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Claudio L Afonso - One of the best experts on this subject based on the ideXlab platform.
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Identification of Avian Paramyxovirus Serotype-1 in Wild Birds in the USA
Journal of Wildlife Diseases, 2016Co-Authors: Kerri Pedersen, Kiril M. Dimitrov, Patti J Miller, Claudio L Afonso, David R. Marks, Scott R. Stopak, Dawn Williams-coplin, Thomas J. DelibertoAbstract:Abstract In the US, sampling for Avian Paramyxovirus serotype-1 (APMV-1) is generally conducted when morbidity or mortality events occur involving certain families of wild birds known to be affected by the virus, such as cormorants (Family Phalacrocoracidae), pigeons, doves (Family Columbidae), or pelicans (Family Pelecanidae). To quantify the prevalence of APMV-1 in apparently healthy wild birds and to determine its geographic distribution, we collected swab and serum samples from >3,500 wild birds, representing eight orders from 1 January 2013 to 30 September 2013. Antibody prevalence was highest in wild birds of Order Suliformes (44.9%), followed by Pelecaniformes (24.4%), Anseriformes (22.7%), and Columbiformes (11.7%), with a relatively high occurrence of virulent viruses in Columbiformes (100% of virulent viruses isolated). As expected, viral shedding was comparatively much lower, and positives were only identified in Orders Accipitriformes (1.4%), Columbiformes (1.0%), Anseriformes (0.8%), and Char...
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and
Avian Diseases, 2014Co-Authors: Kerri Pedersen, Patti J Miller, Claudio L Afonso, David R. Marks, Dustin M. Arsnoe, Sarah N. Bevins, Adam R. Randall, Thomas J. DelibertoAbstract:Since their introduction to the United States in the late 19th century, mute swans (Cygnus olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish Avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for Avian Paramyxovirus serotype 1 (APMV-1), Avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
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genetic diversity of Avian Paramyxovirus type 1 proposal for a unified nomenclature and classification system of newcastle disease virus genotypes
Infection Genetics and Evolution, 2012Co-Authors: Diego G Diel, Luciana H A Da Silva, Hualei Liu, Patti J Miller, Zhiliang Wang, Claudio L AfonsoAbstract:Abstract The Avian Paramyxovirus type 1 (APMV-1), or Newcastle disease virus (NDV), comprise a diverse group of viruses with a single-stranded, negative-sense RNA genome. Historically, two systems have been simultaneously used to classify NDV isolates into lineages or genotypes, generating confusion in the nomenclature and discrepancies in the assignment of genetic groups. In the present study we assessed the genetic diversity of the Avian Paramyxovirus type-1 (APMV-1) and propose a unified nomenclature and a classification system based on objective criteria to separate NDV into genotypes. Complete F gene sequences of class I ( n = 110) and class II ( n = 602) viruses were used for the phylogenetic reconstruction and to identify distinct taxonomic groups. The mean interpopulational evolutionary distance was estimated (10%) and set as the cutoff value to assign new genotypes. Results of our study revealed that class I viruses comprise a single genotype, while class II contains 15 genetic groups including 10 previously established (I–IX, and XI) and five new genotypes (X, XII, XIII, XIV and XV). Sub-genotypes were identified among class I and class II genotypes. Adoption of a unified nomenclature and of objective criteria to classify NDV isolates will facilitate studies on NDV epidemiology, evolution, disease control and diagnostics.
Kang-seuk Choi - One of the best experts on this subject based on the ideXlab platform.
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Genetic Diversity of Avian Paramyxovirus Type 6 Isolated from Wild Ducks in the Republic of Korea
Journal of wildlife diseases, 2018Co-Authors: Kang-seuk Choi, Hyun-jeong Lee, Ji-ye Kim, Hyuk-moo Kwon, Min-jun Jang, Haan-woo SungAbstract:Abstract: Eleven Avian Paramyxovirus type 6 (APMV-6) isolates from Eurasian Wigeon (n=5; Anas penelope), Mallards (n=2; Anas platyrhynchos), and unknown species of wild ducks (n=4) from Korea were analyzed based on the nucleotide (nt) and deduced amino acid sequences of the fusion (F) gene. Fecal samples were collected in 2010–14. Genotypes were assigned based on phylogenetic analyses. Our results revealed that APMV-6 could be classified into at least two distinct genotypes, G1 and G2. The open reading frame (ORF) of the G1 genotype was 1,668 nt in length, and the putative F0 cleavage site sequence was 113PAPEPRL119. The G2 genotype viruses included five isolates from Eurasian wigeons and four isolates from unknown waterfowl species, together with two reference APMV-6 strains from the Red-necked Stint (Calidris ruficollis) from Japan and an unknown duck from Italy. There was an N-truncated ORF (1,638 nt), due to an N-terminal truncation of 30 nt in the signal peptide region of the F gene, and the putativ...
