The Experts below are selected from a list of 107901 Experts worldwide ranked by ideXlab platform
Congyang Mao - One of the best experts on this subject based on the ideXlab platform.
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local photothermal photodynamic synergistic therapy by disrupting Bacterial Membrane to accelerate reactive oxygen species permeation and protein leakage
ACS Applied Materials & Interfaces, 2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin LiangAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats' backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques.
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Local Photothermal/Photodynamic Synergistic Therapy by Disrupting Bacterial Membrane To Accelerate Reactive Oxygen Species Permeation and Protein Leakage
2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin Liang, Shengli ZhuAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats’ backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques
Yanqin Liang - One of the best experts on this subject based on the ideXlab platform.
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local photothermal photodynamic synergistic therapy by disrupting Bacterial Membrane to accelerate reactive oxygen species permeation and protein leakage
ACS Applied Materials & Interfaces, 2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin LiangAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats' backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques.
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Local Photothermal/Photodynamic Synergistic Therapy by Disrupting Bacterial Membrane To Accelerate Reactive Oxygen Species Permeation and Protein Leakage
2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin Liang, Shengli ZhuAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats’ backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques
Yiming Xiang - One of the best experts on this subject based on the ideXlab platform.
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local photothermal photodynamic synergistic therapy by disrupting Bacterial Membrane to accelerate reactive oxygen species permeation and protein leakage
ACS Applied Materials & Interfaces, 2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin LiangAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats' backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques.
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Local Photothermal/Photodynamic Synergistic Therapy by Disrupting Bacterial Membrane To Accelerate Reactive Oxygen Species Permeation and Protein Leakage
2019Co-Authors: Congyang Mao, Yiming Xiang, Xiangmei Liu, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Xianjin Yang, Yanqin Liang, Shengli ZhuAbstract:Bacterial infection is still a ticklish clinical challenge even though some advanced antiBacterial materials and techniques have been put forward. This work reports that rapid and effective antiBacterial performance is achieved by the synergistic local photothermal and photodynamic therapy (PTDT). Within 10 min of light irradiation, both Escherichia coli and Staphylococcus aureus are almost completely eliminated by the action of photothermy (52.1 °C) and limited reactive oxygen species (ROS), the corresponding Bacterial killing efficiencies are 99.91 and 99.97%, respectively, which are far higher than single modal therapy, i.e., photothermal therapy or photodynamic therapy with antiBacterial efficacy of 50 or 70%, respectively. The mechanism is that Bacterial Membrane permeation is increased by PTDT because photothermy shows more severe impact only on E. coli by destroying the outmost Bacterial panniculus, whereas the inner panniculus of the two kinds of bacteria is more sensitive to ROS. Hence, ROS penetrates the Bacterial Membrane more easily, and meanwhile, the proteins in the bacteria are severely lost after the Bacterial Membrane disruption, which leads to Bacterial death. In vivo results reveal that rapid and effective sterilization is an important process to accelerate wound healing, and the traumas on the rats’ backbones heal well within 12 days by PTDT. Furthermore, the PTDT is friendly to major organs of rats during the therapeutic process. Therefore, the synergistic therapy system can be a safe therapeutic system for clinical sterilization with great potential. More importantly, the antiBacterial mechanism presented in this work has great guiding significance for the design of other advanced antiBacterial systems and techniques
Riaz Akseer - One of the best experts on this subject based on the ideXlab platform.
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lavender essential oil induces oxidative stress which modifies the Bacterial Membrane permeability of carbapenemase producing klebsiella pneumoniae
Scientific Reports, 2020Co-Authors: Shunkai Yang, Riaz Akseer, Khatijah Yusoff, Warren Thomas, Maryam Sultan Alhosani, Aisha AbushelaibiAbstract:Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropenem in suppressing the growth of carbapenemase-producing K. pneumoniae (KPC-KP). Synergistic interactions between LVO and meropenem were detected, which significantly reduce the inhibitory concentration of both LVO and meropenem by 15 and 4-fold respectively. Comparative proteomic profiling identified a disruption in the Bacterial Membrane via oxidative stress that was indicated by loss of Membrane and cytoplasmic proteins and the upregulation of oxidative regulators. As a proof of concept, zeta potential measurements showed a change in cell surface charge while outer Membrane permeability measurement indicated an increase in Membrane permeability following exposure to LVO. This was indicative of a disrupted outer Membrane. Ethidium bromide influx/efflux assays demonstrated no significant efflux pump inhibition by LVO, and scanning electron microscopy revealed irregularities on the cell surface after exposure to LVO. Oxidative stress was also detected with increased level of ROS and lipid peroxidation in LVO-treated cells. In conclusion, our data suggest that LVO induced oxidative stress in K. pneumoniae which oxidizes the outer Membrane, enabling the influx of generated ROS, LVO and meropenem into the Bacterial cells, causing damage to the cells and eventually death.
