The Experts below are selected from a list of 357 Experts worldwide ranked by ideXlab platform

Frank F White - One of the best experts on this subject based on the ideXlab platform.

  • analysis of extreme phenotype bulk copy number variation xp cnv identified the association of rp1 with resistance to goss s wilt of maize
    Frontiers in Plant Science, 2018
    Co-Authors: Ying Hu, Zhao Peng, Arnoldo A Umana, Ha Le, Tatiana V Danilova, Junjie Fu, Haiyan Wang, Alison E Robertson, Scot H Hulbert, Frank F White
    Abstract:

    Goss’s wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout the Great Plains, posing a threat to production. The genetic basis of plant resistance is unknown. Here, a simple method for quantifying disease symptoms was developed and used to select cohorts of highly resistant and highly susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences of bulked XP revealed 141 genes containing copy number variation between the two XP groups. The CNV genes include the previously identified common rust resistant locus rp1. Multiple Rp1 accessions with distinct rp1 haplotypes in an otherwise susceptible accession exhibited hypersensitive responses upon inoculation. GW provides an excellent system for the genetic dissection of a disease, which is caused one of several closely related subspecies of C. michiganesis. Further work will facilitate breeding strategies to control GW and provide needed insight into the important related diseases Bacterial canker of tomato and Bacterial Ring Rot of potato.

Feipeng Wang - One of the best experts on this subject based on the ideXlab platform.

  • laminaria japonica extract an inhibitor of clavibater michiganense subsp sepedonicum
    PLOS ONE, 2014
    Co-Authors: Jia Feng, Feipeng Wang, Qiufeng Xu
    Abstract:

    Bacterial Ring Rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of Bacterial Ring Rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80°C, an extraction time of 12 h, and a solid to liquid ratio of 1∶25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P<0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca2+ concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit pRotein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum.

  • Laminaria japonica Extract, an Inhibitor of Clavibater
    2014
    Co-Authors: Michiganense Subsp Sepedonicum, Jin Cai, Shulian Xie, Jia Feng, Feipeng Wang
    Abstract:

    Bacterial Ring Rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of Bacterial Ring Rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80uC, an extraction time of 12 h, and a solid to liquid ratio of 1:25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P,0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca2+ concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit pRotein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum

  • pRotective effect of polygonum orientale l extracts against clavibater michiganense subsp sepedonicum the causal agent of Bacterial Ring Rot of potato
    PLOS ONE, 2013
    Co-Authors: Jin Cai, Shulian Xie, Jia Feng, Feipeng Wang
    Abstract:

    The Polygonum orientale L. extracts were investigated for antiBacterial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causal agent of a serious disease called Bacterial Ring Rot of potato. The results showed that the leaf extracts of P. orientale had significantly (p<0.05) greater antiBacterial activity against C. michiganense subsp. sepedonicum than root, stem, flower extracts in vitro. According to the results of single factor experiments and L273(13) orthogonal experiments, optimum extraction conditions were A1B3C1, extraction time 6 h, temperature 80°C, solid to liquid ratio 1∶10 (g:mL). The highest (p<0.05) antiBacterial activity was observed when pH was 5, excluding the effect of control. The extracts were stable under ultraviolet (UV). In vivo analysis revealed that 50 mg/mL of P. orientale leaf extracts was effective in controlling decay. Under field conditions, 50 mg/mL of P. orientale leaf extracts also improved growth parameters (whole plant length, shoot length, root length, plant fresh weight, shoot fresh weight, root fresh weight, dry weight, and number of leaves), in the 2010 and 2011 two growing seasons. Further solvent partition assays showed that the most active compounds were in the petroleum ether fractionation. Transmission electron microscopy (TEM) showed drastic ultrastructural changes caused by petroleum ether fractionation, including Bacterial deformation, electron-dense particles, formation of vacuoles and lack of cytoplasmic materials. These results indicated that P. orientale extracts have strong antiBacterial activity against C. michiganense subsp. sepedonicum and a promising effect in control of Bacterial Ring Rot of potato disease.

