The Experts below are selected from a list of 1161 Experts worldwide ranked by ideXlab platform
Bradford A Woodworth - One of the best experts on this subject based on the ideXlab platform.
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sinupret activates cftr and tmem16a dependent transepithelial chloride transport and improves indicators of mucociliary clearance
PLOS ONE, 2014Co-Authors: Shaoyan Zhang, Eric J Sorscher, Daniel Skinner, Stephen B Hicks, Mark O Bevensee, Ahmed Lazrak, Sadis Matalon, Carmel M Mcnicholas, Bradford A WoodworthAbstract:Introduction We have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl−) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this Bioflavonoid.
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the Bioflavonoid compound sinupret stimulates transepithelial chloride transport in vitro and in vivo
Laryngoscope, 2010Co-Authors: Frank W Virgin, Shaoyan Zhang, Daniel Schuster, Christopher Azbell, James A Fortenberry, Eric J Sorscher, Bradford A WoodworthAbstract:Objectives/Hypothesis: Dehydration of airway surface liquid (ASL) disrupts normal mucociliary clearance in sinonasal epithelium leading to chronic rhinosinusitis. Abnormal chloride (Cl - ) transport is one mechanism that contributes to this disorder, as demonstrated by the disease cystic fibrosis. Identifying safe compounds that stimulate transepithelial Cl - transport is critical to improving hydration of the ASL and promoting mucociliary transport. Sinupret (Bionorica, LLC, San Clemente, CA), a combination of naturally occurring Bioflavonoids, is a widely used treatment for respiratory ailments in Europe. However, the effects of Sinupret on target respiratory epithelium have yet to be fully investigated. The present study evaluated the mechanisms underlying this Bioflavonoid therapeutic on transepithelial Cl - transport in respiratory epithelium. Study Design: In vitro and in vivo investigation. Methods: Well characterized murine nasal septal epithelial (MNSE) cultures, and murine nasal potential difference (NPD) techniques were used to evaluate the effects of Sinupret on Cl - secretion. Results: The change in Sinupret-stimulated current (Δ I SC expressed as μA/cm 2 ) in MNSE, representing Cl - secretion, was significantly increased when compared to controls (19.04 ± 1.67 μA/cm 2 vs. 1.8 ± 0.35 μA/cm 2 , respectively; P = .00005). Transepithelial Cl - transport measured in the murine NPD in vivo assay (n = 42) was also significantly enhanced when compared to controls (—0.8 mV vs. -0.9 mV; P = .0004). Importantly, Sinupret-stimulated Cl - transport was substantially more robust in vivo than forskolin, a compound among the strongest known cystic fibrosis transmembrane conductance regulator activators (-3.8 mV vs. -1.65 mV; P = .01). Conclusions: Sinupret strongly activates transepithelial Cl - secretion through a mechanism known to hydrate the ASL of respiratory epithelium. This is one means by which the medication is likely to exert therapeutic benefit.
Shrinivas K Kulkarni - One of the best experts on this subject based on the ideXlab platform.
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quercetin a Bioflavonoid attenuates haloperidol induced orofacial dyskinesia
Neuropharmacology, 2003Co-Authors: Pattipati S Naidu, Amanpreet Singh, Shrinivas K KulkarniAbstract:Abstract Chronic treatment with neuroleptics leads to the development of abnormal orofacial movements described as vacuous chewing movements (VCMs) in rats. Vacuous chewing movements in rodents are widely accepted as one of the animal models of tardive dyskinesia. Oxidative stress and the products of lipid peroxidation are implicated in the pathophysiology of various neurological disorders including tardive dyskinesia. In the present study chronic haloperidol (1.0 mg kg −1 for 21 days) treatment induced vacuous chewing movements and tongue protrusions in rats. Co-administration of quercetin, a Bioflavonoid, dose dependently (25–100 mg kg −1 ) reduced haloperidol-induced vacuous chewing movements and tongue protrusions. Biochemical analysis revealed that chronic haloperidol treatment induces lipid peroxidation and decreases the glutathione (GSH) levels in the forebrains of rats. The antioxidant defense enzymes, superoxide dismutase (SOD) and catalase were also decreased due to chronic haloperidol treatment. Co-administration of quercetin (25–100 mg kg −1 ) significantly reduced the lipid peroxidation and restored the decreased glutathione levels in these animals. Further quercetin (50–100 mg kg −1 ) also reversed the haloperidol-induced decrease in forebrain SOD and catalase levels in rats. The major findings of the present study suggested that oxidative stress plays a significant role in neuroleptic-induced orofacial dyskinesia and quercetin co-administration reverses these behavioral and biochemical changes. Quercetin, a naturally occurring Bioflavonoid could prove to be a useful agent in neuroleptic-induced orofacial dyskinesia.
