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Hiroki Nishida - One of the best experts on this subject based on the ideXlab platform.
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FGF9/16/20 and Wnt-5α signals are involved in specification of secondary muscle fate in embryos of the ascidian, Halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi . We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis , was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
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fgf9 16 20 and wnt 5α signals are involved in specification of secondary muscle fate in embryos of the ascidian halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi. We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis, was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
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Suppression of Muscle Fate by Cellular Interaction Is Required for Mesenchyme Formation during Ascidian Embryogenesis
Developmental Biology, 1999Co-Authors: Gil Jung Kim, Hiroki NishidaAbstract:Abstract The tadpole larva of the ascidian Halocynthia roretzi has several hundred mesenchyme cells in its trunk. Mesenchyme cells are exclusively derived from the B8.5 and B7.7 blastomere pairs of the 110-cell embryo. It has been believed that specification of mesenchyme cells is an Autonomous Process. In the present study, we have demonstrated that presumptive-mesenchyme blastomeres isolated from early 32-cell embryos did not express mesenchyme-specific features, whereas those isolated after the late 64-cell stage developed mesenchyme markers Autonomously. Results of experiments involving coisolation and recombination of blastomeres showed that cellular interaction with adjacent presumptive-endoderm blastomeres during the late 32- and early 64-cell stages is required for mesenchyme formation. When such interaction was absent, the presumptive-mesenchyme blastomeres developed into muscle cells. Therefore, a signal from endoderm precursor blastomeres promotes mesenchyme fate, suppressing the muscle fate that is specified by ooplasmic muscle determinants. In Halocynthia, the muscle actin gene was precociously activated in mesenchyme–muscle precursor blastomeres at the 32-cell stage, and the mesenchyme and muscle fates were separated into two daughter blastomeres at the next cleavage. In presumptive-mesenchyme blastomeres at the 64-cell stage, expression of the muscle actin gene was immediately down-regulated by the signal from the neighboring endoderm precursor blastomeres. Thus, mesenchyme formation involves a novel mechanism of fate specification in ascidians, where formation of mesenchyme cells requires cellular interaction that suppresses muscle fate in the mesenchyme precursor blastomeres.
Miki Tokuoka - One of the best experts on this subject based on the ideXlab platform.
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FGF9/16/20 and Wnt-5α signals are involved in specification of secondary muscle fate in embryos of the ascidian, Halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi . We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis , was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
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fgf9 16 20 and wnt 5α signals are involved in specification of secondary muscle fate in embryos of the ascidian halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi. We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis, was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
Gaku Kumano - One of the best experts on this subject based on the ideXlab platform.
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FGF9/16/20 and Wnt-5α signals are involved in specification of secondary muscle fate in embryos of the ascidian, Halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi . We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis , was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
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fgf9 16 20 and wnt 5α signals are involved in specification of secondary muscle fate in embryos of the ascidian halocynthia roretzi
Development Genes and Evolution, 2007Co-Authors: Miki Tokuoka, Gaku Kumano, Hiroki NishidaAbstract:The tail muscle cells of the ascidian tadpole larva originate from two different lineages, the B- (primary) and A- and b- (secondary) line blastomeres of the eight-cell stage embryo. The primary muscle cells assume muscle fate cell-Autonomously with the involvement of a localized muscle determinant, macho-1. On the other hand, fate determination of secondary muscle cells is a non-cell-Autonomous Process that depends on cellular interactions. In this paper, we investigated the mechanisms underlying fate specification of secondary muscle cells in Halocynthia roretzi. We found that FGF and Wnt5 signals were required. In contrast, the Nodal signal, which is required for specification of A-line muscle cells in another ascidian, Ciona intestinalis, was not necessary for the formation of any secondary muscle cells in Halocynthia embryo. Therefore, Halocynthia and Ciona show distinctly different mechanisms for generation of the secondary lineages, despite the fact that embryogenesis appears very similar between these species. We also found that the mechanisms involved in specification of A- and b-line muscle cells were distinct in that the required timing of the FGF signal for the A-line muscle cells preceded that for the b-line. Moreover, the inducer blastomeres for specification of these two lineages were different.
Ghalib A. Al-hashim - One of the best experts on this subject based on the ideXlab platform.
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Novel Design of Heterogeneous Automation Controller Based on Real-Time Data Distribution Service Middleware to Avoid Obsolescence Challenges
Journal of Circuits Systems and Computers, 2016Co-Authors: Sadiq M. Sait, Ghalib A. Al-hashimAbstract:Oil and gas Processing facilities utilize various Process automation systems with proprietary controllers. As the systems age; older technologies become obsolete resulting in frequent premature capital investments to sustain their operation. This paper presents a new design of automation controller to provide inherent mechanisms for upgrades and/or partial replacement of any obsolete components without obligation for a complete system replacement throughout the expected life cycle of the Processing facilities. The input/output racks are physically and logically decoupled from the controller by converting them into distributed Autonomous Process interface systems. The proprietary input/output communication between the conventional controller CPU and the associated input/output racks is replaced with standard real-time data distribution service middleware for providing seamless cross-vendor interoperable communication between the controller and the distributed Autonomous Process interface systems. The objec...
