Bacteriophage Typing

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Veronika Zajícová - One of the best experts on this subject based on the ideXlab platform.

  • A new Bacteriophage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, Igor Rychlik, J. Pillich, M. Kolářová, J. Seménka, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set for Proteus were examined in 507 Proteus mirabilis and 29 P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in the Myoviridae and Podoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation of Proteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • a new Bacteriophage Typing scheme for proteus mirabilis and proteus vulgaris strains 3 analysis of lytic properties
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, J. Pillich, M. Kolářová, J. Seménka, Ivan Rychlik, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set forProteus were examined in 507Proteus mirabilis and 29P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in theMyoviridae andPodoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation ofProteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • A new phage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1994
    Co-Authors: G. Sekaninová, Veronika Zajícová, M Hofer, M. Kolařík, Igor Rychlik, J. Pillich, D. Kubíčková
    Abstract:

    A new Bacteriophage Typing set, composed of 22 phages, was established as a tool for differentiation of Proteus strains. All the phages were tailed and included 4 morphological types (A1, A2, B1 and C1). They were classified into the families Myoviridae, Siphoviridae and Podoviridae . From the set, 19 phages had double-stranded DNA and 3 were single-stranded DNA phages.

G. Sekaninová - One of the best experts on this subject based on the ideXlab platform.

  • A new Bacteriophage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, Igor Rychlik, J. Pillich, M. Kolářová, J. Seménka, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set for Proteus were examined in 507 Proteus mirabilis and 29 P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in the Myoviridae and Podoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation of Proteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • a new Bacteriophage Typing scheme for proteus mirabilis and proteus vulgaris strains 3 analysis of lytic properties
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, J. Pillich, M. Kolářová, J. Seménka, Ivan Rychlik, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set forProteus were examined in 507Proteus mirabilis and 29P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in theMyoviridae andPodoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation ofProteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • A new phage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1994
    Co-Authors: G. Sekaninová, Veronika Zajícová, M Hofer, M. Kolařík, Igor Rychlik, J. Pillich, D. Kubíčková
    Abstract:

    A new Bacteriophage Typing set, composed of 22 phages, was established as a tool for differentiation of Proteus strains. All the phages were tailed and included 4 morphological types (A1, A2, B1 and C1). They were classified into the families Myoviridae, Siphoviridae and Podoviridae . From the set, 19 phages had double-stranded DNA and 3 were single-stranded DNA phages.

Ingrid Heitmann - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of Molecular Epidemiology of Chilean Salmonella enterica Serotype Enteritidis Isolates by Pulsed-Field Gel Electrophoresis and Bacteriophage Typing
    Journal of clinical microbiology, 2003
    Co-Authors: Jorge Fernández, Alberto Fica, Germán Ebensperger, Hector Calfullan, Soledad Prat, Alda Fernández, Marcela Alexandre, Ingrid Heitmann
    Abstract:

    Human Salmonella enterica serotype Enteritidis infections emerged in Chile in 1994. S. enterica serotype Enteritidis phage type 1 isolates predominated in the north, and phage type 4 isolates predominated in the central and southern regions. A study was planned to characterize this epidemic using the best discriminatory Typing technique. Research involved 441 S. enterica serotype Enteritidis isolates, including clinical preepidemic samples (n = 74; 1975 to 1993) and epidemic (n = 199), food (n = 72), poultry (n = 57), and some Latin American (n = 39) isolates. The best method was selected based on a sample of preepidemic isolates, analyzing the discriminatory power (DP) obtained by phage Typing and randomly amplified polymorphic DNA and pulsed-field gel electophoresis (PFGE) analysis. The highest DP was associated with BlnI PFGE-Bacteriophage Typing analysis (0.993). A total of 38 BlnI patterns (B patterns) were identified before the epidemic period, 19 since 1994, and only 4 in both periods. Two major clusters were identified by phylogenetic analysis, and the predominant B patterns clustered in the same branch. Combined analysis revealed that specific B pattern-phage type combinations (subtypes) disappeared before 1994, that different genotypes associated with S. enterica serotype Enteritidis phage type 4 had been observed since 1988, and that strain diversity increased before the expansion of S. enterica serotype Enteritidis in 1994. Predominant subtype B3-phage type 4 was associated with the central and southern regions, and subtype B38-phage type 1 was associated with the north (P < 0.0001). Food and poultry isolates matched the predominant S. enterica serotype Enteritidis subtypes, but isolates identified in neighboring countries (Peru and Bolivia) did not match S. enterica serotype Enteritidis subtypes identified in the north of Chile. The results of this work demonstrate that genetic diversity, replacement, and expansion of specific S. enterica serotype Enteritidis subtypes were associated with epidemic changes.

