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Axenic Culture

The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform

J W G Cairney – 1st expert on this subject based on the ideXlab platform

  • intra and interspecific variation in patterns of organic and inorganic nitrogen utilization by three australian pisolithus species
    Fungal Biology, 1999
    Co-Authors: Ian C. Anderson, Susan M. Chambers, J W G Cairney

    Abstract:

    The ability of three Australian Pisolithus species, discriminated on the basis of ITS sequence data, to utilize a range of inorganic and organic nitrogen sources was assessed in liquid Axenic Culture. Both intra-, and putative interspecific, variation in nitrogen source utilization was observed. Most isolates demonstrated a preference for NH 4 + over NO 3 − , although some showed no significant preference for either inorganic source. All isolates utilized a range of amino acids. Species I isolates demonstrated a preference for acidic and/or neutral amino acids over basic acids, while species II and III isolates generally utilized amino acids poorly relative to species I. Although most isolates utilized BSA poorly, two species I isolates that had been maintained in Axenic Culture for > 10 y grew well on this substrate, suggesting possible changes in nitrogen utilization with extended storage in Axenic Culture.

  • temperature regulation of extracellular proteases in ectomycorrhizal fungi hebeloma spp grown in Axenic Culture
    Fungal Biology, 1999
    Co-Authors: Mark Tibbett, F E Sanders, J W G Cairney, Jonathan R Leake

    Abstract:

    Strains of Hebeloma representative of different climatic zones were grown in Axenic Culture at either 2 °C and 22° or 6° and 22°. Culture filtrates were assayed for proteolytic activity using FITC labelled BSA as a substrate. Assays were run between 0–37°. Growth at low temperature induced greater proteolytic activity (g−1 D.W. mycelium). Many of the strains produced protease(s) which retained significant activity at temperatures as low as 0°, and a thermal optimum between 0–6° with a second optimum at higher temperature. The results are discussed in relation the nutrient acquisition potential of ectomycorrhizal fungi at low temperature and the contribution such cold active proteases might make to the soil enzyme pool.

Y. Yabu – 2nd expert on this subject based on the ideXlab platform

  • continuous growth of bloodstream forms of trypanosoma brucei brucei in an Axenic Culture system containing a low concentration of serum
    Southeast Asian Journal of Tropical Medicine and Public Health, 1998
    Co-Authors: Y. Yabu, Tatsuo Koide, Nobuo Ohta, Mitsuhiko Nose, Yukio Ogihara

    Abstract:

    : An effective Axenic Culture system for bloodstream forms of Trypanosoma brucei brucei GUT at 3.1 containing a low concentration of serum is described. Bloodstream forms routinely maintained in Iscove’s modification of Dulbecco’s medium supplemented with 100 microM hypoxanthine, 30 microM thymidine, 40 microM adenosine, 1 mM sodium pyruvate, 50 microM L-glutamine, 100 microM 2-mercaptoethanol and 20% FBS for more than one year were grown in the same medium supplemented with 5% FBS without reducing their growth rate. Then Culture adapted trypanosomes in the Culture medium containing 5% FBS were transferred into the modified medium supplemented with 0.5% FBS. For the constant growth of bloodstream forms in the medium containing 0.5% FBS, the Culture medium was further supplemented with 200 microM L-alanine, 100 microM glycine, 10 microM L-oruithine hydrochloride and 10 microM L-citrullin. The trypanosomes propagated in this Culture system for one year retained their infectivity for mice. This Culture system was also shown to be useful for cloning of T.b. brucei GUT at 3.1 which is important for separation of mutants.

  • an Axenic Culture system for the transformation of bloodstream forms to procyclic forms of trypanosoma brucei brucei in vitro
    Southeast Asian Journal of Tropical Medicine and Public Health, 1993
    Co-Authors: Y. Yabu

    Abstract:

    : An Axenic Culture system for continuous cultivation of bloodstream forms of Trypanosoma brucei GUT at 3.1 and subsequent transformation of bloodstream forms to procyclic forms is described. Bloodstream forms were continuously grown at 37 degrees C in Iscove’s modification of Dulbecco’s medium (M-DMEM, with bovine serum albumin, transferrin and soybean lecithin supplemented with 100 microM hypoxanthine, 30 microns thymidine, 40 microM adenosine, 1mM sodium pyruvate, 50 microM L-glutamine, 100 microM 2-mercaptoethanol and 20% (v/v) heat-inactivated fetal bovine serum. In this system, 2-mercaptoethanol (2-ME) was essential and in the absence of 2-ME, 100 microM L-cysteine and 10 microM bathocuproine sulfonate could not be substituted for 2-ME. This Culture system was useful for long-term Culture of bloodstream forms of this clone. Axenic cultivation of bloodstream forms at 27 degrees C resulted in transformation to procyclic forms within 5 days in the same medium supplemented with 5 mM L-proline, 8 micrograms/ml hemin and 4 micrograms/ml hematin, respectively and, instead of FBS, 20% (v/v) hemoglobin-poor fraction of fetal bovine serum.

  • An effective Axenic Culture system for Trypanosoma brucei rhodesiense blood stream forms in vitro.
    Southeast Asian Journal of Tropical Medicine and Public Health, 1990
    Co-Authors: Y. Yabu, T. Takayanagi, S. Sato

    Abstract:

    : An effective Axenic Culture system for Trypanosoma brucei rhodesiense (ILRAD 1501) bloodstream forms is demonstrated. Bloodstream forms were continuously grown in 25 mM HEPES-buffered D-MEM supplemented with 10 microM bathocuproine sulfonate (BCS), 100 microM cysteine, and 20% heat-inactivated fetal bovine serum at 37 degrees C in vitro. At the initiation of the Culture, T. b. rhodesiense bloodstream forms required the presence of 0.2 IU/ml insulin and 1 mM pyruvate, while bloodstream forms were grown in the Culture medium without these supplements 4 days after initiation of the Culture. Under this Culture condition, T. b. rhodesiense bloodstream forms increased in number to 7 to 8 x 10(6) trypanosomes/ml, by day 4 after initiation of the Culture. The trypanosomes Cultured in this Axenic system for 150 days were typically long and slender and retained their virulence for mice. This Axenic Culture system is extremely useful for in vitro cloning of T. b. rhodesiense bloodstream forms in vitro.

Jonathan R Leake – 3rd expert on this subject based on the ideXlab platform

  • temperature regulation of extracellular proteases in ectomycorrhizal fungi hebeloma spp grown in Axenic Culture
    Fungal Biology, 1999
    Co-Authors: Mark Tibbett, F E Sanders, J W G Cairney, Jonathan R Leake

    Abstract:

    Strains of Hebeloma representative of different climatic zones were grown in Axenic Culture at either 2 °C and 22° or 6° and 22°. Culture filtrates were assayed for proteolytic activity using FITC labelled BSA as a substrate. Assays were run between 0–37°. Growth at low temperature induced greater proteolytic activity (g−1 D.W. mycelium). Many of the strains produced protease(s) which retained significant activity at temperatures as low as 0°, and a thermal optimum between 0–6° with a second optimum at higher temperature. The results are discussed in relation the nutrient acquisition potential of ectomycorrhizal fungi at low temperature and the contribution such cold active proteases might make to the soil enzyme pool.