The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Pei-yuan Qian - One of the best experts on this subject based on the ideXlab platform.
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effect of polybrominated diphenyl ether pbde treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Frontiers in Microbiology, 2015Co-Authors: Renmao Tian, Yong Wang, Pei-yuan Qian, Salim Bougouffa, Jill M Y Chiu, Rudolf S S WuAbstract:Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the Bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the Bacterial community shifted from an Autotrophic Bacteria-dominated community to a heterotrophic Bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 µg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic Bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic Bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and Bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated Bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the Bacterial community in the microenvironment of the sponge.
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effect of copper treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Mbio, 2014Co-Authors: Renmao Tian, Yong Wang, Salim Bougouffa, Weipeng Zhang, Vladimir B Bajic, Pei-yuan QianAbstract:Marine sponges are the most primitive metazoan and host symbiotic microorganisms. They are crucial components of the marine ecological system and play an essential role in pelagic processes. Copper pollution is currently a widespread problem and poses a threat to marine organisms. Here, we examined the effects of copper treatment on the composition of the sponge- associated Bacterial community and the genetic features that facilitate the survival of enriched Bacteria under copper stress. The 16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothi- orhodospiraceae and photosynthetic CyanoBacteriaas dominant species. However, these Autotrophic Bacteria decreased sub- stantially after treatment with a high copper concentration, which enriched for a heterotrophic-bacterium-dominated commu- nity. Metagenomic comparison revealed a varied profile of functional genes and enriched functions, including Bacterial motility and chemotaxis, extracellular polysaccharide and capsule synthesis, virulence-associated genes, and genes involved in cell signal- ing and regulation, suggesting short-period mechanisms of the enriched Bacterial community for surviving copper stress in the microenvironment of the sponge. Microscopic observation and comparison revealed dynamic Bacterial aggregation within the matrix and lysis of sponge cells. The bacteriophage community was also enriched, and the complete genome of a dominant phage was determined, implying that a lytic phage cycle was stimulated by the high copper concentration. This study demonstrated a copper-induced shift in the composition of functional genes of the sponge-associated Bacterial community, revealing the selec- tive effect of copper treatment on the functions of the Bacterial community in the microenvironment of the sponge. IMPORTANCE This study determined the Bacterial community structure of the common sponge Haliclona cymaeformis and ex- amined the effect of copper treatment on the community structure and functional gene composition, revealing that copper treat- ment had a selective effect on the functions of the Bacterial community in the sponge. Thesefindings suggest that copper pollu- tion has an ecological impact on the sponge symbiont. The analysis showed that the untreated sponges hosted symbiotic Autotrophic Bacteria as dominant species, and the high-concentration copper treatment enriched for a heterotrophic Bacterial community with enrichment for genes important for Bacterial motility, supplementary cellular components, signaling and regu- lation, and virulence. Microscopic observation showed obvious Bacterial aggregation and a reduction of sponge cell numbers in treated sponges, which suggested the formation of aggregates to reduce the copper concentration. The enrichment for functions of directional Bacterial movement and supplementary cellular components and the formation of Bacterial aggregates and phage enrichment are novelfindings in sponge studies.
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Microbial Sulfur Cycle in Two Hydrothermal Chimneys on the Southwest Indian Ridge
mBio, 2014Co-Authors: Huiluo Cao, Yong Wang, On On Lee, Xiang Zeng, Zongze Shao, Pei-yuan QianAbstract:Sulfur is an important element in sustaining microbial communities present in hydrothermal vents. Sulfur oxidation has been extensively studied due to its importance in chemosynthetic pathways in hydrothermal fields; however, less is known about sulfate reduction. Here, the metagenomes of hydrothermal chimneys located on the ultraslow-spreading Southwest Indian Ridge (SWIR) were pyrosequenced to elucidate the associated microbial sulfur cycle. A taxonomic summary of known genes revealed a few dominant Bacteria that participated in the microbial sulfur cycle, particularly sulfate-reducing DeltaproteoBacteria. The metagenomes studied contained highly abundant genes related to sulfur oxidation and reduction. Several carbon metabolic pathways, in particular the Calvin-Benson-Bassham pathway and the reductive tricarboxylic acid cycles for CO2 fixation, were identified in sulfur-oxidizing Autotrophic Bacteria. In contrast, highly abundant genes related to the oxidation of short-chain alkanes were grouped with sulfate-reducing Bacteria, suggesting an important role for short-chain alkanes in the sulfur cycle. Furthermore, sulfur-oxidizing Bacteria were associated with enrichment for genes involved in the denitrification pathway, while sulfate-reducing Bacteria displayed enrichment for genes responsible for hydrogen utilization. In conclusion, this study provides insights regarding major microbial metabolic activities that are driven by the sulfur cycle in low-temperature hydrothermal chimneys present on an ultraslow midocean ridge.
