The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform

Yuuhiko Tanabe - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the fast growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 Autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.

  • The fast-growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 Autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.

Nicole Dubilier - One of the best experts on this subject based on the ideXlab platform.

  • sulfur oxidizing symbionts without canonical genes for Autotrophic co2 fixation
    Mbio, 2019
    Co-Authors: Brandon K B Seah, Chakkiath Paul Antony, Jan Zarzycki, Lennart Schada Von Borzyskowski, Angela Kouris, Manuel Kleiner, Manuel Liebeke, Nicole Dubilier, Bruno Huettel
    Abstract:

    ABSTRACT Since the discovery of symbioses between sulfur-oxidizing (thiotrophic) bacteria and invertebrates at hydrothermal vents over 40 years ago, it has been assumed that Autotrophic fixation of CO2 by the symbionts drives these nutritional associations. In this study, we investigated “Candidatus Kentron,” the clade of symbionts hosted by Kentrophoros, a diverse genus of ciliates which are found in marine coastal sediments around the world. Despite being the main food source for their hosts, Kentron bacteria lack the key canonical genes for any of the known pathways for Autotrophic carbon fixation and have a carbon stable isotope fingerprint that is unlike other thiotrophic symbionts from similar habitats. Our genomic and transcriptomic analyses instead found metabolic features consistent with growth on organic carbon, especially organic and amino acids, for which they have abundant uptake transporters. All known thiotrophic symbionts have converged on using reduced sulfur to gain energy lithotrophically, but they are diverse in their carbon sources. Some clades are obligate Autotrophs, while many are mixotrophs that can supplement Autotrophic carbon fixation with heterotrophic capabilities similar to those in Kentron. Here we show that Kentron bacteria are the only thiotrophic symbionts that appear to be entirely heterotrophic, unlike all other thiotrophic symbionts studied to date, which possess either the Calvin-Benson-Bassham or the reverse tricarboxylic acid cycle for Autotrophy. IMPORTANCE Many animals and protists depend on symbiotic sulfur-oxidizing bacteria as their main food source. These bacteria use energy from oxidizing inorganic sulfur compounds to make biomass Autotrophically from CO2, serving as primary producers for their hosts. Here we describe a clade of nonAutotrophic sulfur-oxidizing symbionts, “Candidatus Kentron,” associated with marine ciliates. They lack genes for known Autotrophic pathways and have a carbon stable isotope fingerprint heavier than other symbionts from similar habitats. Instead, they have the potential to oxidize sulfur to fuel the uptake of organic compounds for heterotrophic growth, a metabolic mode called chemolithoheterotrophy that is not found in other symbioses. Although several symbionts have heterotrophic features to supplement primary production, in Kentron they appear to supplant it entirely.

  • sulfur oxidizing symbionts without canonical genes for Autotrophic co2 fixation
    bioRxiv, 2019
    Co-Authors: Brandon K B Seah, Chakkiath Paul Antony, Jan Zarzycki, Lennart Schada Von Borzyskowski, Angela Kouris, Manuel Kleiner, Manuel Liebeke, Nicole Dubilier, Bruno Huettel, Harald R Grubervodicka
    Abstract:

    Since the discovery of symbioses between sulfur-oxidizing (thiotrophic) bacteria and invertebrates at hydrothermal vents over 40 years ago, it has been assumed that Autotrophic fixation of CO2 by the symbionts drives these nutritional associations. In this study, we investigated Candidatus Kentron, the clade of symbionts hosted by Kentrophoros, a diverse genus of ciliates which are found in marine coastal sediments around the world. Despite being the main food source for their hosts, Kentron lack the key canonical genes for any of the known pathways for Autotrophic fixation, and have a carbon stable isotope fingerprint unlike other thiotrophic symbionts from similar habitats. Our genomic and transcriptomic analyses instead found metabolic features consistent with growth on organic carbon, especially organic and amino acids, for which they have abundant uptake transporters. All known thiotrophic symbionts have converged on using reduced sulfur to generate energy lithotrophically, but they are diverse in their carbon sources. Some clades are obligate Autotrophs, while many are mixotrophs that can supplement Autotrophic carbon fixation with heterotrophic capabilities similar to those in Kentron. We have shown that Kentron are the only thiotrophic symbionts that appear to be entirely heterotrophic, unlike all other thiotrophic symbionts studied to date, which possess either the Calvin-Benson-Bassham or reverse tricarboxylic acid cycles for Autotrophy.

Makoto Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the fast growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 Autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.

Makoto M Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • The fast-growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 Autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.

Motohide Ioki - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the fast growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 Autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.

  • The fast-growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 Autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the fast-growing strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 Autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 Autotroph.