The Experts below are selected from a list of 70890 Experts worldwide ranked by ideXlab platform
Jay L Zweier - One of the best experts on this subject based on the ideXlab platform.
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theoretical and experimental studies of the spin trapping of inorganic radicals by 5 5 dimethyl 1 pyrroline n oxide dmpo 2 carbonate radical anion
Journal of Physical Chemistry A, 2006Co-Authors: Frederick A Villamena, Edward J Locigno, Antal Rockenbauer, Christopher M Hadad, Jay L ZweierAbstract:The carbon dioxide radical anion (CO2•-) is known to be generated in vivo through various chemical and Biochemical Pathways. Electron paramagnetic resonance (EPR) spin trapping with the commonly us...
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theoretical and experimental studies of the spin trapping of inorganic radicals by 5 5 dimethyl 1 pyrroline n oxide dmpo 1 carbon dioxide radical anion
Journal of Physical Chemistry A, 2006Co-Authors: Frederick A Villamena, Edward J Locigno, Antal Rockenbauer, Christopher M Hadad, Jay L ZweierAbstract:The carbon dioxide radical anion (CO2•-) is known to be generated in vivo through various chemical and Biochemical Pathways. Electron paramagnetic resonance (EPR) spin trapping with the commonly us...
Alison M Derry - One of the best experts on this subject based on the ideXlab platform.
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oxic water column methanogenesis as a major component of aquatic ch4 fluxes
Nature Communications, 2014Co-Authors: Matthew J Bogard, Paul A Del Giorgio, Lennie Boutet, Maria Carolina Garcia Chaves, Anthony Merante, Yves T Prairie, Alison M DerryAbstract:Aquatic methane originates in anoxic sediments and bottom waters, but some studies suggest it may also come from the oxic water column. Here, the authors experimentally confirm this hypothesis, and explore the rates, controls and Biochemical Pathways of oxic water methanogenesis.
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oxic water column methanogenesis as a major component of aquatic ch 4 fluxes
Nature Communications, 2014Co-Authors: Matthew J Bogard, Lennie Boutet, Maria Carolina Garcia Chaves, Anthony Merante, Yves T Prairie, Paul A Del Giorgio, Alison M DerryAbstract:Aquatic methane originates in anoxic sediments and bottom waters, but some studies suggest it may also come from the oxic water column. Here, the authors experimentally confirm this hypothesis, and explore the rates, controls and Biochemical Pathways of oxic water methanogenesis.
Huimin Zhao - One of the best experts on this subject based on the ideXlab platform.
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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large Biochemical Pathways in Saccharomyces cerevisiae
Metabolic Engineering, 2016Co-Authors: Shuobo Shi, Youyun Liang, Ee Lui Ang, Mingzi M. Zhang, Huimin ZhaoAbstract:Despite recent advances in genome editing capabilities for the model organism Saccharomyces cerevisiae, the chromosomal integration of large Biochemical Pathways for stable industrial production remains challenging. In this work, we developed a simple platform for high-efficiency, single-step, markerless, multi-copy chromosomal integration of full Biochemical Pathways in Saccharomyces cerevisiae. In this Di-CRISPR (delta integration CRISPR-Cas) platform based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated systems (Cas), we specifically designed guide RNA sequences to target multiple delta sites in the yeast genome. The generation of double stranded breaks at the delta sites allowed simultaneous integration of multiple copies of linearized donor DNA containing large Biochemical Pathways. With our newly developed Di-CRISPR platform, we were able to attain highly efficient and markerless integration of large Biochemical Pathways and achieve an unprecedented 18-copy genomic integration of a 24 kb combined xylose utilization and (R,R)-2,3-butanediol (BDO) production pathway in a single step, thus generating a strain that was able to produce BDO directly from xylose. The simplicity and high efficiency of the Di-CRISPR platform could provide a superior alternative to high copy plasmids and would render this platform an invaluable tool for genome editing and metabolic engineering in S. cerevisiae.