Yong Wang - One of the best experts on this subject based on the ideXlab platform.
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effect of polybrominated diphenyl ether pbde treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Frontiers in Microbiology, 2015Co-Authors: Renmao Tian, Yong Wang, Pei-yuan Qian, Salim Bougouffa, Jill M Y Chiu, Rudolf S S WuAbstract:Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the Bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the Bacterial community shifted from an Autotrophic Bacteria-dominated community to a heterotrophic Bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 µg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic Bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic Bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and Bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated Bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the Bacterial community in the microenvironment of the sponge.
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effect of copper treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Mbio, 2014Co-Authors: Renmao Tian, Yong Wang, Salim Bougouffa, Weipeng Zhang, Vladimir B Bajic, Pei-yuan QianAbstract:Marine sponges are the most primitive metazoan and host symbiotic microorganisms. They are crucial components of the marine ecological system and play an essential role in pelagic processes. Copper pollution is currently a widespread problem and poses a threat to marine organisms. Here, we examined the effects of copper treatment on the composition of the sponge- associated Bacterial community and the genetic features that facilitate the survival of enriched Bacteria under copper stress. The 16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothi- orhodospiraceae and photosynthetic CyanoBacteriaas dominant species. However, these Autotrophic Bacteria decreased sub- stantially after treatment with a high copper concentration, which enriched for a heterotrophic-bacterium-dominated commu- nity. Metagenomic comparison revealed a varied profile of functional genes and enriched functions, including Bacterial motility and chemotaxis, extracellular polysaccharide and capsule synthesis, virulence-associated genes, and genes involved in cell signal- ing and regulation, suggesting short-period mechanisms of the enriched Bacterial community for surviving copper stress in the microenvironment of the sponge. Microscopic observation and comparison revealed dynamic Bacterial aggregation within the matrix and lysis of sponge cells. The bacteriophage community was also enriched, and the complete genome of a dominant phage was determined, implying that a lytic phage cycle was stimulated by the high copper concentration. This study demonstrated a copper-induced shift in the composition of functional genes of the sponge-associated Bacterial community, revealing the selec- tive effect of copper treatment on the functions of the Bacterial community in the microenvironment of the sponge. IMPORTANCE This study determined the Bacterial community structure of the common sponge Haliclona cymaeformis and ex- amined the effect of copper treatment on the community structure and functional gene composition, revealing that copper treat- ment had a selective effect on the functions of the Bacterial community in the sponge. Thesefindings suggest that copper pollu- tion has an ecological impact on the sponge symbiont. The analysis showed that the untreated sponges hosted symbiotic Autotrophic Bacteria as dominant species, and the high-concentration copper treatment enriched for a heterotrophic Bacterial community with enrichment for genes important for Bacterial motility, supplementary cellular components, signaling and regu- lation, and virulence. Microscopic observation showed obvious Bacterial aggregation and a reduction of sponge cell numbers in treated sponges, which suggested the formation of aggregates to reduce the copper concentration. The enrichment for functions of directional Bacterial movement and supplementary cellular components and the formation of Bacterial aggregates and phage enrichment are novelfindings in sponge studies.
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Microbial Sulfur Cycle in Two Hydrothermal Chimneys on the Southwest Indian Ridge
mBio, 2014Co-Authors: Huiluo Cao, Yong Wang, On On Lee, Xiang Zeng, Zongze Shao, Pei-yuan QianAbstract:Sulfur is an important element in sustaining microbial communities present in hydrothermal vents. Sulfur oxidation has been extensively studied due to its importance in chemosynthetic pathways in hydrothermal fields; however, less is known about sulfate reduction. Here, the metagenomes of hydrothermal chimneys located on the ultraslow-spreading Southwest Indian Ridge (SWIR) were pyrosequenced to elucidate the associated microbial sulfur cycle. A taxonomic summary of known genes revealed a few dominant Bacteria that participated in the microbial sulfur cycle, particularly sulfate-reducing DeltaproteoBacteria. The metagenomes studied contained highly abundant genes related to sulfur oxidation and reduction. Several carbon metabolic pathways, in particular the Calvin-Benson-Bassham pathway and the reductive tricarboxylic acid cycles for CO2 fixation, were identified in sulfur-oxidizing Autotrophic Bacteria. In contrast, highly abundant genes related to the oxidation of short-chain alkanes were grouped with sulfate-reducing Bacteria, suggesting an important role for short-chain alkanes in the sulfur cycle. Furthermore, sulfur-oxidizing Bacteria were associated with enrichment for genes involved in the denitrification pathway, while sulfate-reducing Bacteria displayed enrichment for genes responsible for hydrogen utilization. In conclusion, this study provides insights regarding major microbial metabolic activities that are driven by the sulfur cycle in low-temperature hydrothermal chimneys present on an ultraslow midocean ridge.
