The Experts below are selected from a list of 4740 Experts worldwide ranked by ideXlab platform
Yasuyuki Hashidoko - One of the best experts on this subject based on the ideXlab platform.
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO − ) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO − , and the absorbance decreases were suppressed in the presence of glutathione, a ONOO − scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO − monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO − concentration. Betanin inhibited the ONOO − (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05–1.0 mM) also inhibited ONOO − (0.5 mM)-dependent DNA strand clea...
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO(-)) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO(-), and the absorbance decreases were suppressed in the presence of glutathione, a ONOO(-) scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO(-) monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO(-) concentration. Betanin inhibited the ONOO(-) (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC(50) value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 mM) also inhibited ONOO(-) (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses.
Gary D. Stoner - One of the best experts on this subject based on the ideXlab platform.
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drinking water with red Beetroot food color antagonizes esophageal carcinogenesis in n nitrosomethylbenzylamine treated rats
Journal of Medicinal Food, 2010Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Claudio M Rocha, Bethany Larue, Cassandra Henry, Colleen Mcintyre, Ken M Riedl, Gary D. StonerAbstract:This study was undertaken to determine if the oral consumption of red Beetroot food color would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 microg/mL commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (P < .001) in animals that received the food color compared to controls. The treatment also resulted in reduced rates of cell proliferation in both precancerous esophageal lesions and in papillomas of NMBA-treated rats, as measured by immunohistochemical staining of Ki-67 in esophageal tissue specimens. The effects of Beetroot food color on angiogenesis (microvessel density by CD34 immunostaining), inflammation (by CD45 immunostaining), and apoptosis (by terminal deoxynucleotidyl transferase dUTP nick end-labeling staining) in esophageal tissue specimens were also determined. Compared to rats treated with NMBA only, the levels of angiogenesis and inflammation in the Beetroot color-consuming animals were reduced, and the apoptotic rate was increased. Thus, the mechanism(s) of chemoprevention by the active constituents of red Beetroot color include reducing cell proliferation, angiogenesis, and inflammation and stimulating apoptosis. Importantly, consumption of the dye in the drinking water for a period of 35 weeks did not appear to induce any overt toxicity. Based on the fact that red Beetroot color contains betanins, which have strong antioxidant activity, it is postulated that these effects are mediated through inhibition of oxygen radical-induced signal transduction. However, the sum of constituents of E162 has not been determined, and other components with other mechanisms may also be involved in antagonizing cancer development.
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abstract a47 drinking water with red Beetroot food dye antagonizes esophageal carcinogenesis in nmba treated rats
Cancer Prevention Research, 2008Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Gary D. StonerAbstract:A47 This study was undertaken to determine if the oral consumption of red Beetroot dye would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 µg/ml of commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (p Citation Information: Cancer Prev Res 2008;1(7 Suppl):A47.
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abstract a47 drinking water with red Beetroot food dye antagonizes esophageal carcinogenesis in nmba treated rats
Cancer Prevention Research, 2008Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Gary D. StonerAbstract:A47 This study was undertaken to determine if the oral consumption of red Beetroot dye would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 µg/ml of commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (p Citation Information: Cancer Prev Res 2008;1(7 Suppl):A47.
Yasuko Sakihama - One of the best experts on this subject based on the ideXlab platform.
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO − ) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO − , and the absorbance decreases were suppressed in the presence of glutathione, a ONOO − scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO − monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO − concentration. Betanin inhibited the ONOO − (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05–1.0 mM) also inhibited ONOO − (0.5 mM)-dependent DNA strand clea...
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO(-)) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO(-), and the absorbance decreases were suppressed in the presence of glutathione, a ONOO(-) scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO(-) monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO(-) concentration. Betanin inhibited the ONOO(-) (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC(50) value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 mM) also inhibited ONOO(-) (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses.
John F Lechner - One of the best experts on this subject based on the ideXlab platform.
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drinking water with red Beetroot food color antagonizes esophageal carcinogenesis in n nitrosomethylbenzylamine treated rats
Journal of Medicinal Food, 2010Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Claudio M Rocha, Bethany Larue, Cassandra Henry, Colleen Mcintyre, Ken M Riedl, Gary D. StonerAbstract:This study was undertaken to determine if the oral consumption of red Beetroot food color would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 microg/mL commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (P < .001) in animals that received the food color compared to controls. The treatment also resulted in reduced rates of cell proliferation in both precancerous esophageal lesions and in papillomas of NMBA-treated rats, as measured by immunohistochemical staining of Ki-67 in esophageal tissue specimens. The effects of Beetroot food color on angiogenesis (microvessel density by CD34 immunostaining), inflammation (by CD45 immunostaining), and apoptosis (by terminal deoxynucleotidyl transferase dUTP nick end-labeling staining) in esophageal tissue specimens were also determined. Compared to rats treated with NMBA only, the levels of angiogenesis and inflammation in the Beetroot color-consuming animals were reduced, and the apoptotic rate was increased. Thus, the mechanism(s) of chemoprevention by the active constituents of red Beetroot color include reducing cell proliferation, angiogenesis, and inflammation and stimulating apoptosis. Importantly, consumption of the dye in the drinking water for a period of 35 weeks did not appear to induce any overt toxicity. Based on the fact that red Beetroot color contains betanins, which have strong antioxidant activity, it is postulated that these effects are mediated through inhibition of oxygen radical-induced signal transduction. However, the sum of constituents of E162 has not been determined, and other components with other mechanisms may also be involved in antagonizing cancer development.
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abstract a47 drinking water with red Beetroot food dye antagonizes esophageal carcinogenesis in nmba treated rats
Cancer Prevention Research, 2008Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Gary D. StonerAbstract:A47 This study was undertaken to determine if the oral consumption of red Beetroot dye would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 µg/ml of commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (p Citation Information: Cancer Prev Res 2008;1(7 Suppl):A47.
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abstract a47 drinking water with red Beetroot food dye antagonizes esophageal carcinogenesis in nmba treated rats
Cancer Prevention Research, 2008Co-Authors: John F Lechner, Lishu Wang, Steven J Schwartz, Gary D. StonerAbstract:A47 This study was undertaken to determine if the oral consumption of red Beetroot dye would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78 µg/ml of commercial red Beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (p Citation Information: Cancer Prev Res 2008;1(7 Suppl):A47.
Makiko Maeda - One of the best experts on this subject based on the ideXlab platform.
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO − ) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO − , and the absorbance decreases were suppressed in the presence of glutathione, a ONOO − scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO − monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO − concentration. Betanin inhibited the ONOO − (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05–1.0 mM) also inhibited ONOO − (0.5 mM)-dependent DNA strand clea...
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Beetroot betalain inhibits peroxynitrite mediated tyrosine nitration and dna strand cleavage
Free Radical Research, 2012Co-Authors: Yasuko Sakihama, Makiko Maeda, Makoto Hashimoto, Satoshi Tahara, Yasuyuki HashidokoAbstract:Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red Beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO(-)) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO(-), and the absorbance decreases were suppressed in the presence of glutathione, a ONOO(-) scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red Beetroot betacyanins, treated with ONOO(-) monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO(-) concentration. Betanin inhibited the ONOO(-) (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC(50) value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 mM) also inhibited ONOO(-) (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses.