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Gabriela Mazzanti - One of the best experts on this subject based on the ideXlab platform.

  • Inhibition by Beta-Caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes.
    Mutation research, 2010
    Co-Authors: Antonella Di Sotto, Gabriela Mazzanti, Fabio Carbone, Patrizia Hrelia, Francesca Maffei
    Abstract:

    Beta-Caryophyllene is a bi-cyclic sesquiterpene that occurs in essential oils from several plants. A variety of biological activities have been ascribed to this compound. In particular, it seems to possess anti-carcinogenic properties, due to its capability to induce detoxifying enzymes or to enhance, in vitro and in vivo, the natural killer cell-induced cytotoxicity against tumours. Conversely, the knowledge on the DNA-damaging activity of the substance and its modulation is scanty. Therefore, in this study, we aimed at evaluating the capability of Beta-Caryophyllene to protect cultured human lymphocytes from the genotoxic damage induced by ethyl methanesulfonate (EMS) and colcemid (COL) in the micronucleus assay. To investigate the mechanisms of action of this sesquiterpene, the cultures were treated with the compound before (pre-treatment), during (co-treatment) and after (post-treatment) treatment with the mutagens. Up to 100 microg/ml, Beta-Caryophyllene by itself did not produce any cytotoxic and genotoxic effect, as shown by the value of the nuclear division index (NDI) and the frequency of micronuclei (MN). The test compound (0.1-100 microg/ml) significantly reduced the MN frequency induced by EMS in pre- and co-treatment protocols (up to 64.4% and 87% reduction, respectively). In the post-treatment protocol, Beta-Caryophyllene was not effective as an antimutagen. No significant reduction of COL-induced MN frequency was found. The antigenotoxic activity of Beta-Caryophyllene observed here suggests that this compound could act by chemical interaction with the mutagen in the growth medium (co-treatment) or in the cytoplasm of lymphocytes (pre-treatment), so acting as a desmutagen. These data encourage further studies to investigate the mode of action and the potential use of this compound as a chemopreventive agent.

  • Inhibition by β-caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Antonella Di Sotto, Gabriela Mazzanti, Fabio Carbone, Patrizia Hrelia, Francesca Maffei
    Abstract:

    Beta-Caryophyllene is a bi-cyclic sesquiterpene that occurs in essential oils from several plants. A variety of biological activities have been ascribed to this compound. In particular, it seems to possess anti-carcinogenic properties, due to its capability to induce detoxifying enzymes or to enhance, in vitro and in vivo, the natural killer cell-induced cytotoxicity against tumours. Conversely, the knowledge on the DNA-damaging activity of the substance and its modulation is scanty. Therefore, in this study, we aimed at evaluating the capability of Beta-Caryophyllene to protect cultured human lymphocytes from the genotoxic damage induced by ethyl methanesulfonate (EMS) and colcemid (COL) in the micronucleus assay. To investigate the mechanisms of action of this sesquiterpene, the cultures were treated with the compound before (pre-treatment), during (co-treatment) and after (post-treatment) treatment with the mutagens. Up to 100 mu g/ml, Beta-Caryophyllene by itself did not produce any cytotoxic and genotoxic effect, as shown by the value of the nuclear division index (NDI) and the frequency of micronuclei (MN). The test compound (0.1-100 mu g/ml) significantly reduced the MN frequency induced by EMS in pre- and co-treatment protocols (up to 64.4% and 87% reduction, respectively). In the post-treatment protocol, Beta-Caryophyllene was not effective as an antimutagen. No significant reduction of COL-induced MN frequency was found. The antigenotoxic activity of Beta-Caryophyllene observed here suggests that this compound could act by chemical interaction with the mutagen in the growth medium (co-treatment) or in the cytoplasm of lymphocytes (pre-treatment), so acting as a desmutagen. These data encourage further studies to investigate the mode of action and the potential use of this compound as a chemopreventive agent. (C) 2010 Elsevier B.V. All rights reserved

