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David A Mills - One of the best experts on this subject based on the ideXlab platform.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Author(s): Locascio, Riccardo G; Ninonuevo, Milady R; Kronewitter, Scott R; Freeman, Samara L; German, J Bruce; Lebrilla, Carlito B; Mills, David A | Abstract: Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.

Kenneth A Cornell - One of the best experts on this subject based on the ideXlab platform.

  • methylthioadenosine s adenosylhomocysteine nucleosidase a critical enzyme for Bacterial Metabolism
    Molecular Microbiology, 2011
    Co-Authors: Nikhat Parveen, Kenneth A Cornell
    Abstract:

    The importance of Methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine through S-adenosylmethionine (SAM)-mediated methylation reactions, and also produces the universal quorum-sensing signal, autoinducer-2 (AI-2). SAM is also essential for synthesis of polyamines, N-acylhomoserine lactone (autoinducer-1), and production of vitamins and other biomolecules formed by SAM radical reactions. MTA, SAH and 5′-deoxyadenosine (5′dADO) are product inhibitors of these reactions, and are substrates of MTA/SAH nucleosidase, underscoring its importance in a wide array of metabolic reactions. Inhibition of this enzyme by certain substrate analogs also limits synthesis of autoinducers, and hence, causes reduction in biofilm formation and may attenuate virulence. Interestingly, the inhibitors of MTA/SAH nucleosidase are very effective against the Lyme disease causing spirochete, Borrelia burgdorferi, which uniquely expresses three homologous functional enzymes. These results indicate that inhibition of this enzyme can affect growth of different bacteria by affecting different mechanisms. Therefore, new inhibitors are currently being explored for development of potential novel broad-spectrum antimicrobials.

  • methylthioadenosine s adenosylhomocysteine nucleosidase a critical enzyme for Bacterial Metabolism
    Molecular Microbiology, 2011
    Co-Authors: Nikhat Parveen, Kenneth A Cornell
    Abstract:

    The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine through S-adenosylmethionine (SAM)-mediated methylation reactions, and also produces the universal quorum-sensing signal, autoinducer-2 (AI-2). SAM is also essential for synthesis of polyamines, N-acylhomoserine lactone (autoinducer-1), and production of vitamins and other biomolecules formed by SAM radical reactions. MTA, SAH and 5'-deoxyadenosine (5'dADO) are product inhibitors of these reactions, and are substrates of MTA/SAH nucleosidase, underscoring its importance in a wide array of metabolic reactions. Inhibition of this enzyme by certain substrate analogues also limits synthesis of autoinducers and hence causes reduction in biofilm formation and may attenuate virulence. Interestingly, the inhibitors of MTA/SAH nucleosidase are very effective against the Lyme disease causing spirochaete, Borrelia burgdorferi, which uniquely expresses three homologous functional enzymes. These results indicate that inhibition of this enzyme can affect growth of different bacteria by affecting different mechanisms. Therefore, new inhibitors are currently being explored for development of potential novel broad-spectrum antimicrobials.

Riccardo G Locascio - One of the best experts on this subject based on the ideXlab platform.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Author(s): Locascio, Riccardo G; Ninonuevo, Milady R; Kronewitter, Scott R; Freeman, Samara L; German, J Bruce; Lebrilla, Carlito B; Mills, David A | Abstract: Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.

Nikhat Parveen - One of the best experts on this subject based on the ideXlab platform.

  • methylthioadenosine s adenosylhomocysteine nucleosidase a critical enzyme for Bacterial Metabolism
    Molecular Microbiology, 2011
    Co-Authors: Nikhat Parveen, Kenneth A Cornell
    Abstract:

    The importance of Methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine through S-adenosylmethionine (SAM)-mediated methylation reactions, and also produces the universal quorum-sensing signal, autoinducer-2 (AI-2). SAM is also essential for synthesis of polyamines, N-acylhomoserine lactone (autoinducer-1), and production of vitamins and other biomolecules formed by SAM radical reactions. MTA, SAH and 5′-deoxyadenosine (5′dADO) are product inhibitors of these reactions, and are substrates of MTA/SAH nucleosidase, underscoring its importance in a wide array of metabolic reactions. Inhibition of this enzyme by certain substrate analogs also limits synthesis of autoinducers, and hence, causes reduction in biofilm formation and may attenuate virulence. Interestingly, the inhibitors of MTA/SAH nucleosidase are very effective against the Lyme disease causing spirochete, Borrelia burgdorferi, which uniquely expresses three homologous functional enzymes. These results indicate that inhibition of this enzyme can affect growth of different bacteria by affecting different mechanisms. Therefore, new inhibitors are currently being explored for development of potential novel broad-spectrum antimicrobials.

  • methylthioadenosine s adenosylhomocysteine nucleosidase a critical enzyme for Bacterial Metabolism
    Molecular Microbiology, 2011
    Co-Authors: Nikhat Parveen, Kenneth A Cornell
    Abstract:

    The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine through S-adenosylmethionine (SAM)-mediated methylation reactions, and also produces the universal quorum-sensing signal, autoinducer-2 (AI-2). SAM is also essential for synthesis of polyamines, N-acylhomoserine lactone (autoinducer-1), and production of vitamins and other biomolecules formed by SAM radical reactions. MTA, SAH and 5'-deoxyadenosine (5'dADO) are product inhibitors of these reactions, and are substrates of MTA/SAH nucleosidase, underscoring its importance in a wide array of metabolic reactions. Inhibition of this enzyme by certain substrate analogues also limits synthesis of autoinducers and hence causes reduction in biofilm formation and may attenuate virulence. Interestingly, the inhibitors of MTA/SAH nucleosidase are very effective against the Lyme disease causing spirochaete, Borrelia burgdorferi, which uniquely expresses three homologous functional enzymes. These results indicate that inhibition of this enzyme can affect growth of different bacteria by affecting different mechanisms. Therefore, new inhibitors are currently being explored for development of potential novel broad-spectrum antimicrobials.

Scott R Kronewitter - One of the best experts on this subject based on the ideXlab platform.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.

  • a versatile and scalable strategy for glycoprofiling bifidoBacterial consumption of human milk oligosaccharides
    Microbial Biotechnology, 2009
    Co-Authors: Riccardo G Locascio, Bruce J German, Milady R Ninonuevo, Samara L Freeman, Carlito B Lebrilla, Scott R Kronewitter, David A Mills
    Abstract:

    Author(s): Locascio, Riccardo G; Ninonuevo, Milady R; Kronewitter, Scott R; Freeman, Samara L; German, J Bruce; Lebrilla, Carlito B; Mills, David A | Abstract: Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of Bacterial Metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of Bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidoBacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C corrections based on de-isotoping protocols. High growth on HMOs was characteristic of Bifidobacterium longum biovar infantis strains, which consumed nearly all available substrates, while other bifidoBacterial strains tested, B. longum bv. longum, B. adolescentis, B. breve and B. bifidum, showed low or only moderate growth ability. Total oligosaccharide consumption ranged from a high of 87% for B. infantis JCM 7009 to only 12% for B. adolescentis ATCC 15703. A detailed analysis of consumption glycoprofiles indicated strain-specific capabilities towards differential Metabolism of milk oligosaccharides. This method overcomes previous limitations in the quantitative, multi-strain analysis of Bacterial Metabolism of HMOs and represents a novel approach towards understanding Bacterial consumption of complex prebiotic oligosaccharides.