The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform
Jindřich Lokaj - One of the best experts on this subject based on the ideXlab platform.
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t and B Lymphocyte suBpopulations and activation differentiation markers in patients with selective iga deficiency
Clinical and Experimental Immunology, 2006Co-Authors: Jiři Litzman, Marcela Vlkova, Z Pikulova, Dagmar Stikarovska, Jindřich LokajAbstract:Selective deficiency of immunogloBulin A (IgAD) and common variaBle immunodeficiency (CVID) are genetically closely related diseases, Both of unknown pathogenesis. A plethora of aBnormalities in Lymphocyte suBpopulations and expression of activation markers were repeatedly documented in CVID patients, while almost no data are availaBle aBout Lymphocyte suBpopulations in IgAD patients. We determined Basic Lymphocyte suBpopulations and those suBpopulations that were reported to Be aBnormal in CVID patients (CD25, human leucocyte antigen (HLA)-DR CD45RA, CD45RO, CD27, CD28 and CD29 on Both CD4 + and CD8 + cells, CD57 and CD38 on CD8 + cells, CD21, CD27, IgM, IgD on B Lymphocytes) in 85 patients with IgAD, 47 patients with CVID and in 65 healthy controls. Statistical analysis was performed By the Mann-Whitney U-test; significant P-values were determined By means of Bonferoni's correction. Our results showed an increase in the relative numBer of CD8 + cells and a decrease in the aBsolute numBer of CD4 + cells compared to healthy people, But similar aBnormalities in CVID patients were much more expressed. IgAD patients had significantly decreased expression of HLA-DR and increased expression of CD25 on CD4 + Lymphocytes, also CD29 expression was decreased on CD8 + cells, while other activation/differentiation markers on T cells (including the expression of CD45RA and CD45RO antigens) were not changed. There were no statistically significant aBnormalities in B Lymphocyte developmental stages in IgAD patients compared to healthy controls. Our oBservation showed that the majority of T and B Lymphocyte suBpopulation aBnormalities descriBed previously in CVID are not present in IgAD patients.
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T and B Lymphocyte suBpopulations and activation/differentiation markers in patients with selective IgA deficiency
Clinical and experimental immunology, 2006Co-Authors: Jiři Litzman, Marcela Vlkova, Z Pikulova, Dagmar Stikarovska, Jindřich LokajAbstract:Selective deficiency of immunogloBulin A (IgAD) and common variaBle immunodeficiency (CVID) are genetically closely related diseases, Both of unknown pathogenesis. A plethora of aBnormalities in Lymphocyte suBpopulations and expression of activation markers were repeatedly documented in CVID patients, while almost no data are availaBle aBout Lymphocyte suBpopulations in IgAD patients. We determined Basic Lymphocyte suBpopulations and those suBpopulations that were reported to Be aBnormal in CVID patients (CD25, human leucocyte antigen (HLA)-DR CD45RA, CD45RO, CD27, CD28 and CD29 on Both CD4 + and CD8 + cells, CD57 and CD38 on CD8 + cells, CD21, CD27, IgM, IgD on B Lymphocytes) in 85 patients with IgAD, 47 patients with CVID and in 65 healthy controls. Statistical analysis was performed By the Mann-Whitney U-test; significant P-values were determined By means of Bonferoni's correction. Our results showed an increase in the relative numBer of CD8 + cells and a decrease in the aBsolute numBer of CD4 + cells compared to healthy people, But similar aBnormalities in CVID patients were much more expressed. IgAD patients had significantly decreased expression of HLA-DR and increased expression of CD25 on CD4 + Lymphocytes, also CD29 expression was decreased on CD8 + cells, while other activation/differentiation markers on T cells (including the expression of CD45RA and CD45RO antigens) were not changed. There were no statistically significant aBnormalities in B Lymphocyte developmental stages in IgAD patients compared to healthy controls. Our oBservation showed that the majority of T and B Lymphocyte suBpopulation aBnormalities descriBed previously in CVID are not present in IgAD patients.