  • Antimutagenic and mutagenic activities of some terpenes in the bacterial reverse mutation assay.
    Mutation research, 2008
    Co-Authors: Antonella Di Sotto, Maria Grazia Evandri, Gabriela Mazzanti
    Abstract:

    The mutagenic and antimutagenic effects of linalool, linalyl acetate and Beta-Caryophyllene were evaluated by the bacterial reverse mutation assay on Salmonella typhimurium TA 98 and TA 100, and on Escherichia coli WP2uvrA strains. Neither linalool nor Beta-Caryophyllene showed mutagenicity, but linalyl acetate induced a statistically significant increase in the number of revertant colonies in WP2uvrA, both with and without S9 mixture. Linalool was devoid of antimutagenic activity against 2-nitrofluorene (2NF), sodium azide (SA), methyl methane sulfonate (MMS) and 2-aminoanthracene (2AA). In contrast, Beta-Caryophyllene showed a strong antimutagenic activity against 2NF: at the maximum concentration tested (6.40mg/plate) the number of 2NF-induced revertant colonies was reduced by 83.9%. Beta-Caryophyllene also showed to counteract the mutagenicity of SA (in TA 100), MMS and 2AA (in WP2uvrA): the effect was weak against SA (inhibition lower than 25%) and moderate against MMS and 2AA (up to 30.5%). The antimutagenic activity of Beta-Caryophyllene observed here suggests further studies to evaluate its possible chemopreventive properties.

  • Antimutagenic and mutagenic activities of some terpenes in the bacterial reverse mutation assay
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2008
    Co-Authors: Antonella Di Sotto, Maria Grazia Evandri, Gabriela Mazzanti
    Abstract:

    The mutagenic and antimutagenic effects of linalool, linalyl acetate and P-caryophyllene were evaluated by the bacterial reverse mutation assay on Salmonella typhimurium TA98 and TA 100,and on Escherichia coli WP2uvrA strains. Neither linalool nor Beta-Caryophyllene showed mutagenicity, but linalyl acetate induced a statistically significant increase in the number of revertant colonies in WP2uvrA, both with and without S9 mixture. Linalool was devoid of antimutagenic activity against 2-nitrofluorene (2NF). sodium azide (SA), methyl methane sulfonate (MMS) and 2-aminoanthracene (2AA). In contrast, Beta-Caryophyllene showed a strong antimutagenic activity against 2NF: at the maximum concentration tested (6.40 mg/plate) the number of 2NF-induced revertant colonies was reduced by 83.9%. Beta-Caryophyllene also showed to counteract the mutagenicity of SA (in TA 100), MMS and 2AA (in WP2uvrA): the effect was weak against SA (inhibition lower than 25%) and moderate against MMS and 2AA (up to 30.5%). The antimutagenic activity of Beta-Caryophyllene observed here suggests further studies to evaluate its possible chemopreventive properties. (C) 2008 Elsevier B.V. All rights reserved

Antonella Di Sotto - One of the best experts on this subject based on the ideXlab platform.

  • Inhibition by Beta-Caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes.
    Mutation research, 2010
    Co-Authors: Antonella Di Sotto, Gabriela Mazzanti, Fabio Carbone, Patrizia Hrelia, Francesca Maffei
    Abstract:

    Beta-Caryophyllene is a bi-cyclic sesquiterpene that occurs in essential oils from several plants. A variety of biological activities have been ascribed to this compound. In particular, it seems to possess anti-carcinogenic properties, due to its capability to induce detoxifying enzymes or to enhance, in vitro and in vivo, the natural killer cell-induced cytotoxicity against tumours. Conversely, the knowledge on the DNA-damaging activity of the substance and its modulation is scanty. Therefore, in this study, we aimed at evaluating the capability of Beta-Caryophyllene to protect cultured human lymphocytes from the genotoxic damage induced by ethyl methanesulfonate (EMS) and colcemid (COL) in the micronucleus assay. To investigate the mechanisms of action of this sesquiterpene, the cultures were treated with the compound before (pre-treatment), during (co-treatment) and after (post-treatment) treatment with the mutagens. Up to 100 microg/ml, Beta-Caryophyllene by itself did not produce any cytotoxic and genotoxic effect, as shown by the value of the nuclear division index (NDI) and the frequency of micronuclei (MN). The test compound (0.1-100 microg/ml) significantly reduced the MN frequency induced by EMS in pre- and co-treatment protocols (up to 64.4% and 87% reduction, respectively). In the post-treatment protocol, Beta-Caryophyllene was not effective as an antimutagen. No significant reduction of COL-induced MN frequency was found. The antigenotoxic activity of Beta-Caryophyllene observed here suggests that this compound could act by chemical interaction with the mutagen in the growth medium (co-treatment) or in the cytoplasm of lymphocytes (pre-treatment), so acting as a desmutagen. These data encourage further studies to investigate the mode of action and the potential use of this compound as a chemopreventive agent.

  • Inhibition by β-caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Antonella Di Sotto, Gabriela Mazzanti, Fabio Carbone, Patrizia Hrelia, Francesca Maffei
    Abstract:

    Beta-Caryophyllene is a bi-cyclic sesquiterpene that occurs in essential oils from several plants. A variety of biological activities have been ascribed to this compound. In particular, it seems to possess anti-carcinogenic properties, due to its capability to induce detoxifying enzymes or to enhance, in vitro and in vivo, the natural killer cell-induced cytotoxicity against tumours. Conversely, the knowledge on the DNA-damaging activity of the substance and its modulation is scanty. Therefore, in this study, we aimed at evaluating the capability of Beta-Caryophyllene to protect cultured human lymphocytes from the genotoxic damage induced by ethyl methanesulfonate (EMS) and colcemid (COL) in the micronucleus assay. To investigate the mechanisms of action of this sesquiterpene, the cultures were treated with the compound before (pre-treatment), during (co-treatment) and after (post-treatment) treatment with the mutagens. Up to 100 mu g/ml, Beta-Caryophyllene by itself did not produce any cytotoxic and genotoxic effect, as shown by the value of the nuclear division index (NDI) and the frequency of micronuclei (MN). The test compound (0.1-100 mu g/ml) significantly reduced the MN frequency induced by EMS in pre- and co-treatment protocols (up to 64.4% and 87% reduction, respectively). In the post-treatment protocol, Beta-Caryophyllene was not effective as an antimutagen. No significant reduction of COL-induced MN frequency was found. The antigenotoxic activity of Beta-Caryophyllene observed here suggests that this compound could act by chemical interaction with the mutagen in the growth medium (co-treatment) or in the cytoplasm of lymphocytes (pre-treatment), so acting as a desmutagen. These data encourage further studies to investigate the mode of action and the potential use of this compound as a chemopreventive agent. (C) 2010 Elsevier B.V. All rights reserved

  • Antimutagenic and mutagenic activities of some terpenes in the bacterial reverse mutation assay.
    Mutation research, 2008
    Co-Authors: Antonella Di Sotto, Maria Grazia Evandri, Gabriela Mazzanti
    Abstract:

    The mutagenic and antimutagenic effects of linalool, linalyl acetate and Beta-Caryophyllene were evaluated by the bacterial reverse mutation assay on Salmonella typhimurium TA 98 and TA 100, and on Escherichia coli WP2uvrA strains. Neither linalool nor Beta-Caryophyllene showed mutagenicity, but linalyl acetate induced a statistically significant increase in the number of revertant colonies in WP2uvrA, both with and without S9 mixture. Linalool was devoid of antimutagenic activity against 2-nitrofluorene (2NF), sodium azide (SA), methyl methane sulfonate (MMS) and 2-aminoanthracene (2AA). In contrast, Beta-Caryophyllene showed a strong antimutagenic activity against 2NF: at the maximum concentration tested (6.40mg/plate) the number of 2NF-induced revertant colonies was reduced by 83.9%. Beta-Caryophyllene also showed to counteract the mutagenicity of SA (in TA 100), MMS and 2AA (in WP2uvrA): the effect was weak against SA (inhibition lower than 25%) and moderate against MMS and 2AA (up to 30.5%). The antimutagenic activity of Beta-Caryophyllene observed here suggests further studies to evaluate its possible chemopreventive properties.

