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T G Van Kooten - One of the best experts on this subject based on the ideXlab platform.

  • A long-termin vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2020
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc

  • a long term in vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: B Van Minnen, M B M Van Leeuwen, Boudewijn Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc.

  • short term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1 4 butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and e-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated.

  • Short-term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1,4-butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and ε-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated. In vitro , the mass loss was 3.4% after twelve weeks. In the cytotoxicity tests the PU caused no abnormal growth behaviour, nor morphological changes or inhibition in metabolic activity. The in vivo studies showed no toxic tissue response to the PU. Connective tissue ingrowth, accompanied by vascular ingrowth was complete at twelve weeks. In vivo degradation had started within four to twelve weeks. In conclusion, the PU shows a good in vitro and in vivo biocompatibility in these short-term experiments.

William R. Wagner - One of the best experts on this subject based on the ideXlab platform.

  • thiol click modification of cyclic disulfide containing Biodegradable Polyurethane urea elastomers
    Biomacromolecules, 2015
    Co-Authors: Jun Fang, Sangho Ye, Jing Wang, Ting Zhao, Xiumei Mo, William R. Wagner
    Abstract:

    Although the thiol click reaction is an attractive tool for postpolymerization modification of thiolmers, thiol groups are easily oxidized, limiting the potential for covalent immobilization of bioactive molecules. In this study, a series of Biodegradable Polyurethane elastomers incorporating stable cyclic disulfide groups was developed and characterized. These poly(ester urethane)urea (PEUU-SS) polymers were based on polycaprolactone diol (PCL), oxidized dl-dithiothreitol (O-DTT), lysine diisocyanate (LDI), or butyl diisocyanate (BDI), with chain extension by putrescine. The ratio of O-DTT:PCL was altered to investigate different levels of potential functionalization. PEG acrylate was employed to study the mechanism and availability of both bulk and surface click modification of PEUU-SS polymers. All synthesized PEUU-SS polymers were elastic with breaking strengths of 38–45 MPa, while the PEUU-SS(LDI) polymers were more amorphous, possessing lower moduli and relatively small permanent deformations versus...

  • Biodegradable Polyurethane ureas with variable polyester or polycarbonate soft segments effects of crystallinity molecular weight and composition on mechanical properties
    Biomacromolecules, 2011
    Co-Authors: Yi Hong, Devin M. Nelson, Joseph E. Pichamuthu, Cory E. Leeson, William R. Wagner
    Abstract:

    Biodegradable Polyurethane urea (PUU) elastomers are ideal candidates for fabricating tissue engineering scaffolds with mechanical properties akin to strong and resilient soft tissues. PUU with a crystalline poly(e-caprolactone) (PCL) macrodiol soft segment (SS) showed good elasticity and resilience at small strains (<50%) but showed poor resilience under large strains because of stress-induced crystallization of the PCL segments, with a permanent set of 677 ± 30% after tensile failure. To obtain softer and more resilient PUUs, we used noncrystalline poly(trimethylene carbonate) (PTMC) or poly(δ-valerolactone-co-e-caprolactone) (PVLCL) macrodiols of different molecular weights as SSs that were reacted with 1,4-diisocyanatobutane and chain extended with 1,4-diaminobutane. Mechanical properties of the PUUs were characterized by tensile testing with static or cyclic loading and dynamic mechanical analysis. All of the PUUs synthesized showed large elongations at break (800–1400%) and high tensile strength (30...

  • Biodegradable Polyurethane Ureas with Variable Polyester or Polycarbonate Soft Segments: Effects of Crystallinity, Molecular Weight, and Composition on Mechanical Properties
    Biomacromolecules, 2011
    Co-Authors: Zuwei Ma, Yi Hong, Devin M. Nelson, Joseph E. Pichamuthu, Cory E. Leeson, William R. Wagner
    Abstract:

    Biodegradable Polyurethane urea (PUU) elastomers are ideal candidates for fabricating tissue engineering scaffolds with mechanical properties akin to strong and resilient soft tissues. PUU with a crystalline poly(e-caprolactone) (PCL) macrodiol soft segment (SS) showed good elasticity and resilience at small strains (

  • Controlled Release of IGF-1 and HGF from a Biodegradable Polyurethane Scaffold
    Pharmaceutical Research, 2011
    Co-Authors: Devin M. Nelson, Zuwei Ma, Priya R. Baraniak, Jianjun Guan, N. Scott Mason, William R. Wagner
    Abstract:

