Autoantibody

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Markku Maki - One of the best experts on this subject based on the ideXlab platform.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328

Ilma Rita Korponayszabo - One of the best experts on this subject based on the ideXlab platform.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328

S Sulkanen - One of the best experts on this subject based on the ideXlab platform.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328

Annikki Sarnesto - One of the best experts on this subject based on the ideXlab platform.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328

Pekka Collin - One of the best experts on this subject based on the ideXlab platform.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.

  • tissue transglutaminase Autoantibody enzyme linked immunosorbent assay in detecting celiac disease
    Gastroenterology, 1998
    Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku Maki
    Abstract:

    Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase Autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new Autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase Autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase Autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328

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