The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
Manabu Sakakibara - One of the best experts on this subject based on the ideXlab platform.
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protein kinase c mediates memory consolidation of taste Avoidance Conditioning in lymnaea stagnalis
Neurobiology of Learning and Memory, 2014Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Alan M Kuzirian, Daniel L Alkon, Manabu SakakibaraAbstract:Abstract In Lymnaea stagnalis , in order to obtain a 10 min short-term memory (STM) of taste Avoidance Conditioning (TAC) at least 10 paired presentations of a conditioned stimulus (CS), sucrose, and an unconditioned stimulus (US), tactile stimulation to the animal’s head, are required. Pre-exposure of snails to the protein kinase C (PKC) α and e activator bryostatin (Bryo) facilitated STM formation in that only 5 paired CS–US trials were required. Typically 20 paired presentations of the CS–US are required for formation of STM and LTM. However, 20 paired presentations do not result in STM or LTM if snails are pre-incubated with a PKC inhibitor, Ro-32-0432. We also found that LTM lasting longer than 48 h was acquired with Bryo incubation for 45 min even after termination of the Conditioning paradigm. These data suggest that activation of the α and e isozymes of PKC is crucially involved in the formation of LTM and provide further support for a mechanism that has been conserved across the evolution of species ranging from invertebrate molluscs to higher mammals.
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Spaced taste Avoidance Conditioning in Lymnaea.
Neurobiology of learning and memory, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract We succeeded in taste Avoidance Conditioning with sucrose as the conditional stimulus (CS) and an electrical stimulus (∼1000 V, 80 μA) as the unconditional stimulus (US). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM) persisting for at least one week. However, while STM was elicited with 5, 8, 10, and 20 paired presentations of the CS–US on a single day, LTM was not. We found, however, that if we inserted a 3 h interval between a first and a second set of CS–US pairings that both 8 and 20 paired CS–US presentations on a single day was now sufficient to cause LTM formation. Exposing snails to bryostatin before or during training enhanced LTM formation such that 8 paired presentations of the CS–US resulted in LTM.
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high voltage with little current as an unconditional stimulus for taste Avoidance Conditioning in lymnaea stagnalis
Neuroscience Letters, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract A new and better taste Avoidance Conditioning paradigm for Lymnaea has been developed that replaces the previously used tactile unconditional stimulus (US) with an brief electrical stimulus (1000 V, 80 μA), while continuing to use a sucrose application to the lips as the conditional stimulus (CS). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM). The LTM persisted for at least one week. While STM was elicited with 5, 8, or 10 paired presentations of the CS–US on a single day, LTM was not. The new US used here was more consistent than the previously used US, and this stimulus consistency may explain why 15 paired CS–US presentations now result in LTM formation.
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critical period of memory enhancement during taste Avoidance Conditioning in lymnaea stagnalis
PLOS ONE, 2013Co-Authors: Tomoyo Takahashi, Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:The present study investigated the optimal training procedure leading to long-lasting taste Avoidance behavior in Lymnaea. A training procedure comprising 5 repeated pairings of a conditional stimulus (CS, sucrose), with an unconditional stimulus (US, a tactile stimulation to the animal’s head), over a 4-day period resulted in an enhanced memory formation than 10 CS-US repeated pairings over a 2-day period or 20 CS-US repeated pairings on a single day. Backward Conditioning (US-CS) pairings did not result in Conditioning. Thus, this taste Avoidance Conditioning was CS-US pairing specific. Food Avoidance behavior was not observed following training, however, if snails were immediately subjected to a cold-block (4°C for 10 min). It was critical that the cold-block be applied within 10 min to block long-term memory (LTM) formation. Further, exposure to the cold-block 180 min after training also blocked both STM and LTM formation. The effects of the cold-block on subsequent learning and memory formation were also examined. We found no long lasting effects of the cold-block on subsequent memory formation. If protein kinase C was activated before the Conditioning paradigm, snails could still acquire STM despite exposure to the cold-block.
Satoshi Takigami - One of the best experts on this subject based on the ideXlab platform.
