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Biogenesis
The Experts below are selected from a list of 141654 Experts worldwide ranked by ideXlab platform
Hugo W Moser – 1st expert on this subject based on the ideXlab platform
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peroxisome Biogenesis disorders
Biochimica et Biophysica Acta, 2006Co-Authors: Steven J Steinberg, Nancy Braverman, Gabriele Dodt, Hugo W Moser, Gerald V Raymond, Ann B MoserAbstract:Defects in PEX genes impair peroxisome assembly and multiple metabolic pathways confined to this organelle, thus providing the biochemical and molecular bases of the peroxisome Biogenesis disorders (PBD). PBD are divided into two types—Zellweger syndrome spectrum (ZSS) and rhizomelic chondrodysplasia punctata (RCDP). Biochemical studies performed in blood and urine are used to screen for the PBD. DNA testing is possible for all of the disorders, but is more challenging for the ZSS since 12 PEX genes are known to be associated with this spectrum of PBD. In contrast, PBD-RCDP is associated with defects in the PEX7 gene alone. Studies of the cellular and molecular defects in PBD patients have contributed significantly to our understanding of the role of each PEX gene in peroxisome assembly.
Xi Long – 2nd expert on this subject based on the ideXlab platform
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A mutation in porcine pre-miR-15b alters the Biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b.
PloS one, 2017Co-Authors: Wenyang Sun, Jing Lan, Lei Chen, Jinjie Qiu, Zonggang Luo, Mingzhou Li, Jinyong Wang, Jiugang Zhao, Tinghuan Zhang, Xi LongAbstract:MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their Biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs Biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA Biogenesis.
Wenyang Sun – 3rd expert on this subject based on the ideXlab platform
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A mutation in porcine pre-miR-15b alters the Biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b.
PloS one, 2017Co-Authors: Wenyang Sun, Jing Lan, Lei Chen, Jinjie Qiu, Zonggang Luo, Mingzhou Li, Jinyong Wang, Jiugang Zhao, Tinghuan Zhang, Xi LongAbstract:MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their Biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs Biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA Biogenesis.