Bacterial Adhesion

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 37266 Experts worldwide ranked by ideXlab platform

Q Zhao - One of the best experts on this subject based on the ideXlab platform.

  • influence of surface energy components of ni p tio2 ptfe nanocomposite coatings on Bacterial Adhesion
    Langmuir, 2011
    Co-Authors: Chen Liu, Q Zhao
    Abstract:

    The influence of total surface energy on Bacterial Adhesion has been investigated intensively with the frequent conclusion that Bacterial Adhesion is less on low-energy surfaces. However, there are also a number of contrary findings that high-energy surfaces have a smaller biofouling tendency. Recently, it was found that the CQ ratio, which is defined as the ratio of Lifshitz–van der Waals (LW) apolar to electron donor surface-energy components of substrates, has a strong correlation to Bacterial Adhesion. However, the electron donor surface-energy components of substrates varied over only a very limited range. In this article, a series of Ni–P–TiO2–PTFE nanocomposite coatings with wide range of surface-energy components were prepared using an electroless plating technique. The Bacterial Adhesion and removal on the coatings were evaluated with different bacteria under both static and flow conditions. The experimental results demonstrated that there was a strong correlation between Bacterial attachment (or...

  • effect of corrosion rate and surface energy of silver coatings on Bacterial Adhesion
    Colloids and Surfaces B: Biointerfaces, 2010
    Co-Authors: Wei Shao, Q Zhao
    Abstract:

    Abstract Many studies suggest a strong antimicrobial activity of silver coatings. The biocidal activity of silver is related to the biologically active silver ion released from silver coatings. However, no studies have been reported on the effect of surface energy of silver coatings on antiBacterial performance. In this paper, three silver coatings with various corrosion rates and surface energies were prepared on stainless steel plates using AgNO3 based electroless plating solutions. The corrosion rate and surface energy of the silver coatings were characterized with CorrTest Electrochemistry Workstation and Dataphysics OCA-20 contact angle analyzer, respectively. The antiBacterial performance of the silver coatings was evaluated with Pseudomonas aeruginosa PA01, which frequently causes medical device-associated infections. The experimental results showed that surface energy had significant influence on initial Bacterial Adhesion at low corrosion rate. The extended DLVO theory was used to explain the Bacterial Adhesion behavior.

  • influence of reducers on nanostructure and surface energy of silver coatings and Bacterial Adhesion
    Surface & Coatings Technology, 2010
    Co-Authors: Wei Shao, Q Zhao
    Abstract:

    Abstract The high incidence of infections caused by the use of implanted biomedical devices has a severe impact on human health and health care costs. Many studies suggest a strong antimicrobial activity of silver-coated medical devices. However no studies have been reported on the effect of surface energy of silver coatings on Bacterial Adhesion. In this paper, 4 types of silver coatings with various surface energies were prepared on stainless steel plates using AgNO 3 based electroless plating solutions with four different reducing agents, formaldehyde, hydrazine, glucose and potassium sodium tartrate tetrahydrate, respectively. The morphology and surface energy of the silver coatings were characterized with an Atomic Force Microscope and a Dataphysics OCA-20 contact angle analyzer, respectively. The anti-Bacterial performance of the silver coatings was evaluated with Pseudomonas aeruginosa PA01, which frequently causes medical device-associated infections. The experimental results showed that Bacterial Adhesion decreased with the total surface energy of the coatings decreasing, but decreased with the electron donor component increasing. All the silver coatings performed much better than stainless steel in reducing Bacterial attachment. The extended DLVO theory was used to explain Bacterial Adhesion behavior.

  • reduction of Bacterial Adhesion on ion implanted stainless steel surfaces
    Medical Engineering & Physics, 2008
    Co-Authors: Q Zhao, S. Wang, Y. Liu, C. Wang, Nianhua Peng, C Jeynes
    Abstract:

    Abstract The high incidence of infections caused by the use of biomedical devices has a severe impact on human health. An approach to reduce the complications is to modify the surface properties of biomedical devices. In this paper, stainless steel disks were implanted with N + , O + and SiF 3 + , respectively, by an ion implantation technique. The surface properties of the ion-implanted surfaces were characterized, including their surface chemical composition, roughness, topography, wettability and surface energy. Bacterial Adhesion of Staphylococcus epidermidis and Staphylococcus aureus , which frequently cause medical device-associated infections was evaluated. The experimental results showed that these implanted stainless steels, particularly SiF 3 + implanted stainless steel performed much better than untreated stainless steel control on reducing Bacterial attachment.

  • Bacterial Adhesion on the metal polymer composite coatings
    International Journal of Adhesion and Adhesives, 2007
    Co-Authors: Q Zhao, C. Wang, Su Wang
    Abstract:

    Abstract Bacterial Adhesion on the surfaces of medical devices, food processing equipment, heat exchangers and ship hulls has been recognized as a widespread problem. Bacterial Adhesion mechanism is complex and many factors affect cell Adhesion. In this paper, the effect of surface free energy of the coatings on Bacterial Adhesion was investigated. The metal-polymer composite coatings with various surface free energies were developed by an electroless plating technique. Bacterial Adhesion behaviour on these coatings was investigated. Contact angles were obtained using a sessile drop method with a Dataphysics OCA-20 contact angle analyser. According to the contact angle values, the surface energies of the samples and their dispersive and polar components were calculated using van Oss acid–base approach. The experimental results showed that the surface free energy of the coatings had a significant influence on Bacterial Adhesion. The Bacterial Adhesion behaviour on the surfaces was explained using the extended DLVO theory.

