Bacterial Antagonist

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Chenghua Li - One of the best experts on this subject based on the ideXlab platform.

  • vibrio sp 33 a potential Bacterial Antagonist of vibrio splendidus pathogenic to sea cucumber apostichopus japonicus
    Aquaculture, 2017
    Co-Authors: Shanshan Zhang, Weiwei Zhang, Chenghua Li
    Abstract:

    Abstract Recently, the use of probiotics as an alternative strategy to control Bacterial diseases in aquaculture has garnered much attention due to the merits of environmental friendliness. In this study, a bacterium identified as Vibrio sp. V33 was isolated from healthy sepia and was determined to have strong Antagonistic activity towards the pathogenic isolate Vibrio splendidus Vs using the disk diffusion method. The Antagonistic substance was secreted into the supernatant and was thermo-stable. The Antagonistic activity of Vibrio sp. V33 may be attributed to its higher ability to compete for iron than that of V . splendidus Vs, as deduced from siderophore quantification using the CAS method. Number of colony counts from seawater and Apostichopus japonicus infection experiments showed that Vibrio sp. V33 significantly decreased the quantity of V . splendidus Vs in natural seawater and promoted the survival rate of A . japonicus infected by V . splendidus , with a relative percentage survival of 43% under the current conditions. Our findings suggest that V33 could be potentially used as potential Bacterial Antagonist for controlling V . splendidus infections in sea cucumber aquaculture. Statement of relevance It might protect A . japonicus from V . splendidus infection.

Apinya Jantharangsri - One of the best experts on this subject based on the ideXlab platform.

  • floating pellets containing Bacterial Antagonist for control sheath blight of rice formulations viability and Bacterial release studies
    Journal of Controlled Release, 2004
    Co-Authors: Ruedeekorn Wiwattanapatapee, Ashara Pengnoo, Mana Kanjanamaneesathian, Wilairat Matchavanich, Ladda Nilratana, Apinya Jantharangsri
    Abstract:

    Abstract Floating pellets containing spores of Bacterial biological control agent, Bacillus megaterium were prepared by extrusion–spheronization process. The formulations composed of hydrogenated vegetable oil (HVO), lactose, microcrystalline cellulose (Avicel® PH101), and a disintegrant; cross-linked sodium carboxymethylcellulose (Ac-Di-Sol®). The finishing pellets contained bacteria ranging from 107 to 108 CFU/g and the viability of bacteria in all formulations remained high after 6 months storage. The scanning electron microscope (SEM) was used to observe endospores of B. megaterium on both the surface and the inside of the pellets. The formulations were tested for their physical properties, floating ability and Bacterial release. The level of disintegrant in the formulations influenced the floating ability and the liberation of Antagonistic bacteria from pellets. The Bacterial pellets showed promising result in suppression of the development of sheath blight lesions in greenhouse experiment.

Manoj A Kumar - One of the best experts on this subject based on the ideXlab platform.

  • pseudomonas fluorescens a potential Bacterial Antagonist to control plant diseases
    Journal of Plant Interactions, 2005
    Co-Authors: Girija Ganeshan, Manoj A Kumar
    Abstract:

    Abstract Fluorescent Pseudomonads belong to plant Growth Promoting Rhizobacteria (PGPR), the important group of bacteria that play a major role in the plant growth promotion, induced systemic resistance, biological control of pathogens etc. Many strains of Pseudomonas fluorescens are known to enhance plant growth promotion and reduce severity of various diseases. The efficacy of Bacterial Antagonists in controlling fungal diseases was often better as alone, and sometimes in combination with fungicides. The present review refers to occurrence, distribution, mechanism, growth requirements of P. fluorescens and diseases controlled by the Bacterial Antagonist in different agricultural and horticultural crops were discussed. The literature in this review helps in future research programmes that aim to promote P. fluorescens as a potential bio-pesticide for augmentative biological control of many diseases of agriculture and horticultural importance.

Mana Kanjanamaneesathian - One of the best experts on this subject based on the ideXlab platform.