Ying Hu - One of the best experts on this subject based on the ideXlab platform.

  • analysis of extreme phenotype bulk copy number variation xp cnv identified the association of rp1 with resistance to goss s wilt of maize
    Frontiers in Plant Science, 2018
    Co-Authors: Ying Hu, Zhao Peng, Arnoldo A Umana, Ha Le, Tatiana V Danilova, Junjie Fu, Haiyan Wang, Alison E Robertson, Scot H Hulbert, Frank F White
    Abstract:

    Goss’s wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout the Great Plains, posing a threat to production. The genetic basis of plant resistance is unknown. Here, a simple method for quantifying disease symptoms was developed and used to select cohorts of highly resistant and highly susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences of bulked XP revealed 141 genes containing copy number variation between the two XP groups. The CNV genes include the previously identified common rust resistant locus rp1. Multiple Rp1 accessions with distinct rp1 haplotypes in an otherwise susceptible accession exhibited hypersensitive responses upon inoculation. GW provides an excellent system for the genetic dissection of a disease, which is caused one of several closely related subspecies of C. michiganesis. Further work will facilitate breeding strategies to control GW and provide needed insight into the important related diseases Bacterial canker of tomato and Bacterial Ring Rot of potato.

Scot H Hulbert - One of the best experts on this subject based on the ideXlab platform.

  • analysis of extreme phenotype bulk copy number variation xp cnv identified the association of rp1 with resistance to goss s wilt of maize
    Frontiers in Plant Science, 2018
    Co-Authors: Ying Hu, Zhao Peng, Arnoldo A Umana, Ha Le, Tatiana V Danilova, Junjie Fu, Haiyan Wang, Alison E Robertson, Scot H Hulbert, Frank F White
    Abstract:

    Goss’s wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout the Great Plains, posing a threat to production. The genetic basis of plant resistance is unknown. Here, a simple method for quantifying disease symptoms was developed and used to select cohorts of highly resistant and highly susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences of bulked XP revealed 141 genes containing copy number variation between the two XP groups. The CNV genes include the previously identified common rust resistant locus rp1. Multiple Rp1 accessions with distinct rp1 haplotypes in an otherwise susceptible accession exhibited hypersensitive responses upon inoculation. GW provides an excellent system for the genetic dissection of a disease, which is caused one of several closely related subspecies of C. michiganesis. Further work will facilitate breeding strategies to control GW and provide needed insight into the important related diseases Bacterial canker of tomato and Bacterial Ring Rot of potato.

  • Analysis of Extreme Phenotype Bulk Copy Number Variation (XP-CNV) Identified the Association of rp1 with Resistance to Goss's Wilt of Maize
    Frontiers Media S.A., 2018
    Co-Authors: Jie Ren, Zhao Peng, Arnoldo A Umana, Haiyan Wang, Tatiana Danilova, Alison Robertson, Scot H Hulbert
    Abstract:

    Goss's wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout the Great Plains, posing a threat to production. The genetic basis of plant resistance is unknown. Here, a simple method for quantifying disease symptoms was developed and used to select cohorts of highly resistant and highly susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences of bulked XP revealed 141 genes containing CNV between the two XP groups. The CNV genes include the previously identified common rust resistant locus rp1. Multiple Rp1 accessions with distinct rp1 haplotypes in an otherwise susceptible accession exhibited hypersensitive responses upon inoculation. GW provides an excellent system for the genetic dissection of diseases caused by closely related subspecies of C. michiganesis. Further work will facilitate breeding strategies to control GW and provide needed insight into the resistance mechanism of important related diseases such as Bacterial canker of tomato and Bacterial Ring Rot of potato

Jia Feng - One of the best experts on this subject based on the ideXlab platform.