Christopher A Siedlecki - One of the best experts on this subject based on the ideXlab platform.

  • Blood Coagulation Response and Bacterial Adhesion to Biomimetic Polyurethane Biomaterials Prepared with Surface Texturing and Nitric Oxide Release
    Acta Biomaterialia, 2018
    Co-Authors: Li-chong Xu, Mark E. Meyerhoff, Christopher A Siedlecki
    Abstract:

    Abstract A dual functional polyurethane (PU) film that mimics aspects of blood vessel inner surfaces by combining surface texturing and nitric oxide (NO) release was fabricated through a soft lithography two-stage replication process. The fabrication of submicron textures on the polymer surface was followed by solvent impregnation with the NO donor, S-nitroso-N-acetylpenicillamine (SNAP). An in vitro plasma coagulation assay showed that the biomimetic surface significantly increased the plasma coagulation time and also exhibited reduced platelet Adhesion and activation, thereby reducing the risk of blood coagulation and thrombosis. A contact activation assay for coagulation factor XII (FXII) demonstrated that both NO release and surface texturing also reduced FXII contact activation, which contributes to the inhibition of plasma coagulation. The biomimetic surface was also evaluated for Bacterial Adhesion in plasma and results demonstrate that this combined strategy enables a synergistic effect to reduce Bacterial Adhesion of Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa microorganisms. The results strongly suggest that the biomimetic modification with surface texturing and NO release provides an effective approach to improve the biocompatibility of polymeric materials in combating thrombosis and microbial infection. Statement of significance (1) Developed a dual functional polyurethane (PU) film that mimics blood vessel inner surface by combining surface texturing and nitric oxide (NO) release for combatting biomaterial associated thrombosis and microbial infection. (2) Studied the blood coagulation response and Bacterial Adhesion to such biomimetic PU surfaces, and demonstrated that the combination of surface texturing and NO release synergistically reduced the platelet Adhesion and Bacterial Adhesion in plasma, providing an effective approach to improve the biocompatibility of biomaterials used in blood-contacting medical devices. (3) The NO releasing surface significantly inhibits the plasma coagulation via the reduction of contact activation of FXII, indicating the multifunctional roles of NO in improving the biocompatibility of biomaterials in blood-contacting medical devices.

  • inhibition of Bacterial Adhesion and biofilm formation by dual functional textured and nitric oxide releasing surfaces
    Acta Biomaterialia, 2017
    Co-Authors: Mark E. Meyerhoff, Christopher A Siedlecki
    Abstract:

    Abstract In separate prior studies, physical topographic surface modification or nitric oxide (NO) release has been demonstrated to each be an effective approach to inhibit and control Bacterial Adhesion and biofilm formation on polymeric surfaces. Such approaches can prevent biomaterial-associated infection without causing the antibiotic resistance of the strain. In this work, both techniques were successfully integrated and applied to a polyurethane (PU) biomaterial surface that bears ordered pillar topographies (400/400 nm and 500/500 nm patterns) at the top surface and a S -nitroso- N -acetylpenicillamine (SNAP, NO donor) doped sub-layer in the middle, via a soft lithography two-stage replication process. Upon placing the SNAP textured PU films into PBS at 37 °C, the decomposition of SNAP within polymer film initiates NO release with a lifetime of up to 10 days at flux levels >0.5 × 10 −10  mol min −1  cm −2 for a textured polyurethane layer containing 15 wt% SNAP. The textured surface reduces the accessible surface area and the opportunity of bacteria-surface interaction, while the NO release from the same surface further inhibits Bacterial growth and biofilm formation. Such dual functionality surfaces are shown to provide a synergistic effect on inhibition of Staphylococcus epidermidis Bacterial Adhesion that is significantly greater than the inhibition of Bacterial Adhesion achieved by either single treatment approach alone. Longer term experiments to observe biofilm formation demonstrate that the SNAP doped-textured PU surface can inhibit the biofilm formation for >28 d and provide a practical approach to improve the biocompatibility of current biomimetic biomaterials and thereby reduce the risk of pathogenic infection. Statement of Significance Microbial infection remains a significant barrier to development and implementation of advanced blood-contacting medical devices. Clearly, determining how to design and control material properties that can reduce microbial infection is a central question to biomaterial researchers. In separate prior studies, physical topographic surface modification or nitric oxide (NO) release has been demonstrated to each be an effective approach to inhibit and control Bacterial Adhesion and biofilm formation on polymeric surfaces. Such approaches can prevent biomaterial-associated infection without causing antibiotic resistance of the Bacterial strain. However, efficiency of antimicrobial properties of each approach is still limited and far from sufficient for widespread clinical use. This work successfully integrates both techniques and applies them to a polyurethane (PU) biomaterial surface that bears dual functions, surface topographic modification and NO release. The former reduces the surface contact area and changes surface wettability, resulting in reduction of Bacterial Adhesion, and NO release further inhibits bacteria growth. Such dual functionalized surfaces provide a synergistic effect on inhibition of Staphylococcus epidermidis Bacterial Adhesion that is significantly greater than the inhibition of Bacterial Adhesion achieved by either single treatment approach alone. Furthermore, longer-term experiments demonstrate that the dual functionalized surfaces can inhibit biofilm formation for >28 days. The success of this work provides a practical approach to improve the biocompatibility of current biomaterials and thereby reduce the risk of pathogenic infection.