  • Bacterial Antagonist as Seed Treatment to Control Leaf Blight Disease of Bambara Groundnut (Vigna subterranea)
    World Journal of Microbiology and Biotechnology, 2006
    Co-Authors: Ashara Pengnoo, Ruedeekorn Wiwattanapattapee, Amornrat Chumthong, Mana Kanjanamaneesathian
    Abstract:

    SummaySoil samples were taken from 48 fields in the southern part of Thailand in which either bambara groundnut (Vigna subterranea) or groundnut (Arachis hypogeae) had been planted. Bacillus spp. were isolated using soil dilution plates and heat treatment to screen for endospore-producing bacteria. Among 342 Bacillus spp. isolates tested, 168 isolates were not Antagonistic to Bradyrhizobium sp. strain NC-92 using dual culture technique. Further testing found 16 isolates of Bacillus spp. had the ability to inhibit mycelial growth of Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut. Among these isolates, Bacillus spp. isolate TRV 9-5-2 had the greatest activity in anti-microbial tests against R. solani. This isolate was later identified as B. firmus. A powder formulation of B. firmus was developed by mixing Bacterial endospores, talcum, sodium carboxymethylcellulose (SCMC) and polyvinylpyrolidone (PVP). The formulations contained Bacterial levels ranging from 108 to 1010 c.f.u./g and the viability of bacteria in all formulations remained high after 1 year storage at room temperature (26–32 °C). All formulations showed satisfactory effectiveness in vitro in suppressing mycelial growth of R. solani using dual culture technique. The application of formulations as seed treatment showed that these formulations did not cause abnormality of seedling shape and had no effect on the germination of bambara groundnut seeds.

  • floating pellets containing Bacterial Antagonist for control sheath blight of rice formulations viability and Bacterial release studies
    Journal of Controlled Release, 2004
    Co-Authors: Ruedeekorn Wiwattanapatapee, Ashara Pengnoo, Mana Kanjanamaneesathian, Wilairat Matchavanich, Ladda Nilratana, Apinya Jantharangsri
    Abstract:

    Abstract Floating pellets containing spores of Bacterial biological control agent, Bacillus megaterium were prepared by extrusion–spheronization process. The formulations composed of hydrogenated vegetable oil (HVO), lactose, microcrystalline cellulose (Avicel® PH101), and a disintegrant; cross-linked sodium carboxymethylcellulose (Ac-Di-Sol®). The finishing pellets contained bacteria ranging from 107 to 108 CFU/g and the viability of bacteria in all formulations remained high after 6 months storage. The scanning electron microscope (SEM) was used to observe endospores of B. megaterium on both the surface and the inside of the pellets. The formulations were tested for their physical properties, floating ability and Bacterial release. The level of disintegrant in the formulations influenced the floating ability and the liberation of Antagonistic bacteria from pellets. The Bacterial pellets showed promising result in suppression of the development of sheath blight lesions in greenhouse experiment.

Shanshan Zhang - One of the best experts on this subject based on the ideXlab platform.

  • vibrio sp 33 a potential Bacterial Antagonist of vibrio splendidus pathogenic to sea cucumber apostichopus japonicus
    Aquaculture, 2017
    Co-Authors: Shanshan Zhang, Weiwei Zhang, Chenghua Li
    Abstract:

    Abstract Recently, the use of probiotics as an alternative strategy to control Bacterial diseases in aquaculture has garnered much attention due to the merits of environmental friendliness. In this study, a bacterium identified as Vibrio sp. V33 was isolated from healthy sepia and was determined to have strong Antagonistic activity towards the pathogenic isolate Vibrio splendidus Vs using the disk diffusion method. The Antagonistic substance was secreted into the supernatant and was thermo-stable. The Antagonistic activity of Vibrio sp. V33 may be attributed to its higher ability to compete for iron than that of V . splendidus Vs, as deduced from siderophore quantification using the CAS method. Number of colony counts from seawater and Apostichopus japonicus infection experiments showed that Vibrio sp. V33 significantly decreased the quantity of V . splendidus Vs in natural seawater and promoted the survival rate of A . japonicus infected by V . splendidus , with a relative percentage survival of 43% under the current conditions. Our findings suggest that V33 could be potentially used as potential Bacterial Antagonist for controlling V . splendidus infections in sea cucumber aquaculture. Statement of relevance It might protect A . japonicus from V . splendidus infection.