The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform

Ignacio V. Sandoval - One of the best experts on this subject based on the ideXlab platform.

  • basolateral sorting and transcytosis define the cu regulated translocation of atp7b to the Bile Canaliculus
    Journal of Cell Science, 2016
    Co-Authors: Vasiliki S. Lalioti, Ramón Peiró, Yo Tsuchiya, Angeles Muñoz, Teresa Villalba, Carlos Sánchez, Manuela Perezberlanga, Ignacio V. Sandoval
    Abstract:

    The Cu(+) pump ATP7B plays an irreplaceable role in the elimination of excess Cu(+) by the hepatocyte into the Bile. The trafficking and site of action of ATP7B are subjects of controversy. One current proposal is that an increase in intracellular Cu(+) results in the translocation of ATP7B to the lysosomes and excretion of excess Cu(+) through lysosomal-mediated exocytosis at the Bile Canaliculus. Here, we show that ATP7B is transported from the trans-Golgi network (TGN) to the Bile Canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu(+) does not cause relocalization of lysosomes and the appearance of lysosome markers in the Bile Canaliculus. Our data reveal the pathway of the Cu(+)-mediated transport of ATP7B from the TGN to the Bile Canaliculus and indicates that the Bile Canaliculus is the primary site of ATP7B action in the elimination of excess Cu(.)

  • Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the Bile Canaliculus.
    Journal of cell science, 2016
    Co-Authors: Vasiliki S. Lalioti, Ramón Peiró, Manuela Pérez-berlanga, Yo Tsuchiya, Angeles Muñoz, Teresa Villalba, Carlos Sánchez, Ignacio V. Sandoval
    Abstract:

    The Cu(+) pump ATP7B plays an irreplaceable role in the elimination of excess Cu(+) by the hepatocyte into the Bile. The trafficking and site of action of ATP7B are subjects of controversy. One current proposal is that an increase in intracellular Cu(+) results in the translocation of ATP7B to the lysosomes and excretion of excess Cu(+) through lysosomal-mediated exocytosis at the Bile Canaliculus. Here, we show that ATP7B is transported from the trans-Golgi network (TGN) to the Bile Canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu(+) does not cause relocalization of lysosomes and the appearance of lysosome markers in the Bile Canaliculus. Our data reveal the pathway of the Cu(+)-mediated transport of ATP7B from the TGN to the Bile Canaliculus and indicates that the Bile Canaliculus is the primary site of ATP7B action in the elimination of excess Cu(.)

D. Cassio - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9. A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one.
    Journal of Cell Science, 1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio
    Abstract:

    By immunofluorescence and freeze fracture methods, we have studied the establishment of hepatic cell polarity in WIF-B9 cells, a subclone of the WIF-B rat hepatoma-derived hybrid cell line. As previously shown (Ihrke et al. (1993) J. Cell Biol. 123, 1761–1775; Shanks et al. (1994) J. Cell Sci. 107, 813–825), these cells are a suitable model for in vitro studies of various hepatic functions, particularly polarity: in confluent cultures, the majority of cells form Bile Canaliculus-like structures; membrane domains are settled, according to plasma membrane protein localization similar to rat hepatocytes in situ. We here report that the establishment of WIF-B9 cell polarity is a slow progressive biphasic phenomenon. During the first days of culture, the majority of cells do not make Bile Canaliculus-like structures. However, they display a polarity similar to that of simple epithelial cells: apical membrane proteins and villin are found at the cell apex; basolateral ones, excluded from this area, are expressed in the remaining membrane area; the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10–15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures.

  • A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one
    1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio, Cnrs Ura
    Abstract:

    the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10-15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures. SUMMARY

C. Decaens - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9. A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one.
    Journal of Cell Science, 1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio
    Abstract:

    By immunofluorescence and freeze fracture methods, we have studied the establishment of hepatic cell polarity in WIF-B9 cells, a subclone of the WIF-B rat hepatoma-derived hybrid cell line. As previously shown (Ihrke et al. (1993) J. Cell Biol. 123, 1761–1775; Shanks et al. (1994) J. Cell Sci. 107, 813–825), these cells are a suitable model for in vitro studies of various hepatic functions, particularly polarity: in confluent cultures, the majority of cells form Bile Canaliculus-like structures; membrane domains are settled, according to plasma membrane protein localization similar to rat hepatocytes in situ. We here report that the establishment of WIF-B9 cell polarity is a slow progressive biphasic phenomenon. During the first days of culture, the majority of cells do not make Bile Canaliculus-like structures. However, they display a polarity similar to that of simple epithelial cells: apical membrane proteins and villin are found at the cell apex; basolateral ones, excluded from this area, are expressed in the remaining membrane area; the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10–15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures.