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complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in south korea
Archives of Virology, 2018Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku Oem, Weonhwa JheongAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (< 63%) and a unique genomic composition. Phylogenetic analysis revealed that Cheonsu1510 was related to but distinct from APMV-1, -9, and -15. These results suggest that Cheonsu1510 represents a new APMV serotype, APMV-17.
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Complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in South Korea
Archives of virology, 2017Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku OemAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (
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Baculovirus expression of the Avian Paramyxovirus 2 HN gene for diagnostic applications
Journal of virological methods, 2013Co-Authors: Kang-seuk Choi, Soo-jeong Kye, Ji-ye Kim, Hee-jeong Seul, Hee-soo Lee, Hyuk-moo Kwon, Haan-woo SungAbstract:Avian Paramyxovirus 2 (APMV-2) infections are associated with respiratory diseases in poultry worldwide. The hemagglutination inhibition (HI) test is a useful tool for surveillance and monitoring of this virus. In this study, full-length hemagglutinin (HN) gene of APMV-2 was chemically synthesized based on its published sequence, cloned and expressed in Spodoptera frugiperda insect cells using recombinant baculoviruses. The biological, antigenic and immunogenic properties of the expressed protein were evaluated to assess its ability to produce diagnostic reagents for HI testing. Recombinant APMV-2 HN protein showed two distinct bands with molecular masses of 64 and 75kDa, which showed hemagglutination (HA) and neuraminidase activities, respectively. The recombinant HN (rHN) protein extracted from infected cells produced high HA titers (2(13) per 25μL). HA activity of the protein was inhibited by APMV-2 antiserum, although there were weak cross reactions with other APMV serotype antisera. The rHN protein induced high titers of APMV-2-specific antibodies in immunized chickens based on the HI test. These results indicated that recombinant APMV-2 HN protein is a useful alternative to the APMV-2 antigen in HI assays.
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Full-length genome sequence of Avian Paramyxovirus type 4 isolated from a mallard duck
Virus Genes, 2008Co-Authors: Woo-jin Jeon, Jun-hun Kwon, Kang-seuk ChoiAbstract:Avian Paramyxovirus (APMV) consists of nine serotypes, APMV-1 through -9, of which only APMV-1 and APMV-6 have been fully sequenced. Here, we present the complete 15,054 nt RNA genome of APMV-4 isolated from a mallard duck, which conformed to the “rule of six.” The APMV-4 genome had six transcriptional units in the order 3′-NP-P/V-M-F-HN-L-5′, which coded for the nucleocapsid (N), phospho- (P), matrix (M), fusion (F), hemagglutinin-neuraminidase (HN), and large (L) proteins. Similar to APMV-1 but unlike APMV-6, APMV-4 lacked a small hydrophobic protein. The leader and trailer sequences were 55 and 17 nt in length, respectively, and the 12 nt-terminal regions of both ends of the APMV-4 genome were complementary. Using phylogenetic analysis, APMV-4 was classified as a member of the genus Avulavirus , and was more closely related to APMV-1 than to APMV-2 or APMV-6. These results may help establish the taxonomic position of Paramyxoviridae , Avulavirus members.
Jae-ku Oem - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in south korea
Archives of Virology, 2018Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku Oem, Weonhwa JheongAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (< 63%) and a unique genomic composition. Phylogenetic analysis revealed that Cheonsu1510 was related to but distinct from APMV-1, -9, and -15. These results suggest that Cheonsu1510 represents a new APMV serotype, APMV-17.
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Complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in South Korea
Archives of virology, 2017Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku OemAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (
Patti J Miller - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of an Avian Paramyxovirus representative of putative new serotype 13
Genome Announcements, 2016Co-Authors: Iryna V Goraichuk, Denys Muzyka, Anton Gerilovych, O Solodiankin, Vitaliy Bolotin, Mary J Pantinjackwood, B. T. Stegniy, Patti J Miller, Poonam Sharma, Kiril M. DimitrovAbstract:ABSTRACT Here, we report the complete genome sequence of a virus of a putative new serotype of Avian Paramyxovirus (APMV). The virus was isolated from a white-fronted goose in Ukraine in 2011 and designated white-fronted goose/Ukraine/Askania-Nova/48-15-02/2011. The genomic characterization of the isolate suggests that it represents the novel Avian Paramyxovirus group APMV 13.