  • Submicron-textured biomaterial surface reduces staphylococcal Bacterial Adhesion and biofilm formation.
    Acta biomaterialia, 2011
    Co-Authors: Christopher A Siedlecki
    Abstract:

    Abstract Staphylococci are among the most important pathogens causing bloodstream infections associated with implanted medical devices. Control of Bacterial Adhesion to material surfaces is important for prevention of biofilm formation and biomaterial-associated infections. In this study, we hypothesized that submicron (staphylococcal Bacterial dimension) surface textures may reduce the Bacterial Adhesion via a decrease in surface area that bacteria can contact, and subsequently inhibit biofilm formation. Poly(urethane urea) films were textured with two different sizes of submicron pillars via a two-stage replication process. Adhesion of two Bacterial strains ( Staphylococcus epidermidis RP62A and S. aureus Newman) was assessed over a shear stress range of 0–13.2 dyn cm −2 using a rotating disk system in physiological buffer solutions. Significant decreases in Bacterial Adhesion were observed on textured surfaces for both strains compared with smooth controls. Biofilm formation was further tested on surfaces incubated in solution for either 2 or 5 days and it was found that biofilm formation was dramatically inhibited on textured surfaces. The results of the approaches used in this work demonstrate that patterned surface texturing of biomaterials provides an effective means to reduce staphylococcal Adhesion and biofilm formation on biomaterial surfaces, and thus to prevent biomaterial-associated infections.

Jeanpaul Steghens - One of the best experts on this subject based on the ideXlab platform.

Claudia Ludecke - One of the best experts on this subject based on the ideXlab platform.

  • reproducible biofilm cultivation of chemostat grown escherichia coli and investigation of Bacterial Adhesion on biomaterials using a non constant depth film fermenter
    PLOS ONE, 2014
    Co-Authors: Claudia Ludecke, Klaus D Jandt, Daniel Siegismund, Marian J Kujau, Emerson Zang, Markus Rettenmayr, Jorg Bossert, Martin Roth
    Abstract:

    Biomaterials-associated infections are primarily initiated by the Adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial Adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and Bacterial Adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate Bacterial Adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating Bacterial Adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial Adhesion on biomaterials comprised an initial lag-phase I followed by a fast Adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, Adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during Adhesion phase II. This study introduces the non-constant-depth film fermenter in combination with a chemostat culture to allow for a controlled approach to reproducibly cultivate biofilms and to investigate Bacterial Adhesion kinetics at constant and low shear conditions. The findings will support developing and adequate testing of biomaterials surface modifications eventually preventing biomaterial-associated infections.

Martin Roth - One of the best experts on this subject based on the ideXlab platform.

  • Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film
    2016
    Co-Authors: Klaus D. J, Daniel Siegismund, Marian J Kujau, Emerson Zang, Markus Rettenmayr, Martin Roth
    Abstract:

    Biomaterials-associated infections are primarily initiated by the Adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial Adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and Bacterial Adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate Bacterial Adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating Bacterial Adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial Adhesion on biomaterials comprised an initial lag-phase I followed by a fast Adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, Adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during Adhesion phase II. This study introduces the non-constant-depth film fermenter i

  • reproducible biofilm cultivation of chemostat grown escherichia coli and investigation of Bacterial Adhesion on biomaterials using a non constant depth film fermenter
    PLOS ONE, 2014
    Co-Authors: Claudia Ludecke, Klaus D Jandt, Daniel Siegismund, Marian J Kujau, Emerson Zang, Markus Rettenmayr, Jorg Bossert, Martin Roth
    Abstract:

    Biomaterials-associated infections are primarily initiated by the Adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial Adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and Bacterial Adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate Bacterial Adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating Bacterial Adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial Adhesion on biomaterials comprised an initial lag-phase I followed by a fast Adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, Adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during Adhesion phase II. This study introduces the non-constant-depth film fermenter in combination with a chemostat culture to allow for a controlled approach to reproducibly cultivate biofilms and to investigate Bacterial Adhesion kinetics at constant and low shear conditions. The findings will support developing and adequate testing of biomaterials surface modifications eventually preventing biomaterial-associated infections.

Related terms

Bacterial Adhesion - 15 days free trial to Access Experts & Abstracts