  • A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one
    1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio, Cnrs Ura
    Abstract:

    the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10-15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures. SUMMARY

Vasiliki S. Lalioti - One of the best experts on this subject based on the ideXlab platform.

  • basolateral sorting and transcytosis define the cu regulated translocation of atp7b to the Bile Canaliculus
    Journal of Cell Science, 2016
    Co-Authors: Vasiliki S. Lalioti, Ramón Peiró, Yo Tsuchiya, Angeles Muñoz, Teresa Villalba, Carlos Sánchez, Manuela Perezberlanga, Ignacio V. Sandoval
    Abstract:

    The Cu(+) pump ATP7B plays an irreplaceable role in the elimination of excess Cu(+) by the hepatocyte into the Bile. The trafficking and site of action of ATP7B are subjects of controversy. One current proposal is that an increase in intracellular Cu(+) results in the translocation of ATP7B to the lysosomes and excretion of excess Cu(+) through lysosomal-mediated exocytosis at the Bile Canaliculus. Here, we show that ATP7B is transported from the trans-Golgi network (TGN) to the Bile Canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu(+) does not cause relocalization of lysosomes and the appearance of lysosome markers in the Bile Canaliculus. Our data reveal the pathway of the Cu(+)-mediated transport of ATP7B from the TGN to the Bile Canaliculus and indicates that the Bile Canaliculus is the primary site of ATP7B action in the elimination of excess Cu(.)

  • Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the Bile Canaliculus.
    Journal of cell science, 2016
    Co-Authors: Vasiliki S. Lalioti, Ramón Peiró, Manuela Pérez-berlanga, Yo Tsuchiya, Angeles Muñoz, Teresa Villalba, Carlos Sánchez, Ignacio V. Sandoval
    Abstract:

    The Cu(+) pump ATP7B plays an irreplaceable role in the elimination of excess Cu(+) by the hepatocyte into the Bile. The trafficking and site of action of ATP7B are subjects of controversy. One current proposal is that an increase in intracellular Cu(+) results in the translocation of ATP7B to the lysosomes and excretion of excess Cu(+) through lysosomal-mediated exocytosis at the Bile Canaliculus. Here, we show that ATP7B is transported from the trans-Golgi network (TGN) to the Bile Canaliculus by basolateral sorting and endocytosis, and microtubule-mediated transcytosis through the subapical compartment. Trafficking ATP7B is not incorporated into lysosomes, and addition of Cu(+) does not cause relocalization of lysosomes and the appearance of lysosome markers in the Bile Canaliculus. Our data reveal the pathway of the Cu(+)-mediated transport of ATP7B from the TGN to the Bile Canaliculus and indicates that the Bile Canaliculus is the primary site of ATP7B action in the elimination of excess Cu(.)

P. Rodriguez - One of the best experts on this subject based on the ideXlab platform.

  • Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9. A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one.
    Journal of Cell Science, 1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio
    Abstract:

    By immunofluorescence and freeze fracture methods, we have studied the establishment of hepatic cell polarity in WIF-B9 cells, a subclone of the WIF-B rat hepatoma-derived hybrid cell line. As previously shown (Ihrke et al. (1993) J. Cell Biol. 123, 1761–1775; Shanks et al. (1994) J. Cell Sci. 107, 813–825), these cells are a suitable model for in vitro studies of various hepatic functions, particularly polarity: in confluent cultures, the majority of cells form Bile Canaliculus-like structures; membrane domains are settled, according to plasma membrane protein localization similar to rat hepatocytes in situ. We here report that the establishment of WIF-B9 cell polarity is a slow progressive biphasic phenomenon. During the first days of culture, the majority of cells do not make Bile Canaliculus-like structures. However, they display a polarity similar to that of simple epithelial cells: apical membrane proteins and villin are found at the cell apex; basolateral ones, excluded from this area, are expressed in the remaining membrane area; the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10–15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures.

  • A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one
    1996
    Co-Authors: C. Decaens, P. Rodriguez, C. Bouchaud, D. Cassio, Cnrs Ura
    Abstract:

    the tight junction-associated protein ZO-1 and actin are concentrated at the boundary of these two poles, whereas E-cadherin is present at the lateral pole just under the apex. With time in culture, the number of cells expressing this simple epithelial polarized phenotype decreases progressively and, after 10-15 days, depending on the plating density, nearly all the cells express the typical hepatic polarized phenotype. The expression of these two phenotypes is mutually exclusive. Freeze-fracture replicas of both types of polarized cells show either macula occludens, fascia occludens (simple epithelial polarity) or zonula occludens (hepatic polarity), associated with gap junctions. In this last case, two or three continuous strands are generally present all around the Bile Canaliculus-like structures. SUMMARY