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protein kinase c mediates memory consolidation of taste Avoidance Conditioning in lymnaea stagnalis
Neurobiology of Learning and Memory, 2014Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Alan M Kuzirian, Daniel L Alkon, Manabu SakakibaraAbstract:Abstract In Lymnaea stagnalis , in order to obtain a 10 min short-term memory (STM) of taste Avoidance Conditioning (TAC) at least 10 paired presentations of a conditioned stimulus (CS), sucrose, and an unconditioned stimulus (US), tactile stimulation to the animal’s head, are required. Pre-exposure of snails to the protein kinase C (PKC) α and e activator bryostatin (Bryo) facilitated STM formation in that only 5 paired CS–US trials were required. Typically 20 paired presentations of the CS–US are required for formation of STM and LTM. However, 20 paired presentations do not result in STM or LTM if snails are pre-incubated with a PKC inhibitor, Ro-32-0432. We also found that LTM lasting longer than 48 h was acquired with Bryo incubation for 45 min even after termination of the Conditioning paradigm. These data suggest that activation of the α and e isozymes of PKC is crucially involved in the formation of LTM and provide further support for a mechanism that has been conserved across the evolution of species ranging from invertebrate molluscs to higher mammals.
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Spaced taste Avoidance Conditioning in Lymnaea.
Neurobiology of learning and memory, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract We succeeded in taste Avoidance Conditioning with sucrose as the conditional stimulus (CS) and an electrical stimulus (∼1000 V, 80 μA) as the unconditional stimulus (US). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM) persisting for at least one week. However, while STM was elicited with 5, 8, 10, and 20 paired presentations of the CS–US on a single day, LTM was not. We found, however, that if we inserted a 3 h interval between a first and a second set of CS–US pairings that both 8 and 20 paired CS–US presentations on a single day was now sufficient to cause LTM formation. Exposing snails to bryostatin before or during training enhanced LTM formation such that 8 paired presentations of the CS–US resulted in LTM.
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high voltage with little current as an unconditional stimulus for taste Avoidance Conditioning in lymnaea stagnalis
Neuroscience Letters, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract A new and better taste Avoidance Conditioning paradigm for Lymnaea has been developed that replaces the previously used tactile unconditional stimulus (US) with an brief electrical stimulus (1000 V, 80 μA), while continuing to use a sucrose application to the lips as the conditional stimulus (CS). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM). The LTM persisted for at least one week. While STM was elicited with 5, 8, or 10 paired presentations of the CS–US on a single day, LTM was not. The new US used here was more consistent than the previously used US, and this stimulus consistency may explain why 15 paired CS–US presentations now result in LTM formation.
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critical period of memory enhancement during taste Avoidance Conditioning in lymnaea stagnalis
PLOS ONE, 2013Co-Authors: Tomoyo Takahashi, Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:The present study investigated the optimal training procedure leading to long-lasting taste Avoidance behavior in Lymnaea. A training procedure comprising 5 repeated pairings of a conditional stimulus (CS, sucrose), with an unconditional stimulus (US, a tactile stimulation to the animal’s head), over a 4-day period resulted in an enhanced memory formation than 10 CS-US repeated pairings over a 2-day period or 20 CS-US repeated pairings on a single day. Backward Conditioning (US-CS) pairings did not result in Conditioning. Thus, this taste Avoidance Conditioning was CS-US pairing specific. Food Avoidance behavior was not observed following training, however, if snails were immediately subjected to a cold-block (4°C for 10 min). It was critical that the cold-block be applied within 10 min to block long-term memory (LTM) formation. Further, exposure to the cold-block 180 min after training also blocked both STM and LTM formation. The effects of the cold-block on subsequent learning and memory formation were also examined. We found no long lasting effects of the cold-block on subsequent memory formation. If protein kinase C was activated before the Conditioning paradigm, snails could still acquire STM despite exposure to the cold-block.
Hiroshi Sunada - One of the best experts on this subject based on the ideXlab platform.
