The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform
Fan Yang - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and Mechanical Gradients Synergize To Enhance Cartilage Zonal Organization in 3D.
ACS biomaterials science & engineering, 2018Co-Authors: Danqing Zhu, Pavin Trinh, Elisa Liu, Fan YangAbstract:Articular cartilage is characterized by zonal organizations containing dual gradients of Biochemical Cues and mechanical Cues. However, how Biochemical gradient interacts with the mechanical gradient to drive the cartilage zonal development remains largely unknown. Here, we report the development of a dual-gradient hydrogel platform as a 3D niche to elucidate the relative contributions of Biochemical and mechanical niche gradients in modulating zonal-specific chondrocyte responses and cartilage zonal organization. Chondroitin sulfate (CS), a major constituent of cartilage extracellular matrix, was chosen as the Biochemical Cue. Poly(ethylene glycol), a bioinert polymer, was used to create the stiffness gradient. Dual-gradient hydrogels upregulated cartilage marker expressions and increased chondrocyte proliferation and collagen deposition in a zonal-dependent manner. Hydrogels with CS gradient alone exhibited poor mechanical strength and degraded prematurely after 1 week of culture. While CS gradient alon...
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Biochemical and Mechanical Gradients Synergize To Enhance Cartilage Zonal Organization in 3D
2018Co-Authors: Danqing Zhu, Pavin Trinh, Elisa Liu, Fan YangAbstract:Articular cartilage is characterized by zonal organizations containing dual gradients of Biochemical Cues and mechanical Cues. However, how Biochemical gradient interacts with the mechanical gradient to drive the cartilage zonal development remains largely unknown. Here, we report the development of a dual-gradient hydrogel platform as a 3D niche to elucidate the relative contributions of Biochemical and mechanical niche gradients in modulating zonal-specific chondrocyte responses and cartilage zonal organization. Chondroitin sulfate (CS), a major constituent of cartilage extracellular matrix, was chosen as the Biochemical Cue. Poly(ethylene glycol), a bioinert polymer, was used to create the stiffness gradient. Dual-gradient hydrogels upregulated cartilage marker expressions and increased chondrocyte proliferation and collagen deposition in a zonal-dependent manner. Hydrogels with CS gradient alone exhibited poor mechanical strength and degraded prematurely after 1 week of culture. While CS gradient alone did not support long-term culture, adding CS gradient to mechanical-gradient hydrogels substantially enhanced cell proliferation, glycosaminoglycan production, and collagen deposition compared to mechanical-gradient hydrogels alone. These results suggest that Biochemical and mechanical gradient Cues synergize to enhance cartilage zonal organization by chondrocytes in 3D. Together, our results validate the potential of dual-gradient hydrogels as a 3D cell niche for cartilage regeneration with zonal organization and may be used to recreate other tissue interfaces
Marleen Kamperman - One of the best experts on this subject based on the ideXlab platform.
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fibrous hydrogels for cell encapsulation a modular and supramolecular approach
PLOS ONE, 2016Co-Authors: Malgorzata K Wlodarczykbiegun, Kambiz Farbod, Marc W T Werten, Cornelis J Slingerland, Sander C G Leeuwenburgh, Martien Cohen A Stuart, Frits A De Wolf, Jeroen J J P Van Den Beucken, Marleen KampermanAbstract:Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and Biochemical Cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the Biochemical Cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and Biochemical Cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions.
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fibrous hydrogels for cell encapsulation
PLOS ONE, 2016Co-Authors: Malgorzata K Wlodarczykbiegun, Kambiz Farbod, Marc W T Werten, Cornelis J Slingerland, De Frits A Wolf, Van Den Jeroen J J P Beucken, Sander C G Leeuwenburgh, Martien Cohen A Stuart, Marleen KampermanAbstract:Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and Biochemical Cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the Biochemical Cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and Biochemical Cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions.
Danqing Zhu - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and Mechanical Gradients Synergize To Enhance Cartilage Zonal Organization in 3D.
ACS biomaterials science & engineering, 2018Co-Authors: Danqing Zhu, Pavin Trinh, Elisa Liu, Fan YangAbstract:Articular cartilage is characterized by zonal organizations containing dual gradients of Biochemical Cues and mechanical Cues. However, how Biochemical gradient interacts with the mechanical gradient to drive the cartilage zonal development remains largely unknown. Here, we report the development of a dual-gradient hydrogel platform as a 3D niche to elucidate the relative contributions of Biochemical and mechanical niche gradients in modulating zonal-specific chondrocyte responses and cartilage zonal organization. Chondroitin sulfate (CS), a major constituent of cartilage extracellular matrix, was chosen as the Biochemical Cue. Poly(ethylene glycol), a bioinert polymer, was used to create the stiffness gradient. Dual-gradient hydrogels upregulated cartilage marker expressions and increased chondrocyte proliferation and collagen deposition in a zonal-dependent manner. Hydrogels with CS gradient alone exhibited poor mechanical strength and degraded prematurely after 1 week of culture. While CS gradient alon...