    Purpose Biodegradable elastomers, which can possess favorable mechanical properties and degradation rates for soft tissue engineering applications, are more recently being explored as depots for biomolecule delivery. The objective of this study was to synthesize and process Biodegradable, elastomeric poly(ester urethane)urea (PEUU) scaffolds and to characterize their ability to incorporate and release bioactive insulin-like growth factor–1 (IGF-1) and hepatocyte growth factor (HGF). Methods Porous PEUU scaffolds made from either 5 or 8 wt% PEUU were prepared with direct growth-factor incorporation. Long-term in vitro IGF-1 release kinetics were investigated in saline or saline with 100 units/ml lipase to simulate in vivo degradation. Cellular assays were used to confirm released IGF-1 and HGF bioactivity. Results IGF-1 release into saline occurred in a complex multi-phasic manner for up to 440 days. Scaffolds generated from 5 wt% PEUU delivered protein faster than 8 wt% scaffolds. Lipase-accelerated scaffold degradation led to delivery of >90% protein over 9 weeks for both polymer concentrations. IGF-1 and HGF bioactivity in the first 3 weeks was confirmed. Conclusions The capacity of a Biodegradable elastomeric scaffold to provide long-term growth-factor delivery was demonstrated. Such a system might provide functional benefit in cardiovascular and other soft tissue engineering applications.

  • controlled release of igf 1 and hgf from a Biodegradable Polyurethane scaffold
    Pharmaceutical Research, 2011
    Co-Authors: Devin M. Nelson, Priya R. Baraniak, Jianjun Guan, Scott N Mason, William R. Wagner
    Abstract:

    Purpose Biodegradable elastomers, which can possess favorable mechanical properties and degradation rates for soft tissue engineering applications, are more recently being explored as depots for biomolecule delivery. The objective of this study was to synthesize and process Biodegradable, elastomeric poly(ester urethane)urea (PEUU) scaffolds and to characterize their ability to incorporate and release bioactive insulin-like growth factor–1 (IGF-1) and hepatocyte growth factor (HGF).

B Van Minnen - One of the best experts on this subject based on the ideXlab platform.

  • A long-termin vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2020
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc

  • a long term in vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: B Van Minnen, M B M Van Leeuwen, Boudewijn Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc.

  • short term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1 4 butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and e-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated.

  • Short-term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1,4-butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and ε-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated. In vitro , the mass loss was 3.4% after twelve weeks. In the cytotoxicity tests the PU caused no abnormal growth behaviour, nor morphological changes or inhibition in metabolic activity. The in vivo studies showed no toxic tissue response to the PU. Connective tissue ingrowth, accompanied by vascular ingrowth was complete at twelve weeks. In vivo degradation had started within four to twelve weeks. In conclusion, the PU shows a good in vitro and in vivo biocompatibility in these short-term experiments.

M B M Van Leeuwen - One of the best experts on this subject based on the ideXlab platform.

  • A long-termin vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2020
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc

  • a long term in vitro biocompatibility study of a Biodegradable Polyurethane and its degradation products
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: B Van Minnen, M B M Van Leeuwen, Boudewijn Stegenga, T G Van Kooten
    Abstract:

    The biological safety of degradation products from degradable biomaterials is very important. In this study a new method is proposed to test the cytotoxicity of these degradation products with the aim to save time, laboratory animals, and research funds. A Biodegradable Polyurethane (PU) foam was subjected to this test method. The PU had soft segments of DL-lactide/is an element of-caprolactone and hard segments synthesized from butanediol and 1,4-butane-diiosocyanate. Copolymer foams without urethane segments, consisting of DL-lactide/is an element of-caprolactone, were tested as well. Accumulated degradation products were collected by degrading the foams in distilled water at 60 degrees C up to 52 weeks. Cell-culture medium was prepared from powder medium with this water. In different tests the cytotoxicity of this medium was established. The first signs of cytotoxicity were observed after 3-5 weeks of degradation. This accounts for both materials and reestablishes the good short-term biocompatibility of these materials. The PU showed more toxicity toward the end stages of degradation in comparison with the copolymer. This is probably related to the accumulation of degradation products of the urethane segments. In the in vivo situation the degradation of the PU and the metabolism and excretion of degradation products may differ. Therefore, long-term in vivo studies will have to establish whether these in vitro results are representative for the in vivo behavior of the degrading PU. (c) 2005 Wiley Periodicals, Inc.

  • short term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1 4 butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and e-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated.

  • Short-term in vitro and in vivo biocompatibility of a Biodegradable Polyurethane foam based on 1,4-butanediisocyanate
    Journal of Materials Science: Materials in Medicine, 2005
    Co-Authors: B Van Minnen, M B M Van Leeuwen, B Stegenga, J Zuidema, C E Hissink, T G Van Kooten
    Abstract:

    In this study short-term in vitro and in vivo biocompatibility apects of a Biodegradable Polyurethane (PU) foam were evaluated. The PU consists of hard urethane segments and amorphous soft segments based on a copolyester of dl-lactide and ε-caprolactone. The urethane segments are of uniform length and synthesized with 1,4-butanediisocyanate. The foam has good mechanical properties and will be used for tissue regeneration applications. Degradation tests were carried out in a buffer solution for twelve weeks. Cytotoxicity was determined using extract and direct contact test methods with incubation periods varying form 24 to 72 h. The foam was implanted subcutaneously for one, four and twelve weeks and the tissue response to the material was histologically evaluated. In vitro , the mass loss was 3.4% after twelve weeks. In the cytotoxicity tests the PU caused no abnormal growth behaviour, nor morphological changes or inhibition in metabolic activity. The in vivo studies showed no toxic tissue response to the PU. Connective tissue ingrowth, accompanied by vascular ingrowth was complete at twelve weeks. In vivo degradation had started within four to twelve weeks. In conclusion, the PU shows a good in vitro and in vivo biocompatibility in these short-term experiments.

