The Experts below are selected from a list of 6459 Experts worldwide ranked by ideXlab platform
Jamie Voyles - One of the best experts on this subject based on the ideXlab platform.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Non-lethal isolation of the fungal pathogen Batrachochytrium dendrobatidis (Bd) from amphibians.
Diseases of Aquatic Organisms, 2018Co-Authors: Kimberly J. Cook, Jamie Voyles, Heather V. Kenny, Karen L. Pope, Jonah Piovia-scottAbstract:The ability to isolate and purify pathogens is important for the study of infectious disease. A protocol for isolating Batrachochytrium dendrobatidis (Bd), a lethal pathogen of amphibians, has been available for over a decade, but the method relies on sacrificing infected animals. We validated a non-lethal protocol for Bd isolation that uses biopsy punches from toe webbing to collect skin samples from live amphibians in remote field locations. We successfully isolated Bd from the Cascades frog Rana cascadae and found a positive association between Bd infection and probability of Bd growth in culture. Recapture rates of sampled animals suggest that our isolation protocol did not affect frog survival. The ability to collect isolates from live animals will facilitate investigations of the biology of Bd and enhance amphibian conservation efforts.
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Insights From Genomics Into Spatial and Temporal Variation in Batrachochytrium dendrobatidis
Progress in Molecular Biology and Translational Science, 2016Co-Authors: Allison Q Byrne, Gabriela Rios-sotelo, Jamie Voyles, Erica Bree RosenblumAbstract:Advances in genetics and genomics have provided new tools for the study of emerging infectious diseases. Researchers can now move quickly from simple hypotheses to complex explanations for pathogen origin, spread, and mechanisms of virulence. Here we focus on the application of genomics to understanding the biology of the fungal pathogen Batrachochytrium dendrobatidis (Bd), a novel and deadly pathogen of amphibians. We provide a brief history of the system, then focus on key insights into Bd variation garnered from genomics approaches, and finally, highlight new frontiers for future discoveries. Genomic tools have revealed unexpected complexity and variation in the Bd system suggesting that the history and biology of emerging pathogens may not be as simple as they initially seem.
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interactions between Batrachochytrium dendrobatidis and its amphibian hosts a review of pathogenesis and immunity
Microbes and Infection, 2011Co-Authors: Jamie Voyles, Erica Bree Rosenblum, Lee BergerAbstract:The fungus Batrachochytrium dendrobatidis (Bd) causes a lethal skin disease of amphibians, chytridiomycosis, which has caused catastrophic amphibian die-offs around the world. This review provides a summary of host characteristics, pathogen characteristics and host-pathogen responses to infection that are important for understanding disease development.
Gabriela Rios-sotelo - One of the best experts on this subject based on the ideXlab platform.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Insights From Genomics Into Spatial and Temporal Variation in Batrachochytrium dendrobatidis
Progress in Molecular Biology and Translational Science, 2016Co-Authors: Allison Q Byrne, Gabriela Rios-sotelo, Jamie Voyles, Erica Bree RosenblumAbstract:Advances in genetics and genomics have provided new tools for the study of emerging infectious diseases. Researchers can now move quickly from simple hypotheses to complex explanations for pathogen origin, spread, and mechanisms of virulence. Here we focus on the application of genomics to understanding the biology of the fungal pathogen Batrachochytrium dendrobatidis (Bd), a novel and deadly pathogen of amphibians. We provide a brief history of the system, then focus on key insights into Bd variation garnered from genomics approaches, and finally, highlight new frontiers for future discoveries. Genomic tools have revealed unexpected complexity and variation in the Bd system suggesting that the history and biology of emerging pathogens may not be as simple as they initially seem.
Alexa L. Lindauer - One of the best experts on this subject based on the ideXlab platform.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
Alexandra A Roberts - One of the best experts on this subject based on the ideXlab platform.
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a rapid and inexpensive viability assay for zoospores and zoosporangia of Batrachochytrium dendrobatidis
Journal of Microbiological Methods, 2019Co-Authors: Rebecca J Webb, Lee F Skerratt, Lee Berger, Alexandra A RobertsAbstract:Abstract The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.
Ciara Sheets - One of the best experts on this subject based on the ideXlab platform.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
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Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability.
Ecohealth, 2019Co-Authors: Alexa L. Lindauer, Gabriela Rios-sotelo, Ciara Sheets, Jamie VoylesAbstract:The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens’ growth and reproduction to improve our understanding of chytridiomycosis.