Naoyuki Katayama - One of the best experts on this subject based on the ideXlab platform.
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Ontogeny of human B1 cells
International Journal of Hematology, 2020Co-Authors: Yuki Kageyama, Naoyuki KatayamaAbstract:B1 cells, which are distinct from conventional B cells, are a rare B Lymphocyte suBpopulation that plays a pivotal role in innate immunity. Extensive previous studies have revealed the functions and ontogeny of murine B1 cells, But the properties of human B1 cells have just Begun to Be uncovered over the past decade. The phenotype of human B1 cells has recently Been proposed, facilitating further studies. Here, we review the latest knowledge on human B1 cells, especially their ontogeny. A previous study using xenotransplantation models showed that human hematopoietic stem cells (HSCs) derived from cord Blood or adult Bone marrow can produce B1 cells in vivo. A recent study By our group reported that human B1 cells in peripheral Blood are derived from adult HSCs and persist for approximately 3 years in situ. These findings suggest that adult human HSCs have the aBility to produce B1 cells and contriBute to maintenance of the adult B1 cell pool in peripheral Blood. Further understanding of human B1 cell functions and ontogeny may elucidate the pathogenesis of B cell malignancies and autoimmune diseases.
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a population of cd20 cd27 cd43 cd38lo int B1 cells in pnh are missing gpi anchored proteins and harBor piga mutations
Blood, 2019Co-Authors: Yuki Kageyama, Hiroshi Miwa, Isao Tawara, Kohshi Ohishi, Masahiro Masuya, Naoyuki KatayamaAbstract:TO THE EDITOR: B1 cells were first descriBed in 1983 as a rare B-Lymphocyte suBpopulation that spontaneously secreted immunogloBulin M (IgM) and appeared to Be distinguished from B2 cells.[1][1] The functional properties, phenotype, and ontogeny of B1 cells differ from those of B2 cells.[2][2] In
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A population of CD20+CD27+CD43+CD38lo/int B1 cells in PNH are missing GPI-anchored proteins and harBor PIGA mutations.
Blood, 2019Co-Authors: Yuki Kageyama, Hiroshi Miwa, Isao Tawara, Kohshi Ohishi, Masahiro Masuya, Naoyuki KatayamaAbstract:TO THE EDITOR: B1 cells were first descriBed in 1983 as a rare B-Lymphocyte suBpopulation that spontaneously secreted immunogloBulin M (IgM) and appeared to Be distinguished from B2 cells.[1][1] The functional properties, phenotype, and ontogeny of B1 cells differ from those of B2 cells.[2][2] In
Jiři Litzman - One of the best experts on this subject based on the ideXlab platform.
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t and B Lymphocyte suBpopulations and activation differentiation markers in patients with selective iga deficiency
Clinical and Experimental Immunology, 2006Co-Authors: Jiři Litzman, Marcela Vlkova, Z Pikulova, Dagmar Stikarovska, Jindřich LokajAbstract:Selective deficiency of immunogloBulin A (IgAD) and common variaBle immunodeficiency (CVID) are genetically closely related diseases, Both of unknown pathogenesis. A plethora of aBnormalities in Lymphocyte suBpopulations and expression of activation markers were repeatedly documented in CVID patients, while almost no data are availaBle aBout Lymphocyte suBpopulations in IgAD patients. We determined Basic Lymphocyte suBpopulations and those suBpopulations that were reported to Be aBnormal in CVID patients (CD25, human leucocyte antigen (HLA)-DR CD45RA, CD45RO, CD27, CD28 and CD29 on Both CD4 + and CD8 + cells, CD57 and CD38 on CD8 + cells, CD21, CD27, IgM, IgD on B Lymphocytes) in 85 patients with IgAD, 47 patients with CVID and in 65 healthy controls. Statistical analysis was performed By the Mann-Whitney U-test; significant P-values were determined By means of Bonferoni's correction. Our results showed an increase in the relative numBer of CD8 + cells and a decrease in the aBsolute numBer of CD4 + cells compared to healthy people, But similar aBnormalities in CVID patients were much more expressed. IgAD patients had significantly decreased expression of HLA-DR and increased expression of CD25 on CD4 + Lymphocytes, also CD29 expression was decreased on CD8 + cells, while other activation/differentiation markers on T cells (including the expression of CD45RA and CD45RO antigens) were not changed. There were no statistically significant aBnormalities in B Lymphocyte developmental stages in IgAD patients compared to healthy controls. Our oBservation showed that the majority of T and B Lymphocyte suBpopulation aBnormalities descriBed previously in CVID are not present in IgAD patients.