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construction and engineering of large Biochemical Pathways via dna assembler
Methods of Molecular Biology, 2013Co-Authors: Zengyi Shao, Huimin ZhaoAbstract:DNA assembler enables rapid construction and engineering of Biochemical Pathways in a one-step fashion by exploitation of the in vivo homologous recombination mechanism in Saccharomyces cerevisiae. It has many applications in pathway engineering, metabolic engineering, combinatorial biology, and synthetic biology. Here we use two examples including the zeaxanthin biosynthetic pathway and the aureothin biosynthetic gene cluster to describe the key steps in the construction of Pathways containing multiple genes using the DNA assembler approach. Methods for construct design, pathway assembly, pathway confirmation, and functional analysis are shown. The protocol for fine genetic modifications such as site-directed mutagenesis for engineering the aureothin gene cluster is also illustrated.
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dna assembler an in vivo genetic method for rapid construction of Biochemical Pathways
Nucleic Acids Research, 2009Co-Authors: Zengyi Shao, Hua Zhao, Huimin ZhaoAbstract:The assembly of large recombinant DNA encoding a whole Biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire Biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional D-xylose utilization pathway (~9kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (~11kb DNA consisting of five genes) and a functional combined D-xylose utilization and zeaxanthin biosynthesis pathway (~19kb consisting of eight genes) with high efficiencies (70–100%) either on a plasmid or on a yeast chromosome. As this new method only requires simple DNA preparation and one-step yeast transformation, it represents a powerful tool in the construction of Biochemical Pathways for synthetic biology, metabolic engineering and functional genomics studies.
Frederick A Villamena - One of the best experts on this subject based on the ideXlab platform.
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theoretical and experimental studies of the spin trapping of inorganic radicals by 5 5 dimethyl 1 pyrroline n oxide dmpo 2 carbonate radical anion
Journal of Physical Chemistry A, 2006Co-Authors: Frederick A Villamena, Edward J Locigno, Antal Rockenbauer, Christopher M Hadad, Jay L ZweierAbstract:The carbon dioxide radical anion (CO2•-) is known to be generated in vivo through various chemical and Biochemical Pathways. Electron paramagnetic resonance (EPR) spin trapping with the commonly us...
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theoretical and experimental studies of the spin trapping of inorganic radicals by 5 5 dimethyl 1 pyrroline n oxide dmpo 1 carbon dioxide radical anion
Journal of Physical Chemistry A, 2006Co-Authors: Frederick A Villamena, Edward J Locigno, Antal Rockenbauer, Christopher M Hadad, Jay L ZweierAbstract:The carbon dioxide radical anion (CO2•-) is known to be generated in vivo through various chemical and Biochemical Pathways. Electron paramagnetic resonance (EPR) spin trapping with the commonly us...
Matthew J Bogard - One of the best experts on this subject based on the ideXlab platform.
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oxic water column methanogenesis as a major component of aquatic ch4 fluxes
Nature Communications, 2014Co-Authors: Matthew J Bogard, Paul A Del Giorgio, Lennie Boutet, Maria Carolina Garcia Chaves, Anthony Merante, Yves T Prairie, Alison M DerryAbstract:Aquatic methane originates in anoxic sediments and bottom waters, but some studies suggest it may also come from the oxic water column. Here, the authors experimentally confirm this hypothesis, and explore the rates, controls and Biochemical Pathways of oxic water methanogenesis.
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oxic water column methanogenesis as a major component of aquatic ch 4 fluxes
Nature Communications, 2014Co-Authors: Matthew J Bogard, Lennie Boutet, Maria Carolina Garcia Chaves, Anthony Merante, Yves T Prairie, Paul A Del Giorgio, Alison M DerryAbstract:Aquatic methane originates in anoxic sediments and bottom waters, but some studies suggest it may also come from the oxic water column. Here, the authors experimentally confirm this hypothesis, and explore the rates, controls and Biochemical Pathways of oxic water methanogenesis.