The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Masanori Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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The immune response of rainbow trout (Oncorhynchus mykiss) injected with five Renibacterium salmoninarum Bacterins
Aquaculture, 1993Co-Authors: Masahiro Sakai, Shizuo Atsuta, Masanori KobayashiAbstract:Abstract Five Bacterins consisting of formalin-killed, heat-killed, pH-lysed, Streptococcus Bacterin (formalin-killed cells)-mixed, or ultraviolet (UV)-killed cells of Renibacterium salmoninarum were tested for the immune responses elicited in rainbow trout (Oncorhynchus mykiss). Low levels of agglutinating antibody titres were formed in vaccinated fish and in some fish these were undetectable. Phagocytosis was significantly increased in vaccinated fish, except with the Streptococcus-mixed Bacterin, and the chemiluminescent responses of leucocytes were stimulated only with the pH-lysed and UV-killed Bacterins. However, no significant protection was observed in the fish when they were experimentally challenged.
Peter Valentin-weigand - One of the best experts on this subject based on the ideXlab platform.
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Immunogenicity of an Autogenous Streptococcus suis Bacterin in Preparturient Sows and Their Piglets in Relation to Protection after Weaning
Clinical and vaccine immunology : CVI, 2010Co-Authors: Christoph Georg Baums, Christian Brüggemann, Christoph Kock, Andreas Beineke, Karl-heinz Waldmann, Peter Valentin-weigandAbstract:Streptococcus suis is an important porcine pathogen causing meningitis and other invasive diseases in piglets of different ages. Application of S. suis serotype 2 Bacterins to specific-pathogen-free (SPF) weaning piglets has been demonstrated to protect against the homologous serotype. However, autogenous S. suis Bacterins are also applied to sows and suckling piglets in the field. Therefore, comparative evaluation of different Bacterin immunization regimes, including sow vaccination, was performed in this study. The main objectives were to determine the immunogenicity of an S. suis Bacterin in sows prepartum and its influence on active immunization of piglets. Experimental infection of 6- and 8-week-old weaning piglets was performed to elucidate protective efficacies. Humoral immune responses were investigated by an enzyme-linked immunosorbent assay (ELISA) measuring muramidase-released protein (MRP)-specific IgG titers and by opsonophagocytosis assays. Bacterin application elicited high MRP-specific IgG titers in the serum and colostrum of sows, as well as opsonizing antibodies. Piglets from vaccinated sows had significantly higher MRP-specific titers than respective piglets from nonvaccinated sows until 6 weeks postpartum. Vaccination of suckling piglets did not result in high MRP-specific titers nor in induction of opsonizing antibodies. Furthermore, neither vaccination of suckling nor of weaning piglets from immunized sows was associated with a prominent active immune response and protection at 8 weeks postpartum. However, protection was observed in respective 6-week-old weaning piglets, most likely because of protective maternal immunity. In conclusion, this study provides the first results suggesting protective passive maternal immunity for S. suis serotype 2 after Bacterin vaccination of sows and a strong inhibitory effect on active immunization of suckling and weaning piglets, leading to highly susceptible growers.
Masahiro Sakai - One of the best experts on this subject based on the ideXlab platform.
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The immune response of rainbow trout (Oncorhynchus mykiss) injected with five Renibacterium salmoninarum Bacterins
Aquaculture, 1993Co-Authors: Masahiro Sakai, Shizuo Atsuta, Masanori KobayashiAbstract:Abstract Five Bacterins consisting of formalin-killed, heat-killed, pH-lysed, Streptococcus Bacterin (formalin-killed cells)-mixed, or ultraviolet (UV)-killed cells of Renibacterium salmoninarum were tested for the immune responses elicited in rainbow trout (Oncorhynchus mykiss). Low levels of agglutinating antibody titres were formed in vaccinated fish and in some fish these were undetectable. Phagocytosis was significantly increased in vaccinated fish, except with the Streptococcus-mixed Bacterin, and the chemiluminescent responses of leucocytes were stimulated only with the pH-lysed and UV-killed Bacterins. However, no significant protection was observed in the fish when they were experimentally challenged.
Kurt Buchmann - One of the best experts on this subject based on the ideXlab platform.
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positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model
Journal of Fish Diseases, 2017Co-Authors: Moonika Haahr Marana, Jakob Skov, Jiwan Kumar Chettri, B Krossoy, Inger Dalsgaard, Per W Kania, Kurt BuchmannAbstract:Rainbow trout, Oncorhynchus mykiss (Walbaum), are able to raise a protective immune response against Aeromonas salmonicida subsp. salmonicida (AS) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under Danish mariculture conditions. We elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. Rainbow trout juveniles were vaccinated by intraperitoneal (i.p.) injection with a Bacterin of Aeromonas salmonicida subsp. salmonicida strain 090710-1/23 in combination with Vibrio anguillarum serotypes O1 and O2a supplemented with an oil adjuvant. Three concentrations of AS antigens were applied. Fish were subsequently challenged with the homologous bacterial strain administered by perforation of the tail fin epidermis and 60-s contact with live A. salmonicida bacteria. The infection method proved to be efficient and could differentiate efficacies of different vaccines. It was shown that protection and antibody production in exposed fish were positively correlated to the AS antigen concentration in the vaccine.
Christoph Georg Baums - One of the best experts on this subject based on the ideXlab platform.
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Immunogenicity of an Autogenous Streptococcus suis Bacterin in Preparturient Sows and Their Piglets in Relation to Protection after Weaning
Clinical and vaccine immunology : CVI, 2010Co-Authors: Christoph Georg Baums, Christian Brüggemann, Christoph Kock, Andreas Beineke, Karl-heinz Waldmann, Peter Valentin-weigandAbstract:Streptococcus suis is an important porcine pathogen causing meningitis and other invasive diseases in piglets of different ages. Application of S. suis serotype 2 Bacterins to specific-pathogen-free (SPF) weaning piglets has been demonstrated to protect against the homologous serotype. However, autogenous S. suis Bacterins are also applied to sows and suckling piglets in the field. Therefore, comparative evaluation of different Bacterin immunization regimes, including sow vaccination, was performed in this study. The main objectives were to determine the immunogenicity of an S. suis Bacterin in sows prepartum and its influence on active immunization of piglets. Experimental infection of 6- and 8-week-old weaning piglets was performed to elucidate protective efficacies. Humoral immune responses were investigated by an enzyme-linked immunosorbent assay (ELISA) measuring muramidase-released protein (MRP)-specific IgG titers and by opsonophagocytosis assays. Bacterin application elicited high MRP-specific IgG titers in the serum and colostrum of sows, as well as opsonizing antibodies. Piglets from vaccinated sows had significantly higher MRP-specific titers than respective piglets from nonvaccinated sows until 6 weeks postpartum. Vaccination of suckling piglets did not result in high MRP-specific titers nor in induction of opsonizing antibodies. Furthermore, neither vaccination of suckling nor of weaning piglets from immunized sows was associated with a prominent active immune response and protection at 8 weeks postpartum. However, protection was observed in respective 6-week-old weaning piglets, most likely because of protective maternal immunity. In conclusion, this study provides the first results suggesting protective passive maternal immunity for S. suis serotype 2 after Bacterin vaccination of sows and a strong inhibitory effect on active immunization of suckling and weaning piglets, leading to highly susceptible growers.