Renmao Tian - One of the best experts on this subject based on the ideXlab platform.
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effect of polybrominated diphenyl ether pbde treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Frontiers in Microbiology, 2015Co-Authors: Renmao Tian, Yong Wang, Pei-yuan Qian, Salim Bougouffa, Jill M Y Chiu, Rudolf S S WuAbstract:Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the Bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the Bacterial community shifted from an Autotrophic Bacteria-dominated community to a heterotrophic Bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 µg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic Bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic Bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and Bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated Bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the Bacterial community in the microenvironment of the sponge.
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effect of copper treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Mbio, 2014Co-Authors: Renmao Tian, Yong Wang, Salim Bougouffa, Weipeng Zhang, Vladimir B Bajic, Pei-yuan QianAbstract:Marine sponges are the most primitive metazoan and host symbiotic microorganisms. They are crucial components of the marine ecological system and play an essential role in pelagic processes. Copper pollution is currently a widespread problem and poses a threat to marine organisms. Here, we examined the effects of copper treatment on the composition of the sponge- associated Bacterial community and the genetic features that facilitate the survival of enriched Bacteria under copper stress. The 16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothi- orhodospiraceae and photosynthetic CyanoBacteriaas dominant species. However, these Autotrophic Bacteria decreased sub- stantially after treatment with a high copper concentration, which enriched for a heterotrophic-bacterium-dominated commu- nity. Metagenomic comparison revealed a varied profile of functional genes and enriched functions, including Bacterial motility and chemotaxis, extracellular polysaccharide and capsule synthesis, virulence-associated genes, and genes involved in cell signal- ing and regulation, suggesting short-period mechanisms of the enriched Bacterial community for surviving copper stress in the microenvironment of the sponge. Microscopic observation and comparison revealed dynamic Bacterial aggregation within the matrix and lysis of sponge cells. The bacteriophage community was also enriched, and the complete genome of a dominant phage was determined, implying that a lytic phage cycle was stimulated by the high copper concentration. This study demonstrated a copper-induced shift in the composition of functional genes of the sponge-associated Bacterial community, revealing the selec- tive effect of copper treatment on the functions of the Bacterial community in the microenvironment of the sponge. IMPORTANCE This study determined the Bacterial community structure of the common sponge Haliclona cymaeformis and ex- amined the effect of copper treatment on the community structure and functional gene composition, revealing that copper treat- ment had a selective effect on the functions of the Bacterial community in the sponge. Thesefindings suggest that copper pollu- tion has an ecological impact on the sponge symbiont. The analysis showed that the untreated sponges hosted symbiotic Autotrophic Bacteria as dominant species, and the high-concentration copper treatment enriched for a heterotrophic Bacterial community with enrichment for genes important for Bacterial motility, supplementary cellular components, signaling and regu- lation, and virulence. Microscopic observation showed obvious Bacterial aggregation and a reduction of sponge cell numbers in treated sponges, which suggested the formation of aggregates to reduce the copper concentration. The enrichment for functions of directional Bacterial movement and supplementary cellular components and the formation of Bacterial aggregates and phage enrichment are novelfindings in sponge studies.
Salim Bougouffa - One of the best experts on this subject based on the ideXlab platform.
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effect of polybrominated diphenyl ether pbde treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Frontiers in Microbiology, 2015Co-Authors: Renmao Tian, Yong Wang, Pei-yuan Qian, Salim Bougouffa, Jill M Y Chiu, Rudolf S S WuAbstract:Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the Bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the Bacterial community shifted from an Autotrophic Bacteria-dominated community to a heterotrophic Bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 µg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic Bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic Bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and Bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated Bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the Bacterial community in the microenvironment of the sponge.
