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Bo Wang - One of the best experts on this subject based on the ideXlab platform.

  • transcriptional data mining of salvia miltiorrhiza in response to methyl jasmonate to examine the mechanism of Bioactive Compound biosynthesis and regulation
    Physiologia Plantarum, 2014
    Co-Authors: Hongmei Luo, Yingjie Zhu, Jingyuan Song, Chao Sun, Xin Zhang, Wei Sun, Bo Wang, Juyan Liu, Shilin Chen
    Abstract:

    Salvia miltiorrhiza is a Chinese herb with significant pharmacologic effects because of the Bioactive Compounds of tanshinones and phenolic acids. Methyl jasmonate (MeJA) has been used as an effective elicitor to enhance the production of these Compounds. However, the molecular mechanism of MeJA-mediated tanshinone and salvianolic acid biosynthesis remains unclear. The transcriptional profiles of S. miltiorrhiza leaves at 12 h (T12) after MeJA elicitation and mock-treated leaves (T0) were generated using the Illumina deep RNA sequencing (RNA-seq) strategy to detect the changes in gene expression in response to MeJA. In total, 37 647 unique sequences were obtained from about 21 million reads, and 25 641 (71.53%) of these sequences were annotated based on the blast searches against the public databases. A total of 5287 unique sequences were expressed differentially between the samples of T0 and T12, which covered almost all the known genes involved in tanshinone and phenolic acid biosynthesis in S. miltiorrhiza. Many of the transcription factors (e.g. MYB, bHLH and WRKY) and genes involved in plant hormone biosynthesis and signal transduction were expressed differentially in response to the MeJA induction. Importantly, three and four candidate cytochrome P450s (P450s) that could be involved in the tanshinone and phenolic acid biosynthesis, respectively, were selected from the RNA-seq data based on co-expressed pattern analysis with SmCPS1/SmKSL1 and SmRAS, which are the key genes responsible for biosynthesis. This comprehensive investigation of MeJA-induced gene expression profiles can shed light on the molecular mechanisms of the MeJA-mediated Bioactive Compound biosynthesis and regulation in S. miltiorrhiza.

  • emulsions stabilized by mini cyclic proteins for Bioactive Compound delivery
    RSC Advances, 2014
    Co-Authors: Bo Wang, Yuan Lin, Ninghua Tan, Qian Wang
    Abstract:

    Mini cyclic proteins, members of the family of cyclotides, can stabilize oil-in-water emulsions by forming a single layer assembly at the oil–water interface. Such emulsions can be potentially employed to deliver hydrophobic Bioactive cargos.

P Venkatachalam - One of the best experts on this subject based on the ideXlab platform.

  • Bioactive Compound loaded stable silver nanoparticle synthesis from microwave irradiated aqueous extracellular leaf extracts of naringi crenulata and its wound healing activity in experimental rat model
    Acta Tropica, 2014
    Co-Authors: T Bhuvaneswari, M Thiyagarajan, N P Geetha, P Venkatachalam
    Abstract:

    An efficient and eco-friendly protocol for the synthesis of Bioactive silver nanoparticles was developed using Naringi crenulata leaf extracts via microwave irradiation method. Silver nanoparticles were synthesized by treating N. crenulata leaf extracts with 1mM of aqueous silver nitrate solution. An effective Bioactive Compound such as alkaloids, phenols, saponins and quinines present in the N. crenulata reduces the Ag(+) into Ag(0). The synthesized silver nanoparticles were monitored by UV-vis spectrophotometer and further characterized by X-ray diffraction (XRD), Fourier Transform Infra Red (FTIR), Energy-dispersive X-ray spectroscopy (EDX) and field emission scanning electron microscopy (FESEM). UV-vis spectroscopy showed maximum absorbance at 390nm due to surface plasmon resonance of AgNPs. From FESEM results, an average crystal size of the synthesized nanoparticle was 72-98nm. FT-IR results showed sharp absorption peaks and they were assigned to phosphine, alkyl halides and sulfonate groups. Silver nanoparticles synthesized were generally found to be spherical and cubic shape. Topical application of ointment prepared from silver nanoparticles of N. crenulata were formulated and evaluated in vivo using the excision wound healing model on Wistar albino rats. The measurement of the wound areas was performed on 3rd, 6th, 9th, 12th and 15th days and the percentage of wound closures was calculated accordingly. By the 15th day, the ointment base containing 5% (w/w) of silver nanoparticles showed 100% wound healing activity compared with that of the reference as well as control bases. The results strongly suggested that the batch C ointment containing silver nanaoparticles synthesized from the leaf extracts of N. crenulata was found to be very effective in wound repair and encourages harnessing the potentials of the plant biomolecules loaded silver nanoparticle in the treatment of tropical diseases including wound healing.

Qian Wang - One of the best experts on this subject based on the ideXlab platform.

Renan Campos Chiste - One of the best experts on this subject based on the ideXlab platform.

  • biodegradable polymers as wall materials to the synthesis of Bioactive Compound nanocapsules
    Trends in Food Science and Technology, 2016
    Co-Authors: Priscilla Pereira Dos Santos, Simone Hickmann Flores, Alessandro De Oliveira Rios, Renan Campos Chiste
    Abstract:

    Abstract Background Nanoparticles have been synthetized using polymers as wall materials to protect Bioactive Compounds against external factors (light, heat and oxygen), increasing the stability and improving the bioavailability of nanoencapsulated Compounds. Scope and approach The encapsulation processes and type of polymers (natural or synthetic) exert a direct impact on the synthesis of Bioactive Compound nanocapsules, which reflect in parameters, such as size, zeta potential, encapsulation efficiency, aqueous solubility, aqueous stability, surface permeability, desired Bioactive Compounds release profile and wall resistance; and these characteristics might limit its use by food, pharmaceutical and cosmetic industries. This review summarizes researches on nanocapsules synthesis (advantages and limitations of different techniques) and focuses on the importance of different biodegradable polymers as wall materials for obtaining stable and safe nanocapsules. Key findings and conclusions Different wall materials can be used to synthesize Bioactive Compound nanocapsules; however, biodegradable polymeric nanocapsules have proven to be one of the most stable structures during storage and showed high efficiency to control the release of encapsulated Compounds and due to these characteristics, they have been focus of various studies for future applications in health and food-related areas.

