The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Masataka Sata - One of the best experts on this subject based on the ideXlab platform.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:OBJECTIVE: There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those <5 mm in diameter, are associated with a high incidence of thrombosis. Fibroin is a Biodegradable protein derived from silk. Silk fibroin from Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. METHODS: A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. RESULTS: The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P < .01). Endothelial cells and smooth muscle cells (SMCs) migrated into the fibroin graft early after implantation and became organized into endothelial and medial layers, as determined by anti-CD31 and anti-alpha-smooth muscle actin immunostaining. The total number of SMCs increased 1.6-fold from 1 month to 3 months. Vasa vasorum also formed in the adventitia. Sirius red staining of the fibroin grafts revealed that the content of collagen significantly increased at 1 year after implantation, with a decrease in fibroin content. GFP-positive cells contributed to organization of a smooth muscle layer. CONCLUSIONS: Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
Makoto Sumi - One of the best experts on this subject based on the ideXlab platform.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:OBJECTIVE: There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those <5 mm in diameter, are associated with a high incidence of thrombosis. Fibroin is a Biodegradable protein derived from silk. Silk fibroin from Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. METHODS: A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. RESULTS: The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P < .01). Endothelial cells and smooth muscle cells (SMCs) migrated into the fibroin graft early after implantation and became organized into endothelial and medial layers, as determined by anti-CD31 and anti-alpha-smooth muscle actin immunostaining. The total number of SMCs increased 1.6-fold from 1 month to 3 months. Vasa vasorum also formed in the adventitia. Sirius red staining of the fibroin grafts revealed that the content of collagen significantly increased at 1 year after implantation, with a decrease in fibroin content. GFP-positive cells contributed to organization of a smooth muscle layer. CONCLUSIONS: Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
Soichiro Enomoto - One of the best experts on this subject based on the ideXlab platform.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:OBJECTIVE: There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those <5 mm in diameter, are associated with a high incidence of thrombosis. Fibroin is a Biodegradable protein derived from silk. Silk fibroin from Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. METHODS: A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. RESULTS: The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P < .01). Endothelial cells and smooth muscle cells (SMCs) migrated into the fibroin graft early after implantation and became organized into endothelial and medial layers, as determined by anti-CD31 and anti-alpha-smooth muscle actin immunostaining. The total number of SMCs increased 1.6-fold from 1 month to 3 months. Vasa vasorum also formed in the adventitia. Sirius red staining of the fibroin grafts revealed that the content of collagen significantly increased at 1 year after implantation, with a decrease in fibroin content. GFP-positive cells contributed to organization of a smooth muscle layer. CONCLUSIONS: Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
Junkyo Suh - One of the best experts on this subject based on the ideXlab platform.
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gag augmented polysaccharide hydrogel a novel biocompatible and Biodegradable Material to support chondrogenesis
Journal of Biomedical Materials Research, 2000Co-Authors: Franklin V Sechriest, Yi Jian Miao, Christopher Niyibizi, Andrea Westerhausenlarson, Howard W T Matthew, Christopher H Evans, Junkyo SuhAbstract:The quality of articular cartilage engineered using a cell–polymer construct depends, in part, on the chemical composition of the bioMaterial and whether that bioMaterial can support the chondrocytic phenotype. Acknowledging the supportive influence of tissue-specific matrix molecules on the chondrocytic phenotype, we have combined chondroitin sulfate-A (CSA) and chitosan, a glycosaminoglycan (GAG) analog, to develop a novel bioMaterial to support chondrogenesis. Chitosan is a polycationic repeating monosaccharide of β-1,4-linked glucosamine monomers with randomly located N-acetyl glucosamine units. Chitosan may be combined with the polyanionic CSA such that ionic crosslinking results in hydrogel formation. Bovine primary articular chondrocytes, when seeded onto a thin layer of CSA–chitosan, form discrete, focal adhesions to the Material and maintain many characteristics of the differentiated chondrocytic phenotype, including round morphology, limited mitosis, collagen type II, and proteoglycan production. Our findings suggest CSA–chitosan may be well suited as a carrier Material for the transplant of autologous chondrocytes or as a scaffold for the tissue engineering of cartilage-like tissue. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 49, 534–541, 2000.