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protein kinase c mediates memory consolidation of taste Avoidance Conditioning in lymnaea stagnalis
Neurobiology of Learning and Memory, 2014Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Alan M Kuzirian, Daniel L Alkon, Manabu SakakibaraAbstract:Abstract In Lymnaea stagnalis , in order to obtain a 10 min short-term memory (STM) of taste Avoidance Conditioning (TAC) at least 10 paired presentations of a conditioned stimulus (CS), sucrose, and an unconditioned stimulus (US), tactile stimulation to the animal’s head, are required. Pre-exposure of snails to the protein kinase C (PKC) α and e activator bryostatin (Bryo) facilitated STM formation in that only 5 paired CS–US trials were required. Typically 20 paired presentations of the CS–US are required for formation of STM and LTM. However, 20 paired presentations do not result in STM or LTM if snails are pre-incubated with a PKC inhibitor, Ro-32-0432. We also found that LTM lasting longer than 48 h was acquired with Bryo incubation for 45 min even after termination of the Conditioning paradigm. These data suggest that activation of the α and e isozymes of PKC is crucially involved in the formation of LTM and provide further support for a mechanism that has been conserved across the evolution of species ranging from invertebrate molluscs to higher mammals.
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Spaced taste Avoidance Conditioning in Lymnaea.
Neurobiology of learning and memory, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract We succeeded in taste Avoidance Conditioning with sucrose as the conditional stimulus (CS) and an electrical stimulus (∼1000 V, 80 μA) as the unconditional stimulus (US). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM) persisting for at least one week. However, while STM was elicited with 5, 8, 10, and 20 paired presentations of the CS–US on a single day, LTM was not. We found, however, that if we inserted a 3 h interval between a first and a second set of CS–US pairings that both 8 and 20 paired CS–US presentations on a single day was now sufficient to cause LTM formation. Exposing snails to bryostatin before or during training enhanced LTM formation such that 8 paired presentations of the CS–US resulted in LTM.
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high voltage with little current as an unconditional stimulus for taste Avoidance Conditioning in lymnaea stagnalis
Neuroscience Letters, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract A new and better taste Avoidance Conditioning paradigm for Lymnaea has been developed that replaces the previously used tactile unconditional stimulus (US) with an brief electrical stimulus (1000 V, 80 μA), while continuing to use a sucrose application to the lips as the conditional stimulus (CS). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM). The LTM persisted for at least one week. While STM was elicited with 5, 8, or 10 paired presentations of the CS–US on a single day, LTM was not. The new US used here was more consistent than the previously used US, and this stimulus consistency may explain why 15 paired CS–US presentations now result in LTM formation.
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critical period of memory enhancement during taste Avoidance Conditioning in lymnaea stagnalis
PLOS ONE, 2013Co-Authors: Tomoyo Takahashi, Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:The present study investigated the optimal training procedure leading to long-lasting taste Avoidance behavior in Lymnaea. A training procedure comprising 5 repeated pairings of a conditional stimulus (CS, sucrose), with an unconditional stimulus (US, a tactile stimulation to the animal’s head), over a 4-day period resulted in an enhanced memory formation than 10 CS-US repeated pairings over a 2-day period or 20 CS-US repeated pairings on a single day. Backward Conditioning (US-CS) pairings did not result in Conditioning. Thus, this taste Avoidance Conditioning was CS-US pairing specific. Food Avoidance behavior was not observed following training, however, if snails were immediately subjected to a cold-block (4°C for 10 min). It was critical that the cold-block be applied within 10 min to block long-term memory (LTM) formation. Further, exposure to the cold-block 180 min after training also blocked both STM and LTM formation. The effects of the cold-block on subsequent learning and memory formation were also examined. We found no long lasting effects of the cold-block on subsequent memory formation. If protein kinase C was activated before the Conditioning paradigm, snails could still acquire STM despite exposure to the cold-block.
Ken Lukowiak - One of the best experts on this subject based on the ideXlab platform.
