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Michael D Wiese - One of the best experts on this subject based on the ideXlab platform.
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factors influencing the quality of myrmecia pilosula jack jumper ant venom for use in in vitro and in vivo diagnosis of allergen sensitization and in allergen immunotherapy
Clinical & Experimental Allergy, 2017Co-Authors: Troy Wanandy, Simon G A Brown, Michael D Wiese, Noel W Davies, He Dwyer, Loyola Mclean, David Nichols, Nuri GuevenAbstract:SummaryBackground Allergen immunotherapy uses pharmaceutical preparations derived from naturally occurring source materials, which contain water-soluble allergenic components responsible for allergic reactions. The success of in vivo and in vitro diagnosis in allergen sensitization and allergen immunotherapy largely depends on the quality, composition and uniformity of allergenic materials used to produce the active ingredients, and the formulation employed to prepare finished products. Objectives We aimed to examine the factors influencing batch-to-batch consistency of Jack Jumper (Myrmecia pilosula) ant venom (JJAV) in the form of Active Pharmaceutical Ingredient (AI) and informed whether factors such as temperature, artificial light and container materials influence the quality of JJAV AIs. We also aimed to establish handling and storage requirements of JJAV AIs to ensure preservation of allergenic activities during usage in the diagnosis of allergen sensitization and in allergen immunotherapy. Methods The quality and consistency of JJAV AIs were analysed using a combination of Bicinchoninic Acid Assay for total protein quantification, HPLC-UV for JJAV allergen peptides quantification, ELISA Inhibition for total allergenic potency, SDS-PAGE, AU-PAGE and Immunoblot for qualitative assessment of JJAV components, and Limulus Amebocyte Lysate Assay for the quantification of endotoxin concentration. API-ZYM and Zymogram Assays were used to probe the presence of enzymatic activities in JJAV. Results Pharmaceutical grade JJAV for allergen immunotherapy has good batch-to-batch consistency. Temporary storage at 4°C and light exposure does not affect the quality of JJAV. Exposure to temperature above 40°C degrades high MW allergens in JJAV. Vials containing JJAV must be stored frozen and in upright position during long-term storage. Conclusions and Clinical relevance We have identified factors which can influence the quality and consistency of JJAV AIs and provided a framework for appropriate handling, transporting and storage of JJAV to be used for the diagnosis of allergen sensitization and in AIT. This article is protected by copyright. All rights reserved.
Troy Wanandy - One of the best experts on this subject based on the ideXlab platform.
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factors influencing the quality of myrmecia pilosula jack jumper ant venom for use in in vitro and in vivo diagnosis of allergen sensitization and in allergen immunotherapy
Clinical & Experimental Allergy, 2017Co-Authors: Troy Wanandy, Simon G A Brown, Michael D Wiese, Noel W Davies, He Dwyer, Loyola Mclean, David Nichols, Nuri GuevenAbstract:SummaryBackground Allergen immunotherapy uses pharmaceutical preparations derived from naturally occurring source materials, which contain water-soluble allergenic components responsible for allergic reactions. The success of in vivo and in vitro diagnosis in allergen sensitization and allergen immunotherapy largely depends on the quality, composition and uniformity of allergenic materials used to produce the active ingredients, and the formulation employed to prepare finished products. Objectives We aimed to examine the factors influencing batch-to-batch consistency of Jack Jumper (Myrmecia pilosula) ant venom (JJAV) in the form of Active Pharmaceutical Ingredient (AI) and informed whether factors such as temperature, artificial light and container materials influence the quality of JJAV AIs. We also aimed to establish handling and storage requirements of JJAV AIs to ensure preservation of allergenic activities during usage in the diagnosis of allergen sensitization and in allergen immunotherapy. Methods The quality and consistency of JJAV AIs were analysed using a combination of Bicinchoninic Acid Assay for total protein quantification, HPLC-UV for JJAV allergen peptides quantification, ELISA Inhibition for total allergenic potency, SDS-PAGE, AU-PAGE and Immunoblot for qualitative assessment of JJAV components, and Limulus Amebocyte Lysate Assay for the quantification of endotoxin concentration. API-ZYM and Zymogram Assays were used to probe the presence of enzymatic activities in JJAV. Results Pharmaceutical grade JJAV for allergen immunotherapy has good batch-to-batch consistency. Temporary storage at 4°C and light exposure does not affect the quality of JJAV. Exposure to temperature above 40°C degrades high MW allergens in JJAV. Vials containing JJAV must be stored frozen and in upright position during long-term storage. Conclusions and Clinical relevance We have identified factors which can influence the quality and consistency of JJAV AIs and provided a framework for appropriate handling, transporting and storage of JJAV to be used for the diagnosis of allergen sensitization and in AIT. This article is protected by copyright. All rights reserved.
