IR was calculated using the formula of Matthews et al. PPARγ mRNA was assessed in epididymal adipose tissue by reverse transcription–polymerase chain reaction (RT-PCR). After 6 weeks, Atherogenic feeding produced an increase in body fat accumulation as compared with control feeding. The addition of trans -10, cis -12 CLA to the Atherogenic Diet avoided this feature. Atherogenic feeding also led to significantly higher serum concentrations of glucose, insulin, FFAs, as well as greater HOMA-IR values. trans -10, cis -12 CLA did not prevent these effects. No significant differences were found among experimental groups in serum leptin and adiponectin concentrations, nor in PPARγ expression. In summary, although the addition of trans -10, cis -12 CLA to an Atherogenic Diet reduces fat accumulation, it does not improve the impairment of insulin action associated with this feeding. The maintenance of insulin resistance in hamsters fed the Atherogenic CLA-enriched Diet is probably due to the high serum FFA concentration observed in these animals.

Andreu Palou - One of the best experts on this subject based on the ideXlab platform.

  • effects of trans 10 cis 12 conjugated linoleic acid on the expression of uncoupling proteins in hamsters fed an Atherogenic Diet
    British Journal of Nutrition, 2007
    Co-Authors: Joan Ribot, Maria P Portillo, Catalina Pico, Teresa M Macarulla, Andreu Palou
    Abstract:

    It is known that conjugated linoleic acid (CLA) feeding decreases body adiposity but the mechanisms involved are not clear. The aim of this studywas to analyse whether alterations in uncoupling protein (UCP) expression in white and brown adipose tissues (WAT and BAT, respectively) andin skeletal muscle may be responsible for the effect of trans-10, cis-12 CLA on the size of body fat depots in hamsters. Animals were divided intothree groups and fed an Atherogenic Diet with different amounts of trans-10, cis-12 CLA (0 control, 0·5, or 1g/100g Diet) for 6 weeks. CLA feedingreduced adipose depot weights, but had no effect on body weight. Leptin mRNA expression decreased in both subcutaneous and perirenal WATdepots, in accordance with lower adiposity, whereas resistin mRNA expression was not changed. Animals fed CLA had lower UCP1 mRNA levelsin BAT (both doses of CLA) and in perirenal WAT (the low dose), and lower UCP3 mRNA levels in subcutaneous WAT (the high dose). UCP2mRNA expression in WAT was not significantly affected by CLA feeding. Animals fed the high dose of CLA showed increased UCP3 and car-nitine palmitoyl transferase-I (CPT-I) mRNA expression levels in skeletal muscle. In summary, induction of UCP1 or UCP2 in WAT and BAT isnot likely to be responsible for the fat-reduction action of CLA, but the increased expression of UCP3 in skeletal muscle, together with a higherexpression of CPT-I, may explain the previously reported effects of Dietary CLA in lowering adiposity and increasing fatty acid oxidation by skel-etal muscle.Conjugated linoleic acid: Uncoupling proteins: Atherogenic Diet: Hamster

Alfredo Fernandezquintela - One of the best experts on this subject based on the ideXlab platform.

  • trans 10 cis 12 conjugated linoleic acid prevents adiposity but not insulin resistance induced by an Atherogenic Diet in hamsters
    Journal of Nutritional Biochemistry, 2006
    Co-Authors: Edurne Simon, Teresa M Macarulla, Itziar Churruca, Alfredo Fernandezquintela, Maria P Portillo
    Abstract:

    Abstract While conjugated linoleic acid (CLA) has received a great deal of attention as a supplement that can favourably modify body composition, its potential impact on insulin sensitivity has not received equal attention. The aim of the present work was to analyse the effects of trans -10, cis -12 CLA isomer on insulin sensitivity in hamsters fed an Atherogenic Diet. Hamsters were divided into three groups: one group was fed a chow Diet (control) and the other two a semipurified Atherogenic Diet supplemented with 0.5% linoleic acid (LA) or trans -10, cis -12 CLA, respectively. Serum glucose, FFAs, insulin, leptin and adiponectin were measured using commercial kits. HOMA-IR was calculated using the formula of Matthews et al. PPARγ mRNA was assessed in epididymal adipose tissue by reverse transcription–polymerase chain reaction (RT-PCR). After 6 weeks, Atherogenic feeding produced an increase in body fat accumulation as compared with control feeding. The addition of trans -10, cis -12 CLA to the Atherogenic Diet avoided this feature. Atherogenic feeding also led to significantly higher serum concentrations of glucose, insulin, FFAs, as well as greater HOMA-IR values. trans -10, cis -12 CLA did not prevent these effects. No significant differences were found among experimental groups in serum leptin and adiponectin concentrations, nor in PPARγ expression. In summary, although the addition of trans -10, cis -12 CLA to an Atherogenic Diet reduces fat accumulation, it does not improve the impairment of insulin action associated with this feeding. The maintenance of insulin resistance in hamsters fed the Atherogenic CLA-enriched Diet is probably due to the high serum FFA concentration observed in these animals.

  • effects of conjugated linoleic acid on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet
    Journal of Physiology and Biochemistry, 2003
    Co-Authors: Virginia Navarro, Edurne Simon, Alfredo Fernandezquintela, Amaia Zabala, M T Macarulla, Victor M Rodriguez, Maria P Portillo
    Abstract:

    Conjugated linoleic acid (CLA) refers to a mixture of naturally occurring positional and geometric isomers of linoleic acid that exist in dairy products and meat. The aim of the present work was to study the effects ofc-9,t-11 andt-10,c-12 CLA isomers on body fat accumulation and serum lipids in hamsters fed an Atherogenic Diet. Hamsters were divided in four groups: one group was fed a chow Diet (control) and the other three groups were given semi-purified Atherogenic Diets with 0.5% linoleic acid (LA),c-9,t-11 ort-10,c-12 CLA. Body weight and food intake were measured daily. After 6 weeks, adipose tissues from different anatomical locations and liver were dissected and weighed. Serum glucose, total cholesterol, HDL-c, LDL-c and triacylglycerol levels, as well as total and free cholesterol, triacylglycerol and phospholipid content in liver were determined by enzymatic methods. No differences in either energy intake or final body weight were found. The addition oft-10,c-12 CLA reduced fat accumulation and led to lower serum cholesterol, as compared with LA group. Nevertheless the level remained higher than in the control animals. The reduction in serum cholesterol was limited to LDL-c. This isomer also reduced triacylglycerol content in liver but did not modify serum triacylglycerol level. In summary, the present study demonstrates thatt-10,c-12 CLA is the biologically active agent when anti-obesity and hypocholesterolaemic properties of CLA are considered. In contrast, the isomerc-9,t-11 has no effect on lipid metabolism in hamsters.