  • Antimutagenic and mutagenic activities of some terpenes in the bacterial reverse mutation assay
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2008
    Co-Authors: Antonella Di Sotto, Maria Grazia Evandri, Gabriela Mazzanti
    Abstract:

    The mutagenic and antimutagenic effects of linalool, linalyl acetate and P-caryophyllene were evaluated by the bacterial reverse mutation assay on Salmonella typhimurium TA98 and TA 100,and on Escherichia coli WP2uvrA strains. Neither linalool nor Beta-Caryophyllene showed mutagenicity, but linalyl acetate induced a statistically significant increase in the number of revertant colonies in WP2uvrA, both with and without S9 mixture. Linalool was devoid of antimutagenic activity against 2-nitrofluorene (2NF). sodium azide (SA), methyl methane sulfonate (MMS) and 2-aminoanthracene (2AA). In contrast, Beta-Caryophyllene showed a strong antimutagenic activity against 2NF: at the maximum concentration tested (6.40 mg/plate) the number of 2NF-induced revertant colonies was reduced by 83.9%. Beta-Caryophyllene also showed to counteract the mutagenicity of SA (in TA 100), MMS and 2AA (in WP2uvrA): the effect was weak against SA (inhibition lower than 25%) and moderate against MMS and 2AA (up to 30.5%). The antimutagenic activity of Beta-Caryophyllene observed here suggests further studies to evaluate its possible chemopreventive properties. (C) 2008 Elsevier B.V. All rights reserved

Leopold Jirovetz - One of the best experts on this subject based on the ideXlab platform.

  • Aroma compound analysis of Piper nigrum and Piper guineense essential oils from Cameroon using solid-phase microextraction-gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry and olfactometry.
    Journal of chromatography. A, 2002
    Co-Authors: Leopold Jirovetz, Martin Benoit Ngassoum, Gerhard Buchbauer, Margit Geissler
    Abstract:

    The investigation of aroma compounds of the essential oils of dried fruits of black pepper (Piper nigrum) and black and white "Ashanti pepper" (Piper guineense) from Cameroon by means of solid-phase microextraction (SPME) was carried out for the first time to identify the odorous target components responsible for the characteristic odor of these valuable spices and food flavoring products. By means of GC-flame ionization detection (FID) and GC-MS (using different polar columns) the main compounds (concentration >3.0%, calculated as area of GC-FID analysis using a non-polar fused-silica open tubular RSL-200 column) of the SPME headspace samples of P. nigrum (black) and P. guineense (black and white) were found to be: P. nigrum (black)--germacrene D (11.01%), limonene (10.26%), beta-pinene (10.02%), alpha-phellandrene (8.56%), Beta-Caryophyllene (7.29%), alpha-pinene (6.40%) and cis-beta-ocimene (3.19%); P. guineense (black)--Beta-Caryophyllene (57.59%), beta-elemene (5.10%), bicyclogermacrene (5.05%) and alpha-humulene (4.86%); and P. guineense (white)--Beta-Caryophyllene (51.75%), cis-beta-ocimene (6.61%), limonene (5.88%), beta-pinene (4.56%), linalool (3.97%) and alpha-humulene (3.29%). The most intense odor impressions of the essential oils of the various dried pepper fruits were given byprofessional perfumers as follows: P nigrum (black)--fine, pleasant black pepper note; P. guineense (black)--black pepper top-note; and P. guineense (white)--pleasant white pepper note. These analytical results for the SPME headspace samples of three different pepper species from Cameroon are in accordance with the olfactoric data of the corresponding essential oils. A GC-sniffing technique was used to correlate the single odor impression of the identified SPME headspace volatiles of the three investigated pepper samples with the following results: themain compounds such as Beta-Caryophyllene, germacrene D, limonene, beta-pinene, alpha-phellandrene and alpha-humulene, as well as minor constituents such as delta-carene, beta-phellandrene, isoborneol, alpha-guaiene, sarisan, elemicin, calamenene, caryophyllene alcohol, isoelemicin, T-muurolol, cubenol and bulnesol, are of greatest importance for the characteristic pepper odor notes of these three Piper samples. Further aroma impressions can be attributed to mono- and sesquiterpenes, hexane, octane and nonane derivatives.