  • Inhibitory activity of Athyrium sinense extracts against Clavibater michiganense subsp. sepedonicum
    Julius Kühn-Institut, 2015
    Co-Authors: Jin Cai, Jia Feng, Shulian Xie
    Abstract:

    Clavibater michiganense subsp. sepedonicum, the causal agent of a serious disease called Bacterial Ring Rot of potato, is widespread under both greenhouse and field cultivation. In order to find a promising method for the control of this disease, Athyrium sinense extracts were investigated in the study presented here. Results showed that the A. sinense extracts had significantly inhibitory activity against C. michiganense subsp. sepedonicum. Eight organic solvents were used to obtain A. sinense extracts, and ethanol was selected as an ideal extraction solvent. Based on single factor experiment results and L273(13) orthogonal experiment results, optimum extraction conditions were obtained. They were A2B3C3, solid to liquid ratio 1:20 (g:mL), temperature 97°C and extraction time 10h. Stability assays revealed that Zn2+, Fe3+, acidic and neutral conditions enhanced inhibitory activity, and extracts were stable under ultraviolet (UV). Transmission electron microscopy (TEM) showed drastic changes caused by A. sinense extracts, including lack of cytoplasmic materials, formation of vacuoles and Bacterial deformation. These results revealed A. sinense extracts have strong inhibitory activity against C. michiganense subsp. sepedonicum, and can be used as a potent phytochemical fungicide

  • laminaria japonica extract an inhibitor of clavibater michiganense subsp sepedonicum
    PLOS ONE, 2014
    Co-Authors: Jia Feng, Feipeng Wang, Qiufeng Xu
    Abstract:

    Bacterial Ring Rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of Bacterial Ring Rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80°C, an extraction time of 12 h, and a solid to liquid ratio of 1∶25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P<0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca2+ concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit pRotein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum.

  • Laminaria japonica Extract, an Inhibitor of Clavibater
    2014
    Co-Authors: Michiganense Subsp Sepedonicum, Jin Cai, Shulian Xie, Jia Feng, Feipeng Wang
    Abstract:

    Bacterial Ring Rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of Bacterial Ring Rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80uC, an extraction time of 12 h, and a solid to liquid ratio of 1:25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P,0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca2+ concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit pRotein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum

  • pRotective effect of polygonum orientale l extracts against clavibater michiganense subsp sepedonicum the causal agent of Bacterial Ring Rot of potato
    PLOS ONE, 2013
    Co-Authors: Jin Cai, Shulian Xie, Jia Feng, Feipeng Wang
    Abstract:

    The Polygonum orientale L. extracts were investigated for antiBacterial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causal agent of a serious disease called Bacterial Ring Rot of potato. The results showed that the leaf extracts of P. orientale had significantly (p<0.05) greater antiBacterial activity against C. michiganense subsp. sepedonicum than root, stem, flower extracts in vitro. According to the results of single factor experiments and L273(13) orthogonal experiments, optimum extraction conditions were A1B3C1, extraction time 6 h, temperature 80°C, solid to liquid ratio 1∶10 (g:mL). The highest (p<0.05) antiBacterial activity was observed when pH was 5, excluding the effect of control. The extracts were stable under ultraviolet (UV). In vivo analysis revealed that 50 mg/mL of P. orientale leaf extracts was effective in controlling decay. Under field conditions, 50 mg/mL of P. orientale leaf extracts also improved growth parameters (whole plant length, shoot length, root length, plant fresh weight, shoot fresh weight, root fresh weight, dry weight, and number of leaves), in the 2010 and 2011 two growing seasons. Further solvent partition assays showed that the most active compounds were in the petroleum ether fractionation. Transmission electron microscopy (TEM) showed drastic ultrastructural changes caused by petroleum ether fractionation, including Bacterial deformation, electron-dense particles, formation of vacuoles and lack of cytoplasmic materials. These results indicated that P. orientale extracts have strong antiBacterial activity against C. michiganense subsp. sepedonicum and a promising effect in control of Bacterial Ring Rot of potato disease.