Kee-yoeup Paek - One of the best experts on this subject based on the ideXlab platform.

  • Enhancement strategies of Bioactive Compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors
    Plant Cell Tissue and Organ Culture (PCTOC), 2015
    Co-Authors: Eunjung Lee, So Young Park, Kee-yoeup Paek
    Abstract:

    To establish an attractive method for the annual production of valuable Eleutherococcus koreanum -based Bioactive Compounds, the adventitious roots in airlift bioreactors were subjected to methyl jasmonate (MJ) and salicylic acid (SA) treatment for 1 week before harvest as a chemical elicitor to enhance the productivity of Bioactive Compounds. After 6 weeks of culture, the addition of 50 µmol MJ was found to be the optimal concentration and elicitor type for biomass and Bioactive Compound production in the roots, as evidenced by the highest biomass and total production of five target Bioactive Compounds (i.e., eleutherosides B and E, chlorogenic acid, and total phenolics and flavonoids) without causing any physiological disorders. The total production of the target Bioactive Compounds at 50 µmol MJ (303.93 mg L^−1) was 37.77 % higher than that for the control treatment, and the addition of high concentrations of MJ and SA increased 2,2-diphenyl-1-picrylhydrazyl activity and hydrogen peroxide content in the roots. In addition, the adventitious roots subjected to 50 µmol MJ for 1 week had a competitive edge for commercial use, as evidenced by the high total production of the five target Bioactive Compounds (per 1 L of medium) when compared to the 3-year field-grown plants.

  • effect of nitrogen source on biomass and Bioactive Compound production in submerged cultures of eleutherococcus koreanum nakai adventitious roots
    Biotechnology Progress, 2012
    Co-Authors: Eunjung Lee, Kee-yoeup Paek
    Abstract:

    Ammonium to nitrate ratios of 0:30, 5:25, 10:20, 15:15, 20:10, 25:5, and 30:0 mM were tested to determine the optimal NH4+:NO3− ratio for improving biomass and Bioactive Compound production in Eleutherococcus koreanum Nakai adventitious roots using 3-L bulb-type bubble bioreactors. A high ammonium nitrogen ratio had a negative effect on root growth, and the highest fresh and dry weights were obtained when NH4+:NO3− ratios were 5:25 and 10:20 (mM) after 5 weeks of culture. Although the total production of eleutherosides B and E was slightly higher at the 10:20 ratio than at the 5:25 ratio (NH4+:NO3−), we proposed that the optimal NH4+:NO3− ratio was 5:25 mM. This ratio achieved both the highest total production of five target Bioactive Compounds (eleutherosides B and E, chlorogenic acid, total phenolics, and flavonoids) and the highest root biomass. Furthermore, increasing NH4+:NO3− ratios to 10:20 decreased pH in the medium, interrupted the absorption of essential minerals from the culture medium, and resulted in low biomass and increased relative oxidative stress levels, which were evaluated by determining 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Therefore, nitrate rather than ammonium nitrogen was more essential not for only biomass production but also for Bioactive Compound production in E. koreanum adventitious root cultures. The optimal nitrogen source ratio produced 5.63 g L−1 of biomass and 24.41 mg of the five total Bioactive Compounds per gram of biomass (dry weight basis). The development of such in vitro culture technology will benefit the pilot-scale production of E. koreanum-based Bioactive Compounds for commercialization. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011

  • influence of inoculum density and aeration volume on biomass and Bioactive Compound production in bulb type bubble bioreactor cultures of eleutherococcus koreanum nakai
    Bioresource Technology, 2011
    Co-Authors: Eunjung Lee, Sang Hyun Moh, Kee-yoeup Paek
    Abstract:

    This study deals with the effects of initial inoculum density and aeration volume on biomass and Bioactive Compound production in adventitious roots of Eleutherococcus koreanum Nakai in bulb-type bubble bioreactors (3-L capacity). While the fresh and dry weights of the roots increased with increasing inoculum density, the highest percentage dry weight and accumulation of total target Compounds (eleutheroside B and E, chlorogenic acid, total phenolics, and flavonoids) were noted at an inoculum density of 5.0 g L(-1). Poor aeration volume (0.05 vvm) stunted root growth, and high aeration volume (0.4 vvm) caused physiological disorders. Moreover, an inoculum density of 5.0 g L(-1) and an aeration volume of 0.1 vvm resulted in the highest concentration of total target Compounds and least root death. Such optimization of culture conditions will be beneficial for the large-scale production of E. koreanum biomass and Bioactive Compounds.

  • auxin and cytokinin affect biomass and Bioactive Compound production from adventitious roots of eleutherococcus koreanum
    Korean Journal of Horticultural Science & Technology, 2010
    Co-Authors: Eunjung Lee, Myongki Kim, Kee-yoeup Paek
    Abstract:

    In an attempt to improve biomass and Bioactive Compound production, we cultured adventitious roots of Eleutherococcus koreanum in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at 5 mg·L -1 indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with 5 mg·L -1 IBA compared to -naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of 5 mg·L -1 IBA with 0.1 mg·L -1