J. Pillich - One of the best experts on this subject based on the ideXlab platform.

  • A new Bacteriophage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, Igor Rychlik, J. Pillich, M. Kolářová, J. Seménka, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set for Proteus were examined in 507 Proteus mirabilis and 29 P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in the Myoviridae and Podoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation of Proteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • a new Bacteriophage Typing scheme for proteus mirabilis and proteus vulgaris strains 3 analysis of lytic properties
    Folia Microbiologica, 1998
    Co-Authors: G. Sekaninová, J. Pillich, M. Kolářová, J. Seménka, Ivan Rychlik, Veronika Zajícová
    Abstract:

    The lytic properties of 21 Bacteriophages constituting a new Typing set forProteus were examined in 507Proteus mirabilis and 29P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in theMyoviridae andPodoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the Bacteriophages were not active enough to facilitate the differentiation ofProteus strains The size of the final Typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

  • A new phage Typing scheme forProteus mirabilis andProteus vulgaris strains
    Folia Microbiologica, 1994
    Co-Authors: G. Sekaninová, Veronika Zajícová, M Hofer, M. Kolařík, Igor Rychlik, J. Pillich, D. Kubíčková
    Abstract:

    A new Bacteriophage Typing set, composed of 22 phages, was established as a tool for differentiation of Proteus strains. All the phages were tailed and included 4 morphological types (A1, A2, B1 and C1). They were classified into the families Myoviridae, Siphoviridae and Podoviridae . From the set, 19 phages had double-stranded DNA and 3 were single-stranded DNA phages.

B. A. Oppenheim - One of the best experts on this subject based on the ideXlab platform.

  • SubTyping of methicillin-resistant Staphylococcus aureus isolates from the North-West of England: a comparison of standardised pulsed-field gel electrophoresis with Bacteriophage Typing including an inter-laboratory reproducibility study.
    Journal of medical microbiology, 1999
    Co-Authors: Jon Walker, Ray Borrow, Richard V. Goering, S. Egerton, Andrew J. Fox, B. A. Oppenheim
    Abstract:

    Summary Bacteriophage Typing is currently the recognised methodology for the Typing of methicillin-resistant Staphylococcus aureus (MRSA) in the UK. Bacteriophage Typing is less discriminatory and does not type all isolates compared with some molecular methods for Typing MRSA. Chromosomal genoTyping by pulsed-field gel electrophoresis (PFGE) is increasingly recognised as an improved method for Typing MRSA, providing increased discrimination and typability. In this study the results of a comparison of Bacteriophage Typing and PFGE Typing and subTyping are presented for a large collection of isolates from the North-West of England. Isolates belonging to the most frequently isolated epidemic methicillin-resistant Staphylococcus aureus (EMRSA) Bacteriophage types 15 and 16 were typed by PFGE with further discrimination of common PFGE types possible into a number of subtypes. These results for a large collection of isolates demonstrate the improved Typing of MRSA with PFGE. The widespread acceptance of PFGE for Typing MRSA isolates has been hampered by the lack of standardised methodologies. Recently, a standardised PFGE strain Typing system, known as the GenePath system has become available. The results of an inter-laboratory comparison of PFGE Typing for a collection of isolates demonstrated good reproducibility with this system.