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T and B Lymphocyte suBpopulations and activation/differentiation markers in patients with selective IgA deficiency
Clinical and experimental immunology, 2006Co-Authors: Jiři Litzman, Marcela Vlkova, Z Pikulova, Dagmar Stikarovska, Jindřich LokajAbstract:Selective deficiency of immunogloBulin A (IgAD) and common variaBle immunodeficiency (CVID) are genetically closely related diseases, Both of unknown pathogenesis. A plethora of aBnormalities in Lymphocyte suBpopulations and expression of activation markers were repeatedly documented in CVID patients, while almost no data are availaBle aBout Lymphocyte suBpopulations in IgAD patients. We determined Basic Lymphocyte suBpopulations and those suBpopulations that were reported to Be aBnormal in CVID patients (CD25, human leucocyte antigen (HLA)-DR CD45RA, CD45RO, CD27, CD28 and CD29 on Both CD4 + and CD8 + cells, CD57 and CD38 on CD8 + cells, CD21, CD27, IgM, IgD on B Lymphocytes) in 85 patients with IgAD, 47 patients with CVID and in 65 healthy controls. Statistical analysis was performed By the Mann-Whitney U-test; significant P-values were determined By means of Bonferoni's correction. Our results showed an increase in the relative numBer of CD8 + cells and a decrease in the aBsolute numBer of CD4 + cells compared to healthy people, But similar aBnormalities in CVID patients were much more expressed. IgAD patients had significantly decreased expression of HLA-DR and increased expression of CD25 on CD4 + Lymphocytes, also CD29 expression was decreased on CD8 + cells, while other activation/differentiation markers on T cells (including the expression of CD45RA and CD45RO antigens) were not changed. There were no statistically significant aBnormalities in B Lymphocyte developmental stages in IgAD patients compared to healthy controls. Our oBservation showed that the majority of T and B Lymphocyte suBpopulation aBnormalities descriBed previously in CVID are not present in IgAD patients.
Yuki Kageyama - One of the best experts on this subject based on the ideXlab platform.
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Ontogeny of human B1 cells
International Journal of Hematology, 2020Co-Authors: Yuki Kageyama, Naoyuki KatayamaAbstract:B1 cells, which are distinct from conventional B cells, are a rare B Lymphocyte suBpopulation that plays a pivotal role in innate immunity. Extensive previous studies have revealed the functions and ontogeny of murine B1 cells, But the properties of human B1 cells have just Begun to Be uncovered over the past decade. The phenotype of human B1 cells has recently Been proposed, facilitating further studies. Here, we review the latest knowledge on human B1 cells, especially their ontogeny. A previous study using xenotransplantation models showed that human hematopoietic stem cells (HSCs) derived from cord Blood or adult Bone marrow can produce B1 cells in vivo. A recent study By our group reported that human B1 cells in peripheral Blood are derived from adult HSCs and persist for approximately 3 years in situ. These findings suggest that adult human HSCs have the aBility to produce B1 cells and contriBute to maintenance of the adult B1 cell pool in peripheral Blood. Further understanding of human B1 cell functions and ontogeny may elucidate the pathogenesis of B cell malignancies and autoimmune diseases.