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reversal of haloperidol induced orofacial dyskinesia by quercetin a Bioflavonoid
Psychopharmacology, 2003Co-Authors: Pattipati S Naidu, Amanpreet Singh, Shrinivas K KulkarniAbstract:Rationale Tardive dyskinesia is a serious neurological syndrome associate with long-term administration of neuroleptics to humans and experimental animals. It may be caused by loss of dopaminergic cells, due to free radicals as a product of high synaptic dopamine levels. Quercetin is a Bioflavonoid with strong antioxidant properties.
Shaoyan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance
2016Co-Authors: Shaoyan Zhang, Eric J Sorscher, Daniel Skinner, Stephen B Hicks, Mark O Bevensee, Ahmed Lazrak Sadis MatalonAbstract:Introduction: We have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl2) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this Bioflavonoid. Methods: Primary murine nasal septal epithelial (MNSE) [wild type (WT) and transgenic CFTR2/2], human sinonasa
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sinupret activates cftr and tmem16a dependent transepithelial chloride transport and improves indicators of mucociliary clearance
PLOS ONE, 2014Co-Authors: Shaoyan Zhang, Eric J Sorscher, Daniel Skinner, Stephen B Hicks, Mark O Bevensee, Ahmed Lazrak, Sadis Matalon, Carmel M Mcnicholas, Bradford A WoodworthAbstract:Introduction We have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl−) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this Bioflavonoid.
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the Bioflavonoid compound sinupret stimulates transepithelial chloride transport in vitro and in vivo
Laryngoscope, 2010Co-Authors: Frank W Virgin, Shaoyan Zhang, Daniel Schuster, Christopher Azbell, James A Fortenberry, Eric J Sorscher, Bradford A WoodworthAbstract:Objectives/Hypothesis: Dehydration of airway surface liquid (ASL) disrupts normal mucociliary clearance in sinonasal epithelium leading to chronic rhinosinusitis. Abnormal chloride (Cl - ) transport is one mechanism that contributes to this disorder, as demonstrated by the disease cystic fibrosis. Identifying safe compounds that stimulate transepithelial Cl - transport is critical to improving hydration of the ASL and promoting mucociliary transport. Sinupret (Bionorica, LLC, San Clemente, CA), a combination of naturally occurring Bioflavonoids, is a widely used treatment for respiratory ailments in Europe. However, the effects of Sinupret on target respiratory epithelium have yet to be fully investigated. The present study evaluated the mechanisms underlying this Bioflavonoid therapeutic on transepithelial Cl - transport in respiratory epithelium. Study Design: In vitro and in vivo investigation. Methods: Well characterized murine nasal septal epithelial (MNSE) cultures, and murine nasal potential difference (NPD) techniques were used to evaluate the effects of Sinupret on Cl - secretion. Results: The change in Sinupret-stimulated current (Δ I SC expressed as μA/cm 2 ) in MNSE, representing Cl - secretion, was significantly increased when compared to controls (19.04 ± 1.67 μA/cm 2 vs. 1.8 ± 0.35 μA/cm 2 , respectively; P = .00005). Transepithelial Cl - transport measured in the murine NPD in vivo assay (n = 42) was also significantly enhanced when compared to controls (—0.8 mV vs. -0.9 mV; P = .0004). Importantly, Sinupret-stimulated Cl - transport was substantially more robust in vivo than forskolin, a compound among the strongest known cystic fibrosis transmembrane conductance regulator activators (-3.8 mV vs. -1.65 mV; P = .01). Conclusions: Sinupret strongly activates transepithelial Cl - secretion through a mechanism known to hydrate the ASL of respiratory epithelium. This is one means by which the medication is likely to exert therapeutic benefit.
Wagner Vilegas - One of the best experts on this subject based on the ideXlab platform.