  • Analysis of leaf volatiles of Zanthoxylum gillettii used in folk medicine of Cameroon.
    Planta Medica, 1999
    Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Wilhelm Fleischhacker, Martin Benoit Ngassoum
    Abstract:

    : The essential oil and the corresponding headspace sample of the leaves of ZONTHIOXYLUM GILLETTII (Rutaceae) were analyzed by gas chromatographic-spectroscopic and olfactoric methods. Mainly monoterpenes such as myrcene, TRANS-beta-ocimene, limonene, camphene, alpha- and beta-pinene which are impact compounds were found in the essential oil and headspace sample. Sesquiterpenes such as Beta-Caryophyllene, alpha- and beta-farnesene, beta-bisabolol, gamma-cadinene and nerolidol and alphatic components such as 2-undecanone and dodecanal were also identified and contribute to the typical odor impression as well as to the reported therapeutic properties of this ZONTHOXYLUM species.

Margit Geissler - One of the best experts on this subject based on the ideXlab platform.

  • Aroma compound analysis of Piper nigrum and Piper guineense essential oils from Cameroon using solid-phase microextraction-gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry and olfactometry.
    Journal of chromatography. A, 2002
    Co-Authors: Leopold Jirovetz, Martin Benoit Ngassoum, Gerhard Buchbauer, Margit Geissler
    Abstract:

    The investigation of aroma compounds of the essential oils of dried fruits of black pepper (Piper nigrum) and black and white "Ashanti pepper" (Piper guineense) from Cameroon by means of solid-phase microextraction (SPME) was carried out for the first time to identify the odorous target components responsible for the characteristic odor of these valuable spices and food flavoring products. By means of GC-flame ionization detection (FID) and GC-MS (using different polar columns) the main compounds (concentration >3.0%, calculated as area of GC-FID analysis using a non-polar fused-silica open tubular RSL-200 column) of the SPME headspace samples of P. nigrum (black) and P. guineense (black and white) were found to be: P. nigrum (black)--germacrene D (11.01%), limonene (10.26%), beta-pinene (10.02%), alpha-phellandrene (8.56%), Beta-Caryophyllene (7.29%), alpha-pinene (6.40%) and cis-beta-ocimene (3.19%); P. guineense (black)--Beta-Caryophyllene (57.59%), beta-elemene (5.10%), bicyclogermacrene (5.05%) and alpha-humulene (4.86%); and P. guineense (white)--Beta-Caryophyllene (51.75%), cis-beta-ocimene (6.61%), limonene (5.88%), beta-pinene (4.56%), linalool (3.97%) and alpha-humulene (3.29%). The most intense odor impressions of the essential oils of the various dried pepper fruits were given byprofessional perfumers as follows: P nigrum (black)--fine, pleasant black pepper note; P. guineense (black)--black pepper top-note; and P. guineense (white)--pleasant white pepper note. These analytical results for the SPME headspace samples of three different pepper species from Cameroon are in accordance with the olfactoric data of the corresponding essential oils. A GC-sniffing technique was used to correlate the single odor impression of the identified SPME headspace volatiles of the three investigated pepper samples with the following results: themain compounds such as Beta-Caryophyllene, germacrene D, limonene, beta-pinene, alpha-phellandrene and alpha-humulene, as well as minor constituents such as delta-carene, beta-phellandrene, isoborneol, alpha-guaiene, sarisan, elemicin, calamenene, caryophyllene alcohol, isoelemicin, T-muurolol, cubenol and bulnesol, are of greatest importance for the characteristic pepper odor notes of these three Piper samples. Further aroma impressions can be attributed to mono- and sesquiterpenes, hexane, octane and nonane derivatives.

