The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform
Ben-ami Sela - One of the best experts on this subject based on the ideXlab platform.
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Splenocytes incorporated with exogenous gangliosides induce a mixed lymphocyte reaction in autologous lymphocytes
Cellular Immunology, 2004Co-Authors: Ben-ami SelaAbstract:Abstract SWR splenocytes incorporated with mixed gangliosides triggered an efficient mixed lymphocyte reaction in autologous thymus lymphocytes. The potency of the ganglioside-incorporated splenocytes as stimulators in the autologous system was eight to ninefold greater than background effect of untreated splenocytes. The induced mixed lymphocyte reaction was not due to free gangliosides in culture medium and occurred only if the gangliosides were incorporated into the triggering splenocytes, but not into the responding thymocytes. The mixed lymphocyte reaction generated by gangliosides incorporation in the autologous system is similar to that reaction in the allogeneic system with regard to the time course of [ 3 H]thymidine incorporation.
Elke Henrion - One of the best experts on this subject based on the ideXlab platform.
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Testing fish vaccines with an in vitro lymphocyte blastogenesis assay
ALTEX-Alternatives to Animal Experimentation, 1998Co-Authors: Ralf Peter Pund, Katrin Nold, Elke HenrionAbstract:: Lethal-challenge experiments are provided by German law till now for testing fish vaccines. Finding an in vitro-assay which could replace this experiments was subject of our work. Two questions must have been answered to develop the lymphocyte blastogenesis assay: Do lymphocytes of immunised fish react the same way as lymphocytes of naive fish, being incubated with antigen and is there a coherence between the lymphocyte's in-vitro-response and the immune status of challenged fish. Methods to take blood samples from anaesthetised fish and to obtain lymphocytes by centrifugation on gradients were worked out and the lymphocyte's demands on culture requirements were evaluated thoroughly because the ability to proliferate depends on many exogenous parameters. Proliferation was represented and measured by a tetrazolium based colorimetric assay (MTT).
Ralf Peter Pund - One of the best experts on this subject based on the ideXlab platform.
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Testing fish vaccines with an in vitro lymphocyte blastogenesis assay
ALTEX-Alternatives to Animal Experimentation, 1998Co-Authors: Ralf Peter Pund, Katrin Nold, Elke HenrionAbstract:: Lethal-challenge experiments are provided by German law till now for testing fish vaccines. Finding an in vitro-assay which could replace this experiments was subject of our work. Two questions must have been answered to develop the lymphocyte blastogenesis assay: Do lymphocytes of immunised fish react the same way as lymphocytes of naive fish, being incubated with antigen and is there a coherence between the lymphocyte's in-vitro-response and the immune status of challenged fish. Methods to take blood samples from anaesthetised fish and to obtain lymphocytes by centrifugation on gradients were worked out and the lymphocyte's demands on culture requirements were evaluated thoroughly because the ability to proliferate depends on many exogenous parameters. Proliferation was represented and measured by a tetrazolium based colorimetric assay (MTT).
Reinhard Pabst - One of the best experts on this subject based on the ideXlab platform.
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Intraepithelial lymphocytes in the lung: a neglected lymphocyte population.
American journal of respiratory cell and molecular biology, 2000Co-Authors: David J. Erle, Reinhard PabstAbstract:In the work presented in this issue, Goto and colleagues (1) have provided strong new evidence that a distinct population of lymphocytes resides within the bronchial epithelium. By following the fates of human bronchial xenografts after transplantation into SCID mice, these investigators were able to show long-term persistence (over 5 mo) of human intraepithelial lymphocytes (IEL) in grafts, even as the number of lymphocytes in the adjacent lamina propria declined precipitously. Although little is known about the function of bronchial IEL, their location suggests important roles in the control of immune and inflammatory reactions in the lung. Goto and coworkers’ work should prompt further investigation into the roles of bronchial IEL in health and disease.
A C G Heath - One of the best experts on this subject based on the ideXlab platform.
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Proliferative responses of lymphocytes in Bovicola ovis infested lambs
International journal for parasitology, 1995Co-Authors: J. Bany, A. Pfeffer, M.d. Phegan, A C G HeathAbstract:Abstract Proliferative responses of peripheral blood and prescapular lymph node lymphocytes from 8 B. ovis -infested and 8 valve lambs to concanavalin A (Con A) and soluble antigen of B. ovis were examined in vitro . The numbers of lice and the extent of cockle were also assessed on each lamb. Prescapular lymph node lymphocytes from infested lambs showed significantly higher responses to B. ovis antigen than those from naive lambs. Only a marginal difference was observed between infested and naive lambs in the response of lymphocytes from peripheral blood. Proliferative responses to Con A by prescapular lymph node or blood cells were not significantly different between groups. The results indicated a relationship between the intemity of louse infestation and the proliferative response of prescapular lymph node lymphocytec to B. ovis antigen.
