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Zubaidah Haji Abdul Rahim - One of the best experts on this subject based on the ideXlab platform.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

Nordin Nur Sazwi - One of the best experts on this subject based on the ideXlab platform.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

Thurairajah Nalina - One of the best experts on this subject based on the ideXlab platform.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

  • antioxidant and cytoprotective activities of piper betle areca catechu uncaria gambir and Betel quid with and without calcium hydroxide
    BMC Complementary and Alternative Medicine, 2013
    Co-Authors: Nordin Nur Sazwi, Thurairajah Nalina, Zubaidah Haji Abdul Rahim
    Abstract:

    Betel quid chewing is a popular habit in Southeast Asia. It is believed that chewing Betel quid could reduce stress, strengthen teeth and maintain oral hygiene. The aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of Betel quid and compared with Betel quid itself (with and without calcium hydroxide). The correlation of their cytoprotective and antioxidant activities with phenolic content was also determined. Five samples (Betel leaf, areca nut, gambir, Betel quid and Betel quid containing calcium hydroxide) were extracted in deionized distilled water for 12 hours at 37°C. Antioxidant activities were evaluated for radical scavenging activity using DPPH assay, ferric reducing activity using FRAP assay and lipid peroxidation inhibition activity using FTC assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu procedure. Phenolic composition was analyzed using LC-MS/MS. Cytoprotective activity towards human gingival fibroblast cells was examined using MTT assay. Among the ingredients of Betel quid, gambir demonstrated the highest antioxidant (DPPH - IC50 = 6.4 ± 0.8 μg/mL, FRAP - 5717.8 ± 537.6 μmol Fe(II)/mg), total phenolic content (TPC - 1142.5 ± 106.8 μg TAE/mg) and cytoprotective (100.1 ± 4.6%) activities. Betel quid when compared with Betel quid containing calcium hydroxide has higher antioxidant (DPPH - IC50 =59.4 ± 4.4 μg/mL, FRAP - 1022.2 ± 235.7 μmol Fe(II)/mg), total phenolic content (TPC - 140.0 ± 22.3 μg TAE/mg), and cytoprotective (113.5 ± 15.9%) activities. However, all of the five samples showed good lipid peroxidation inhibition compared to vitamin E. LC-MS/MS analysis revealed the presence of quinic acid as the major compound of gambir and Betel quid. A positive correlation was observed between TPC and radical scavenging (r = 0.972), reducing power (r = 0.981) and cytoprotective activity (r = 0.682). The Betel quid has higher TPC, and antioxidant and cytoprotective activities than Betel quid with calcium hydroxide. The quinic acid in Betel quid may play an important role in the oral health protection.

Yana Lusi - One of the best experts on this subject based on the ideXlab platform.

  • UJI AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN SIRIH HIJAU (Piper betle L.) TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae SECARA IN VITRO
    2020
    Co-Authors: Yana Lusi
    Abstract:

    One of the medicinal plants in Indonesia and also known by the community. Some of the chemical content of Betel leaf plants, such as saponins, flavonoids, polyphenols, and essential oils (Aiello, 2012). The Betel leaf plant used in modern medicine can be beneficial as an astringent, diuretics, and Antiinflammatory (Kartasapoetra, 1992). This study aims to determine the antibacterial activity of green sirih leaf ethanol (Piper betle L.) of the Shigella dysenteria bacteria. Green Sirih leaf ethanol (Piper betle L.) is obtained using a fractionation method to get secondary metabolite compounds. In the test of the type of compound contained in the green Betel leaf ethanol fraction (Piper betle L.) using thin-layer chromatography (TLC) method. While in this antibacterial test is done using the technique of diffusion discs. The result of the hating zone diameter of the test was at concentrations of 125 mg/ml at 10.69 mm, at concentrations of 250 mg/ml at 11.65 mm, while at a concentration of 500 mg/ml was obtained a barrier zone of 13.57 mm. According to the theory of CLSI (2017) stated that the diameter of the barrier zone obtained is included in the resistant category

Sari, Yulia Purnama - One of the best experts on this subject based on the ideXlab platform.

  • UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSAN DAUN SIRIH HIJAU (Piper betle L.) TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae SECARA IN VITRO
    2020
    Co-Authors: Sari, Yulia Purnama
    Abstract:

    ABSTRACT ANTIBACTERIAL ACTIVITY TEST OF N-HEKSAN LEAF GREEN LEAF FACTION (Piper betle L.) ON THE GROWTH OF Shigella dysentriae BACTERIA IN VITRO Yulia Purnama Sari*, Siti Rofida, Ahmad Shobrun Jamil's Pharmacy Education Program, Faculty of Health Sciences, University of Muhammadiyah Malang * Email: yuliapurnamap@gmail.com One of the medicinal plants in Indonesia and also known by the public. Some of the chemical content of Betel leaf plants such as saponins, flavonoids, polyphenols, and essential oils (Aiello, 2012). Betel leaf plant which is used as a mouth odor remover, mouthwash, eliminate vaginal discharge, digestive damage and various infections (Hoque et al., 2011). This study aims to determine the antibacterial activity of the ethanol fraction of green Betel leaf (Piper betle L.) against Shigella dysentriae. The fraction of n-hexane green Betel leaf (Piper betle L.) was obtained using the fractionation method in order to obtain secondary metabolite compounds. In the Compound Group test contained in the ethanol fraction of green Betel leaf (Piper betle L.) using thin layer chromatography (TLC) method. While the antibacterial testing is done using the disk diffusion method. The results of inhibition zone diameter in this test are at a concentration of 125 mg / ml at 8.30 mm, at a concentration of 250 mg / ml at 9.20 mm, while at a concentration of 500 mg / ml the inhibition zone is obtained at 9.40 mm. According to the theory of CLSI (2017) states that the inhibition zone diameter obtained is included in the category of resistance