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Identification of Avian Paramyxovirus Serotype-1 in Wild Birds in the USA
Journal of Wildlife Diseases, 2016Co-Authors: Kerri Pedersen, Kiril M. Dimitrov, Patti J Miller, Claudio L Afonso, David R. Marks, Scott R. Stopak, Dawn Williams-coplin, Thomas J. DelibertoAbstract:Abstract In the US, sampling for Avian Paramyxovirus serotype-1 (APMV-1) is generally conducted when morbidity or mortality events occur involving certain families of wild birds known to be affected by the virus, such as cormorants (Family Phalacrocoracidae), pigeons, doves (Family Columbidae), or pelicans (Family Pelecanidae). To quantify the prevalence of APMV-1 in apparently healthy wild birds and to determine its geographic distribution, we collected swab and serum samples from >3,500 wild birds, representing eight orders from 1 January 2013 to 30 September 2013. Antibody prevalence was highest in wild birds of Order Suliformes (44.9%), followed by Pelecaniformes (24.4%), Anseriformes (22.7%), and Columbiformes (11.7%), with a relatively high occurrence of virulent viruses in Columbiformes (100% of virulent viruses isolated). As expected, viral shedding was comparatively much lower, and positives were only identified in Orders Accipitriformes (1.4%), Columbiformes (1.0%), Anseriformes (0.8%), and Char...
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Avian Paramyxovirus Serotype 1 (Newcastle Disease Virus), Avian Influenza Virus, and
Avian Diseases, 2014Co-Authors: Kerri Pedersen, Patti J Miller, Claudio L Afonso, David R. Marks, Dustin M. Arsnoe, Sarah N. Bevins, Adam R. Randall, Thomas J. DelibertoAbstract:Since their introduction to the United States in the late 19th century, mute swans (Cygnus olor) have become a nuisance species by causing damage to aquatic habitats, acting aggressively toward humans, competing with native waterfowl, and potentially transmitting or serving as a reservoir of infectious diseases to humans and poultry. In an effort to investigate their potential role as a disease reservoir and to establish Avian health baselines for pathogens that threaten agricultural species or human health, we collected samples from 858 mute swans and tested them for Avian Paramyxovirus serotype 1 (APMV-1), Avian influenza virus (AIV), and Salmonella spp. when possible. Our results indicate that exposure to APMV-1 and AIV is common (60%, n 5 771, and 45%, n 5 344, antibody prevalence, respectively) in mute swans, but detection of active viral shedding is less common (8.7%, n 5 414, and 0.8%, n 5 390, respectively). Salmonella was isolated from three mute swans (0.6%, n 5 459), and although the serovars identified have been implicated in previous human outbreaks, it does not appear that Salmonella is commonly carried by mute swans.
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genetic diversity of Avian Paramyxovirus type 1 proposal for a unified nomenclature and classification system of newcastle disease virus genotypes
Infection Genetics and Evolution, 2012Co-Authors: Diego G Diel, Luciana H A Da Silva, Hualei Liu, Patti J Miller, Zhiliang Wang, Claudio L AfonsoAbstract:Abstract The Avian Paramyxovirus type 1 (APMV-1), or Newcastle disease virus (NDV), comprise a diverse group of viruses with a single-stranded, negative-sense RNA genome. Historically, two systems have been simultaneously used to classify NDV isolates into lineages or genotypes, generating confusion in the nomenclature and discrepancies in the assignment of genetic groups. In the present study we assessed the genetic diversity of the Avian Paramyxovirus type-1 (APMV-1) and propose a unified nomenclature and a classification system based on objective criteria to separate NDV into genotypes. Complete F gene sequences of class I ( n = 110) and class II ( n = 602) viruses were used for the phylogenetic reconstruction and to identify distinct taxonomic groups. The mean interpopulational evolutionary distance was estimated (10%) and set as the cutoff value to assign new genotypes. Results of our study revealed that class I viruses comprise a single genotype, while class II contains 15 genetic groups including 10 previously established (I–IX, and XI) and five new genotypes (X, XII, XIII, XIV and XV). Sub-genotypes were identified among class I and class II genotypes. Adoption of a unified nomenclature and of objective criteria to classify NDV isolates will facilitate studies on NDV epidemiology, evolution, disease control and diagnostics.
Jipseol Jeong - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in south korea
Archives of Virology, 2018Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku Oem, Weonhwa JheongAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (< 63%) and a unique genomic composition. Phylogenetic analysis revealed that Cheonsu1510 was related to but distinct from APMV-1, -9, and -15. These results suggest that Cheonsu1510 represents a new APMV serotype, APMV-17.
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Complete genome sequence of a novel Avian Paramyxovirus isolated from wild birds in South Korea
Archives of virology, 2017Co-Authors: Jipseol Jeong, Youngsik Kim, Seung-jun Wang, Yongkwan Kim, Hyun-jeong Lee, Kang-seuk Choi, Wongi Min, Jae-ku OemAbstract:A novel Avian Paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3’-N-P-M-F-HN-L-5’), and showed low sequence identity to other APMVs (
