The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Yang Guan-e - One of the best experts on this subject based on the ideXlab platform.
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Content Determination of Total Triterpenes Compound and Oleanolic Acid in Viscum coloratum and Its Photosynthetic Bacterium Transformation Products
China Pharmacy, 2011Co-Authors: Yang Guan-eAbstract:OBJECTIVE:To establish the methods for the content determination of total triterpenes compound and oleanolic acid in Viscum coloratum and its photosynthetic Bacterium Transformation products,and to compare the contents of each sample.METHODS:With oleanolic acid as control,total triterpenes compound was measured by UV spectrophotometry with 5% vanillin-glacial acetic acid solution and perchloric acid as color-developing agent at detection wavelength of 548 nm.The content of oleanolic acid was determined by HPLC.The determination was performed on Diamonsil C18(200 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.18% phosphoric acid water(86:14) with the detection wavelength at 210 nm and the column temperature at 25 ℃.RESULTS:The linear range of total triterpenes compound was 2～12 μg·mL-1(r=0.995 3),and the linear range of oleanolic acid was 0.41～4.1 μg·mL-1(r=0.999 1).75% ethanol extracts of V.coloratum was transformed by Rhodobacter sphaeroides and Rhodopseudomonas palustris respectively.The contents of the total triterpenes increased by 36.0% and 14.7%,and the contents of oleanolic acid increased by 880.0% and 260.0%,respectively.CONCLUSION:The contents of total triterpenes and oleanolic acid in 75% ethanol extracts of mistletoe could be increased by the Transformation of two kinds of photosynthetic Bacterium.It may lead to the production of some enzymes.The experiments lay a foundation for the research of chemical constituents and Transformation mechanism of V.coloratum.
Guangmin Xia - One of the best experts on this subject based on the ideXlab platform.
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TI PLASMID CONSTRUCTION OF BYDV RESISTANT GENE
Journal of Shandong University, 2000Co-Authors: Guangmin XiaAbstract:A new AgroBacterium tumefaciens/Ti plasmid system is supplied with BYDV (Barley yellow dwarf virus) resistant gene for wheat Transformation. By using gene clone methods, such as restriction endonuclease digest, ligation, Bacterium Transformation, and so on, plasmid pPPI12 with BYDV gene was inserted into T-DNA region of Ti plasmid pGA482.
József Szeberényi - One of the best experts on this subject based on the ideXlab platform.
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Test: Construction of genomic libraries
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 2006Co-Authors: József SzeberényiAbstract:Terms to be familiar with before you start to solve the test: genomic library, gel filtration, restriction endonuclease, plasmid, sticky ends, blunt ends, ligation, recombinant DNA, Bacterium Transformation, denaturation and renaturation of DNA, satellite DNA, telomere, centromere, unique and repetitive sequences.
Liang Ai-hua - One of the best experts on this subject based on the ideXlab platform.
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Insect Resistance of CpTI Gene-improved Chinese Cabbage
2006Co-Authors: Zhao Jun-liang, Liang Ai-huaAbstract:Cotyledons were cut from aseptic seedlings of 8 Chinese cabbage materials near their growth points and pre-cultured on the medium of MS+1 mg/L 2,4-D for 48 hours.Then they are infected by A.tumefaciens LBA4404 whose OD_(600) ranged within 0.3~0.4 for three minutes by employing plasmid pBINΩSCK carrying CpTI gene and then cultured on the medium of MS+2 mg/L BA+0.5 mg/L NAA+5 mg/L silver nitrate+2 % sucrose+8 g/L agar for 48 hours.After this,they were transferred to the screening medium containing kanamycin and cefetaxime and produced a lot of transformants four weeks later.The transformants were proved by molecular hybridization that CpTI gene had been integrated in the genome of Chinese cabbage and indoor and field experiments also proved that CpTI gene had bestowed Chinese cabbage with a strong insect resistance.In addition,this study optimized various factors concerned with the agro Bacterium Transformation efficiency and plant regeneration.
Zhao Jun-liang - One of the best experts on this subject based on the ideXlab platform.
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Insect Resistance of CpTI Gene-improved Chinese Cabbage
2006Co-Authors: Zhao Jun-liang, Liang Ai-huaAbstract:Cotyledons were cut from aseptic seedlings of 8 Chinese cabbage materials near their growth points and pre-cultured on the medium of MS+1 mg/L 2,4-D for 48 hours.Then they are infected by A.tumefaciens LBA4404 whose OD_(600) ranged within 0.3~0.4 for three minutes by employing plasmid pBINΩSCK carrying CpTI gene and then cultured on the medium of MS+2 mg/L BA+0.5 mg/L NAA+5 mg/L silver nitrate+2 % sucrose+8 g/L agar for 48 hours.After this,they were transferred to the screening medium containing kanamycin and cefetaxime and produced a lot of transformants four weeks later.The transformants were proved by molecular hybridization that CpTI gene had been integrated in the genome of Chinese cabbage and indoor and field experiments also proved that CpTI gene had bestowed Chinese cabbage with a strong insect resistance.In addition,this study optimized various factors concerned with the agro Bacterium Transformation efficiency and plant regeneration.