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disruption of kpc producing klebsiella pneumoniae Membrane via induction of oxidative stress by cinnamon bark cinnamomum verum j presl essential oil
PLOS ONE, 2019Co-Authors: Shunkai Yang, Mokrish Ajat, Aisha Abushelaibi, Khatijah Yusoff, Warren Thomas, Riaz AkseerAbstract:Klebsiella pneumoniae (KP) remains the most prevalent nosocomial pathogen and carries the carbapenemase (KPC) gene which confers resistance towards carbapenem. Thus, it is necessary to discover novel antimicrobials to address the issue of antimicrobial resistance in such pathogens. Natural products such as essential oils are a promising source due to their complex composition. Essential oils have been shown to be effective against pathogens, but the overall mechanisms have yet to be fully explained. Understanding the molecular mechanisms of essential oil towards KPC-KP cells would provide a deeper understanding of their potential use in clinical settings. Therefore, we aimed to investigate the mode of action of essential oil against KPC-KP cells from a proteomic perspective by comparing the overall proteome profile of KPC-KP cells treated with cinnamon bark (Cinnamomum verum J. Presl) essential oil (CBO) at their sub-inhibitory concentration of 0.08% (v/v). A total of 384 proteins were successfully identified from the non-treated cells, whereas only 242 proteins were identified from the CBO-treated cells. Proteins were then categorized based on their biological processes, cellular components and molecular function prior to pathway analysis. Pathway analysis showed that CBO induced oxidative stress in the KPC-KP cells as indicated by the abundance of oxidative stress regulator proteins such as glycyl radical cofactor, catalase peroxidase and DNA mismatch repair protein. Oxidative stress is likely to oxidize and disrupt the Bacterial Membrane as shown by the loss of major Membrane proteins. Several genes selected for qRT-PCR analysis validated the proteomic profile and were congruent with the proteomic abundance profiles. In conclusion, KPC-KP cells exposed to CBO undergo oxidative stress that eventually disrupts the Bacterial Membrane possibly via interaction with the phospholipid bilayer. Interestingly, several pathways involved in the Bacterial Membrane repair system were also affected by oxidative stress, contributing to the loss of cells viability.
Aisha Abushelaibi - One of the best experts on this subject based on the ideXlab platform.
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lavender essential oil induces oxidative stress which modifies the Bacterial Membrane permeability of carbapenemase producing klebsiella pneumoniae
Scientific Reports, 2020Co-Authors: Shunkai Yang, Riaz Akseer, Khatijah Yusoff, Warren Thomas, Maryam Sultan Alhosani, Aisha AbushelaibiAbstract:Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropenem in suppressing the growth of carbapenemase-producing K. pneumoniae (KPC-KP). Synergistic interactions between LVO and meropenem were detected, which significantly reduce the inhibitory concentration of both LVO and meropenem by 15 and 4-fold respectively. Comparative proteomic profiling identified a disruption in the Bacterial Membrane via oxidative stress that was indicated by loss of Membrane and cytoplasmic proteins and the upregulation of oxidative regulators. As a proof of concept, zeta potential measurements showed a change in cell surface charge while outer Membrane permeability measurement indicated an increase in Membrane permeability following exposure to LVO. This was indicative of a disrupted outer Membrane. Ethidium bromide influx/efflux assays demonstrated no significant efflux pump inhibition by LVO, and scanning electron microscopy revealed irregularities on the cell surface after exposure to LVO. Oxidative stress was also detected with increased level of ROS and lipid peroxidation in LVO-treated cells. In conclusion, our data suggest that LVO induced oxidative stress in K. pneumoniae which oxidizes the outer Membrane, enabling the influx of generated ROS, LVO and meropenem into the Bacterial cells, causing damage to the cells and eventually death.
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disruption of kpc producing klebsiella pneumoniae Membrane via induction of oxidative stress by cinnamon bark cinnamomum verum j presl essential oil
PLOS ONE, 2019Co-Authors: Shunkai Yang, Mokrish Ajat, Aisha Abushelaibi, Khatijah Yusoff, Warren Thomas, Riaz AkseerAbstract:Klebsiella pneumoniae (KP) remains the most prevalent nosocomial pathogen and carries the carbapenemase (KPC) gene which confers resistance towards carbapenem. Thus, it is necessary to discover novel antimicrobials to address the issue of antimicrobial resistance in such pathogens. Natural products such as essential oils are a promising source due to their complex composition. Essential oils have been shown to be effective against pathogens, but the overall mechanisms have yet to be fully explained. Understanding the molecular mechanisms of essential oil towards KPC-KP cells would provide a deeper understanding of their potential use in clinical settings. Therefore, we aimed to investigate the mode of action of essential oil against KPC-KP cells from a proteomic perspective by comparing the overall proteome profile of KPC-KP cells treated with cinnamon bark (Cinnamomum verum J. Presl) essential oil (CBO) at their sub-inhibitory concentration of 0.08% (v/v). A total of 384 proteins were successfully identified from the non-treated cells, whereas only 242 proteins were identified from the CBO-treated cells. Proteins were then categorized based on their biological processes, cellular components and molecular function prior to pathway analysis. Pathway analysis showed that CBO induced oxidative stress in the KPC-KP cells as indicated by the abundance of oxidative stress regulator proteins such as glycyl radical cofactor, catalase peroxidase and DNA mismatch repair protein. Oxidative stress is likely to oxidize and disrupt the Bacterial Membrane as shown by the loss of major Membrane proteins. Several genes selected for qRT-PCR analysis validated the proteomic profile and were congruent with the proteomic abundance profiles. In conclusion, KPC-KP cells exposed to CBO undergo oxidative stress that eventually disrupts the Bacterial Membrane possibly via interaction with the phospholipid bilayer. Interestingly, several pathways involved in the Bacterial Membrane repair system were also affected by oxidative stress, contributing to the loss of cells viability.