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Biochemical and Mechanical Gradients Synergize To Enhance Cartilage Zonal Organization in 3D
2018Co-Authors: Danqing Zhu, Pavin Trinh, Elisa Liu, Fan YangAbstract:Articular cartilage is characterized by zonal organizations containing dual gradients of Biochemical Cues and mechanical Cues. However, how Biochemical gradient interacts with the mechanical gradient to drive the cartilage zonal development remains largely unknown. Here, we report the development of a dual-gradient hydrogel platform as a 3D niche to elucidate the relative contributions of Biochemical and mechanical niche gradients in modulating zonal-specific chondrocyte responses and cartilage zonal organization. Chondroitin sulfate (CS), a major constituent of cartilage extracellular matrix, was chosen as the Biochemical Cue. Poly(ethylene glycol), a bioinert polymer, was used to create the stiffness gradient. Dual-gradient hydrogels upregulated cartilage marker expressions and increased chondrocyte proliferation and collagen deposition in a zonal-dependent manner. Hydrogels with CS gradient alone exhibited poor mechanical strength and degraded prematurely after 1 week of culture. While CS gradient alone did not support long-term culture, adding CS gradient to mechanical-gradient hydrogels substantially enhanced cell proliferation, glycosaminoglycan production, and collagen deposition compared to mechanical-gradient hydrogels alone. These results suggest that Biochemical and mechanical gradient Cues synergize to enhance cartilage zonal organization by chondrocytes in 3D. Together, our results validate the potential of dual-gradient hydrogels as a 3D cell niche for cartilage regeneration with zonal organization and may be used to recreate other tissue interfaces
Malgorzata K Wlodarczykbiegun - One of the best experts on this subject based on the ideXlab platform.
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fibrous hydrogels for cell encapsulation a modular and supramolecular approach
PLOS ONE, 2016Co-Authors: Malgorzata K Wlodarczykbiegun, Kambiz Farbod, Marc W T Werten, Cornelis J Slingerland, Sander C G Leeuwenburgh, Martien Cohen A Stuart, Frits A De Wolf, Jeroen J J P Van Den Beucken, Marleen KampermanAbstract:Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and Biochemical Cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the Biochemical Cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and Biochemical Cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions.
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fibrous hydrogels for cell encapsulation
PLOS ONE, 2016Co-Authors: Malgorzata K Wlodarczykbiegun, Kambiz Farbod, Marc W T Werten, Cornelis J Slingerland, De Frits A Wolf, Van Den Jeroen J J P Beucken, Sander C G Leeuwenburgh, Martien Cohen A Stuart, Marleen KampermanAbstract:Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and Biochemical Cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the Biochemical Cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and Biochemical Cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions.
Gang-yu Liu - One of the best experts on this subject based on the ideXlab platform.
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Nanogratings of fibronectin provide an effective Biochemical Cue for regulating focal adhesion and cellular structure
Nano Research, 2012Co-Authors: Lifang Shi, Yi Ping Shih, Gang-yu LiuAbstract:Integrin clustering, typically nanometers in dimension, is the first and an important step in integrin-mediated cellular signaling processes such as focal adhesion. Engineered nanostructures mimicking extracellular matrices (ECM) provide a new approach for investigation and regulation of this initial step and of downstream cascades of focal adhesion. This work reveals that fibronectin (Fn) nanostructures, even at a small height of 3.2 nm ± 0.5 nm, exhibit high efficacy in guiding cellular orientation and polarization. More interestingly, the Fn nanostructures also impact intracellular structures such as preferential filopodia attachment, and commensurate alignment of intracellular actin stress fibers. The impact can be rationalized by the strong and specific interaction between integrin and Fn, leading to integrin clusters and then focal adhesion assemblies following the underlying nanostructure of Fn. This guided assembly further mediates the downstream behavior, such as actin stress fiber alignment and overall cellular morphology. Our observations collectively demonstrate that engineered nanostructures of Fn provide an alternative and high efficacy Biochemical Cue for regulation of cellular signaling processes. Open image in new window