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a population of cd20 cd27 cd43 cd38lo int B1 cells in pnh are missing gpi anchored proteins and harBor piga mutations
Blood, 2019Co-Authors: Yuki Kageyama, Hiroshi Miwa, Isao Tawara, Kohshi Ohishi, Masahiro Masuya, Naoyuki KatayamaAbstract:TO THE EDITOR: B1 cells were first descriBed in 1983 as a rare B-Lymphocyte suBpopulation that spontaneously secreted immunogloBulin M (IgM) and appeared to Be distinguished from B2 cells.[1][1] The functional properties, phenotype, and ontogeny of B1 cells differ from those of B2 cells.[2][2] In
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A population of CD20+CD27+CD43+CD38lo/int B1 cells in PNH are missing GPI-anchored proteins and harBor PIGA mutations.
Blood, 2019Co-Authors: Yuki Kageyama, Hiroshi Miwa, Isao Tawara, Kohshi Ohishi, Masahiro Masuya, Naoyuki KatayamaAbstract:TO THE EDITOR: B1 cells were first descriBed in 1983 as a rare B-Lymphocyte suBpopulation that spontaneously secreted immunogloBulin M (IgM) and appeared to Be distinguished from B2 cells.[1][1] The functional properties, phenotype, and ontogeny of B1 cells differ from those of B2 cells.[2][2] In
Haruki Wakasa - One of the best experts on this subject based on the ideXlab platform.
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Phenotypic characterization of human B-Lymphocyte suBpopulations, particularly human CD5+ B-Lymphocyte suBpopulation within the mantle zones of secondary follicles.
Leukemia, 1994Co-Authors: Masafumi Abe, Kunihiko Tominaga, Haruki WakasaAbstract:We have investigated the precise distriBution of human B-Lymphocyte suBpopulations (CD5+ B Lymphocyte, Leu-8+ Lymphocyte, immunogloBulin D (IgD)+ Lymphocyte, alkaline phosphatase (ALPase)+ B Lymphocyte and Bcl-2 protein+ B Lymphocyte) within the mantle zones (MZs) and phenotypic characterization of human CD5+ B Lymphocytes using immunohistochemical techniques and flow cytometric analysis. IgD+ Lymphocytes and ALPase B Lymphocytes were confined to the inner layer and outer layer of the MZs of secondary follicles, respectively. CD5+ B Lymphocytes and Leu-8+ B Lymphocytes were mostly located in the inner layer of the MZs. Bcl-2 protein+ B Lymphocytes were seen throughout the MZs. The precise distriBution pattern of human B-Lymphocyte suBpopulations may help further understanding of the histogenesis and features of B-cell lymphomas, particularly mantle cell-derived lymphomas as well as the B-cell differentiation pathway. A minor population of CD5+ B Lymphocytes expressed IgD. Almost all the CD5+ Lymphocytes did not express ALPase. The data support the fact that CD5+ B Lymphocytes are located more in the inner layer than in the outer layer of the MZs. Leu-8 and Bcl-2 protein were detected in a large population of CD5+ B Lymphocytes. In addition, Ki-67 antigen was not expressed on the CD5+ B Lymphocytes. The data suggest that human CD5+ B Lymphocytes may Be long-living and resting (G0 and G1a stage) cells possessing the capaBility of continuously recirculating Between Blood and lymph nodes to participate in some immune responses. Moreover, Leu-8 and CD44 were detected in the majority of CD5+ B Lymphocytes But intercellular adhesion molecule-1 (ICAM-1) and very late antigen-4 (VLA-4) were detected in the minority. The data may account for a high percentage of Leu-8 and CD44 expression and a low percentage of ICAM-1 and VLA-4 expression on B-chronic lymphocytic leukemia (B-CLL), which is considered to Be a neoplastic counterpart of normal CD5+ B Lymphocyte.