Martin Benoit Ngassoum - One of the best experts on this subject based on the ideXlab platform.

  • Aroma compound analysis of Piper nigrum and Piper guineense essential oils from Cameroon using solid-phase microextraction-gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry and olfactometry.
    Journal of chromatography. A, 2002
    Co-Authors: Leopold Jirovetz, Martin Benoit Ngassoum, Gerhard Buchbauer, Margit Geissler
    Abstract:

    The investigation of aroma compounds of the essential oils of dried fruits of black pepper (Piper nigrum) and black and white "Ashanti pepper" (Piper guineense) from Cameroon by means of solid-phase microextraction (SPME) was carried out for the first time to identify the odorous target components responsible for the characteristic odor of these valuable spices and food flavoring products. By means of GC-flame ionization detection (FID) and GC-MS (using different polar columns) the main compounds (concentration >3.0%, calculated as area of GC-FID analysis using a non-polar fused-silica open tubular RSL-200 column) of the SPME headspace samples of P. nigrum (black) and P. guineense (black and white) were found to be: P. nigrum (black)--germacrene D (11.01%), limonene (10.26%), beta-pinene (10.02%), alpha-phellandrene (8.56%), Beta-Caryophyllene (7.29%), alpha-pinene (6.40%) and cis-beta-ocimene (3.19%); P. guineense (black)--Beta-Caryophyllene (57.59%), beta-elemene (5.10%), bicyclogermacrene (5.05%) and alpha-humulene (4.86%); and P. guineense (white)--Beta-Caryophyllene (51.75%), cis-beta-ocimene (6.61%), limonene (5.88%), beta-pinene (4.56%), linalool (3.97%) and alpha-humulene (3.29%). The most intense odor impressions of the essential oils of the various dried pepper fruits were given byprofessional perfumers as follows: P nigrum (black)--fine, pleasant black pepper note; P. guineense (black)--black pepper top-note; and P. guineense (white)--pleasant white pepper note. These analytical results for the SPME headspace samples of three different pepper species from Cameroon are in accordance with the olfactoric data of the corresponding essential oils. A GC-sniffing technique was used to correlate the single odor impression of the identified SPME headspace volatiles of the three investigated pepper samples with the following results: themain compounds such as Beta-Caryophyllene, germacrene D, limonene, beta-pinene, alpha-phellandrene and alpha-humulene, as well as minor constituents such as delta-carene, beta-phellandrene, isoborneol, alpha-guaiene, sarisan, elemicin, calamenene, caryophyllene alcohol, isoelemicin, T-muurolol, cubenol and bulnesol, are of greatest importance for the characteristic pepper odor notes of these three Piper samples. Further aroma impressions can be attributed to mono- and sesquiterpenes, hexane, octane and nonane derivatives.

  • Analysis of leaf volatiles of Zanthoxylum gillettii used in folk medicine of Cameroon.
    Planta Medica, 1999
    Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Wilhelm Fleischhacker, Martin Benoit Ngassoum
    Abstract:

    : The essential oil and the corresponding headspace sample of the leaves of ZONTHIOXYLUM GILLETTII (Rutaceae) were analyzed by gas chromatographic-spectroscopic and olfactoric methods. Mainly monoterpenes such as myrcene, TRANS-beta-ocimene, limonene, camphene, alpha- and beta-pinene which are impact compounds were found in the essential oil and headspace sample. Sesquiterpenes such as Beta-Caryophyllene, alpha- and beta-farnesene, beta-bisabolol, gamma-cadinene and nerolidol and alphatic components such as 2-undecanone and dodecanal were also identified and contribute to the typical odor impression as well as to the reported therapeutic properties of this ZONTHOXYLUM species.