He Dwyer - One of the best experts on this subject based on the ideXlab platform.
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factors influencing the quality of myrmecia pilosula jack jumper ant venom for use in in vitro and in vivo diagnosis of allergen sensitization and in allergen immunotherapy
Clinical & Experimental Allergy, 2017Co-Authors: Troy Wanandy, Simon G A Brown, Michael D Wiese, Noel W Davies, He Dwyer, Loyola Mclean, David Nichols, Nuri GuevenAbstract:SummaryBackground Allergen immunotherapy uses pharmaceutical preparations derived from naturally occurring source materials, which contain water-soluble allergenic components responsible for allergic reactions. The success of in vivo and in vitro diagnosis in allergen sensitization and allergen immunotherapy largely depends on the quality, composition and uniformity of allergenic materials used to produce the active ingredients, and the formulation employed to prepare finished products. Objectives We aimed to examine the factors influencing batch-to-batch consistency of Jack Jumper (Myrmecia pilosula) ant venom (JJAV) in the form of Active Pharmaceutical Ingredient (AI) and informed whether factors such as temperature, artificial light and container materials influence the quality of JJAV AIs. We also aimed to establish handling and storage requirements of JJAV AIs to ensure preservation of allergenic activities during usage in the diagnosis of allergen sensitization and in allergen immunotherapy. Methods The quality and consistency of JJAV AIs were analysed using a combination of Bicinchoninic Acid Assay for total protein quantification, HPLC-UV for JJAV allergen peptides quantification, ELISA Inhibition for total allergenic potency, SDS-PAGE, AU-PAGE and Immunoblot for qualitative assessment of JJAV components, and Limulus Amebocyte Lysate Assay for the quantification of endotoxin concentration. API-ZYM and Zymogram Assays were used to probe the presence of enzymatic activities in JJAV. Results Pharmaceutical grade JJAV for allergen immunotherapy has good batch-to-batch consistency. Temporary storage at 4°C and light exposure does not affect the quality of JJAV. Exposure to temperature above 40°C degrades high MW allergens in JJAV. Vials containing JJAV must be stored frozen and in upright position during long-term storage. Conclusions and Clinical relevance We have identified factors which can influence the quality and consistency of JJAV AIs and provided a framework for appropriate handling, transporting and storage of JJAV to be used for the diagnosis of allergen sensitization and in AIT. This article is protected by copyright. All rights reserved.
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Factors influencing the quality of Myrmecia pilosula (Jack Jumper) ant venom for use in in vitro and in vivo diagnosis of allergen sensitization and in allergen immunotherapy
'Wiley', 2017Co-Authors: Wanandy T, Nw Davies, He Dwyer, Mclean L, Nichols D, Gueven N, Brown SgaAbstract:Background: Allergen immunotherapy uses pharmaceutical preparations derived from naturally occurring source materials, which contain water-soluble allergenic components responsible for allergic reactions. The success of in vivo and in vitro diagnoses in allergen sensitization and allergen immunotherapy largely depends on the quality, composition and uniformity of allergenic materials used to produce the active ingredients, and the formulation employed to prepare finished products.Objectives: We aimed to examine the factors influencing batch-to-batch consistency of Jack Jumper (Myrmecia pilosula) ant venom (JJAV) in the form of active pharmaceutical ingredient (AI) and informed whether factors such as temperature, artificial light and container materials influence the quality of JJAV AIs. We also aimed to establish handling and storage requirements of JJAV AIs to ensure preservation of allergenic activities during usage in the diagnosis of allergen sensitization and in allergen immunotherapy.Methods: The quality and consistency of JJAV AIs were analysed using a combination of Bicinchoninic Acid Assay for total protein quantification, HPLC-UV for JJAV allergen peptides quantification, ELISA inhibition for total allergenic potency, SDSPAGE, AU-PAGE and immunoblot for qualitative assessment of JJAV components, and Limulus Amebocyte Lysate Assay for the quantification of endotoxin concentration. API-ZYM and Zymogram Assays were used to probe the presence of enzymatic activities in JJAV.Results: Pharmaceutical-grade JJAV for allergen immunotherapy has good batch-tobatch consistency. Temporary storage at 4°C and light exposure do not affect the quality of JJAV. Exposure to temperature above 40°C degrades high MW allergens in JJAV. Vials containing JJAV must be stored frozen and in upright position during long-term storage.Conclusions and Clinical Relevance: We have identified factors, which can influence the quality and consistency of JJAV AIs, and provided a framework for appropriate handling, transporting and storage of JJAV to be used for the diagnosis of allergen sensitization and in AIT
Kate Howell - One of the best experts on this subject based on the ideXlab platform.