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Novel Design of Collaborative Automation Platform Using Real-Time Data Distribution Service Middleware for an Optimum Process Control Environment
Journal of Circuits Systems and Computers, 2016Co-Authors: Sadiq M. Sait, Ghalib A. Al-hashimAbstract:Refining and petrochemical Processing facilities utilize various Process control applications to raise productivity and enhance plant operation. Client–server communication model is used for integrating these highly interacting applications across multiple network layers utilized in distributed control systems. This paper presents an optimum Process control environment by merging sequential and regulatory control, advanced regulatory control, multivariable control, unit-based Process control, and plant-wide advanced Process control into a single collaborative automation platform to ensure optimum operation of Processing equipment for achieving maximum yield of all manufacturing facilities. The main control module is replaced by a standard real-time server. The input/output racks are physically and logically decoupled from the controller by converting them into distributed Autonomous Process interface systems. Real-time data distribution service middleware is used for providing seamless cross-vendor interoperable communication among all Process control applications and distributed Autonomous Process interface systems. Detailed performance analysis was conducted to evaluate the average communication latency and aggregate messaging capacity among Process control applications and distributed Autonomous Process interface systems. The overall performance results confirm the viability of the new proposal as the basis for designing an optimal collaborative automation platform to handle all Process control applications. It also does not impose any inherent limit on the aggregate data messaging capacity, making it suitable for scalable automation platforms.
Baldin Elisa - One of the best experts on this subject based on the ideXlab platform.
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Rhythm and continuity in the intervention to reactivate vacant urban spaces. Recognize the stratification of places to engage an Autonomous Process of integration between the parts.
2020Co-Authors: Caravello Chiara, Baldin ElisaAbstract:peer reviewedaudience: researcher, professional, student, popularizationssociating the architectural intervention of recovery - required to respond to urgent, necessarily impatient social questions - to the work on the landscape - educated to slowness and natural rhythms - pushes us to contemplate the aleatory, the sense of uncertainty, imposing the respect for places and materials as much as for times, at the basis of a constantly evolving project. The landscape, as an "upstream condition" of human and natural actions (ELC,2000), requires recognition and intervention strategies with very high standards of flexibility, sized on relatively low financial resources and capable of covering an ever-widening multiplicity of needs, linked to a variety of identities that are now living together, with consequent need of integration, on the same territory. A vision must be retraced: to observe the landscape as the logical linking of places and signifiers, a stratification Process already in progress as it is constantly changing (Besse, 2009). With this in mind, the adoption of an infiltration strategy, the grafting of an evolutionary new transformation Process, is proposed. Through the study of a post-industrial recovery intervention case, located in the region of North Rhine-Westphalia in Germany, the value of defining a long-term intervention program capable of removing in the first instance the idea of degradation around the area, is recognized (Masboungi, 2002). It is a matter of engaging non-characterizing and non-limiting strategies, based on the non-definitive sense: a time of experimentation, the suggestion of reuse and regeneration-in-progress hypotheses. The intervention develops by means of action, adopting a mélange of techniques as a complex tool for gradual resolution, with an increasing economic capacity, thanks to targeted investments and reduced timing. The identification of a new function, capable of improving the value of the existent by revealing the hidden potential, being useful for its purpose and lasting over time, is therefore associated with the grafting of a design seed, an action on an indefinite time
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Rhythm and continuity in the intervention to reactivate vacant urban spaces. Recognize the stratification of places to engage an Autonomous Process of integration between the parts.
2020Co-Authors: Caravello Chiara, Baldin ElisaAbstract:ssociating the architectural intervention of recovery - required to respond to urgent, necessarily impatient social questions - to the work on the landscape - educated to slowness and natural rhythms - pushes us to contemplate the aleatory, the sense of uncertainty, imposing the respect for places and materials as much as for times, at the basis of a constantly evolving project. The landscape, as an "upstream condition" of human and natural actions (ELC,2000), requires recognition and intervention strategies with very high standards of flexibility, sized on relatively low financial resources and capable of covering an ever-widening multiplicity of needs, linked to a variety of identities that are now living together, with consequent need of integration, on the same territory. A vision must be retraced: to observe the landscape as the logical linking of places and signifiers, a stratification Process already in progress as it is constantly changing (Besse, 2009). With this in mind, the adoption of an infiltration strategy, the grafting of an evolutionary new transformation Process, is proposed. Through the study of a post-industrial recovery intervention case, located in the region of North Rhine-Westphalia in Germany, the value of defining a long-term intervention program capable of removing in the first instance the idea of degradation around the area, is recognized (Masboungi, 2002). It is a matter of engaging non-characterizing and non-limiting strategies, based on the non-definitive sense: a time of experimentation, the suggestion of reuse and regeneration-in-progress hypotheses. The intervention develops by means of action, adopting a mélange of techniques as a complex tool for gradual resolution, with an increasing economic capacity, thanks to targeted investments and reduced timing. The identification of a new function, capable of improving the value of the existent by revealing the hidden potential, being useful for its purpose and lasting over time, is therefore associated with the grafting of a design seed, an action on an indefinite time.Peer reviewe