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protein kinase c mediates memory consolidation of taste Avoidance Conditioning in lymnaea stagnalis
Neurobiology of Learning and Memory, 2014Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Alan M Kuzirian, Daniel L Alkon, Manabu SakakibaraAbstract:Abstract In Lymnaea stagnalis , in order to obtain a 10 min short-term memory (STM) of taste Avoidance Conditioning (TAC) at least 10 paired presentations of a conditioned stimulus (CS), sucrose, and an unconditioned stimulus (US), tactile stimulation to the animal’s head, are required. Pre-exposure of snails to the protein kinase C (PKC) α and e activator bryostatin (Bryo) facilitated STM formation in that only 5 paired CS–US trials were required. Typically 20 paired presentations of the CS–US are required for formation of STM and LTM. However, 20 paired presentations do not result in STM or LTM if snails are pre-incubated with a PKC inhibitor, Ro-32-0432. We also found that LTM lasting longer than 48 h was acquired with Bryo incubation for 45 min even after termination of the Conditioning paradigm. These data suggest that activation of the α and e isozymes of PKC is crucially involved in the formation of LTM and provide further support for a mechanism that has been conserved across the evolution of species ranging from invertebrate molluscs to higher mammals.
-
Spaced taste Avoidance Conditioning in Lymnaea.
Neurobiology of learning and memory, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract We succeeded in taste Avoidance Conditioning with sucrose as the conditional stimulus (CS) and an electrical stimulus (∼1000 V, 80 μA) as the unconditional stimulus (US). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM) persisting for at least one week. However, while STM was elicited with 5, 8, 10, and 20 paired presentations of the CS–US on a single day, LTM was not. We found, however, that if we inserted a 3 h interval between a first and a second set of CS–US pairings that both 8 and 20 paired CS–US presentations on a single day was now sufficient to cause LTM formation. Exposing snails to bryostatin before or during training enhanced LTM formation such that 8 paired presentations of the CS–US resulted in LTM.
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high voltage with little current as an unconditional stimulus for taste Avoidance Conditioning in lymnaea stagnalis
Neuroscience Letters, 2013Co-Authors: Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:Abstract A new and better taste Avoidance Conditioning paradigm for Lymnaea has been developed that replaces the previously used tactile unconditional stimulus (US) with an brief electrical stimulus (1000 V, 80 μA), while continuing to use a sucrose application to the lips as the conditional stimulus (CS). With 15 paired CS–US presentations on a single day, we were able to elicit both short-term memory (STM) and long-term memory (LTM). The LTM persisted for at least one week. While STM was elicited with 5, 8, or 10 paired presentations of the CS–US on a single day, LTM was not. The new US used here was more consistent than the previously used US, and this stimulus consistency may explain why 15 paired CS–US presentations now result in LTM formation.
-
critical period of memory enhancement during taste Avoidance Conditioning in lymnaea stagnalis
PLOS ONE, 2013Co-Authors: Tomoyo Takahashi, Satoshi Takigami, Hiroshi Sunada, Ken Lukowiak, Manabu SakakibaraAbstract:The present study investigated the optimal training procedure leading to long-lasting taste Avoidance behavior in Lymnaea. A training procedure comprising 5 repeated pairings of a conditional stimulus (CS, sucrose), with an unconditional stimulus (US, a tactile stimulation to the animal’s head), over a 4-day period resulted in an enhanced memory formation than 10 CS-US repeated pairings over a 2-day period or 20 CS-US repeated pairings on a single day. Backward Conditioning (US-CS) pairings did not result in Conditioning. Thus, this taste Avoidance Conditioning was CS-US pairing specific. Food Avoidance behavior was not observed following training, however, if snails were immediately subjected to a cold-block (4°C for 10 min). It was critical that the cold-block be applied within 10 min to block long-term memory (LTM) formation. Further, exposure to the cold-block 180 min after training also blocked both STM and LTM formation. The effects of the cold-block on subsequent learning and memory formation were also examined. We found no long lasting effects of the cold-block on subsequent memory formation. If protein kinase C was activated before the Conditioning paradigm, snails could still acquire STM despite exposure to the cold-block.
Ottavio Gandolfi - One of the best experts on this subject based on the ideXlab platform.
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Reduction of ECS-induced Retrograde Amnesia of Passive Avoidance Conditioning after 5,7-Dihydroxytryptamine Median Raphe Nucleus Lesion in the Rat
Neuropsychobiology, 2008Co-Authors: Nicola Montanaro, Rossella Dall'olio, Ottavio GandolfiAbstract:5,7-Dihydroxytryptamine (5,7-DHT), median raphe nucleus (MRN)-lesioned and sham-lesioned rats were submitted to one-trial passive Avoidance Conditioning followed by electroconvulsive shock (ECS) or sh