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soluble protein and amino Acid content affects the foam quality of sparkling wine
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Bruna Conde, Sigfredo Fuentes, Eloise Bouchard, Julie A Culbert, Kerry L Wilkinson, Kate HowellAbstract:Proteins and amino Acids are known to influence the foam characteristics of sparkling wines. However, it is unclear to what extent they promote foam formation and/or stability. This study aimed to investigate the effect of protein content and amino Acid composition, measured via the Bicinchoninic Acid Assay and high-performance liquid chromatography, respectively, on the foaming properties of 28 sparkling white wines, made by different production methods. Foam volume and stability were determined using a robotic pourer and computer vision algorithms. Modifications were applied to the protein determination method involving the use of yeast invertase as a standard in order to improve quantification accuracy. The protein content was found to be significantly correlated to parameters representative of foam stability, as were the amino Acids arginine, asparagine, histidine, and tyrosine. Additionally, the production method was found to influence the foam collar height, which favored foaming in Methode Traditionnelle wines over other those made by production methods. Understanding the contributions of key wine constituents to the visual and mouthfeel parameters of sparkling wine will enable more efficient production of high-quality wines.
Noel W Davies - One of the best experts on this subject based on the ideXlab platform.
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factors influencing the quality of myrmecia pilosula jack jumper ant venom for use in in vitro and in vivo diagnosis of allergen sensitization and in allergen immunotherapy
Clinical & Experimental Allergy, 2017Co-Authors: Troy Wanandy, Simon G A Brown, Michael D Wiese, Noel W Davies, He Dwyer, Loyola Mclean, David Nichols, Nuri GuevenAbstract:SummaryBackground Allergen immunotherapy uses pharmaceutical preparations derived from naturally occurring source materials, which contain water-soluble allergenic components responsible for allergic reactions. The success of in vivo and in vitro diagnosis in allergen sensitization and allergen immunotherapy largely depends on the quality, composition and uniformity of allergenic materials used to produce the active ingredients, and the formulation employed to prepare finished products. Objectives We aimed to examine the factors influencing batch-to-batch consistency of Jack Jumper (Myrmecia pilosula) ant venom (JJAV) in the form of Active Pharmaceutical Ingredient (AI) and informed whether factors such as temperature, artificial light and container materials influence the quality of JJAV AIs. We also aimed to establish handling and storage requirements of JJAV AIs to ensure preservation of allergenic activities during usage in the diagnosis of allergen sensitization and in allergen immunotherapy. Methods The quality and consistency of JJAV AIs were analysed using a combination of Bicinchoninic Acid Assay for total protein quantification, HPLC-UV for JJAV allergen peptides quantification, ELISA Inhibition for total allergenic potency, SDS-PAGE, AU-PAGE and Immunoblot for qualitative assessment of JJAV components, and Limulus Amebocyte Lysate Assay for the quantification of endotoxin concentration. API-ZYM and Zymogram Assays were used to probe the presence of enzymatic activities in JJAV. Results Pharmaceutical grade JJAV for allergen immunotherapy has good batch-to-batch consistency. Temporary storage at 4°C and light exposure does not affect the quality of JJAV. Exposure to temperature above 40°C degrades high MW allergens in JJAV. Vials containing JJAV must be stored frozen and in upright position during long-term storage. Conclusions and Clinical relevance We have identified factors which can influence the quality and consistency of JJAV AIs and provided a framework for appropriate handling, transporting and storage of JJAV to be used for the diagnosis of allergen sensitization and in AIT. This article is protected by copyright. All rights reserved.