The Experts below are selected from a list of 120 Experts worldwide ranked by ideXlab platform
Dean C. Ripple - One of the best experts on this subject based on the ideXlab platform.
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Evaluating changes to Ralstonia pickettii in high-purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii , was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (
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evaluating changes to ralstonia pickettii in high purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:: Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii, was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (< 1 µm diameter) and dim, while covering a broad emission range from ≈ (420 to 600) nm to best mimic the representative R. pickettii.
Nurul Adilla Mohd Subha - One of the best experts on this subject based on the ideXlab platform.
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Analysis of Bacterial Contaminant in Pasir Gudang, Johor Tap Water Supply–Varies pH Value Observation
International Journal of Engineering, 2018Co-Authors: Syahidah Nurani Zulkifli, Herlina Abdul Rahim, Nurul Adilla Mohd SubhaAbstract:The number of breakthrough pathogenic activity in water distribution network system is constantly increasing day by day especially at level of consumption. Bacterial growth or survival rate often relates to acidity and alkalinity of water. Sudden changes in pH value and temperature indicates a possibility of present Bacterial Contaminant in aqueous environment. The observation of pH- and temperature-based for tap water supply samples in Pasir Gudang regions therefore was determined. On the basis of the findings, the observed pH value was compared to the recommended range for pH tap and drinking water, which is between 6.5-8.5. A significant spread can be seen among the measured parameters within the range of pH and temperature at 6.00 to 8.65 and 19.20 to 32.00 °C, respectively. There is a statistically significant difference between each sampling regions based on the measured pH value ( 44.79, determined by one-way ANOVA. The pH value and temperature evidence a significant effects by the location of tap water samples near industrial regions. There also appears to be a negative Pearson correlation between the two water parameters in four out of five regions.
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analysis of Bacterial Contaminant in pasir gudang johor tap water supply varies ph value observation
International Journal of Engineering, 2018Co-Authors: Syahidah Nurani Zulkifli, Herlina Abdul Rahim, Nurul Adilla Mohd SubhaAbstract:The number of breakthrough pathogenic activity in water distribution network system is constantly increasing day by day especially at level of consumption. Bacterial growth or survival rate often relates to acidity and alkalinity of water. Sudden changes in pH value and temperature indicates a possibility of present Bacterial Contaminant in aqueous environment. The observation of pH- and temperature-based for tap water supply samples in Pasir Gudang regions therefore was determined. On the basis of the findings, the observed pH value was compared to the recommended range for pH tap and drinking water, which is between 6.5-8.5. A significant spread can be seen among the measured parameters within the range of pH and temperature at 6.00 to 8.65 and 19.20 to 32.00 °C, respectively. There is a statistically significant difference between each sampling regions based on the measured pH value ( 44.79, determined by one-way ANOVA. The pH value and temperature evidence a significant effects by the location of tap water samples near industrial regions. There also appears to be a negative Pearson correlation between the two water parameters in four out of five regions.
Kurt D. Benkstein - One of the best experts on this subject based on the ideXlab platform.
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Evaluating changes to Ralstonia pickettii in high-purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii , was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (
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evaluating changes to ralstonia pickettii in high purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:: Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii, was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (< 1 µm diameter) and dim, while covering a broad emission range from ≈ (420 to 600) nm to best mimic the representative R. pickettii.
Syahidah Nurani Zulkifli - One of the best experts on this subject based on the ideXlab platform.
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Analysis of Bacterial Contaminant in Pasir Gudang, Johor Tap Water Supply–Varies pH Value Observation
International Journal of Engineering, 2018Co-Authors: Syahidah Nurani Zulkifli, Herlina Abdul Rahim, Nurul Adilla Mohd SubhaAbstract:The number of breakthrough pathogenic activity in water distribution network system is constantly increasing day by day especially at level of consumption. Bacterial growth or survival rate often relates to acidity and alkalinity of water. Sudden changes in pH value and temperature indicates a possibility of present Bacterial Contaminant in aqueous environment. The observation of pH- and temperature-based for tap water supply samples in Pasir Gudang regions therefore was determined. On the basis of the findings, the observed pH value was compared to the recommended range for pH tap and drinking water, which is between 6.5-8.5. A significant spread can be seen among the measured parameters within the range of pH and temperature at 6.00 to 8.65 and 19.20 to 32.00 °C, respectively. There is a statistically significant difference between each sampling regions based on the measured pH value ( 44.79, determined by one-way ANOVA. The pH value and temperature evidence a significant effects by the location of tap water samples near industrial regions. There also appears to be a negative Pearson correlation between the two water parameters in four out of five regions.
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analysis of Bacterial Contaminant in pasir gudang johor tap water supply varies ph value observation
International Journal of Engineering, 2018Co-Authors: Syahidah Nurani Zulkifli, Herlina Abdul Rahim, Nurul Adilla Mohd SubhaAbstract:The number of breakthrough pathogenic activity in water distribution network system is constantly increasing day by day especially at level of consumption. Bacterial growth or survival rate often relates to acidity and alkalinity of water. Sudden changes in pH value and temperature indicates a possibility of present Bacterial Contaminant in aqueous environment. The observation of pH- and temperature-based for tap water supply samples in Pasir Gudang regions therefore was determined. On the basis of the findings, the observed pH value was compared to the recommended range for pH tap and drinking water, which is between 6.5-8.5. A significant spread can be seen among the measured parameters within the range of pH and temperature at 6.00 to 8.65 and 19.20 to 32.00 °C, respectively. There is a statistically significant difference between each sampling regions based on the measured pH value ( 44.79, determined by one-way ANOVA. The pH value and temperature evidence a significant effects by the location of tap water samples near industrial regions. There also appears to be a negative Pearson correlation between the two water parameters in four out of five regions.
Sandra M. Silva - One of the best experts on this subject based on the ideXlab platform.
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Evaluating changes to Ralstonia pickettii in high-purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii , was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (
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evaluating changes to ralstonia pickettii in high purity water to guide selection of potential calibration materials for online water bioburden analyzers
Journal of Industrial Microbiology & Biotechnology, 2019Co-Authors: Kurt D. Benkstein, Sandra M. Silva, Dean C. RippleAbstract:: Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and Bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant Bacterial Contaminant, Ralstonia pickettii, was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (< 1 µm diameter) and dim, while covering a broad emission range from ≈ (420 to 600) nm to best mimic the representative R. pickettii.