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application of preparative high speed counter current chromatography for the separation of flavonoids from the leaves of byrsonima crassa niedenzu ik
Journal of Chromatography A, 2004Co-Authors: Miriam Sannomiya, Clenilson Martins Rodrigues, Roberta Gomes Coelho, Lourdes Campaner Dos Santos, Clelia Akiko Hirumalima, Alba Regina Monteiro Souza Brito, Wagner VilegasAbstract:Abstract The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate– n -propanol–water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3- O -α- l -arabinoside, quercetin-3- O -β- d -galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3- O -α- l -arabinoside (95 mg), quercetin-3- O -β- d -galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1 H NMR, 13 C NMR and UV analyses.
Eric J Sorscher - One of the best experts on this subject based on the ideXlab platform.
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Sinupret Activates CFTR and TMEM16A-Dependent Transepithelial Chloride Transport and Improves Indicators of Mucociliary Clearance
2016Co-Authors: Shaoyan Zhang, Eric J Sorscher, Daniel Skinner, Stephen B Hicks, Mark O Bevensee, Ahmed Lazrak Sadis MatalonAbstract:Introduction: We have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl2) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this Bioflavonoid. Methods: Primary murine nasal septal epithelial (MNSE) [wild type (WT) and transgenic CFTR2/2], human sinonasa
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sinupret activates cftr and tmem16a dependent transepithelial chloride transport and improves indicators of mucociliary clearance
PLOS ONE, 2014Co-Authors: Shaoyan Zhang, Eric J Sorscher, Daniel Skinner, Stephen B Hicks, Mark O Bevensee, Ahmed Lazrak, Sadis Matalon, Carmel M Mcnicholas, Bradford A WoodworthAbstract:Introduction We have previously demonstrated that Sinupret, an established treatment prescribed widely in Europe for respiratory ailments including rhinosinusitis, promotes transepithelial chloride (Cl−) secretion in vitro and in vivo. The present study was designed to evaluate other indicators of mucociliary clearance (MCC) including ciliary beat frequency (CBF) and airway surface liquid (ASL) depth, but also investigate the mechanisms that underlie activity of this Bioflavonoid.
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the Bioflavonoid compound sinupret stimulates transepithelial chloride transport in vitro and in vivo
Laryngoscope, 2010Co-Authors: Frank W Virgin, Shaoyan Zhang, Daniel Schuster, Christopher Azbell, James A Fortenberry, Eric J Sorscher, Bradford A WoodworthAbstract:Objectives/Hypothesis: Dehydration of airway surface liquid (ASL) disrupts normal mucociliary clearance in sinonasal epithelium leading to chronic rhinosinusitis. Abnormal chloride (Cl - ) transport is one mechanism that contributes to this disorder, as demonstrated by the disease cystic fibrosis. Identifying safe compounds that stimulate transepithelial Cl - transport is critical to improving hydration of the ASL and promoting mucociliary transport. Sinupret (Bionorica, LLC, San Clemente, CA), a combination of naturally occurring Bioflavonoids, is a widely used treatment for respiratory ailments in Europe. However, the effects of Sinupret on target respiratory epithelium have yet to be fully investigated. The present study evaluated the mechanisms underlying this Bioflavonoid therapeutic on transepithelial Cl - transport in respiratory epithelium. Study Design: In vitro and in vivo investigation. Methods: Well characterized murine nasal septal epithelial (MNSE) cultures, and murine nasal potential difference (NPD) techniques were used to evaluate the effects of Sinupret on Cl - secretion. Results: The change in Sinupret-stimulated current (Δ I SC expressed as μA/cm 2 ) in MNSE, representing Cl - secretion, was significantly increased when compared to controls (19.04 ± 1.67 μA/cm 2 vs. 1.8 ± 0.35 μA/cm 2 , respectively; P = .00005). Transepithelial Cl - transport measured in the murine NPD in vivo assay (n = 42) was also significantly enhanced when compared to controls (—0.8 mV vs. -0.9 mV; P = .0004). Importantly, Sinupret-stimulated Cl - transport was substantially more robust in vivo than forskolin, a compound among the strongest known cystic fibrosis transmembrane conductance regulator activators (-3.8 mV vs. -1.65 mV; P = .01). Conclusions: Sinupret strongly activates transepithelial Cl - secretion through a mechanism known to hydrate the ASL of respiratory epithelium. This is one means by which the medication is likely to exert therapeutic benefit.