Shan-hui Hsu - One of the best experts on this subject based on the ideXlab platform.

  • 3D bioprinting of neural stem cell-laden thermoresponsive Biodegradable Polyurethane hydrogel and potential in central nervous system repair
    Biomaterials, 2015
    Co-Authors: Fu Yu Hsieh, Hsin Hua Lin, Shan-hui Hsu
    Abstract:

    The 3D bioprinting technology serves as a powerful tool for building tissue in the field of tissue engineering. Traditional 3D printing methods involve the use of heat, toxic organic solvents, or toxic photoinitiators for fabrication of synthetic scaffolds. In this study, two thermoresponsive water-based Biodegradable Polyurethane dispersions (PU1 and PU2) were synthesized which may form gel near 37 °C without any crosslinker. The stiffness of the hydrogel could be easily fine-tuned by the solid content of the dispersion. Neural stem cells (NSCs) were embedded into the Polyurethane dispersions before gelation. The dispersions containing NSCs were subsequently printed and maintained at 37 °C. The NSCs in 25-30% PU2 hydrogels (~680-2400 Pa) had excellent proliferation and differentiation but not in 25-30% PU1 hydrogels. Moreover, NSC-laden 25-30% PU2 hydrogels injected into the zebrafish embryo neural injury model could rescue the function of impaired nervous system. However, NSC-laden 25-30% PU1 hydrogels only showed a minor repair effect in the zebrafish model. In addition, the function of adult zebrafish with traumatic brain injury was rescued after implantation of the 3D-printed NSC-laden 25% PU2 constructs. Therefore, the newly developed 3D bioprinting technique involving NSCs embedded in the thermoresponsive Biodegradable Polyurethane ink offers new possibilities for future applications of 3D bioprinting in neural tissue engineering.

  • The effect of elastic Biodegradable Polyurethane electrospun nanofibers on the differentiation of mesenchymal stem cells
    Colloids and Surfaces B: Biointerfaces, 2014
    Co-Authors: Yi-chia Kuo, Shih-chieh Hung, Shan-hui Hsu
    Abstract:

    Biodegradable Polyurethane (PU) was synthesized based on using poly(ɛ-caprolactone) (PCL) as the soft segment. Fibers in different diameters (200-400nm, 600-800nm, and 1.4-1.6μm) were then made by electrospinning PU solution in N,N-dimethylacetamide and 2,2,2-trifluoroethanol. Human bone marrow derived mesenchymal stem cells (hMSCs) in the form of single dispersed cells or aggregates were seeded on the electrospun meshes for evaluation of cell behavior. Differentiation experiments showed that hMSC aggregates on electrospun fibers had greater differentiation capacities than single cells. Besides, nanofibers of 200-400nm diameters significantly promoted the osteogenic and chondrogenic differentiation of hMSCs than fibers of the other diameters. The effect of substrate elasticity was further elucidated by comparing cell behaviors on the nanofibers of PCL-based PU and those of pure PCL. The more elastic PU nanofibers demonstrated more osteogenic and chondrogenic induction potential than PCL electrospun fibers. We suggested that the elastic nanofibers seeded with hMSC aggregates may be advantageous for cartilage and bone tissue engineering.

  • Synthesis and 3D Printing of Biodegradable Polyurethane elastomer by a water-based process for cartilage tissue engineering applications
    Advanced Healthcare Materials, 2014
    Co-Authors: Kun Che Hung, Ching Shiow Tseng, Shan-hui Hsu
    Abstract:

    Biodegradable materials that can undergo degradation in vivo are commonly employed to manufacture tissue engineering scaffolds, by techniques including the customized 3D printing. Traditional 3D printing methods involve the use of heat, toxic organic solvents, or toxic photoinitiators for fabrication of synthetic scaffolds. So far, there is no investigation on water-based 3D printing for synthetic materials. In this study, the water dispersion of elastic and Biodegradable Polyurethane (PU) nanoparticles is synthesized, which is further employed to fabricate scaffolds by 3D printing using polyethylene oxide (PEO) as a viscosity enhancer. The surface morphology, degradation rate, and mechanical properties of the water-based 3D-printed PU scaffolds are evaluated and compared with those of polylactic-co-glycolic acid (PLGA) scaffolds made from the solution in organic solvent. These scaffolds are seeded with chondrocytes for evaluation of their potential as cartilage scaffolds. Chondrocytes in 3D-printed PU scaffolds have excellent seeding efficiency, proliferation, and matrix production. Since PU is a category of versatile materials, the aqueous 3D printing process developed in this study is a platform technology that can be used to fabricate devices for biomedical applications.