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effect of copper treatment on the composition and function of the Bacterial community in the sponge haliclona cymaeformis
Mbio, 2014Co-Authors: Renmao Tian, Yong Wang, Salim Bougouffa, Weipeng Zhang, Vladimir B Bajic, Pei-yuan QianAbstract:Marine sponges are the most primitive metazoan and host symbiotic microorganisms. They are crucial components of the marine ecological system and play an essential role in pelagic processes. Copper pollution is currently a widespread problem and poses a threat to marine organisms. Here, we examined the effects of copper treatment on the composition of the sponge- associated Bacterial community and the genetic features that facilitate the survival of enriched Bacteria under copper stress. The 16S rRNA gene sequencing results showed that the sponge Haliclona cymaeformis harbored symbiotic sulfur-oxidizing Ectothi- orhodospiraceae and photosynthetic CyanoBacteriaas dominant species. However, these Autotrophic Bacteria decreased sub- stantially after treatment with a high copper concentration, which enriched for a heterotrophic-bacterium-dominated commu- nity. Metagenomic comparison revealed a varied profile of functional genes and enriched functions, including Bacterial motility and chemotaxis, extracellular polysaccharide and capsule synthesis, virulence-associated genes, and genes involved in cell signal- ing and regulation, suggesting short-period mechanisms of the enriched Bacterial community for surviving copper stress in the microenvironment of the sponge. Microscopic observation and comparison revealed dynamic Bacterial aggregation within the matrix and lysis of sponge cells. The bacteriophage community was also enriched, and the complete genome of a dominant phage was determined, implying that a lytic phage cycle was stimulated by the high copper concentration. This study demonstrated a copper-induced shift in the composition of functional genes of the sponge-associated Bacterial community, revealing the selec- tive effect of copper treatment on the functions of the Bacterial community in the microenvironment of the sponge. IMPORTANCE This study determined the Bacterial community structure of the common sponge Haliclona cymaeformis and ex- amined the effect of copper treatment on the community structure and functional gene composition, revealing that copper treat- ment had a selective effect on the functions of the Bacterial community in the sponge. Thesefindings suggest that copper pollu- tion has an ecological impact on the sponge symbiont. The analysis showed that the untreated sponges hosted symbiotic Autotrophic Bacteria as dominant species, and the high-concentration copper treatment enriched for a heterotrophic Bacterial community with enrichment for genes important for Bacterial motility, supplementary cellular components, signaling and regu- lation, and virulence. Microscopic observation showed obvious Bacterial aggregation and a reduction of sponge cell numbers in treated sponges, which suggested the formation of aggregates to reduce the copper concentration. The enrichment for functions of directional Bacterial movement and supplementary cellular components and the formation of Bacterial aggregates and phage enrichment are novelfindings in sponge studies.
Duofu Chen - One of the best experts on this subject based on the ideXlab platform.
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using bathymodiolus tissue stable carbon nitrogen and sulfur isotopes to infer biogeochemical process at a cold seep in the south china sea
Deep Sea Research Part I: Oceanographic Research Papers, 2015Co-Authors: Dong Feng, Ming Cheng, Steffen Kiel, Qunhui Yang, Huaiyang Zhou, Yongbo Peng, Duofu ChenAbstract:Abstract More than 30 sites of active and inactive methane seepage have been discovered on the continental slope of the South China Sea over the past decade. Here we use stable isotopes of carbon, nitrogen and sulfur in the tissue of two bathymodiolin mussel species with different chemotrophic symbionts (methanotrophs in Bathymodiolus platifrons and sulfide-oxidizers in Bathymodiolus aduloides) to gain insights into the biogeochemical processes at an active site in 1120 m depth on the Formosa Ridge, called Site F. Because mussels with methanotrophic symbionts acquire the isotope signature of the used methane, the average δ13Ctissue values of B. platifrons (−70.3‰; n=36) indicates a biogenic methane source at Site F, consistent with the measured carbon isotope signature of methane (–61.1‰ to –58.7‰) sampled 1.5 m above the mussel beds. The only small offset between the δ13C signatures of the ascending methane and the authigenic carbonate at site F (as low as −55.3‰) suggests only minor mixing of the pore water with marine bicarbonate, which in turn may be used as an indicator for advective rather than diffusive seepage at this site. Bathymodiolus aduloides has much higher average δ13Ctissue values of −34.4‰ (n=9), indicating inorganic carbon (DIC) dissolved in epibenthic bottom water as its main carbon source. The DIC was apparently marine bicarbonate with a small contribution of 13C-depleted carbon from locally oxidized methane. The δ34S values of the two mussel species indicate that they used two different sulfur sources. Bathymodiolus platifrons (average δ34S=+6.4±2.6‰; n=36) used seawater sulfate mixed with isotopically light re-oxidized sulfide from the sulfate-dependent anaerobic oxidation of methane (AOM), while the sulfur source of B. aduloides (δ34S=−8.0±3.1‰; n=9) was AOM-derived sulfide used by its symbionts. δ15Ntissue values differed between the mussels, with B. platifrons having a wider range of on average slightly lower values (mean=+0.5±0.7‰, n=36) than B. aduloides (mean=+1.1±0.0‰). These values are significantly lower than δ15N values of South China Sea deep-sea sediments (+5‰ to +6‰), indicating that the organic nitrogen is of local origin, possibly resulting from the activity of Autotrophic Bacteria and due to assimilation of isotopically light nitrate or ammonium by the symbionts.