The Experts below are selected from a list of 5925 Experts worldwide ranked by ideXlab platform

Gabert Heidrich - One of the best experts on this subject based on the ideXlab platform.

Katrin G. Heinze - One of the best experts on this subject based on the ideXlab platform.

  • Sharpening emitter localization in front of a tuned mirror
    Light science & applications, 2018
    Co-Authors: Hannah S. Heil, Benjamin Schreiber, Monika Emmerling, Sven Höfling, Martin Kamp, Markus Sauer, Ralph Götz, Marie-christine Dabauvalle, Georg Krohne, Katrin G. Heinze
    Abstract:

    Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a Metal-dielectric nanocoating that acts as a tuned mirror2-4. Here, we demonstrate that a higher photon yield at a lower background on Biocompatible Metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

  • Boosting the localization precision of dSTORM by Biocompatible Metal-dielectric coated glass coverslips
    2017
    Co-Authors: Hannah S. Heil, Benjamin Schreiber, Monika Emmerling, Sven Höfling, Martin Kamp, Markus Sauer, Katrin G. Heinze
    Abstract:

    Super-resolution techniques such as direct Stochastic Optical Reconstruction Microscopy (dSTORM) have become versatile and well-established tools for biological imaging over the last century. Here, we theoretically and experimentally show that clever combination of different fluorescence modalities allows further improvements. We found that the interaction of fluorophores with plasmonic surfaces boost super-resolution performance in dSTORM approaches as it allows for tailoring the excitation and emission properties. The strength of the approach is that no further specialized microscope setup is required as the described enhancement solely rely on Metal-dielectric coated glass coverslips that are straightforward to fabricate. Such Biocompatible plasmonic nanolayers enhance the signal-to-noise ratio of dSTORM, and thus sharpens the localization precision by a factor of two.

  • spectrally coded optical nanosectioning specon with Biocompatible Metal dielectric coated substrates
    Proceedings of the National Academy of Sciences of the United States of America, 2013
    Co-Authors: Kareem Elsayad, Alexander Urich, Piau Siong Tan, Maria Nemethova, J.v. Small, Karl Unterrainer, Katrin G. Heinze
    Abstract:

    Fluorescence nanosectioning within a submicron region above an interface is desirable for many disciplines in the life sciences. A drawback, however, to most current approaches is the a priori need to physically scan a sculptured point spread function in the axial dimension, which can be undesirable for optically sensitive or highly dynamic samples. Here we demonstrate a fluorescence imaging approach that can overcome the need for scanning by exploiting the position-dependent emission spectrum of fluorophores above a simple Biocompatible nanostructure. To achieve this we have designed a thin Metal–dielectric-coated substrate, where the spectral modification to the total measured fluorescence can be used to estimate the axial fluorophore distribution within distances of 10–150 nm above the substrate with an accuracy of up to 5–10 nm. The modeling and feasibility of the approach are verified and successfully applied to elucidate nanoscale adhesion protein and filopodia dynamics in migrating cells. It is likely that the general principle can find broader applications in, for example, single-molecule studies, biosensing, and studying fast dynamic processes.

  • Spectrally coded optical nanosectioning (SpecON) with Biocompatible Metal–dielectric-coated substrates
    Proceedings of the National Academy of Sciences of the United States of America, 2013
    Co-Authors: Kareem Elsayad, Alexander Urich, Piau Siong Tan, Maria Nemethova, J.v. Small, Karl Unterrainer, Katrin G. Heinze
    Abstract:

    Fluorescence nanosectioning within a submicron region above an interface is desirable for many disciplines in the life sciences. A drawback, however, to most current approaches is the a priori need to physically scan a sculptured point spread function in the axial dimension, which can be undesirable for optically sensitive or highly dynamic samples. Here we demonstrate a fluorescence imaging approach that can overcome the need for scanning by exploiting the position-dependent emission spectrum of fluorophores above a simple Biocompatible nanostructure. To achieve this we have designed a thin Metal–dielectric-coated substrate, where the spectral modification to the total measured fluorescence can be used to estimate the axial fluorophore distribution within distances of 10–150 nm above the substrate with an accuracy of up to 5–10 nm. The modeling and feasibility of the approach are verified and successfully applied to elucidate nanoscale adhesion protein and filopodia dynamics in migrating cells. It is likely that the general principle can find broader applications in, for example, single-molecule studies, biosensing, and studying fast dynamic processes.

Thorsten Ernstberger - One of the best experts on this subject based on the ideXlab platform.

Gottfried H. Buchhorn - One of the best experts on this subject based on the ideXlab platform.

Kareem Elsayad - One of the best experts on this subject based on the ideXlab platform.

  • spectrally coded optical nanosectioning specon with Biocompatible Metal dielectric coated substrates
    Proceedings of the National Academy of Sciences of the United States of America, 2013
    Co-Authors: Kareem Elsayad, Alexander Urich, Piau Siong Tan, Maria Nemethova, J.v. Small, Karl Unterrainer, Katrin G. Heinze
    Abstract:

    Fluorescence nanosectioning within a submicron region above an interface is desirable for many disciplines in the life sciences. A drawback, however, to most current approaches is the a priori need to physically scan a sculptured point spread function in the axial dimension, which can be undesirable for optically sensitive or highly dynamic samples. Here we demonstrate a fluorescence imaging approach that can overcome the need for scanning by exploiting the position-dependent emission spectrum of fluorophores above a simple Biocompatible nanostructure. To achieve this we have designed a thin Metal–dielectric-coated substrate, where the spectral modification to the total measured fluorescence can be used to estimate the axial fluorophore distribution within distances of 10–150 nm above the substrate with an accuracy of up to 5–10 nm. The modeling and feasibility of the approach are verified and successfully applied to elucidate nanoscale adhesion protein and filopodia dynamics in migrating cells. It is likely that the general principle can find broader applications in, for example, single-molecule studies, biosensing, and studying fast dynamic processes.

  • Spectrally coded optical nanosectioning (SpecON) with Biocompatible Metal–dielectric-coated substrates
    Proceedings of the National Academy of Sciences of the United States of America, 2013
    Co-Authors: Kareem Elsayad, Alexander Urich, Piau Siong Tan, Maria Nemethova, J.v. Small, Karl Unterrainer, Katrin G. Heinze
    Abstract:

    Fluorescence nanosectioning within a submicron region above an interface is desirable for many disciplines in the life sciences. A drawback, however, to most current approaches is the a priori need to physically scan a sculptured point spread function in the axial dimension, which can be undesirable for optically sensitive or highly dynamic samples. Here we demonstrate a fluorescence imaging approach that can overcome the need for scanning by exploiting the position-dependent emission spectrum of fluorophores above a simple Biocompatible nanostructure. To achieve this we have designed a thin Metal–dielectric-coated substrate, where the spectral modification to the total measured fluorescence can be used to estimate the axial fluorophore distribution within distances of 10–150 nm above the substrate with an accuracy of up to 5–10 nm. The modeling and feasibility of the approach are verified and successfully applied to elucidate nanoscale adhesion protein and filopodia dynamics in migrating cells. It is likely that the general principle can find broader applications in, for example, single-molecule studies, biosensing, and studying fast dynamic processes.