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gag augmented polysaccharide hydrogel a novel biocompatible and Biodegradable Material to support chondrogenesis
Journal of Biomedical Materials Research, 2000Co-Authors: Franklin V Sechriest, Yi Jian Miao, Christopher Niyibizi, Andrea Westerhausenlarson, Howard W T Matthew, Christopher H Evans, Junkyo SuhAbstract:The quality of articular cartilage engineered using a cell-polymer construct depends, in part, on the chemical composition of the bioMaterial and whether that bioMaterial can support the chondrocytic phenotype. Acknowledging the supportive influence of tissue-specific matrix molecules on the chondrocytic phenotype, we have combined chondroitin sulfate-A (CSA) and chitosan, a glycosaminoglycan (GAG) analog, to develop a novel bioMaterial to support chondrogenesis. Chitosan is a polycationic repeating monosaccharide of beta-1,4-linked glucosamine monomers with randomly located N-acetyl glucosamine units. Chitosan may be combined with the polyanionic CSA such that ionic crosslinking results in hydrogel formation. Bovine primary articular chondrocytes, when seeded onto a thin layer of CSA-chitosan, form discrete, focal adhesions to the Material and maintain many characteristics of the differentiated chondrocytic phenotype, including round morphology, limited mitosis, collagen type II, and proteoglycan production. Our findings suggest CSA-chitosan may be well suited as a carrier Material for the transplant of autologous chondrocytes or as a scaffold for the tissue engineering of cartilage-like tissue.
Tetsuo Asakura - One of the best experts on this subject based on the ideXlab platform.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:OBJECTIVE: There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those <5 mm in diameter, are associated with a high incidence of thrombosis. Fibroin is a Biodegradable protein derived from silk. Silk fibroin from Bombyx mori provides an antithrombotic surface and serves as a scaffold for various cell types in tissue engineering. We evaluated the potential of fibroin to generate a vascular prosthesis for small arteries. METHODS: A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. RESULTS: The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P < .01). Endothelial cells and smooth muscle cells (SMCs) migrated into the fibroin graft early after implantation and became organized into endothelial and medial layers, as determined by anti-CD31 and anti-alpha-smooth muscle actin immunostaining. The total number of SMCs increased 1.6-fold from 1 month to 3 months. Vasa vasorum also formed in the adventitia. Sirius red staining of the fibroin grafts revealed that the content of collagen significantly increased at 1 year after implantation, with a decrease in fibroin content. GFP-positive cells contributed to organization of a smooth muscle layer. CONCLUSIONS: Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.
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long term patency of small diameter vascular graft made from fibroin a silk based Biodegradable Material
Journal of Vascular Surgery, 2010Co-Authors: Soichiro Enomoto, Makoto Sumi, Chiyuki Takabayashi, Yasumoto Nakazawa, Kan Kajimoto, Rui Takahashi, Tetsuo Asakura, Masataka SataAbstract:Objective There is an increasing need for vascular grafts in the field of surgical revascularization. However, smaller vascular grafts made from synthetic bioMaterials, particularly those Methods A small vessel with three layers was woven from silk fibroin thread. These fibroin-based grafts (1.5 mm diameter, 10 mm length) were implanted into the abdominal aorta of 10- to 14-week-old male Sprague-Dawley rats by end-to-end anastomosis. Polytetrafluoroethylene (PTFE)-based grafts were used as the control. To investigate the origin of the cells in the neointima and media, bone marrow transplantation was performed from green fluorescent protein (GFP) rats to wild-type rats. Results The patency of fibroin grafts at 1 year after implantation was significantly higher than that of PTFE grafts (85.1% vs 30%, P Conclusions Small-diameter fibroin-based vascular grafts have excellent long-term patency. Bone marrow-derived cells contribute to vascular remodeling after graft implantation. Fibroin might be a promising Material to engineer vascular prostheses for small arteries.