The Experts below are selected from a list of 271161 Experts worldwide ranked by ideXlab platform
Kaifeng Zhou - One of the best experts on this subject based on the ideXlab platform.
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adenosine 5 triphosphate atp protects mice against Bacterial Infection by activation of the nlrp3 inflammasome
PLOS ONE, 2013Co-Authors: Yang Xiang, Xuan Wang, Chao Yan, Qian Gao, Jie Liu, Kaifeng ZhouAbstract:It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during Bacterial Infection. By using a murine peritonitis model, we also found that ATP promotes the fight against Bacterial Infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against Bacterial Infection. In the present study, we demonstrated a protective role for ATP during Bacterial Infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from Infections.
Xuan Wang - One of the best experts on this subject based on the ideXlab platform.
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adenosine 5 triphosphate atp protects mice against Bacterial Infection by activation of the nlrp3 inflammasome
PLOS ONE, 2013Co-Authors: Yang Xiang, Xuan Wang, Chao Yan, Qian Gao, Jie Liu, Kaifeng ZhouAbstract:It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during Bacterial Infection. By using a murine peritonitis model, we also found that ATP promotes the fight against Bacterial Infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against Bacterial Infection. In the present study, we demonstrated a protective role for ATP during Bacterial Infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from Infections.
Chao Yan - One of the best experts on this subject based on the ideXlab platform.
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adenosine 5 triphosphate atp protects mice against Bacterial Infection by activation of the nlrp3 inflammasome
PLOS ONE, 2013Co-Authors: Yang Xiang, Xuan Wang, Chao Yan, Qian Gao, Jie Liu, Kaifeng ZhouAbstract:It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during Bacterial Infection. By using a murine peritonitis model, we also found that ATP promotes the fight against Bacterial Infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against Bacterial Infection. In the present study, we demonstrated a protective role for ATP during Bacterial Infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from Infections.
Jie Liu - One of the best experts on this subject based on the ideXlab platform.
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adenosine 5 triphosphate atp protects mice against Bacterial Infection by activation of the nlrp3 inflammasome
PLOS ONE, 2013Co-Authors: Yang Xiang, Xuan Wang, Chao Yan, Qian Gao, Jie Liu, Kaifeng ZhouAbstract:It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, the physiological effect of extracellular ATP on NLRP3 inflammasome activation has not yet been investigated. In the present study, we found that ATP was indeed released during Bacterial Infection. By using a murine peritonitis model, we also found that ATP promotes the fight against Bacterial Infection in mice. ATP induced the secretion of IL-1β and chemokines by murine bone marrow-derived macrophages in vitro. Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1β and chemokines in the mouse peritoneal lavage. Neutrophils were rapidly recruited to the peritoneum after ATP injection. In addition, the effects on cytokine and chemokine secretion and neutrophil recruitment were markedly attenuated by the pre-administration of the caspase-1 inhibitor Ac-YVAD-cho. Ac-YVAD-cho also significantly attenuated the protective effect of ATP against Bacterial Infection. In the present study, we demonstrated a protective role for ATP during Bacterial Infection and this effect was related to NLRP3 inflammasome activation. Together, these results suggest a role for ATP in initiating the immune response in hosts suffering from Infections.
Martin Chalumeau - One of the best experts on this subject based on the ideXlab platform.
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why children with severe Bacterial Infection die a population based study of determinants and consequences of suboptimal care with a special emphasis on methodological issues
PLOS ONE, 2014Co-Authors: Elise Launay, Christele Grasle Guen, Alain Martinot, Remi Assathiany, Thomas Blanchais, Jeanchristophe Roze, Martin Chalumeau, Elise Martin, Catherine DeneuxtharauxAbstract:Introduction Suboptimal care is frequent in the management of severe Bacterial Infection. We aimed to evaluate the consequences of suboptimal care in the early management of severe Bacterial Infection in children and study the determinants. Methods A previously reported population-based confidential enquiry included all children (3 months- 16 years) who died of severe Bacterial Infection in a French area during a 7-year period. Here, we compared the optimality of the management of these cases to that of pediatric patients who survived a severe Bacterial Infection during the same period for 6 types of care: seeking medical care by parents, evaluation of sepsis signs and detection of severe disease by a physician, timing and dosage of antibiotic therapy, and timing and dosage of saline bolus. Two independent experts blinded to outcome and final diagnosis evaluated the optimality of these care types. The effect of suboptimal care on survival was analyzed by a logistic regression adjusted on confounding factors identified by a causal diagram. Determinants of suboptimal care were analyzed by multivariate multilevel logistic regression. Results Suboptimal care was significantly more frequent during early management of the 21 children who died as compared with the 93 survivors: 24% vs 13% (p = 0.003). The most frequent suboptimal care types were delay to seek medical care (20%), under-evaluation of severity by the physician (20%) and delayed antibiotic therapy (24%). Young age (under 1 year) was independently associated with higher risk of suboptimal care, whereas being under the care of a paediatric emergency specialist or a mobile medical unit as compared with a general practitioner was associated with reduced risk. Conclusions Suboptimal care in the early management of severe Bacterial Infection had a global independent negative effect on survival. Suboptimal care may be avoided by better training of primary care physicians in the specifics of pediatric medicine.
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suboptimal care in the initial management of children who died from severe Bacterial Infection a population based confidential inquiry
Pediatric Critical Care Medicine, 2010Co-Authors: Elise Launay, Christele Grasle Guen, Alain Martinot, Remi Assathiany, Thomas Blanchais, Nadjette Mourdi, Albertine Aouba, M H Bouviercolle, Jeanchristophe Roze, Martin ChalumeauAbstract:OBJECTIVES To study the frequency and types of suboptimal care and medical errors in children who died of severe Bacterial Infection as the first-stage procedure intended to improve quality of care. DESIGN Population-based confidential inquiry. SETTING Two adjoining administrative districts in France. PATIENTS Children older than 3 months dead from severe Bacterial Infection from 2000 through 2006. INTERVENTIONS The medical files were summarized on standardized forms and then evaluated independently by two experts, who determined whether the initial management before the patients' arrival in intensive care was or was not optimal, in comparison with current guidelines. MEASUREMENTS AND MAIN RESULTS Of 23 deaths from severe Bacterial Infection, 21 could be analyzed; management was considered suboptimal in 76%. The coefficient of agreement between the experts was high, with a weighted kappa of 0.73. The types of errors identified included parental delay in seeking medical care (33%; 95% confidence interval, [12-54]), physicians' delay in administering appropriate treatment (antibiotic therapy in the case of purpura; 38%; 95% confidence interval, 16-60), insufficient doses of or failure to repeat fluid resuscitation (24%; 95% confidence interval, [9 -35]), and overall underestimation of disease severity (38%; 95% confidence interval, [16-60]). CONCLUSION This study found a high frequency of suboptimal care in the initial management of children who died of severe Bacterial Infection, with four separate types of errors. Other studies are needed to assess the potential avoidability of this type of death.